갈근의 장기투여가 주정중독 환자의 음주 욕구에 미치는 영향

김성곤,김명정,윤종태 大韓神經精神醫學會 1995 신경정신의학 Vol.34 No.6

Effect of chronic administration of Radix puerariae on alcohol craving in alcoholic patients was studied. The subjects were hospitalized male alcoholics recovered from acute intoxication and withdrawal symptoms. They were divided into three groups, each administered with placebo, Radix puerariae 2 grams daily, and Radix puerariae 4 grams daily, respectively for 4 weeks. Alcohol craving was measured at the starting day, 14the and 28th day of the study by means of visual anaologue scale(VAS), Likert scale 1 and Likert scale 2. The results were as follows : 1) Alcohol craving as measured with all the above scales decreased significantly after 2 and 4 weeks of Radix puerariae administration, in a dose of 4 grams a day. 2) Decrease of alcohol craving as measured with VAS and Likert scale 1 after 4 weeks of Radix puerariae administration in a dose of 4 grams daily was significantly greater than that after 2 weeks of Radix puerariae administration in the same dose. 3) Alcohol craving as measured with VAS and Likert scale 1 decreased significantly after 4 weeks Radix puerariae administration in a dose of 2 grams a day. 4) Alcohol craving as measured with VAS decreased significantly after 2 weeks of Radix puerariae administration in a dose of 2 grams daily, and it further decreased significantly after 4 weeks of Radix puerariae administration in the same does. 5) Depression as measured with Beck Depression Inventory improved significantly after 4 weeks of administration of the palcebo, Radix puerariae 2 grams and 4 grams daily and also after 2 weeks of administration of the placebo and Radix puerariae 2 grams daily. 6) However, depression measured with Hamilton Rating Scale for Depression did not change after 2 and 4 weeks of administration of the placebo and Radix puerariae. 7) Food intake did not change during 28 days of the experiment in all the three groups. From these results, the authors suggest that chronic administration of Radix puerariae may decrease alcohol craving in patients with alcoholism and the decrease may not be a result of alleviation of depression or change in nonspecific food intake.

Effect of Puerariae radix on c-Fos Expression in Hippocampus of Alcohol-Intoxicated Juvenile Rats

JANG, Mi-Hyeon,SHIN, MIn-Chul,LEE, Taeck-Hyun,BAHN, Geon-Ho,SHIN, Hye-Sook,LIM, Sabina,KIM, Ee-Hwa,KIM, Chang-Ju WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Alcohol consumption is known to cause substantial neuronal loss in several regions of the brain. In Oriental medicine, medications based on Puerariae radix have been known to be of efficacy in the treatment of alcohol-related problems. In the present study, the effect of the aqueous extract of Puerariae radix on the expression of c-Fos, an immediate early gene whose expression is sometimes used as a marker for stimulus-induced changes in the metabolic activity of neurons, in the hippocampus of acutely alcohol-intoxicated juvenile rats was investigated via immunohistochemistry. In the first part of the experiment, Sprague-Dawley rats were divided into six groups: the control group, the alcohol-treated group, the alcohol, and 0.3 ㎎/㎏ Puerariae radix-treated group, the alcohol-and 3㎎/㎏ Puerariae radix-treated group, the alcohol-and 30㎎/㎏ Puerariae radix-treated group, the alcohol-and 300㎎/㎏ Puerariae radix-treated group. In the second part of the experiment, animals were divided into four groups the control group, the 30㎎/㎏ Puerariae radix-treated group, the alcohol-treated group, and the alcohol-and 30㎎/㎏ Puerariae radix-treated group. From the results, it was demonstrated that alcohol administration significantly decreases the number of Fos-positive cells in the various regions of the hippocampus, and Puerariae radix treatment inhibits the alcohol-induced suppression of the expression of Fos in the hippocapus in a dose-dependent manner. Puerariae radix exerted no significant effect on Fos expression in the hippocampus of normal rats. The results presented in this study suggest that Puerariae radix may alleviate alcohol-induced disruption of hippocampal functions.

Puerariae Radix induces Angiogenesis in vitro and in vivo

Choi Do-Young,Kang Jung-Won,Cho Eun-Mi,Lee Jae-Dong,Hyh Jeong-Eun,Yang Ha-Ru,Baek Yong-Hyeon,Kim Deog-Yoon,Cho Yoon-Je,Kim Kang-il,Park Dong-Suk 대한침구의학회 2005 대한침구의학회지 Vol.22 No.2

Background & Objective : Angiogenesis consists of the proliferation, migration, and differentiation of endothelial cells, and angiogenic factors and matrix protein interactions modulate this process. The aim of this study was to determine whether Puerariae radix could induce angiogenic activity in human umbilical vein endothelial cells (HUVECs).Methods : The angiogenic activity of Puerariae radix were evaluated by using BrdU assay, chemotactic migration assay, tube formation assay, measurement of bFGF in HUVECs, and Matrigel plug assay in mice.Results : Puerariae radix significantly increased HUVECs proliferation in a dose-dependent manner. In addition, Puerariae radix increased migration and tube-like formation in HUVECs. Interestingly,the expression of basic fibroblast growth factor (bFGF), an angiogenesis-stimulating growth factor, was dose-dependently increased by Puerariae radix. The angiogenic activity of Puerariae radix was confirmed using an in vivo Matrigel angiogenesis model, showing promotion of blood vessel formation. Conclusion : Puerariae radix significantly induces angiogenesis in vitro and in vivo. These results suggest that Puerariae radix is a potent angiogenic agent, and a promising drug, for the induction of neovascularization.

Puerariae radix increases Alcohol-induced Suppresssed Cell Proliferation and Expression of Nitric Oxide Synthase in Dentate Gyrus of Rats

Jang, Mi Hyeon,Lee, Taeck Hyun,Shin, Min Chul,Lim, Baek Vin,Kim, Hyun Bae,Lim, Sabina,Kim, Jin Woo,Lee, Choong Yeol,Kim, Ee Hwa,Kim, Chang Ju 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.1

Traditionally, aqueous extracts of Puerariae radix had been used for the treatment of alcohol-related problems. In the present study, the effect of Puerariae radix on cell proliferation and expression of nitric oxide synthase (NOS) in the dentate gyrus of alcohol-intoxicated rats were investigated via 5-bromo-2'-deoxyuridine (BrdU) immunohistochemistry and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry, respectively. Sprague-Dawley rats weighing 150±10g were divided into four groups: the control group, the Puerariae radix-treated group, the alcohol-treated group, and the alcohol- and Puerariae radix-treated group. The numbers of both BrdU-positive and NADPH-d-positive cells in the dentate gyrus were inhibited significantly by alcohol administration, while Puerariae radix treatment was shown to increase those numbers. In this study, it was revealed that Puerariae radix possesses protective effect against alcohol-induced suppressed new cell formation and NOS expression in the dentate gyrus. Based on the results, it is possible that NO, which might play an important role in the regulation of cell proliferation, is a major target of the toxic effects of alcohol.

갈근 열수 추출물이 알루미늄을 투여한 흰쥐의 혈청 효소 활성도와 호르몬에 미치는 영향

한성희,신미경 한국식생활문화학회 2005 韓國食生活文化學會誌 Vol.20 No.1

This study was designed to investigates the effects of Korean pueraris radix water extract in Al(Aluminum) administered rats. Forty-eight male Sprague-Dawley rats weighing 100±10g were used for this experiment and divided into following 6 groups; control group, 3% pueraria radix in water extract group, 1000 and 2000ppm Al group, 1000 and 2000ppm Al group with 3% pueraria radix in water extract group. The Al administered rats were given 1000 and 2000 ppm of Al₂(SO₄)₃ disolved in the distilled water. The Al content in the rats tissue of Al administered group was lower than in the rats tissue of AI group with 3% pueraria radix in water extract group. Plasma levels of renin and aldosterone activity was increased by Al administration group, compared with 3% pueraria radix in water extract group and Al administred group. Glutamate oxaloacetate transaminase(GOT) and Glutamate pyruvate transaminase(GPT) were increased in Al-administered group and lower in the 3% extracts of pueraria radix in water extract group. Lactate dehydrogenase(LDH) was lower in the 3% extracts of pueraria radix-Al group than in the Al group. This results suggested that pueraria radix in water extract group has a lowering effects on the accumulation of Al and it is belived that the pueraria radix in extracted water group has some protective effects to Al administered in rats, but the mechanism of these effects was obscure.

Proteins isolated of Pueraria Radix possible to cause allergenic react with immunoglobulin E in human sera

Kim, Yumi,Chung, Won Seok,Jang, Hyeung-Jin THE KOREAN SOCIETY OF TOXICOGENOMICS AND TOXICOPRP 2018 MOLECULAR AND CELLULAR TOXICOLOGY Vol. No.

Backgrounds: Allergies occur when immunoglobulin E (IgE), which is part of the body's immune system, binds to molecules. A protein in the foods, plants or pollen is usually the problem. This causes the release of inflammatory chemicals such as histamine. There are a few cases where allergies are cause by proteins derived from herbal medicine, but we do not know yet what protein causes the immune react to IgE. Methods: Here, we demonstrate to confirm if the Pueraria Radix at the proteomic level triggers IgE immunoreactivity. Finally, we present the molecules of the Pueraria Radix-derived protein that trigger the IgE immune response. To prove our hypothesis, we used proteomic tools and SDS-PAGE stained with Coomassie blue and identified proteins using LC-MS/MS. Proteins were separated from Pueraria Radix and allergic reaction sera were selected. Results: We found three positive sera that showed an immune response to Pueraria Radix protein. Positive sera demonstrated a similar pattern of IgE reactivity and mostly restricted to a 50-70 kDa band. Therefore, we have identified number of different IgE binding proteins. Therefore, IgE binding proteins were identified such as enolase, hypothetical protein, DING protein and glycosyl transferase. Conclusion: The results imply that Pueraria Radix can act as an allergen given the identification of IgE binding proteins among the isolated proteins of Pueraria Radix.

Proteins isolated of Pueraria Radix possible to cause allergenic react with immunoglobulin E in human sera

김유미,정원석,Hyeung-Jin Jang 대한독성 유전단백체 학회 2018 Molecular & cellular toxicology Vol.14 No.2

Backgrounds: Allergies occur when immunoglobulin E (IgE), which is part of the body’s immune system, binds to molecules. A protein in the foods, plants or pollen is usually the problem. This causes the release of inflammatory chemicals such as histamine. There are a few cases where allergies are cause by proteins derived from herbal medicine, but we do not know yet what protein causes the immune react to IgE. Methods: Here, we demonstrate to confirm if the Pueraria Radix at the proteomic level triggers IgE immunoreactivity. Finally, we present the molecules of the Pueraria Radix-derived protein that trigger the IgE immune response. To prove our hypothesis, we used proteomic tools and SDS-PAGE stained with Coomassie blue and identified proteins using LC-MS/ MS. Proteins were separated from Pueraria Radix and allergic reaction sera were selected. Results: We found three positive sera that showed an immune response to Pueraria Radix protein. Positive sera demonstrated a similar pattern of IgE reactivity and mostly restricted to a 50-70 kDa band. Therefore, we have identified number of different IgE binding proteins. Therefore, IgE binding proteins were identified such as enolase, hypothetical protein, DING protein and glycosyl transferase. Conclusion: The results imply that Pueraria Radix can act as an allergen given the identification of IgE binding proteins among the isolated proteins of Pueraria Radix.

카드뮴을 급여한 흰쥐에서 갈근 열수 추출액의 해독작용효과

정영희,한성희,신미경 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.4

3% 갈근 열수 추출액 급여와 카드뮴을 흰쥐에게 4주 동안 급여 한 후 간과 신장 조직의 카드뮴 함량, renin 호르몬 농도, 혈청중의 GPT, GOT 및 LDHase의 함량을 조사하였다. 식이섭취랑에서 대조구인 24.62g에 비하여, 3% 갈근 열수 추출액 급여군은 23.41g으로 카드뮴 급여군은 23.76g에 비하여, 갈근 열수 추출액급여군과 카드뮴 병합 급여군은 22.25g으로 감소하였으나 유의한 차이는 없었다. 체중 증가량은 대조군이 124.5.g이고, 3% 갈근 열수 추출액급여군은 127.22g으로 대조군에 비하여 감소하였다. 카드뮴 급여군은 107.57g에 비하여, 갈근 열수 추출액급여군과 음용수의 카드뮴 병합 급여군은 128.80g으로 유의적으로 증가하였다. 식이효율은 카드뮴 급여군과 갈근-카드뮴 병합 급여군 간에는 유의성이 인정되었다. 간의 무게에서 대조군에 비하여 갈근 열수 추출액 급여군이 약간 감소하였으나 유의한 차이는 없었으며 카드뮴 공급군에 비하여 갈근-카드뮴 병합 급여군이 유의적으로 증가하였다. 신장 무게는 카드뮴 공급군에 비하여 갈근-카드뮴 병합 급여군이 유의적으로 증가하였다. 간 조직내에서의 카드뮴 함량은 대조군이 0.15ug/g으로 갈근 열수 추출액급여군은 0.14ug/g에 비하여 별다른 차이를 보이지 않았다. 카드뮴 급여군은 3.04ug/g에 비하여 갈근-카드뮴 음용수 병합 급여군은 2.87ug/g 유의적으로 감소하였다. 신장 조직내에서 대조군은 0.21ug/g이었으나, 갈근 열수 추출액급여군은 0.23ug/g으로 별다른 차이는 없었다. 카드뮴 급여군은 6.48ug/g에 비하여 갈근 열수 추출액급여군과 카드뮴 병합 급여군은 4.42ug/g 4.57ug/g으로 유의적으로 감소하였다. 혈장 호르몬인 renin 농도는 갈근차 음용수군은 16.73ngAl/mL/hr으로 대조군인 15.89ngAl/mL/hr에 비해서 증가하였다. 카드뮴 급여군은 25.72ngAl/mL/hr으로 카드뮴과 갈근 열수 추출액 병합 급여군인 18.57ngAl/mL/hr에 비하여 유의성 있게 증가하였다. GOT는 대조군이 96.16U/L에 비하여 갈근차 단독 급여군은 96.44U/L로 약간 증가하였다. 카드뮴 급여군은 154.40U/L인데 비하여 카드뮴과 갈근 열수 추출액 병합 급여군이 118.80U/L으로 유의적으로 감소하였다. GPT는 대조군이 56.50UL에 비하여, 갈근 열수 추출액 급여군은 63.46U/L으로 약간 증가하였으나 유의한 차이는 없었다. 카드뮴 급여군은 73.54U/L인데 비하여 갈근 열수 추출액 급여군과 카드뮴 병합 급여군은 69.80U/L으로 유의적으로 감소하였다. LDHase는 대조군이 179.00U/L, 갈근 열수 추출액급여군은 198.60U/L으로 대조군에 비하여 감소하였으나 유의한 차이는 나지 않았다. 카드뮴 급여군은 264.30U/L인데 비하여 갈근 열수 추출액급여군과 카드뮴 동시 병합 급여군은 227.30U/L으로 유의적으로 감소하였다. 카드뮴에 중독된 흰쥐에 대한 갈근 추출물의 해독 효과를 알아보는 본 연구에서 50ppm카드뮴액과 함께 3% 갈근 추출액 급여한 흰쥐의 체중증가량과 사료 섭취 효율을 50ppm의 카드뮴만을 급여한 흰쥐의 이들 측정값과 비교할 때 유의적인 차이가 없었다. 그러나 50ppm의 카드뮴액과 함께 3% 갈근 추출액을 급여한 흰쥐군은 50ppm의 카드뮴액만을 급여한 흰쥐군과 비교하여 신장내 카드뮴 함량과 GPT 및 LDH 활성도, reinn 활성도가 유의적으로 감소되었고 신장 무게는 정상 흰쥐와 같은 수준으로 회복하였고 GOT 활성도 역시 정상 흰쥐와 같은 수준으로 감소를 보여 갈근이 카드뮴 중독 흰쥐에게 신장 등의 장기내 카드뮴 축적의 감소로 카드뮴 중독 작용의 경감 효과를 갖는 것으로 나타났다. This study was designed to investigates the effects of Korean pueraris radix water extract in Cd(cadmium) administered rats. Forty male Sprague-Dawley rats weighing 100±10g were used for this experiment and divided into following 4 groups; control group, 3% pueraria radix in water extract group, 50 ppm Cd group, 50 ppm C group with 3% pueraria radix in water extract group. The Cd administered rats were given 50 ppm of CdCl_2 · 2H_2O disolved in the distilled water. The Cd content in the rats tissue of Cd administered group was lower than in the rats tissue of Cd group with 3% pueraria radix in water extract group. Plasma levels of renin activity was increased by Cd administration group, compared with 3% pueraria radix in water extract group and Cd administred group. Glutamate oxaloacetate transaminase(GOT) and Glutamate pyruvate transaminase(GPT) were increased in Cd-administered group and lower in the 3% extracts of puerariz radix in water extract group. Lactate dehydrogenase(LDHase) was lower in the 3% extracts of pueraria radix-Cd group than in the Cd group. This results suggested that pueraria radix in water extract group, has a lowering effects on the accumulation of Cd and it is belived that the pueraria radix in water extract group has some protective effects to Cd administered in rats, but the mechanism of these effects was obscure.

Puerariae radix increases Alcohol-induced Suppressed Cell Proliferation and Expression of Nitric Oxide Synthase in Dentate Gyrus of Rats

Jang Mi Hyean,Lee Taeck Hyun,Shin Min Chul,Lim Baek Vin,Kim Hyun Bae,Lim Sabina,Kim Jin Woo,Lee Cheong Yeal,Kim Ee Hwa,Kim Chang Ju The Physiological Society of Korean Medicine and T 2002 동의생리병리학회지 Vol.16 No.1

Traditionally, aqueous extracts of Puerariae radix had been used for the treatment of alcohol-related problems. In the present study, the effect of Puerariae radix on cell proliferation and expression of nitric oxide synthase (NOS) in the dentate gyrus of alcohol-intoxicated rats were investigated via 5-bromo-2' -deoxyuridine (BrdU) immunohistochemistry and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry, respectively. Sprague-Dawley rats weighing 150 ± 10 g were divided into four groups: the control group, the Puerarias radix-treated group, the alcohol-treated group, and the alcohol- and Puerariae radix-treated group. The numbers of both BrdU-positive and NADPH-d-positive cells in the dentate gyrus were inhibited significantly by alcohol administration, while Puerariae radix treatment was shown to increase those numbers. In this study, it was revealed that Puerariae radix possesses protective effect against alcohol-induced suppressed new cell formation and NOS expression in the dentate gyrus. Based on the results, it is possible that NO, which might play an important role in the regulation of cell proliferation, is a major target of the toxic effects of alcohol.

갈근류 생약의 puerarin 함량분석 및 품질평가

황완균,정운정,오창환,송효남,이종필,성락선,제금련,장승엽,고성권 한국생약학회 2007 생약학회지 Vol.38 No.2

study was carried out to obtain the basic information that can be used to index arrowroots.(Pueraria species)in fourteen regions of China and Korea. The puerarin content in various arrowroots produced in the different areas were quan-titatively analysed by HPLC. The average of puerarin content of the ‘Galgeun’ (Pueraria lobata) was higher than that of the‘Bungalgeun’ (Pueraria thomsoni) produced in China and Korea. As a result, the order of the puerarin content was 1) Korean.(P. lobata, 0.609%), 2) Chinese puerariae radix.(P. lobata, 0.546%), 3) Chinese puerariae radix.(P. thomsonii,0.066%). This result showed that the content of puerarin in the ‘Galgeun’ (P. lobata) are about 10 times higher than those ofthe ‘Bungalgeun’ (P. thomsonii).Key words HPLC, Pueraria lobata, Pueraria thomsonii, puerariae radix, puerarin.. (.. ). .. (Leguminosae). . Pueraria lobata(WILLD.) OHWI(Pueraria thunbergiana BENTH.). ... ... ... , .. ... .. .. ... , . 5mm. .