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국내산 백석면과 안소필라이트의 물리화학적 특성과 호흡기계 내 변화 연구
정용현 ( Yong Hyun Chung ),한정희 ( Jeong Hee Han ),강민구 ( Min Gu Kang ),김종규 ( Jong Kyu Kim ),양정선 ( Jeong Sun Yang ) 한국산업위생학회 2012 한국산업보건학회지 Vol.22 No.3
Objectives: To assess the hazard of Korea chrysotile and anthophylite, fibers were analyzed for their physicochemical properties by transmission electron microscope equipped with energy dispersive X-ray spectrometer (TEM-EDS). Methods: To evaluate the biopersistence of 2 domestic asbestos, Sprague-Dawely rats were exposed to 2 mg asbestos by intratracheal instillation. Each asbestos (chrysotile; 8,814,244 × 106 fibers/mg, average size 0.08 um × 4.39 um, anthophyllite ; 5,182 × 106 fibers/mg, average size 0.95 um × 7.29 um) were evaluated after a single intratracheal instillation. At times from 1 week to 4 weeks after exposure, the numbers of asbestos fivers in the bronchoalveolar lavage fluid and in the lung were calculated. Results: Anthophyllite fivers continuously have retained for 4 weeks but chrysotile fivers were rarely found at 4 weeks after exposure in the bronchoalveolar lavage fluid. Chrysotile fivers at 4 weeks after treatment were not observed but anthophyllite was easily observed in the lung with phase contrast microscopy. According to electron microscopic observation of asbestos in the lung, within 1 week after the administration of chrysotile fivers were decreased rapidly but anthophyllite fivers were very little change for 4 weeks. When chrysotile fivers have been lost Fe in 1 week, there were no significant changes in anthophyllite fivers in the lung. Conclusions: These findings indicate that after a long time exposure to chrysotile, asbestos bodies cannot be found in the bronchoalveolar lavage fluid.
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중량당 섬유수가 다른 국내산 석면이 폐 기능과 폐 조직에 미치는 영향 평가
정용현 ( Yong Hyun Chung ),한정희 ( Jeong Hee Han ),강민구 ( Min Gu Kang ),김종규 ( Jong Kyu Kim ),양정선 ( Jeong Sun Yang ) 한국산업위생학회 2012 한국산업보건학회지 Vol.22 No.4
Objectives: To evaluate the pulmonary toxicity of 2 Korea asbestos(chrysotile, anthophyllite), Sprague-Dawely rats were exposed to 2 mg domestic asbestos by intratracheal instillation(IT). Methods: Lung function of rats was analyzed by pressure transducer(MAX1320, Buxco Electronics, USA). The effects of 2 mg asbestos(chrysotile; 8,814,244 × 106 fibers/mg, average diameter 0.08 μm, average length 4.39 μm, anthophyllite; 5,182 × 106 fibers/mg, average diameter 0.95 μm, average length 7.29 μm) on pulmonary function and pathological changes were evaluated at after a single IT. Lung function and histopathological evaluation were assessed in 5 animals from each group at each time point. Results: Due to differences in fiber numbers, chrysotile induce marked lung pathology and lung function change than anthophyllite at the same mass dose. Chrysotile showed notable thickening of interstitial areas surrounding the alveolar ducts and terminal bronchioles. Conclusions: On a mass dose basis, chrysotile that have 1,700 times numbers of fibers per unit weight than anthophyllite produced a greater persistent lung injury than anthophllite for at least 4 weeks after exposure.
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에너지 분산 X선 분석장치가 장착된 투과전자현미경을 이용한 석면분석방법
한정희,김광진,정용현,이준연,이용묵,정호근,유일재 한국산업위생학회 2001 한국산업보건학회지 Vol.11 No.2
To establish and accurate asbestos analysis method for workplace samples, chrysotile, amosite, crocidolite, tremolite, actinolite, and anthophyllite asbestos fibers were analyzed for their morphology, atomic content and electron diffraction patterns. The morphology of asbestos fiber was evaluated in 10,000 ×magnification. The atomic contents was analyzed by a transmission electron microscopy with energy dispersive X-ray analyzer (TEM-EDX). Asbestos fivers were further assessed using electron diffraction(ED) patterns to provide an addtional criterion for classifying the asbestos fibers. Twenty asbestos fibers were initially randomly selected for morphological evaluation, based on an aspect ratio(length : diameter = 3:1). Then the fibers were determined for their EDX spectrums and ED patterns. Our results showed that only chrysotile fiber has a hollow tube structure to be distinguished from other asbestos fibers. Although asbestos fibers had similar morphology, they had different EDX spectrums and ED patterns. Our results on the atomic content of asbestos fibers were very similar to those of other researchers, but amosite and crocidolite had a little difference in atomic content compared with the results from other researcher. The difference may be due to the difference in equipment or asbestos sample selection. A study on asbestos samples from biological specimens to establish a criterion for determining occupational asbestos exposed diseases should be done in the near future.
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