Rhodococcus sp. 을 이용한 생물처리 시스템에 의한 hexane 제거 특성

조경숙,이은희 한국냄새환경학회 2005 실내환경 및 냄새 학회지 Vol.4 No.2

In this study, we investigated the characteristics of hexane removal in biological processes such as bofilter and biotrickling filter inoculated with Rhodococcus sp. EH831 or Rhodococcus sp. EC1. Hexane was removed in the biofilter inoculated with Rhodococcus sp. EH831 after lag period of 3 days, and then hexane elimination capacity and removal efficiency increased to 30 g•m-3•h-1 and more than 70%, respectively. However, the biofilter inoculated with Rhodococcus sp. EC1 showed lower hexane removal efficiency of 20~30% during initial 10 days, and it was up to 50% with large fluctuation. In the biotrickling filter inoculated with strain EH831, average hexane removal efficiency was 50%. These results indicate that biofilter and biotrickling filter system couldn’t offer the optimal system for hexane removal, although the systems have been widely used for the removal of other volatile organic compounds such as benzene, toluene, xylene, and styrene. Therefore, a novel biological system should be developed for the treatment of waste gas containing hexane. 본 연구에서는 Rhodococcus sp. EH831과 EC1을 사용하여 생물처리 시스템인biofilter와 biotrickling filter의 hexane 제거 특성을 조사하였다. Rhocococcus sp. EH831이 접종된 biofilter는 3일의 적응기후 hexane을 분해하기 시작하였고, hexane 제거능은 30 g•m-3•h-1, 제거효율은 70% 이상으로 향상되었다. 그러나, Rhodococcus sp. EC1이 접종된 biofilter는 초기 10일 까지는 hexane 제거율이 약 20~30%로 비교적 낮은 값을 보였고, 그 후 제거율이 50% 까지 향상되었으나 변동이 크게 나타났다. Rhodococcus sp. strain EH831이 접종된 biotrickling filter는 평균적으로 50%의 제거율을 보였다. 이러한 결과는 비록 biofilter와 biotrickling filter가 benzene, toluene, xylene, 그리고 styrene과 같은 VOCs 처리에 효과적으로 사용되었음에도 불구하고 hexane 제거에는 최적 조건을 제공하지 못한다는 것을 의미한다. 그러므로 hexane 폐가스 처리를 위해서는 새로운 개념의 생물학적 처리공정의 개발이 필요하다.

Rhodococcus sp. EL-GT 에 의한 고농도 페놀의 분해 특성

박근태,차미선,남귀숙,조순자,손홍주,이건,이상준 한국환경과학회 2002 한국환경과학회지 Vol.11 No.9

본 연구는 자연계에서 분리 동정된 페놀분해능이 우수한 균주의 최적 페놀 분해 조건과 Biofilm 형성여부를 조사하는 목적으로 실험을 행하였다. 본 Rhodococcus sp. EL-GT는 저농도에서는 물론이고 10 mM 이상의 고농도 페놀의 분해가 가능하였으며 30 mM까지도 분해 및 생육이 이루어졌다. 질소원은 무기질 중 가장 높은 활성을 보인 NH_4NO_3와 yeast extract에서의 결과를 비교해보면 생육도는 차이가 많이 났으나 페놀의 분해능에서는 큰 차이를 나타내지 않아 경제성이 보다 나은 무기질소원인 NH_4NO_3을 질소원으로 선정하였다. 각종무기염에 대한 농도는 MgSO_4·7H_2O는 1 mM, CaCl_2·2H_2O의 경우 50 μM, FeCl_3는 0.5 μM을 최적농도로 결정하였다. 또한 Rhodococcus sp. EL-GT는 phenol외에도 다양한 방향족 화합물 및 염소계화합물을 기질로 이용하여 생육함을 알 수 있었다. 분해가 원활이 이루어지는 배양온도의 범위는 20∼40℃였으며, 본 공시균주의 경우 담체부착능도 우수한 것으로 나타났으므로 생물막법에 의한 폐수처리에도 적용하는 등 차후의 계속된 연구를 통해 산업현장에서의 응용 가능성이 매우 클 것으로 사료된다. The isolated strain, Rhodococcus sp. EL-GT was able to degrade high phenol concentrations up to 10 mM within 24 hours in the medium consisting of 5.3 mM KH_2PO_4, 95 mM Na_2HPO_4, 18mM NH_4NO_3, 1 mM MgSO_4 ·7H_2O, 50 μM CaCl_2, 0.5 μM FeCl_3, initial pH 8.0, temperature 30℃ in rotary shaker at 200 rpm. This strain was good cell growth and phenol degradation in the alkaline pH range range, and the highest in the pH range of 7 to 9. The microorganism was able to grow at the various chlorinated phenols, benzene, toluene, and bunker-C oil. As Rhodococcus sp. EL-GT was good capable of attachment on the acryl media, it would be used as microorganism to consist of biofilm in wastewater treatment.

Isolation, identification and characterization of marine bacteria exhibiting complementary enantioselective epoxide hydrolase activity for preparing chiral chlorinated styrene oxide derivatives

우정희,강경명,권태형,박년호,이은열 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.28 No.-

Marine bacteria possessing epoxide hydrolase (EH) activity were isolated from the oil-spilledforeshore of South Korea. While the isolated Rhodococcus sp. YSMI04 and YSNA32 showed differentenantioselectivities to racemic styrene oxide (SO), two strains exhibited same enantiopreference to3-chlorostyrene oxide (3-CSO) and 4-CSO. In the case of 3-CSO, Rhodococcus sp. YSMI04 and YSNA32exhibited (R)-3-CSO preferred hydrolysis activity, whereas Roseobacter sp. TSBP12 showed a preference for(S)-3-CSO. In the case of 4-CSO, Rhodococcus sp. YSMI04 and YSNA32 showed enantiopreference to(S)-4-CSO, Roseobacter sp. TSBP12 exhibited (R)-4-CSO preferred activity. (S)-4-CSO was obtainedwith 35% yield (theoretically 50%) using Roseobacter sp. TSBP12

신균주 Rhodococcus sp. YSPW01과 YSPW02를 이용한 수중 내 고농도 Perchlorate 환원 특성 연구

이상훈(Sang Hoon Lee),황재훈(Jae Hoon Hwang),Kabra Akhil,이대성(Dae Sung Lee),전병훈(Byong Hun Jeon) 大韓環境工學會 2014 대한환경공학회지 Vol.36 No.5

수중 내 함유된 고농도 perchlorate의 환원가능성을 perchlorate 환원 미생물인 신균주 Rhodococcus sp. YSPW01과YSPW02로 검토하였다. Perchlorate 환원 미생물은 혐기소화조 슬러지에서 분리 배양하였으며, perchlorate 환원능을 검토하기위해 전자공여체인 acetate를 사용하였다. YSPW01과 YSPW02는 perchlorate 82 mg L-1와 acetate 550 mg L-1에서 회분식 실험을 수행하였을 때, 반응 26시간과 9시간 후 각각 정상상태에 도달하였다. 이 때 perchlorate 환원은 8, 7시간 이내에 초기농도82 mg L-1에서 검출한계 이하(10 μg L-1)까지 제거되었다. 반응조 내에 acetate:perchlorate (w:w)비를 1:1에서 5:1로 증가한결과, perchlorate의 제거 속도는 YSPW01과 YSPW02 모두 2.1, 3.2 mg L-1 h-1에서 15, 15.5 mg L-1 h-1로 약 4.5배 증가하였고,최종 perchlorate는 검출한계 이하까지 제거되었다. 본 연구결과, Rhodococcus sp. YSPW01 및 YSPW02는 고농도 perchlorate제거에 적합하며, 신균주를 고농도 perchlorate 제거를 위한 생물학적 처리공정에 응용가능 할 것으로 판단된다. The feasibility of perchlorate reducing-bacteria isolated from the sludge of an anaerobic digester was determined usingammonium perchlorate in aqueous medium. Growth kinetics of the two perchlorate reducing bacteria including Rhodococcus sp. YSPW01 and YSPW02 were investigated using acetate as the electron donor in batch experiment. The growth of YSPW01 andYSPW02 reached a steady-state at 26 and 9 h, respectively. The initial perchlorate concentration was completely reduced within8 and 7 h by YSPW01 and YSPW02, respectively. The reduction rates were 2.1 and 15 mg L-1 h-1 for YSPW01, and 3.2 and 15.5mg L-1 h-1 for YSPW02, at 1:1 and 5:1 ratios of acetate:perchlorate (w:w), respectively. In this study, the bacteria Rhodococcussp. YSPW01 and YSPW02 demonstrated a potential for the perchlorate reduction, which could be further investigated for developmentof an efficient strategy to treat the perchlorate contaminated waters.

Bioaugmentation of RBC Biofilm by Phenol-Degrading Rhodococcus sp. EL-GT

Son, Hong Joo,Kim, Keun Ki,Kim, Han Su,Kim, Yong Gyun 밀양대학교 농업기술개발연구소 2001 農業技術開發硏究所報 Vol.5 No.1

본 연구에서는 페놀분해세균 Rhodococcus sp. EL-GT를 접종한 회전원판법에 의하여 페놀함유 폐수의 페놀제거 효율 및 생성된 생물막 특성에 대하여 조사하였다. 회전원판은 10rpm 및 수리학적 체류시간 7시간의 조건으로 작동시켰다. 시간 경과에 따라 Rhodococcus sp. EL-GT를 접종한 실험구의 페놀제거 효율 및 SCOD 제거효율이 증가하였으며, 질산화 과정도 촉진되었다. This research was performed to investigate biofilm growth and the comparison of phenol removal efficiency by RBC(Rotating Biological Contactor) using Rhodococcus sp. EL-GT augmented reactor and unaugmented reactor. The experiment was carried out at rotating speed of 10 rpm and hydraulic retention time of 7 h. As time passed, the phenol removal efficiency, SCOD removal efficiency and nitrification of the bioflm augmented reactor were increases significantly. Unaugmented reactor's phenol removal efficiency and SCOD removal efficiency were decreased after 10 days when the influent phenol concentration of 1,200 mg/l.

Bioactive Compounds / Food Microbiology : < Note >: A Leaf-Inhabiting Endophytic Bacterium, Rhodococcus sp. KB6, Enhances Sweet Potato Resistance to Black Rot Disease Caused by Ceratocystis fimbriata(s)

( Chi Eun Hong ),( Haeyoung Jeong ),( Sung Hee Jo ),( Jae Cheol Jeong ),( Suk Yoon Kwon ),( Donghwan An ),( Jeong Mee Park ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.3

Rhodococcus species have become increasingly important owing to their ability to degrade a wide range of toxic chemicals and produce bioactive compounds. Here, we report isolation of the Rhodococcus sp. KB6, which is a new leaf-inhabiting endophytic bacterium that suppresses black rot disease in sweet potato leaves. We determined the 7.0 Mb draft genome sequence of KB6 and have predicted 19 biosynthetic gene clusters for secondary metabolites, including heterobactins, which are a new class of siderophores. Notably, we showed the first internal colonization of host plants with Rhodococcus sp. KB6 and discuss its potential as a biocontrol agent for sustainable agriculture.

Natural attenuation, biostimulation 및 Rhodococcus sp. EH831을 이용한 bioaugmentation에 의한 디젤 오염 토양의 정화

이은희 ( Eun Hee Lee ),강연실 ( Yeon Sil Kang ),조경숙 ( Kyung Suk Cho ) 한국미생물생명공학회(구 한국산업미생물학회) 2011 한국미생물·생명공학회지 Vol.39 No.1

3가지 종류의 생물정화법인 natural attenuation (NA), biostimulation (BS) 및 bioaugmentation (BA) 방법을 디젤로 오염된 토양을 정화하기 위해 적용하여, 각 방법에 의한 정화효율과 미생물 활성을 계면활성제 첨가 유무(Tween80)에 따라 비교하였다. 토양 정화 초기 단계에서는 Rhodococcus sp. EH831을 접종원으로 이용하는 BA 방법에 의한 토양 정화효율이 가장 좋았다. 3가지 생물정화방법 모두에서 계면활성제 첨가는 토양 정화효율에 영향을 미치지 않았다. 토양의 탈수소활성(DHA)과 잔류 총석유계탄화수소(TPHs) 농도는 음의 상관관계를 보였다: DHA (μg-TPF·g-dry soil-1 d-1) = -0.02 × TPHs concentration (mg-TPHs·kg-dry soil-1) + 425.76 (2500≤ TPHs concentration≤20000, p < 0.01). Three bioremediation methods, natural attenuation (NA), biostimulation (BS) and bioaugmentation (BA) were applied to remediate diesel-contaminated soil, with their remediation efficiencies and soil microbial activities compared both with and without surfactant (Tween 80). BA treatment employing Rhodococcus sp. EH831 was the most effective for the remediation of diesel-contaminated soil at initial remediation stage. On the addition of surfactant, no significant effect on the remediation performance was observed. A negative correlation was found between the dehydrogenase activity (DHA) and residual concentration of total petroleum hydrocarbons (TPHs) at below 20,000 mg-TPHs ·kg-dry soil-1, as follows: DHA (μg-TPF(Triphenylformazan) kg-dry soil-1 d-1) = -0.02 × TPHs concentration (mg-TPHs·kg-dry soil-1) + 425.76 (2500 ≤ TPHs concentration ≤ 20000, p < 0.01).

Plant Terpene-Induced Expression of Multiple Aromatic Ring Hydroxylation Oxygenase Genes in Rhodococcus sp. Strain T104

Kim, Byung-Hyuk,Oh, Eun-Taex,Ahn, Yeong-Hee,Koh, Sung-Cheol The Microbiological Society of Korea 2003 The journal of microbiology Vol.41 No.4

Recent studies have shown that some of the PCB (polychlorinated biphenyl)-degraders are able to effectively degrade PCB in the presence of monoterpenes, which act as inducers for the degradation pathway. Rhodococcus sp. T104, an effective PCB degrader, has been shown to induce the degradation pathway by utilizing limonenes, cymenes, carvones, and pinenes as sole carbon sources which can be found in the natural environment. Moreover, the strain T104 proved to possess three separate oxidation pathways of limonene, biphenyl, and phenol. Of these three, the limonene can also induce the biphenyl degradation pathway. In this work, we report the presence of three distinct genes for aromatic oxygenase, which are putatively involved in the degradation of aromatic substrates including biphenyl, limonene, and phenol, through PCR amplification and denaturing gradient gel electrophoresis (DGGE). The genes were differentially expressed and well induced by limonene, cymene, and plant extract A compared to biphenyl and/or glucose. This indicates that substrate specificity must be taken into account when biodegradation of the target compounds are facilitated by the plant natural substrates.

Rhodococcus에 의한 농흉 및 전이성 농양 1례

이영민 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3

Rhodococcus sp. are a common pathogen in herbivores, particularly domesticated species. It rarely procedures human diseases, and infected subjects are usually persons with impaired immunity. Among rhodococci, R.equi is the most commonly isolated species and pneumonia is the most common form of disease produced by R.equi in humans, occupying in approximately two thirds of cases in the series. Inhalation is the major route of inoculation, and infections in humans primarily involve the lungs. The natural habitant of R.equi is soil, particularly soli associated with herbivore manure. Persons exposed to horses, livestock farmers, and gardeners have a higher risk for infection. Skin and soft tissue abscesses have been reported only in humans with disseminated disease, although primary skin infection with R. equi do occur in animals. Dissemination from focal sites(usually the lung) to brain, skin, paraspinal tissue, and bone has been described in human infection. We report the case of a patient with Rt. empyema and metastatic Rt. flank subcutaneous and brain abscess by Rhodococcus sp.

유류 분해 근권세균 Rhodococcus sp. 412와 옥수수를 활용한 유류 오염 토양의 정화

홍선화,박혜림,고우리,유재준,조경숙 한국미생물·생명공학회 2007 한국미생물·생명공학회지 Vol.35 No.3