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( Chang Sun Kim ),( Ji Yeon Kim ),( Hyo Jin Kim ) 한국운동영양학회 2014 Physical Activity and Nutrition (Phys Act Nutr) Vol.18 No.1
[Purpose] The purpose of this study was to examine the effect of a single bout pilates exercise on mRNA expression of bone metabolic cytokines in elderly osteopenia women. [Methods] We selected 11 people of elderly osteopenia women and loaded a single bout pilates exercise about RPE 10-14 level. The blood samples were collected before, immediately after and 60 minute after pilates exercise, then examined calcium metabolic markers in serum and extracted peripheral blood mononuclear cell (PBMC) from whole blood and confirmed mRNA expression of bone metabolic cytokines from PBMC. To clarify the changes during exercise, we designed repeated measure ANOVA as the control group to perform blood sampling without exercise. [Results] As a result, serum P showed significant interaction effect between group and time (p<.001), the pilates exercise group decreased about 9% at immediately after exercise and 13% during recovery after exercise (p<.05), while the control group showed a tendency to increase. Serum CK also showed a significant interaction between group and time (p<.05), the pilates group significantly increased at immediately after exercise and during recovery after exercise (p<.05) but the control group didn`t have changes. TNF-α and IL-6 mRNA expression in PBMC was significantly increased in the pilates group (p<.01, p<.05), although INF-γ mRNA expression didn`t show statistically significant difference, it tended to increase in the pilates group (NS). [Conclusion] These results suggested that a single bout pilates exercise of elderly osteopenia women cause hypophosphatemia with temporary muscle damage, and it leading high turnover bone metabolic state with to activate both of bone formation and bone resorption.
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명아주 에탄올 추출물의 TNF-α, IL-1α 생성 억제와 TNF-α, IL-1α mRNA 발현 억제 효과
박해련 ( Hye-ryeon Park ),김기영 ( Ki-young Kim ) 한국미용학회 2017 한국미용학회지 Vol.23 No.5
In this study, anti-oxidant and anti-inflammatory effect of Chenopodium album var. centrorubrum (C. album) were investigated in order to observe the usability as a natural cosmetics material. C. album ethanol extract was tested for i) free radical scavenging activity, ii) production inhibition of TNF-a and IL-1a in RAW 264.7 cell, and iii) inhibition of TNF-α and IL-1α mRNA expression in HaCaT cell. C. album ethanol extract resulted scavenging effect of DPPH(39.7 ± 2.1%) and SO(33.7 ± 2.3%) at 10 ppm. Production inhibition of TNF-α and IL- 1α was significantly higher than the LPS-untreated cells, but significantly lower than only LPS-treated cells(p<0.001). mRNA expression of TNF-α(35.2 ± 2.2%) and IL-1α(2.8 ± 0.3%) was significantly lower than positive control d-panthenol(52.6 ± 3.1%, 55.9 ± 2.0%) in HaCaT cells. In conclusion, C. album may be applicable as a new natural materials for aging skin, oily skin, and acne skin.
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Jo, Eun-Kyeong,Park, Jeong-Kyu,Kim, Hwa-Jung,Pyun, Kwang-Ho,Paik, Tae-Hyun 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-
Mycobacterial antigens can be divided into several groups on the basis of their subcellular Iocalization (cytoplasmic, cell wall associated, or secreated). Compared to the secreted protein antigen, little is known about the surface protein antigens associated with the cell wall of Mycobacterium tuberculosis. This is the first study on the isolation and the cellualr immune responses of Triton X-100 solubilized protein (TSP) antigen which may be preferentially associated with the cell wall of M. tuberculosis. We investigated the immunological responses of peripheral blood mononuclear cells (PBMCs) to the TSP antigen in tuberculosis (TB) patients and healthy individuals, and compared with those induced by the 30 kDa antigen from M. tuberculosis H37Rv culture filtrates, identified as a biologically important secreted protein. Significant lymphoproliferative responses to the TSP antigen were observed in healthy tuberculin reactive donors, newly diagnosed TB patients and some of the chronic TB patients after a 6-day in vitro stimulation; these being higher than those to the 30 kDa antigen. Furthermore, analysis by flow cytometry indicated an preferential expansion of CD^4+ T lymphocytes by the TSP antigen in healthy tuberculin reactive donors and patients with pulmonary TB. Treatment of PBMCs of healthy tuberculin reactors with TSP antigen for longer than 48 hrs resulted in progressive augmentation of IFN-γ, IL-2 and TNF-α mRNA measured by RT-PCR, but considerably reduced IL-4 mRNA expression after 5 days. After stimulation with the 30 kDa antigen, the rates of IFN-γ mRNA expression without detectable IL-4 mRNA in healthy controls and TB patients were 13.0% and 4.2%, respectively. Interestingly, those by the TSP antigen were increased to 34.8% and 45.8%. Furthermore, two other immune manifestations to the TSP antigen were observed in the TB patient of treatment-failed cases. The high responders showed a 10-fold-higher lymphoproliferation and induced more IFN-γ, IL-2 and TNF-α mRNA expression upon the TSP antigen stimulation than the low responders. After 2 months, they converted to negative on a direct smear and/or culture, and clinically and radiographically improved. These results suggest that the TSP antigen of M. tuberculosis may be highly immunogenic, a strong inducer of cytokine mRNA associated with Th1 and/or macrophage activation, such as IFN-γ, IL-2 and TNF-α. IN conclusion, the TSP antigen can be used as a T cell immunogen associated with protective immunity against tuberculosis in humans.
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한국산 겨우살이 추출물 M11C(렉틴 구성물질)가단구세포의 TNF-α 유전자 발현유도 및 분비에 비치는 효과
허억 한국약용작물학회 2007 韓國藥用作物學會誌 Vol.15 No.1
It is well-known that Korean mistletoe (Viscum album) extract has an immune activity and anticancer effect. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to examine whether this extract might activate human peripheral monocyte to produce tumor necrosis factor-α (TNF-α). To examine the effect of M11C on the production of TNF-α from monocyte, the monocyte were stimulated by the M11C, and then collected the supernatant (M11C stimulated monocyte-conditioned media; MCM). MCM was treated to the TNF-α sensitive L929 cells, and then L929 cytotoxicity was measured by means of MTT. MCM had cytotoxic effect on L929. And the cytotoxic effect of MCM on L929 was almost abolished by anti-TNF-α antibody. These data indicated that MCM contained TNF-α, suggesting the TNF-α generation from M11C-stimulated monocyte. This suggestion was confirmed from the data that TNF-α was highly detected in MCM by immunoblotting technique. M11C effect on TNF-α production from monocyte was in the dose and stimulating time dependent manners. Also the effect of M11C on the expression of TNF-α mRNA from monocyte was shown in the dose and stimulating time dependent manners. As a result, Korean mistletoe extract, M11C, could be used for an immunostimulator.
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