집파리유충 hemolymph 중신형의 anti-fungal peptides의 분리정제

Jian-Wei Wu,Li-juan Gu,Xiao-Qing Su,성창근 한국생명과학회 2006 생명과학회지 Vol.16 No.3

To isolate and purify anti-fungal active substances from immunized housefly (Musca domestica), low dose of Candida albicans was injected into the larvae of the housefly to induce the appearance of potent anti-fungal active substances in the hemolymph. This purification work was performed by the routine isolation and purification processes of protein, namely, solid phase extraction (SPE), SDS-PAGE electrophoresis, HPLC purification. Three 4~16 kDa peptides which exhibited antifungal activity against Candida albican and other fungi were isolated from induced hemolymph. Consequently, further anti-fungal activity study showed that these three peptides were different either in molecular weight or in anti-fungal activity. All isolated substances were proved to be active and resistant to high-temperature. It was deduced that these peptides isolated from induced housefly were novel members of the insect defensin family and they were inducible. 유도된 집파리유통 hemolymph중에서 Candida albicans의 3가지 anti-fungal peptides를 분리하였다. 3개 anti-fungal peptides는 분자량이 4-16 kDa 사이의 분명한 구별이 있을 뿐만 아니라, 각 peptide는 anti-fungal peptides 작용이 있었다. 이들 peptide의 공통 특징은 모두 열을 받은 뒤 활성이 변하지 않는 비교적 강한 내열성을 보여주었다.

찔레뿌리 초임계 추출물의 항균, 항산화 및 항염증 활성

고영심,최선은 한국식품저장유통학회 2018 한국식품저장유통학회지 Vol.25 No.2

The roots of Rosa multiflora Thunberg have been used in traditional oriental medicine as remedies for rheumatic arthralgia and scabies. In this study, the anti-fungal, anti-oxidant, and anti-inflammatory activities of a supercritical extract of Rosa multiflora root were investigated in vitro. To investigate the anti-oxidant and anti-inflammatory effects of the supercritical extract, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, and the inhibition of nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were examined, respectively. In addition, the anti-fungal activities of the extract were assessed. The results showed a concentration-dependent, increase in ABTS radical scavenging activity. The supercritical fluid extracts of Rosa multiflora root exhibited low toxicity to RAW 264.7 cells at 100 µg/mL the highest concentration tested. Cells stimulated with LPS produced more nitric oxide than normal control cells; however, cells treated with the supercritical fluid extract decreased this production in a concentration-dependent manner. Finally, the supercritical fluid extracts showed significant anti-fungal activity. These results suggest that extracts of the roots of Rosa multiflora might be used to develop potent anti-fungal, anti-oxidant, and anti-inflammatory agents, and may be useful as ingredients for related new functional cosmetic materials.

연구논문 : 오이풀 추출물의 비듬균에 대한 항진균 효과 및 RAW 264.7 세포에서의 항염증 효과

유민정 ( Min Jeong Ryu ),임요섭 ( Yo Sup Rim ),최다인 ( Da In Choi ),이숙영 ( Sook Young Lee ) 한국미용학회 2011 한국미용학회지 Vol.17 No.2

In this study, we analyzed the anti-fungal activity against Malassezia furfur, anti-oxidative activity and anti-inflammatory activity of Sanguisorba officinalis extracts. The ethanol extract of S. officinalis was sequentially fractionated with hexane, ethyl acetate, butanol and water. The cell viability was measured by MTT assay. Nitric oxide (NO) production was measured by Griess reagent assay. Anti-fungal activity was examined by paper disc method. The total phenolic contents and flavonoid contents were determined in all the samples, in the following order: ethanol > ethyl acetate > butanol > water > n-hexane. The ethyl acetate fraction showed high inhibition activity to DPPH scavenging radical activity at 500 μg/mL (96%) and more strongly inhibited NO productions compared with other fractions. Also, the ethyl acetate fraction showed the highest anti-fungal activity. According to the results above, it was summarized that the ethyl acetate fraction of S. officinalis have anti-oxidative, anti-fungal and anti-inflammatory activity in M. furfur or macrophage. It would be proposed that ethyl acetate fraction of S. officinalis can become a new cosmetic materials.

느릅나무 초임계 추출물의 항균, 항산화 및 항염증 활성

서주희,이용조,조영익,고정윤,문명재,박광현,최선은 한국융합학회 2018 한국융합학회논문지 Vol.9 No.8

천연물 유래 난치성 피부질환 기능성 소재를 탐색하기 위해 느릅나무 가지를 초임계 유체 기술을 활용하여 추출을 수행하였고, 확보된 느릅나무 초임계 추출물을 수종의 피부상재균에 대하여 항균력 실험을 실시하였고, 모든 균종에 대해서 항균력이 확인이 되었으며, ABTS 라디컬 소거능과 NO 소거능 실험을 통해서 느릅나무 초임계 추출물의 항산화 활성과 항염증 활성의 우수한 활성이 확인이 되어서 산화적 스트레스 질환과 염증성 피부질환에 대해서 예방 및 치료 보완 기능성 소재로 개발이 가능성이 높다는 것을 알 수 있었다. 결론적으로 느릅나무 초임계 추출물은 피부상재균에 대한 항균활성, 라디컬 소거능에 의한 항산화 활성, 과 생성된 NO 소거능에 의한 항염증활성이 각각 확인이 되었다. 이 상의 결과로 느릅나무 초임계 추출물은 항균효능, 항산화효능, 항염증 효능을 갖는 기능성 원료로서의 가능성을 확인할 수 있었고, 향후 만성 염증 성 피부면역 질환 개선 및 치료 보조제 관련 의약품 또는 기능성 향장품 개발을 위한 기초 연구 자료가 될 것으로 사료된다. The Ulmus davidiana have been used in traditional oriental medicine as remedies for inflammation, ulcers, cancers, bacterial infections and scabies. In this study, the anti-fungal, anti-oxidant, and anti-inflammatory activitiesof a supercritical extract of U. davidiana were investigated in vitro. To explore the anti-oxidant and anti-inflammatory characteristics of the supercritical extract, ABTS radical scavenging activity, and the inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells were examined, respectively. In addition, the anti-fungal activities of the extract were assessed. The results showed a concentration-dependent increase in ABTS radical scavenging activity. Cells stimulated with LPS produced more nitric oxide than normal control cells; however, cells treated with the supercritical fluid extract decreased this production in a concentration-dependent manner. Finally, the supercritical fluid extracts showed significant anti-fungal activity. These results suggest that extracts of the U. davidiana might be used to develop potent anti-fungal, anti-oxidant, and anti-inflammatory agents, and may be useful as ingredients for related new functional cosmetic materials.

항균/항곰팡이/항알러지/항바이러스 기능의 자동차 헬스케어 내장재 개발

황진호(Jin Ho Hwang),장용수(YongSoo Chang),이복철(BockCheol Lee),이준석(JunSeok Lee) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11

In this study, as a study for implementing an automotive effectiveness of anti-microbial, anti-fungal, anti-allergen, anti-viral and for considering the operating mechanism based on the activating principle of the anti-substance by the addition of anti-substance to the interior parts of the vehicle such as head lining and carpets, was looking for ways to take advantage of the part. Selection of health-care parts that can exert the maximum effect in a room interior parts to it, and handle the claim for this various material to identify specific effects through the basic material properties verification, was considered to simultaneously satisfied the verification of health-care performance, the reliability of production molded parts and easiness of manufacturing method. In addition, the optimized evaluation method was appropriately selected to the automotive parts through anti-microbial, anti-fungal, anti-allergen and anti-virus performance review and assessment methods.

In vitro Anti-fungal Activity of Various Hydroxylated Fatty Acids Bioconverted by Pseudomonas aeruginosa PR3

Bajpai Vivek K.,Kim, Hak-Ryul,Kang, Sun-Chul The Korean Society for Applied Biological Chemistr 2006 Journal of Applied Biological Chemistry (J. Appl. Vol.49 No.4

The in vitro anti-fungal activity of hydroxylated fatty acids obtained from microbial conversion by Psuedomonas aeruginosa PR3 using ricinoleic acid(RA), eicosadienoic acid(EDA) and conjugated linoleic acid(CLA) as substrates, was investigated. Bioconverted hydroxylated fatty acids showed different anti-fungal activities potentials against the range of phytopathogenic fungi such as Botrytis cinerea, Rhizoctonia solani, Fusarium oxysporum, Sclerotonia sclerotiorum, Colletotricum capsici, Fusarium solani and Phytophthora capsici. RA and EDA showed up to 50% fungal mycelial inhibition at the concentration of $5{\mu}l\;ml^{-1}$. RA, EDA and CLA also exhibited anti-fungal activities with minimum inhibitory concentration(MIC), ranging from 500 to $1000{\mu}g\;ml^{-1}$. Screening was also carried out using varied concentrations of bioconverted RA and EDA for determining the anti-fungal effect on the spore germination of different fungi. Bioconverted RA and EDA showed a considerable degree of spore germination inhibition.

Anti-fungal action of bioconverted eicosapentaenoic acid (bEPA) against plant pathogens

Bajpai, V.K.,Shin, S.Y.,Kim, H.R.,Kang, S.C. Elsevier 2008 Industrial crops and products Vol.27 No.1

Bioconverted eicosapentaenoic acid (bEPA), obtained from Pseudomonas aeruginosa PR3, was assessed for its in vitro and in vivo anti-fungal potential. Mycelial growth inhibition of the tested plant pathogens (Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Fusarium solani, Phyptophthora capsici, Sclerotinia sclerotiorum and Colletotrichum capsici) was measured in vitro. bEPA at the concentration of 5μl/ml inhibited 52-60% fungal mycelial growth for all of the plant pathogens in vitro except S. sclerotiorum. Minimum inhibitory concentrations (MICs) of bEPA were found in the range of 250-500μg/ml. Also, bEPA had a detrimental effect on spore germination for all the tested plant pathogens. Three plant pathogenic fungi (F. oxysporum, P. capsici and C. capsici) were subjected to an in vivo anti-fungal screening. bEPA at the initial concentration of 3000μg/ml had a 100% anti-fungal effect against all of the tested plant pathogens. Concentrations of bEPA corresponding to 1500, 500 and 300μg/ml were applied to the plants and revealed promising anti-fungal effects, supporting bEPA as a potential anti-fungal agent.

오리나무 가지 초임계 추출물의 생리활성

하은주,최선은 한국생물공학회 2019 KSBB Journal Vol.34 No.3

The branches of Alnus japonica have been used inoriental traditional medicine practice for the formulation ofmedicines as the remedies for fever, hemorrhage, diarrhea,gastroenteric disorder, lymphatic disease, and cancers. In thisstudy, the anti-bacterial, anti-oxidant, anti-inflammatory, andanti-allergic activities of a supercritical extract of branches ofAlnus japonica (AJSCF) were investigated by in vitro assays. To investigate the antioxidant and anti-inflammatory effectsof a supercritical extract of branches of AJSCF, ABTS radicalscavenging activities and inhibitory activity against nitricoxide production in lipopolysaccharide (LPS) stimulated RAW264.7 cells were examined. Furthermore, anti-bacterial activitieswere examined. AJSCF on immunoglobulin E (IgE)-induced allergic inflammation in RBL-2H3 cells. In conclusion,it was observed that dose-dependent increase in AJSCFconcentration led to an increase in the ABTS radical scavengingactivity. The AJSCF showed low cytotoxicity at 50ug/mLconcentration, which was the highest concentration of theexamination. The cells stimulated with LPS produced moreNO than normal control cells; however, cells treated withsupercritical fluid extracts of branches of Alnus japonicainhibited NO production in a concentration-dependent manner. In addition, AJSCF showed the highest antifungal activityand concentration-dependent anti-allergic activity. Theresults suggest that AJSCF might be developed as a potentanti-fungal, anti-oxidant, anti-inflammatory, and anti-allergicagent for the production of novel and functional cosmeticmaterials.

Antimicrobial and antifungal effects of a green tea extract against vaginal pathogens

( Sang Hee Kim ),( Lan Sook Lee ),( Su Mi Bae ),( Sei Jun Han ),( Byung Rai Lee ),( Woong Shick Ahn ) 대한산부인과학회 2008 Journal of Womens Medicine Vol.1 No.1

Objective: Green tea extract (GTEs), including catechins and caffeine, have strong anti-microbial activity. Methods: We determined the anti-microbial and anti-fungal effects of a GTE on the vaginal pathogens, Proteus mirabilis (KCTC 2510), Streptococcus pyogenes (KCTC 3096), and Candida albicans (KCTC 7270). Results: The growth inhibitory effects of a GTE and the ethyl acetate fraction against P. mirabilis and S. pyogenes were stronger compared to the anti-microbial activity of the H2O fraction. Among the catechins, epigallocatechin gallate had the strongest anti-microbial activity. The growth of C. albicans was strongly inhibited by the methylene chloride fraction and caffeine; the anti-fungal activity of a GTE was due to caffeine. We also evaluated whether the in vitro activity of a GTE included purified solvent fractions stable to heat and pH changes. The stability of transtype catechins increased following heat treatment, but this did not significantly affect the anti-microbial activity. A GTE were stable over the pH range 4-10. The most active anti-microbial activity of a GTE occurred in an alkaline, rather than an acid environment, except for the anti-microbial activity against S. pyogenes and C. albicans, which was not influenced by pH. Conclusions: Taken together, the results of this study indicate that a GTE may be a valuable therapeutic agent for the treatment of vaginal pathogens.

은 나노 헤파 필터를 이용한 공기 부유 곰팡이 Cladosporium의 저감 및 ATP 생물 발광법 이용항 곰팡이성 신속평가

안지혜,황정호 한국실내환경학회 2013 한국실내환경학회지 Vol.10 No.3

Since airborne fungi have been known to aggravate indoor air quality, studies on developing anti-fungal filters increase recently. In this study, silver (Ag) nanoparticle was selected as anti-fungal agent. HEPA filter was coated with silver nanoparticles which were generated via spark discharge system operating at atmospheric pressure and temperature. The anti-fungal effect of the Ag-filter was evaluated with the conventional culture assay. When the number of Ag nano particle per a fungal particle in the filter was 1.91X106, the fungicidal efficiency was higher than 99%. As another anti-fungal test, ATP bioluminiscence detection method was also carried out and the results were correlated with those of the culture assay.