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- 주제어 : alveolar bone marrow stem cells (검색결과 5 건)
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이응태 ( Eung Tae Lee ),임기택 ( Ki Taek Lim ),김장호 ( Jang Ho Kim ),임애리 ( Ae Li Im ),손현목 ( Hyun Mok Son ),조종수 ( Chong Su Cho ),정필훈 ( Pill Hoon Choung ),정종훈 ( Jong Hoon Chung ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4
Low-intensity ultrasound stimulation produces significant multi-functional effects that are directly relevant to bone formation. It was previously found that low-intensity ultrasound stimulation enhanced bone regeneration although the exact cellular mechanism is not clear. The aim of the present study is to investigate the effect of low-intensity ultrasound stimulation on proliferation of alveolar bone marrow stem cells. Before low-intensity ultrasound stimulation, alveolar bone marrow stem cells were cultured for 24h to facilitate their attachment. The cells were cultured in medium with or without low-intensity ultrasound stimulation. The ultrasound frequency was 1 MHz. Cell cultures stimulated with ultrasound were conducted by three treatment groups - group 1: intensity(100, 200, 300, 400 and 500 mW/cm2), group 2: duty cycle(5, 10, 30 and 50%) and group 3: duration time(1, 3, 5, 10, 20 and 30 min). The effect of low-intensity ultrasound stimulation were evaluated by cell number and morphological changes. The proliferation rates of alveolar bone marrow stem cells for the particular stimulated groups were larger than those of control groups. After low-intensity ultrasound stimulation(intensity: 100 mW/cm2, duty cycle: 30% and duration time: 10 min), the alveolar bone marrow stem cell counts were significantly increased(p < 0.05). This study suggested that the cell growth could be enhanced by appropriate low-intensity ultrasound stimulation.
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( Beom Su Kim ),( Hark Mo Sung ),( Hyung Keun You ),( Su Jin Kim ),( In Seop Kim ),( Jun Lee ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.2
Mesenchymal stem cells (MSCs) and scaffolds may overcome the limitations of traditional bone grafts. We investigated growth and osteoblast differentiation of human alveolar bone marrow-derived MSCs (hABMMSCs) in fibrin gel (FG) 3-dimensional (3D) culture. We compared hABMMSCs with human iliac crestderived MSCs (hICMSCs) and alginate gel (AG) was compared with FG. Morphological, histological, cell proliferation and viability characteristics of 3D cultured hABMMSCs were defined and compared with hICMSCs and AG. Gel-embedded cells were cultured in medium containing osteogenic differentiation stimulant (OS) and differentiation was assessed by alkaline phosphate (ALP) activity and mRNA expression of osteoblast marker genes. Isolated hABMMSCs and hICMSCs exhibited fibroblast-like morphology and expressed CD44, CD73, CD, 90, CD105, and CD106, but not CD34. hABMMSC proliferation in 3D FG culture was about 3-fold greater than that in 3D AG culture. hABMMSCs and hICMSCs exhibited a rounded morphology in AG, and fibroblastic morphology in FG. OS treatment increased ALP activity in both hABMMSCs and hICMSCs cultured in FG but yielded no significant changes in AG. Activity reached a maximum on culture day 10, and decreased on day 15. Following OS treatment, mRNA expression of ALP and osteopontin (OPN) increased in hABMMSCs and hICMSCs in FG by 3-fold over that in AG. mRNA expression of collagen type Iα1 (ColIα1) and osterix (OSX) were also 2-fold and 6-fold greater in FG than in AG.
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( Ki Taek Lim ),( Chong Su Cho ),( Yun Hoon Choung ),( Hoon Seon Woo ),( Hyun Mok Son ),( Soo Jung Baik ),( Jang Ho Kim ),( Sung Min Hong ),( Joo Young Park ),( Soung Hoo Jeon ),( Pill Hoon Choung ),( 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.14
We have developed and evaluated a novel perfusion flow bioreactor system which facilitates the observation of living cells for extended periods of time. Cell viability showed that cell growth rate cultured (without medium perfusion) using the novel flow perfusion chamber system revealed a similar trend (p>0.05) compared to that of static culture. Also, the cell viability under the static culture (without perfusion) and flow perfusion culture (flow rate: 0.03 ml/min) with the developed perfusion bioreactor system was compared. The cell growth rate of the flow perfusion culture was significantly higher (p<0.05) than that of static culture for 4 days. The cell growth rate was efficiently increased to about 10-15% with the precision perfusion culture. These results indicate that a bioreactor system that can provide mechanical signals has important applications. Additionally, the developed cell culture chamber system has been successfully used to maintain and record the morphology of cultured alveolar bone marrow stem cells. It was possible to monitor and capture the live cell morphology cultured in the perfusion bioreactor. The characteristics of the bioreactor developed in this study was fast to culture stem cells with mechanical stimulation and to monitor live cell imaging while promoting healthy cellular activity outside of an incubator environment. Through experimental results, the developed perfusion bioreactor could increase cell growth with proper flow based on mechanical stimuli.
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중간엽 줄기세포에서 후코이단에 의한 골 형성 분화 유도에 대한 영향
김범수,강효진,박지윤,양선식,윤정훈,이준 대한구강악안면병리학회 2014 대한구강악안면병리학회지 Vol.38 No.3
Fucoidan has been extensively studied as medicinal materials due to its biological activities including osteoblastic differentiationeffect. However, osteoblastic effect by fucoidan is unknown in alveolar bone marrow derived mesenchymalstem cells (ABM-MSCs). The present study was undertaken to evaluate the effect of fucoidan on Osteoblastic differentiationin ABM-MSCs and explore its mechanism. Cell proliferation was analyzed by crystal violet staining. Osteoblast differentiationwas determined by alkaline phosphatase activity, calcium accumulation assay and gene expression of osteoblast markers. We found that fucoidan induced cell proliferation of ABM-MSCs. Furthermore, fucoidan increased the ALP activity, calciumaccumulation, and osteoblast specific genes such as Runx2, type I collagen alpha 1. Moreover, fucoidan induces the expressionof asporin and bone morphogenic protein (BMP)-2 and asporin. Based on these results, these finding indicatethat fucoidan induces osteoblast differentiation in ABM-MSCs and partially enhanced the mRNA expression of BMP-2 andasporin.
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중간엽 줄기세포에서 후코이단에 의한 골 형성 분화 유도에 대한 영향
김범수,강효진,박지윤,양선식,윤정훈,이준 대한구강악안면병리학회 2014 대한구강악안면병리학회지 Vol.38 No.3
Fucoidan has been extensively studied as medicinal materials due to its biological activities including osteoblastic differentiation effect. However, osteoblastic effect by fucoidan is unknown in alveolar bone marrow derived mesenchymal stem cells (ABM-MSCs). The present study was undertaken to evaluate the effect of fucoidan on Osteoblastic differentiation in ABM-MSCs and explore its mechanism. Cell proliferation was analyzed by crystal violet staining. Osteoblast differentiation was determined by alkaline phosphatase activity, calcium accumulation assay and gene expression of osteoblast markers. We found that fucoidan induced cell proliferation of ABM-MSCs. Furthermore, fucoidan increased the ALP activity, calcium accumulation, and osteoblast specific genes such as Runx2, type I collagen alpha 1. Moreover, fucoidan induces the expression of asporin and bone morphogenic protein (BMP)-2 and asporin. Based on these results, these finding indicate that fucoidan induces osteoblast differentiation in ABM-MSCs and partially enhanced the mRNA expression of BMP-2 and asporin.
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