영산강 동계 조류 대발생 기간의 규조류 Stephanodiscus spp. 출현양상과 형태적 분류

정병관,김용재,정승원,이학영,신용식 한국하천호수학회 2014 생태와 환경 Vol.47 No.4

영산강에서 동계 대증식을 유발하는 Stephanodiscus속의 출현기간과 종 분류를 위해 2012년 12월부터 2013 년 4월까지 주 단위로 21회의 모니터링을 실시하였다. 동계 영산강에서는 규조류가 절대적으로 우세한 군집을 보였으며, 규조강 중 원형규조 Aulacoseira, Cyclotella, Stephanodiscus속이 주요 출현종으로 동정되었다. 이 중 Stephanodiscus spp.는 극우점 규조류로서 12월 초에 출현하기 시작하여 1월에 최대 개체수를 나타냈고, 3월까지 높은 분포를 보였다. 기온이 증가하기 시작하는 4월부터 개체수가 급격히 감소한 반면 녹조강, 남조강, 은편모조강 등이 증가하는 경향을 나타냈다. 우점종은 거의 모든 조사시기에 원형 중심목 규조류 (S. hantzschii)가 우점 및 차우점종으로 동정되었으며, 3월 말과 4월 중에는 남조강인 Anabaena sp.와 은편모조강인 Cryptomonas sp.가 우점 및 차우점종으로 출현함으로써 계절 변화에 따른 종 천이가 관찰되었다. 조사시기에 출현한 원형 중심목 규조류의 명확한 종 분류를 위해 전자현미경을 통해 동정한 결과, Stephanodiscus속 내의 S. hantzschii가 극우점한 가운데 부분적으로 S. minutulus와 S. parvus 동정되었으며, 그밖에 원형 중심목 규조류에는 Cyclostephanos속의 C. invisitatus와 Cyclotella속의 C. meneghiniana, Discostella속의 D. pseudostelligera, D. woltereckii가 동정되었다. We conducted the weekly monitoring (December 2012~April 2013) to evaluate the temporal variation and identification of Stephanodiscus spp. that are generally dominant during winter in the Yeongsan River. Phytoplankton species were identified and counted using the optical microscope and scanning electron microscope (SEM). Phytoplankton in the river were grouped into 6 classes (bacillariophyceae, chlorophyceae, cryptophyceae, cyanophyceae, dinophyceae, euglenophyceae), 30 genus and 41 species. Phytoplankton composition showed high abundance of diatoms in winter and Aulacoseira sp., Cyclotella sp. and Stephanodiscus spp. were dominant. Among the species, Stephanodiscus spp. was relatively abundant compared to other diatom species. Stephanodiscus spp. appeared from December 2012 to April 2013 and their abundance peaked in January. Abundance of diatoms especially peaked (21,080cells mL-1) in January 15, 2013 when Stephanodiscus spp. also bloomed (20,560cells mL-1). The abundances of Stephanodiscus spp. were gradually decreased from March and reached as low as 60cells mL-1 in April 26. Cyclostephanos (C. invisitatus), Cyclotella (C. meneghiniana), Discostella (D. pseudostelligera, D. woltereckii) and Stephanodiscus (S. hantzschii, S. minutulus, S. parvus) were classified in the circular diatoms. Abundance of S. hantzschii was extremely high compared to S. minutulus and S. parvus.

낙동강 하류부에서 Stephanodiscus속에 의한 수화 발생시 미생물먹이망 군집 동태

서정관 ( Jung Kwan Seo ),이혜진 ( Hae Jin Lee ),정익교 ( Ik Kyo Chung ) 한국환경생물학회 2010 환경생물 : 환경생물학회지 Vol.28 No.3

The bloom of the genus Stephanodiscus was gradually extinguished after 18 April. Counts of bacterial population were increased as the diatom bloom was disappeared. Numbers of the heterotrophic nanoflagellates and ciliates were also increased during the disappearance of the bloom. The densities of the mesozooplankton, the major predator of the diatoms, started to increase in April. However, their growth was suppressed during the bloom period of the diatoms (from January to March). During the bloom period of the diatoms, the monthly average value of the basic productivity amounted up to 11,765.7 mgC m-2 day-1, which is relatively high value considering the low temperature and light during that period. The growth rate of phytoplankton in March, when the bloom was beginning to be supressed was 0.007. The growth rate of phytoplankton was negative value in April when the decreasing of the algal density was started.

영산강수계 죽산보의 식물플랑크톤과 이화학적 변화

손미선,정현수,박창희,박종환,임채홍,김경현 한국환경생물학회 2018 환경생물 : 환경생물학회지 Vol.36 No.4

The objective of this study was to determine the changes in phytoplankton and longterm water quality of Juksan-Weir in Yeongsan River that took place between April 2010 and December 2015. The number of species used in this study was 288, which consisted of 6% of Cyanophyta, 26% of Bacillariophyta, 53% of Chlorophyta and the others (15%). The standing crops of phytoplankton ranged from 500 cells · mL-1 − 29,950 cells · mL-1 with an average of 7,885 cells · mL-1. At the two site, 20 dominant genera of found. The dominant genera were 6 of Bacillariophyta, 6 of Cyanophyta, 7 of Chlorophyta and 1 of Cryptophyta. The most dominant genus among the phytoplankton was Stephanodiscus sp. (Total 59%, each 54% and 63%). The most dominant genus among the Cyanophyta was Microcystis sp., which had a cell abundance ratio of 17%. The results of two sites were 21% and 13%, and the upstream was higher than the downstream. 2010년 4월부터 2015년 12월까지 영산강 본류에 위치해 있는 죽산보의 상, 하류의 수질 및 식물플랑크톤 변화 양상을 파악하기 위해 연구를 수행하였다. 동일한 기간 동안에 두지점 모두 평균 수온은 17.3°C로 기록되었으며, 2011년부터 2014년까지는 약 16.3°C를 유지하다가 2015년에 17°C로 약 0.7°C 상승되었다. 조사기간 동안 식물플랑크톤의 종 조성은 총 288 분류군으로 남조류 17 분류군, 규조류 74 분류군, 녹조류 154 분류군 및 기타조류 15 분류군으로 조사되었다. 두 지점의 개체군 밀도는 500~29,950 cells·mL-1으로 조사되었으며, Y1지점은 850~29,725 cells·mL-1, Y2지점은 500~29,950 cells·mL-1으로 조사되었다. 두 지점의 평균 개체군 밀도는 Y1지점이 8,180 cells·mL-1, Y2지점이 7,530 cells·mL-1으로 상류지점의 평균 밀도가 더 높게 조사되었다. 우점종은 규조류인 Stephanodiscus속, Aulacoseira속이 높게 나타났으며, Stephanodiscus의 우점 빈도는 59%였고, Aulaocseira속의 우점빈도는 22%였다. 남조류의 전체속의 우점빈도는 17%였고, Y1지점이 19%로 Y2지점보다 2% 높게 나타났다. 그 중에 Microcystis속의 우점 빈도가 6%였고, Y1지점이 7%로 하류인 Y2지점보다 2% 높게 나타난 것을 확인할 수 있었다. Y1지점의 현존량 비율은 규조류가 전체의 52%, 녹조류가 24%, 남조류가 21%였고, Y2지점은 규조류 65%, 녹조류 18%, 남조류 13%로 상류 지점인 Y1지점의 남조류 현존량 비율이 더 높게 나타난 것을 볼 수 있었다. 본 연구는 식물플랑크톤의 천이 현상 및 수질요인들과의 변화에 따른 원인을 파악하고 영산강 죽산보의 특성에 대해 신뢰성 있는 데이터를 제공하기 위해 수행되었으며, 이후 수생태계 변화를 평가하는 기초자료로 활용될 수 있을 것이다.

생물유래 살조물질 Naphthoquinone 유도체의 규조 Stephanodiscus 제어 효과 및 생태계 변화 모니터링: A case study

주재형(Jae-Hyoung Joo),박범수(Bum Soo Park),김세희(Sae Hee Kim),한명수(Myung-Soo Han) 응용생태공학회 2020 Ecology and resilient infrastructure Vol.7 No.1

저온수기 규조 Stephanodiscus sp.에 의한 녹조 현상은 빈번하게 일어나며, 이로 인한 수자원의 질적 변화와 정수과정에 많은 경제적 손실을 주고 있다. 선행연구를 통해 Stephanodiscus sp. 종의 친환경적 제어를 위해 개발된 생물유래 물질인 naphthoquinone (NQ) 유도체의 현장조건에서 살조효과 입증을 위해 서낙동강 수변에 5 ton 규모 mesocosm을 설치하여 현장조건에서의 살조효과와 비생물학적, 생물학적 요인을 모니터링 하였다. NQ 4-6 물질을 처리한 결과, 대상 조류인 Stephanodiscus sp.의 세포밀도가 실험 초기 5 × 10³ cells mL¹에서 급격하게 감소하여 실험 종료시인 10일차에는 0.2 × 10³ cells mL¹으로 세포 대부분이 사멸되어, 81%의 높은 살조효과가 관찰되었다. 또한, NQ 4-6 물질 접종 후 식물플랑크톤을 제외한 물리・화학적요인 (수온, 용존 산소, pH, 전기전도도, 영양염)과 생물요인 (박테리아, HNFs, 섬모충, 동물플랑크톤)에 영향을 미치지 않았으며, 대조구와 처리구에서 유사한 경향이 관찰되었다. 즉, NQ 4-6 물질의 현장 적용은 규조 Stephanodiscus sp. 종의 선택적 제어뿐만 아니라, 수생 생물의 서식처와 먹이 공급을 원활하게 함으로써 건강한 수 생태계를 형성하는데 큰 역할을 할 것으로 판단된다. Blooms of the small centric diatom Stephanodiscus is quite occasional in winter season in temperate freshwater ecosystems. Often, it leads to degradation of water quality and affects quality of supplied drinking water. In previous studies, naphthoquinone (NQ) compounds have been shown to be effective and selective for controlling winter bloom species Stephanodiscus hantzschii. We conducted a 5 ton scale mesocosm experiment to investigate the effects of NQ on native Stephanodiscus sp. collected from Nakdonggang River in water. After treatment with NQ 4-6 compound (0.2 μM), the cell density of Stephanodiscus sp. was rapidly reduced from 5 × 10³ cells mL<SUP>-1</SUP> to 0.2 × 10³ cells mL<SUP>-1</SUP> for 10 days. Additionally, NQ 4-6 compound did not affect physicochemical factors (water temperature, dissolved oxygen, pH, conductivity, nutrients) and biological factors (bacteria, heterotrophic nanoflagellates, zooplankton). Therefore, these findings suggest that the NQ 4-6 compound has potential as an alternative algicidal substances to effectively mitigate natural Stephanodiscus sp. blooms, and the application of NQ 4-6 compound will restore the healthy aquatic ecosystems.

영산강 동계 조류 대발생 기간의 규조류 Stephanodiscus spp. 출현양상과 형태적 분류

정병관 ( Byung Kwan Jeong ),김용재 ( Yong Jae Kim ),정승원 ( Seung Won Jung ),이학영 ( Hak Young Lee ),신용식 ( Yong Sik Shin ) 한국하천호수학회 2014 생태와 환경 Vol.47 No.4

We conducted the weekly monitoring (December 2012~April 2013) to evaluate the temporal variation and identification of Stephanodiscus spp. that are generally dominant during winter in the Yeongsan River. Phytoplankton species were identified and counted using the optical microscope and scanning electron microscope (SEM). Phytoplankton in the river were grouped into 6 classes (bacillariophyceae, chlorophyceae, cryptophyceae, cyanophyceae, dinophyceae, euglenophyceae), 30 genus and 41 species. Phytoplankton composition showed high abundance of diatoms in winter and Aulacoseira sp., Cyclotella sp. and Stephanodiscus spp. were dominant. Among the species, Stephanodiscus spp. was relatively abundant compared to other diatom species. Stephanodiscus spp. appeared from December 2012 to April 2013 and their abundance peaked in January. Abundance of diatoms especially peaked (21,080 cells mL-1) in January 15, 2013 when Stephanodiscus spp. also bloomed (20,560 cells mL-1). The abundances of Stephanodiscus spp. were gradually decreased from March and reached as low as 60 cells mL-1 in April 26. Cyclostephanos (C. invisitatus), Cyclotella (C. meneghiniana), Discostella (D. pseudostelligera, D. woltereckii) and Stephanodiscus (S. hantzschii, S. minutulus, S. parvus) were classified in the circular diatoms. Abundance of S. hantzschii was extremely high compared to S. minutulus and S. parvus.

The Effect of Water Temperature on Proliferation of Stephanodiscus sp. in vitro from the Nakdong River, South Korea

김명철,La Geung-Hwan,Kim Hyun-Woo,정광석,김동균,주기재 한국하천호수학회 2008 생태와 환경 Vol.41 No.1

To understand the effect of water temperature on growth pattern of Stephanodiscus sp., we weekly or biweekly investigated in the lower part of the Nakdong River from 1994 to 2006 and performed a laboratory experiment. Stephanodiscus was the most dominant species among phytoplankton in winter when low flow persisted and the high abundances of the species were maintained from December to February. Three strains of Stephanodiscus sp. were isolated for the in vitro experiment from the Nakdong River in January 2005. Over the water temperature range of 4'C to 20'C, the growth patterns of Stephanodiscus sp. were different in the short-term batch culture. The maximum cell density of Stephanodiscus sp. was observed at approximately 5C in the river systems, but the optimum water temperature of Stephanodiscus sp. was 10C for the growth in the laboratory experiment. However, the proliferation of Stephanodiscus sp. was related to low water temperature in the Nakdong River.

The Effect of Water Temperature on Proliferation of Stephanodiscus sp. in vitro from the Nakdong River, South Korea

Kim, Myoung-Chul,La, Geung-Hwan,Kim, Hyun-Woo,Jeong, Kwang-Seuk,Kim, Dong-Kyun,Joo, Gea-Jae The Korean Society of Limnology 2008 생태와 환경 Vol.38 No.S

To understand the effect of water temperature on growth pattern of Stephanodiscus sp., we weekly or biweekly investigated in the lower part of the Nakdong River from 1994 to 2006 and performed a laboratory experiment. Stephanodiscus was the most dominant species among phytoplankton in winter when low flow persisted and the high abundances of the species were maintained from December to February. Three strains of Stephanodiscus sp. were isolated for the in vitro experiment from the Nakdong River in January 2005. Over the water temperature range of $4^{\circ}C$ to $20^{\circ}C$, the growth patterns of Stephanodiscus sp. were different in the short-term batch culture. The maximum cell density of Stephanodiscus sp. was observed at approximately $5^{\circ}C$ in the river systems, but the optimum water temperature of Stephanodiscus sp. was $10^{\circ}C$ for the growth in the laboratory experiment. However, the proliferation of Stephanodiscus sp. was related to low water temperature in the Nakdong River.

Comparative Molecular Analysis of Freshwater Centric Diatoms with Particular Emphasis on the Nuclear Ribosomal DNA of Stephanodiscus (Bacillariophyceae)

Ki, Jang-Seu The Korean Society of Phycology 2009 ALGAE Vol.24 No.3

DNA-based discrimination of species is a powerful way for morphologically otherwise similar species, like centric diatoms. Here, the author sequenced long-range nuclear ribosomal DNAs, spanning from the 18S to the D5 region of the 28S rDNA, of Stephanodiscus, particularly including a Korean isolate. By comparisons, high DNA similarities were detected from the rDNAs of nine Stephanodiscus (>99.4% in 18S rDNA, >98.0% in 28S rDNA). Their genetic distances, however, were significantly different (Kruskal-Wallis test, p < 0.01) compared to two related genera, namely Cyclotella and Discostella. In addition, genetic distances of 18S rDNAs were significantly different (Student’s t-test, p = 0.000) against those of the 28S rDNAs according to individual genera (Cyclotella, Discostella, and Stephanodiscus). Phylogenetic analyses showed that Stephanodiscus and Discostella showed a sister taxon relationship, and their clade was separated from a cluster of Cyclotella (1.00 PP, 100% BP). This suggests that Stephanodiscus has highly conserved sequences of both 18S and 28S rDNA; however, Stephanodiscus is well-separated from other freshwater centric diatoms, such as Cyclotella and Discostella, at the generic level.

Comparative Molecular Analysis of Freshwater Centric Diatoms with Particular Emphasis on the Nuclear Ribosomal DNA of Stephanodiscus (Bacillariophyceae)

기장서 한국조류학회I 2009 ALGAE Vol.24 No.3

DNA-based discrimination of species is a powerful way for morphologically otherwise similar species, like centric diatoms. Here, the author sequenced long-range nuclear ribosomal DNAs, spanning from the 18S to the D5 region of the 28S rDNA, of Stephanodiscus, particularly including a Korean isolate. By comparisons, high DNA similarities were detected from the rDNAs of nine Stephanodiscus (>99.4% in 18S rDNA, >98.0% in 28S rDNA). Their genetic distances, however, were significantly different (Kruskal-Wallis test, p < 0.01) compared to two related genera, namely Cyclotella and Discostella. In addition, genetic distances of 18S rDNAs were significantly different Student’s t-test, p = 0.000) against those of the 28S rDNAs according to individual genera (Cyclotella, Discostella, and Stephanodiscus). Phylogenetic analyses showed that Stephanodiscus and Discostella showed a sister taxon relationship, and their clade was separated from a cluster of Cyclotella (1.00 PP, 100% BP). This suggests that Stephanodiscus has highly conserved sequences of both 18S and 28S rDNA; however, Stephanodiscus is well-separated from other freshwater centric diatoms, such as Cyclotella and Discostella, at the generic level.

응집공정과 전산화처리를 이용한 규조류(Stephanodiscus) 및 남조류(Microcystis)의 제거에 관한 연구

임용승 ( Im Yong Seung ),김영진 ( Kim Yeong Jin ),이철우 ( Lee Cheol U ),강임석 ( Kang Im Seog ) 한국물환경학회 2003 한국물환경학회지 Vol.19 No.1

Algae in water source causes many problems not only to the quality of treated water, but also to the operation of drinking water treatment processes. Therefore, it is essential to remove algae throughout water treatment process, especially during coagulation and preoxidation process. At coagulation experiment of a different coagulant species for removal algae, Microcystis showed that the removal efficiency was similar to alum, PSO-M, PASS and PAC coagulant. The coagulation efficiency of Stephanodiscus and Microcystis after prechlorination and preozonation treatment was increased the one that than use of only alum coagulant. Therefore circumstances of ozone treatment was prefitable removal efficiency of dissolved organic matter(DOC) and was few production of 1㎛ particles than chlorine treatment. The result of Algogenic Organic Matter (AOM) classification for Stephanodiscus and Microcystis, hydrophilic, humic and fulvic acid component showed each 59%, 26%, 15% and 38%, 36%, 26% order. The result after two day that only Microcystis input in Milli-Q water, DOC released from showed 1.16㎎/L. DOC released from the Microcystis after chlorine and ozone treatment increased to about 15∼62%. Also, According to the rapid sand filter tests after preoxidation processes of Stephanodiscus and Microcystis, the preoxidation process using ozone or chlorine lead to reduce the rate of headloss development and prevent from breakthrough of turbidity than only alum treatment.