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Traction force microscopy for understanding cellular mechanotransduction
( Sung Sik Hur ),( Ji Hoon Jeong ),( Myung Jin Ban ),( Jae Hong Park ),( Jeong Kyo Yoon ),( Yongsung Hwang ) 생화학분자생물학회 2020 BMB Reports Vol.53 No.2
Under physiological and pathological conditions, mechanical forces generated from cells themselves or transmitted from extracellular matrix (ECM) through focal adhesions (FAs) and adherens junctions (AJs) are known to play a significant role in regulating various cell behaviors. Substantial progresses have been made in the field of mechanobiology towards novel methods to understand how cells are able to sense and adapt to these mechanical forces over the years. To address these issues, this review will discuss recent advancements of traction force microscopy (TFM), intracellular force microscopy (IFM), and monolayer stress microscopy (MSM) to measure multiple aspects of cellular forces exerted by cells at cell-ECM and cell-cell junctional intracellular interfaces. We will also highlight how these methods can elucidate the roles of mechanical forces at interfaces of cell-cell/cell-ECM in regulating various cellular functions. [BMB Reports 2020; 53(2): 74-81]
The role of cellular traction forces in deciphering nuclear mechanics
Rakesh Joshi,한성범,조원기,Dong Hwee Kim 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4
Cellular forces exerted on the extracellular matrix (ECM) during adhesion and migration under physiological and pathological conditions regulate not only the overall cell orphology but also nuclear deformation. Nuclear deformation can alter gene expression, integrity of the nuclear envelope, nucleus-cytoskeletal connection, chromatin rchitecture, and, in some cases, DNA damage responses. Although nuclear deformation is caused by the transfer of forces from the ECM to the nucleus, the role of intracellular rganelles in force transfer remains unclear and a challenging area of study. To elucidate nuclear mechanics, various factors such as appropriate biomaterial properties, rocessing route, cellular force measurement technique, and micromanipulation of nuclear forces must be understood. In the initial phase of this review, we focused on various engineered iomaterials (natural and synthetic extracellular matrices) and their manufacturing routes along with the properties required to mimic the tumor microenvironment. Furthermore, e discussed the principle of tools used to measure the cellular traction force generated during cell adhesion and migration, followed by recently developed techniques to auge nuclear mechanics. In the last phase of this review, we outlined the principle of traction force microscopy (TFM), challenges in the remodeling of traction forces, microbead isplacement tracking algorithm, data transformation from bead movement, and extension of 2-dimensional TFM to multiscale TFM.
Recent Advances in Biological Uses of Traction Force Microscopy
조영빈,박은영,고은민,박진성,신현정 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.17 No.10
Cell traction forces (CTF) generated by the actomyosin cytoskeleton onto a substrate or extracellular matrix (ECM) are essential for many biological processes, including developmental morphogenesis, tissue homeostasis, and cancer metastasis. Because the cellular physical properties are closely related to the pathological states of the cells, affected by various physicochemical stimuli from their neighboring cells or surrounding environments, it is crucial to develop a quantitative measure for cellular responses to these external stimuli. Since the pioneering work of Harris et al. in 1980s1, traction force microscopy (TFM) has been widely used as a standard tool that allows the optical measurement of cellular tractions exerted on 2- and 3-dimensional soft elastic substrates. Recently, there have been many technical advances in conventional TFM to enhance its spatial and temporal resolutions as well as the range of applicability. In this review, we provide a survey on the recent advancement in TFM, especially with a special emphasis on platforms that can externally apply various stimuli such as fluid shear, mechanical tension or compression, biochemical factors, and electric field in a physiologically relevant regime.
암 전이 과정에서 세포 분산 시 미세관의 메커니즘 규명
이성우(S. W. Lee),민찬홍(C. Min),조영빈(Y. Cho),신현정(Jennifer H. Shin) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.11월
암의 전이는 암세포가 처음 형성된 곳을 떠나 근처 림프절 또는 혈관 벽을 통해 이동해 다른 조직에 2 차 종양을 형성하는 현상을 말한다. 이 과정에서 종양 조직으로부터 암세포들이 군집 또는 개별적으로 떨어져 나와 이동하는 세포 분산 현상이 전이를 개시한다. 암의 전이를 억제하는 전략으로써 세포의 증식 능력 및 운동성을 저하시켜 세포 분산을 막기 위해 미세관 억제제 사용이 제안되어 활용되었다. 특히, 암세포 내의 미세관은 미세환경에 존재하는 근접한 여러 다른 세포들과 세포 외 기질 그리고 그 안에 존재하는 생화학 및 기계적 자극에 민감하게 반응하여 세포의 운동성을 조절하는 핵심요소로 알려져 있다. 따라서 미세관의 구조적 및 기능적 특성이 세포 분산에 미치는 영향을 이해하는 것은 암 전이의 메커니즘을 근본적으로 파악하는데 필수적이다. 이에 본 연구에서는 암의 기계적 및 화학적 환경을 모사하는 하이드로젤을 제작하고 그 위에 유선상피세포주(MCF10A)로 이루어진 원형 마이크로 패턴을 부착해 세포 분산 모델을 구축하였다. 이를 활용해 세포의 견인력을 측정하는 TFM (Traction Force Microscopy)을 수행해 세포가 바닥면에 가하는 힘과 군집의 이동 특성 사이에 상관관계가 존재하고 미세관의 억제 유무에 따라 두 물리적 거동이 뚜렷하게 달라짐을 밝혀내었다. 본 연구는 암 군집의 세포 분산 과정에서 미세관의 역할을 물리적 관점에서 고찰하는 새로운 해석을 제시하고자 한다.
PIV 및 TFM 측정 기법을 이용한 예쁜꼬마선충의 동적 패턴 가시화 연구
박진성(Jin-Sung Park),윤병환(Byoung Hwan Yun),신현정(Jennifer H. Shin) 한국가시화정보학회 2014 한국가시화정보학회지 Vol.12 No.2
Caenorhabditis elegans (C. elegans) is an undulatory nematode which exhibits two distinct locomotion types of swimming and crawling. Although in its natural habitat C. elegans lives in a non-Newtonian fluidic environment, our current understanding has been limited to the behavior of C. elegans in a simple Newtonian fluid. Here, we present some experimental results on the penetrating behavior of C. elegans at the interface from liquid to solid environment. Once C. elegans, which otherwise swims freely in a liquid, makes a contact to the solid gel boundary, it begins to penetrate vertically to the surface by changing its stroke motion characterized by a stiffer body shape and a slow stroke frequency. The particle image velocimetry (PIV) analysis reveals the flow streamlines produced by the stroke of worm. For the worm that crawls on a solid surface, we utilize a technique of traction force microscopy (TFM) to find that the crawling nematode forms localized force islands along the body where makes direct contacts to the gel surface.