포유자돈에 있어서 포유자돈용 요구르트의 급여가 생산성, 혈액특성 및 설사지수에 미치는 영향

조진호,이장형,Cho, J.H.,Lee, J.H. 국립한국농수산대학교 교육개발센터 2013 현장농업연구지 = Journal of practical agricultural resear Vol.15 No.1

Offspring of 14 sows were used to evaluate the effect of yogurt substituted for creep feed on growth performance, diarrhea score and blood profiles. The piglets of sows were randomly allocated into 1 of 2 treatments (1 treatment separated piglets of 7 sows). The dietary treatments were as followed: CF, creep feed; YG, yogurt. The creep feed and yogurt diets were given from 3 d after piglets were born to the day of weaning (21 d after piglets were born). The body weight and feed consumption were weighed and recorded at d 0, 7, 14, and 21 after piglets were born for calculating average daily gain (ADG) and average daily feed intake (ADFI). At the weaning day, two piglets were randomly chosen from each pen and bled via jugular venipuncture to obtain blood samples for WBC and IgG measurement. All data were subjected to the GLM procedures of SAS (2002) as a randomized complete block design, with pen as the experimental unit. Differences among dietary treatments were separated by Duncan's multiple range test, and P < 0.05 was considered statistically significant. No significant difference (P > 0.05) were observed in number of born piglets, weaning pigs, survival rate, body weight of piglets at d 0, 7, 14, and 21, ADG and ADFI. At d 21, piglets fed with creep feed had a higher (P < 0.05) IgG compared with piglets fed with yogurt. No significant difference (P > 0.05) was observed in WBC among treatments at d 21. In conclusion, yogurt substituted for creep feed had no adverse effect on growth performance in lactating piglets.

Simultaneous feeding of calcium butyrate and tannin extract decreased the incidence of diarrhea and proinflammatory markers in weaned piglets

Maito Camila Demarco,Melo Antonio Diego Brandão,Oliveira Angela Cristina da Fonseca de,Genova Jansller Luiz,Filho Jair Rodini Engracia,Macedo Renata Ernlund Freitas de,Monteiro Kelly Mazutti,Webe 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.1

Objective: This study was conducted to investigate the effect of associating calcium butyrate with tannin extract, compared to an antimicrobial on the growth performance, incidence of diarrhea, intestinal histology, immune-expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor α (TNF-α) in piglets. Methods: Seventy-two piglets (36 barrows and 36 gilts) weaned at 28±2 d and initial body weight of 7.17±1.07 kg were allocated to 3 treatments in a randomized complete block design with 8 replicates per treatment and 3 animals per experimental unit. Treatments were composed of NC, negative control: basal diet without additives; PC, positive control: basal diet + 40 mg/kg of colistin sulfate; or BT, basal diet + calcium butyrate + tannin extract. The butyrate and tannin inclusion levels were 0.15% in the pre-starter phase and 0.075% in the starter phase. Incidence of diarrhea was monitored daily, and on d 14 and 35 of experiment, 1 animal from each experimental unit was slaughtered to collect intestinal samples. Results: No significant differences were observed for growth performance. The butyrateand tannin-based additive resulted in reduced (p<0.05) incidence of diarrhea in piglets during d 1 to 14 and d 1 to 35 in comparison with the other treatments. Piglets that consumed the diet containing the calcium-butyrate and tannin showed a lower (p<0.05) crypt depth in the duodenum than those receiving the NC treatment at 14 d of experimentation. The BT treatment provided a lower (p<0.05) immune-expression of COX-2 at 14 d and TNF-α at 35 d in the duodenum. Conclusion: Association between calcium butyrate and tannin extract resulted in a significant decrease in the incidence of diarrhea and inflammatory process in the duodenum of piglets. Therefore, calcium-butyrate combined with tannin could be a part of an alternative program to reduce the use of antimicrobials in the diet of weaned piglets. Objective: This study was conducted to investigate the effect of associating calcium butyrate with tannin extract, compared to an antimicrobial on the growth performance, incidence of diarrhea, intestinal histology, immune-expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor α (TNF-α) in piglets.Methods: Seventy-two piglets (36 barrows and 36 gilts) weaned at 28±2 d and initial body weight of 7.17±1.07 kg were allocated to 3 treatments in a randomized complete block design with 8 replicates per treatment and 3 animals per experimental unit. Treatments were composed of NC, negative control: basal diet without additives; PC, positive control: basal diet + 40 mg/kg of colistin sulfate; or BT, basal diet + calcium butyrate + tannin extract. The butyrate and tannin inclusion levels were 0.15% in the pre-starter phase and 0.075% in the starter phase. Incidence of diarrhea was monitored daily, and on d 14 and 35 of experiment, 1 animal from each experimental unit was slaughtered to collect intestinal samples.Results: No significant differences were observed for growth performance. The butyrate-and tannin-based additive resulted in reduced (p<0.05) incidence of diarrhea in piglets during d 1 to 14 and d 1 to 35 in comparison with the other treatments. Piglets that consumed the diet containing the calcium-butyrate and tannin showed a lower (p<0.05) crypt depth in the duodenum than those receiving the NC treatment at 14 d of experimentation. The BT treatment provided a lower (p<0.05) immune-expression of COX-2 at 14 d and TNF-α at 35 d in the duodenum.Conclusion: Association between calcium butyrate and tannin extract resulted in a significant decrease in the incidence of diarrhea and inflammatory process in the duodenum of piglets. Therefore, calcium-butyrate combined with tannin could be a part of an alternative program to reduce the use of antimicrobials in the diet of weaned piglets.

Effects of Sodium Sulfite and Extrusion on the Nutritional Value of Soybean Meal in Piglets Weaned at 21 Days

Piao, X.S.,Jin, J.,Kim, J.D.,Kim, J.H.,Sohn, K.S.,Hyun, Y.,Han, In K. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.7

A total of 80 weaned piglets (Landrace $\times$ Yorkshire $\times$ Large White) were used in a 28-day growth assay to detennine the optimal inclusion level of sodium sulfite ($Na_{2}SO_{3}$) as an extrusion enhancer of soybean meal for nursery piglets. piglets (21 d of age, 6.04 kg of BW) were grouped into 4 treatments in a completely randomized block design. Treatments were: 1) Extruded SBM (Control), 2) Extruded SBM with 0.5% $Na_{2}SO_{3}$ (0.5 ESBM), 3) Extruded SBM with 1.0% $Na_{2}SO_{3}$ (1.0 ESBM) and 4) Extruded SBM with 1.5% $Na_{2}SO_{3}$ (1.5 ESBM). Each treatment has 4 replicates of 5 heads per pen. In phase I (d 0 to 14), diets supplied 3,400 kcal ME/kg, 23% crude protein, 1.65% lysine, 0.50% methionine, 0.9% Ca and 0.8% P. Phase II (d 14 to 28) diets contained 3,300 kcal ME/kg, 21% crude protein, 1.45% lysine, 0.45% methionine, 0.9% Ca and 0.8% P. For d 0 to 14, piglets fed 1.5 ESBM had greater ADG, ADFI and FCR compared to piglets fed control and 0.5 ESBM diet. ADG was significantly higher in piglets fed 1.5 ESBM diet than other groups (p<0.05) except 1.0 ESBM. In phase II (d 14 to 28), there was no significant differences in production traits among treatments. For overall period (d 0 to 28), piglets fed diets with high sodium sulfite grew faster than piglets fed control and 0.5 ESBM diets. The highest ADG and the best FeR were obtained in piglets fed diets with 1.5 ESBM during the entire period. Piglets fed 1.5 ESBM diet showed significantly higher crude protein digestibility than 0.5 ESBM (p<0.05) at d 14 post-weanling, but not at d 28 post-weanling. There were no significant differences in digestibilities of total amino acids. In conclusion, the addition level of 1~1.5% sodium sulfite for SBM extrusion could be favorable for rate and efficiency of growth in weaning pigs.

포유자돈에서 돼지써코바이러스 2형이 돼지유행성설사 바이러스 감염에 미치는 영향 -1. 혈청학적 결과, 형광항체검사 및 RT-PCR 검사

김문 ( Wen Jin ),김영훈 ( Yeong Hun Kim ),한정희 ( Jeong Hee Han ) 한국동물위생학회 2009 한국동물위생학회지 (KOJVS) Vol.32 No.3

The purpose of this study was to evaluate the effect of a subsequent infection of PCV2 on piglets with PEDV. The results obtained were as follows: Antibodies against PCV2 and PEDV were detected at 24, 36, 48, 60 and 72h postinfection. And the antibody titers of alone infections with PEDV were gradually reduced and increased from 60 hpi to 72 hpi. Whereas, the antibody titers of dual infections with PCV2 and PEDV were gradually reduced all the time. PEDV antigens were detected at 24, 36, 48, 60 and 72 hpi, being seen almost exclusively in feces and small intestines from PEDV-infected piglets and PCV2- coinfected piglets. The detection rate of PEDV in feces and jejunum tissues by RT-PCR were 94.9% and 91.1% in dual infections and 87.1% and 83.6% in alone infections with PEDV, respectively. In dual infected piglets, significantly more PEDV antigens were detected in the feces and small intestines tissues at 24 hpi (P?0.05) than in the same feces and tissues of the alone infected piglets. Thereafter, at 72 hpi significantly more PEDV antigens (P?0.05) was detected in the jejunal tissues of the dual infected piglets with than of alone PEDV-infected piglets. The detection rate of PEDV antigen in the duodenum, jejunum and ileum by IFA were 91.3%, 91.3% and 83.3% in dual infected piglets and 75.0%, 83% and 75% in alone infected piglets, respectively. Intense and specific fluorescence signals were more often seen within jejunal villous enterocytes in dual infected piglets than alone infected piglets.

포유자돈에서 돼지써코바이러스 2형이 돼지유행성설사 바이러스 감염에 미치는 영향 -2. 임상증상, 병리조직학적 검사 및 면역조직학적 검사

김문 ( Wen Jin ),김영훈 ( Yeong Hun Kim ),한정희 ( Jeong Hee Han ) 한국동물위생학회 2009 한국동물위생학회지 (KOJVS) Vol.32 No.3

The purpose of this study was to evaluate the effect of a subsequent infection of PCV2 on piglets with PEDV. In clinical signs, the signs observed in dual infected with PEDV and PCV2 piglets and alone infected with PEDV piglets ranged from diarrhoea to vomiting and dehydration. Dual infected piglets developed signs of anorexia, vomiting and watery diarrhoea within 12 hpi. Nevertheless alone infected piglets caused pasty diarrhea at first. In mortality, dual infections showed 25%, but alone infections showed 8.3%, respectively. In gross findings, piglets of dual infections group appeared the severe findings of congestion, distension of lumen, contaning curdes of undigested milk in stomach milder than those of alone infections group. In histopathological findings, piglets of dual infection group appeared the more severe findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. Significant (P?0.05) decrease in VH : CD ratio in dual infected piglets compared to piglets from alone PEDV infections. In immunohistochemical findings, strong hybridization signals in dual infections were seen in areas of moderate to severe villous atrophy or vacuolation with positive cells arranged continuously over the villi. In the lumen, exfoliated enterocytes were strongly positive in dual infected piglets. The number of PEDV-positive cells in dual infected pigs was significantly higher than that in alone PEDV-infected piglets.

The Effect of Complementary Access to Milk Replacer to Piglets on the Activity of Brush Border Enzymes in the Piglet Small Intestine

Wang, J.F.,Lundh, T.,Westrom, B.,Lindberg, J.E. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.11

The activity of brush border enzymes (sucrase, lactase and maltase) in the piglet small intestine was evaluated as well as piglet performance during the weaning period in the present study. There were two treatment groups: Piglets of six litters were fed dry feed plus milk replacer (Group M) and of six litters fed dry pelleted feed (Group C). One piglet from each litter was sacrificed on day 3 before weaning, and day 3, 10 and 17 postweaning, respectively. Providing milk replacer caused an increased piglet live weight at weaning (p<0.001) and until termination of the experiment (p<0.001). A slightly higher (p<0.16) level of protein was measured in the jejunum of group M piglets as compared with group C piglets. Before weaning the activity of lactase was high in the jejunum of group C piglets. The activity of lactase in the jejunum was lowered in the jejunum of group C piglets and in distal jejunum of group M piglets during the postweaning period as compared with pre-weaning period (p<0.05). Lowered activity of lactase in the distal jejunum of piglets was found at day 10 and 17 postweaning, respectively. No treatment differences were found in the activity of lactase in the piglet jejunum. No treatment differences were seen in the activity of maltase and sucrase in the piglet jejunum also. However, weaning caused a higher activity of sucrase in the distal jejunum of group M piglets as compared with pre-weaning period. In conclusion, providing milk replacer to piglets caused an improved growth performance. Feeding milk replacer did not influence the activity of lactase, maltase and sucrase in the jejunum of piglets. Weaning resulted in a markedly lowered activity of lactase, while no dramatic changes in the activity of maltase took place during the period around weaning.

포유자돈의 합사가 모돈과 자돈의 체표면 온도 변화에 미치는 영향

김두완(Doo-Wan Kim),김영화(Young-Hwa Kim),김광식(Kwang-Sik Kim),김기현(Ki-Hyun Kim) 한국산학기술학회 2017 한국산학기술학회논문지 Vol.18 No.1

본 연구는 포유기간 중 이복자돈과의 합사가 모돈과 자돈의 체표면 온도 변화에 미치는 영향을 조사하기 위하여 실시하였다. 임신모돈 12두를 공시하여 6두는 개별 분만펜에 위치시켜 분만 후 이유시까지 복당 사육을 유지하였으며(대조구), 6두는 중간 칸막이의 제거가 가능한 가변형 분만펜에서 3복이 한 펜이 되도록 분만 11일차에 칸막이를 제거하여 이유시까지 유지하였다(처리구). 합사 30분 후에 열화상카메라를 이용하여 모돈 및 자돈의 체표면 온도를 측정하였다. 분만 후 포유모돈의 평균 체표면 온도는 처리구에서 37.1°C로 대조구의 36.3°C보다 유의적으로 높은 것으로 나타났고 (p<0.05), 최고온도에서는 두 처리간 유의적인 차이가 없었다. 이와 대조적으로, 포유 자돈의 평균 체표면 온도는 포유기 합사에 의해 유의적으로 감소하였으며(대조구 37.5, 처리구 36.0°C; p<0.01). 최고온도에서 또한 대조구와 처리구에서 각각 39.4°C와 39.1°C로 유의적인 차이는 아니지만 대조구에서 낮은 경향으로 관찰되었다(p=0.079). 결론적으로 포유기간 중에 이복자돈과의 합사에 의해 모돈의 체표면 온도는 상승되지만, 자돈의 체표면 온도는 감소하는 것으로 관찰되었다. 더 나아가 체표면 온도와 심부온도와의 상관관계를 구명하고, 합사에 의한 모돈 및 자돈의 생리적 변화에 대한 연구가 필요할 것으로 사료된다. This study was conducted to investigate the effects of mixing with unfamiliar piglets on changes in the body surface temperature of sows and piglets during the suckling period. A total of 123 pigs (12 sows and 111 piglets) were used for this study. A control group of piglets of the same litter was maintained in the farrowing pen and compared to a treatment group of piglets of three different litters mixed by removing the partition in the farrowing pen. In the treatment group, mixing of piglets was performed at 10:00 a.m. on day 11 after parturition, and the body surface temperature of sows and piglets was taken using a thermo-graphic camera at 30 minutes after mixing. In the case of sows, the average surface temperature of the treatment group (37.1°C) was significantly higher than that of the control (36.3°C; p<0.05); however, the hot spot temperatures did not differ significantly between groups. In contrast, the average surface temperature of piglets was significantly decreased by mixing (37.5 and 36.0°C in the control and treatment, respectively; p<0.01). Moreover, the hot spot temperature tended to be lower in the treatment (39.1°C) than the control (39.4°C), although there was no significant difference (p=0.079). These results suggest that mixing of unfamiliar piglets during the suckling period leads to changes in the body surface temperature of sows and piglets. In the future, the correlation between body surface temperature and body core temperature should be analyzed, and additional studies investigating the effects of mixing on the physiological changes in sows and piglets are required.

포유자돈에 있어서 포유자돈용 발효유의 급여가 생산성, 혈액특성, 및 설사지수에 미치는 영향

김승철(Kim-Seung Cheol),김현수(Hyun-Soo Kim),허정민(Jung-Min Heo),김인호(In-Ho Kim) 충남대학교 농업과학연구소 2015 농업과학연구 Vol.42 No.4

본 연구는 포유자돈용 발효유의 급여가 포유자돈의 생산성, 설사지수, 혈액특성에 미치는 영향을 알아보기 위해 시험을 실시하였다. 사양 시험은 3산차 모돈(Landrace × Yorkshire)은 14두를 공시하여, 2처리구 처리당 7두로 분만 후 부터 이유시까지 3주간 사양시험을 실시하였다. 시험설계는 1) CF (Creep feeding), 2) FM (fermented milk)로 실시하였다. 포유자돈용 발효유의 급여가 생산성에 있어서 유의적 차이는 보이지 않았으나, 생시체중이 입붙임 사료구와 비교하여 수치상 낮았으나, 이유체중에서는 포유자돈용 발효유를 급여한 구가 300 g 증가한 결과를 나타내었다. 포유자돈용 발효유의 급여시 설사지수에 있어서는 처리구간 유의적 차이가 없었다. 혈액 특성에 있어서는 혈액내 백혈구 함량에 있어서는 처리구간 유의적인 차이를 나타내지 않았지만(P>0.05), 혈액 내 면역 글로불린 함량에 있어서는 입붙임 사료를 급여한 처리구가 포유자돈용 발효유를 급여한 처리구보다 높게 나타났다(P<0.05). 이와 같은 결과는 입붙임 사료는 탄수화물, 단백질, 지방 및 면역관련 물질을 함유하고 있으나, 본 연구에서 사용된 포유자돈용 발효유는 장내 미생물균총에 유익한 영향을 미치는 유산균은 함유하고 있으나, 다른 면역물질 및 첨가제를 함유하고 있지 않아 혈액 면역세포에 영향을 미치지 않은 것으로 판단된다. 결론적으로 포유기간 동안 입붙임 사료를 대신하여 포유자돈용 발효유를 급여하여도 생산성에 부정적인 영향을 미치지 않는 것으로 판단되며, 입붙임 사료를 대체할 수 있는 물질로 급여 가능할 것으로 사료된다. In the present study, offsprings of 14 sows were used to evaluate the effect of fermented milk substituted for creep feed to analyze its effect on growth performance, diarrhea score and blood profiles. The piglets of sows were randomly allocated into 1 of 2 treatments (1 treatment separated piglets of 7 sows). The dietary treatments includes: CF (creep feed) and FM (fermented milk). The CP and FM diets were given from 3 d after piglets were born to the day of weaning (21 d after piglets were born). No significant difference (P>0.05) were observed in number of born piglets, weaning pigs, survival rate, body weight of piglets at d 0, 7, 14, and 21, ADG and ADFI. At d 21, piglets fed with CP had a higher (P<0.05) IgG compared with piglets fed with FM. No significant difference (P>0.05) was observed in WBC among the treatments at d 21. At d 6, piglets fed with CP had a lower (P<0.05) number of diarrhea piglets compared to piglets fed with FM. In conclusion, FM substituted for CP had no adverse effect on growth performance in lactating piglets.

The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition

Guevarra, Robin B.,Hong, Sang Hyun,Cho, Jin Ho,Kim, Bo-Ra,Shin, Jiwon,Lee, Jun Hyung,Kang, Bit Na,Kim, Young Hwa,Wattanaphansak, Suphot,Isaacson, Richard E.,Song, Minho,Kim, Hyeun Bum BioMed Central 2018 Journal of animal science and biotechnology Vol.9 No.-

<P><B>Background</B></P><P>Understanding the composition of the microbial community and its functional capacity during weaning is important for pig production as bacteria play important roles in the pig’s health and growth performance. However, limited information is available regarding the composition and function of the gut microbiome of piglets in early-life. Therefore, we performed 16S rRNA gene and whole metagenome shotgun sequencing of DNA from fecal samples from healthy piglets during weaning to measure microbiome shifts, and to identify the potential contribution of the early-life microbiota in shaping piglet health with a focus on microbial stress responses, carbohydrate and amino acid metabolism.</P><P><B>Results</B></P><P>The analysis of 16S rRNA genes and whole metagenome shotgun sequencing revealed significant compositional and functional differences between the fecal microbiome in nursing and weaned piglets. The fecal microbiome of the nursing piglets showed higher relative abundance of bacteria in the genus <I>Bacteroides</I> with abundant gene families related to the utilization of lactose and galactose. <I>Prevotella</I> and <I>Lactobacillus</I> were enriched in weaned piglets with an enrichment for the gene families associated with carbohydrate and amino acid metabolism. In addition, an analysis of the functional capacity of the fecal microbiome showed higher abundances of genes associated with heat shock and oxidative stress in the metagenome of weaned piglets compared to nursing piglets.</P><P><B>Conclusions</B></P><P>Overall, our data show that microbial shifts and changes in functional capacities of the piglet fecal microbiome resulted in potential reductions in the effects of stress, including dietary changes that occur during weaning. These results provide us with new insights into the piglet gut microbiome that contributes to the growth of the animal.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1186/s40104-018-0269-6) contains supplementary material, which is available to authorized users.</P>

Effect of Saccharomyces cerevisiae boulardii on sows’ farrowing duration and reproductive performance, and weanling piglets’ performance and IgG concentration

( Haiqing Sun ),( Fernando Bravo De Laguna ),( Shuai Wang ),( Fengju Liu ),( Liang Shi ),( Haidi Jiang ),( Xiaoxia Hu ),( Peng Qin ),( Jiajian Tan ) 한국축산학회 2022 한국축산학회지 Vol.64 No.1

We studied the effects of Saccharomyces cerevisiae boulardii CNCM I-1079 (LSB) supplemented to lactating sows on reproductive traits and farrowing duration and to piglets from day 7 of life on post-weaning performance and IgG concentration. Ninety-six Landrace × Yorkshire sows started the trial 5 days before the expected farrowing date. Sows were distributed into 2 groups according to parity number and backfat thickness: control (CON: regular lactation diet) and LSB (CON + LSB at 2 × 10⁹ colony forming units [CFU]/kg of feed). Seven days after birth, litters were randomly selected from each group and supplemented creep feed with or without LSB at 2 × 10⁹ CFU/kg. At weaning, piglets from CON sows were shifted to a commercial farm and allocated to 14 pens in groups of 25 piglets/pen according to the creep feed supplemented during lactation. Piglets followed a 3-phase feeding program: creep, pre-starter and starter, with or without LSB at 2 × 10⁹ CFU/kg LSB in creep and pre-starter, and 1 × 10⁹ CFU/kg LSB in starter. The piglets were vaccinated against classical swine fever on days 41 and 72 of life. One day before each vaccination and at the end of the trial, blood samples were collected from 15 randomly selected piglets per treatment and assessed for total IgG. Supplemented sows with non-supplemented litters displayed the lowest backfat thickness loss during lactation (p < 0.05). The LSB supplementation shortened farrowing duration (p < 0.05) and increased feed intake (p < 0.05) during the first week of lactation. The LSB-fed piglets were heavier at the end of creep (p < 0.05), pre-starter (p < 0.05), and the trial (p < 0.05); grew faster during creep (p < 0.05), starter (p < 0.05), and overall (p < 0.05); and displayed an improved feed conversion ratio during creep (p < 0.05). Total IgG content was higher at days 40 (p < 0.05) and 71 (p < 0.05) in LSB-fed piglets. We conclude that supplementing sows with Saccharomyces cerevisiae boulardii CNCM I-1079 from late gestation until weaning shortens farrowing duration, increases feed intake, and minimizes backfat losses during lactation. When supplemented to piglet diet, post-weaning performance is improved. This improvement observed could be linked to a better immune status, as suggested by the higher IgG.