Photothermal therapy using gold nanoparticles on the acne of an Asian patient demographic: Preliminary study

( Tae Jun Park ),( Jae Yeong Jeong ),( Ko Eun Kim ),( Jong Heon Park ),( Hwa Jung Ryu ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1

Background: Acne is a common skin disease that occurs in pilosebaceous units and is often prevalent in adolescence. There are many acne treatments, but there are also known side effects to these treatments, such as antibiotics resistances, teratogenicity, and irritation. Therefore, it is necessary to develop a more effective and safe alternative treatment for managing acne in patients of all ages. Objectives: This study aims to confirm the effect of the treatment with gold photothermal therapy on acne. Methods: Twelve patients who visited the dermatologic clinic with moderate to severe acne vulgaris were included in the study. All patients received three successive gold photothermal therapy at 1~2-week intervals. The number of papules, pustules, and comedones before and after treatment, along with overall improvement, were assessed. In the four patients, a biopsy of the acne lesion was taken in acne lesion before and one month after the last treatment. Results: Significant reduction of acne lesion was observed after the use of the gold photothermal therapy (papule; p<0.001, pustule; p<0.001, comedone; p=0.001). In histopathological findings, a decrease in the inflammatory cell infiltration and fibrotic change of dermis were observed after the gold photothermal therapy. Conclusion: Gold photothermal therapy showed significant clinical and histological improvement on acne vulgaris without serious adverse effects in Asian patient populations.

광열치료의 열 투여량에 따른 세포 자멸사에 대한 수치적 연구

김무중(Moojoong Kim),김관택(Gwantaek Kim),김동혁(Donghyuk Kim),이희진(Heejin Kim),유재석(Jaisuk Yoo),김동권(Dong-Kwon Kim),김현정(Hyun Jung Kim) 대한기계학회 2018 대한기계학회 춘추학술대회 Vol.2018 No.12

Photothermal therapy(PTT) is a therapy for selective ablation of the tumor by using a photothermal effect. However, in spite of using the photothermal effect, which is one of the fields of heat transfer, the research on heat transfer for photothermal therapy is insufficient. In photothermal therapy, GNPs (gold nanoparticles) are used as a photothermal enhancer to selective ablation of tumor. At this time, since the photothermal effect are different depending on the characteristics of the GNPs, and thermal and optical conditions of NIR laser, it is important to find an optimal condition suitable for selective ablation for effective photothermal therapy. In previous photothermal therapy studies, the temperature distribution in tumor tissues has been studied qualitatively. However, the correlation between the death of tumor tissue and thermal and optical conditions of NIR laser has not been quantitatively studied in terms of heat transfer. Therefore, in this study, the conditions (e.g. intensity, beam radius) of the lasers satisfying the apoptosis temperature (43℃ ~ 50℃ , which can effectively ablate tumors) were obtained for various sizes of tumor (width: 2mm ~ 10mm, depth: 0.5mm ~ 4.5mm) using numerical analysis. And quantitative analysis of the apoptosis rate of tumor tissue according to the thermal and optical conditions of photothermal therapy was performed.

In vitro and In Vivo photothermal cancer therapy with pH tunable Bio-imaging by using IR825 integrated fluorescence nanoparticles

강은비,박성영,이기백 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0

We report a combined diagnostic fluorescence for bioimaging and therapeutic fluorescence for photothermal therapy (PTT) within an integrate hybrid nanoparticles can simultaneously and accurately diagnosis and ablates the tumor. This new class triggered photothermal therapeutic agents for pH responsive cancer cells target bio-imaging constructed using near-infrared dye (NIR) IR825, polymer conjugated anchored reduced graphene oxide (rGO) hybrid nanoparticles. Using hybrid nanoparticles administered PTT agents, the time-dependent in vivo tumor accumulated hybrid nanoparticles generated require photothermal heat, which caused dominant suppression of tumor growth. The ability to maximize tumor therapy and to monitor treatment condition in target area explores an appealing interest for the successful application of photothermal therapy. This was supported by Korea National University of Transportation in 2015.

Engineering tumor-specific catalytic nanosystem for NIR-II photothermal-augmented and synergistic starvation/chemodynamic nanotherapy

Shuixiu Zhou,Jiahuan Xu,Yanfei Dai,Yan Wei,Liang Chen,Wei Feng,Yu Chen,Xuejun Ni 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: As an emerging therapeutic modality, chemodynamic therapy (CDT), converting hydrogen peroxide (H2O2) into highly toxic reactive oxygen species (ROS), has been developed for tumor-specific therapy. However, the deficiency of endogenous H2O2 and high concentration of glutathione (GSH) in the tumor microenvironment (TME) weaken the CDT-based tumor-therapeutic efficacy. Herein, a photothermal-enhanced tumor-specific cascade catalytic nanosystem has been constructed on the basis of glucose oxidase (GOD)-functionalized molybdenum (Mo)-based polyoxometalate (POM) nanoclusters, termed as GOD@POMs. Methods: GOD@POMs were synthesized by a facile one-pot procedure and covalently conjugation. Then, its structure was characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). In addition, ultraviolet-visiblenear-infrared (UV-vis-NIR) absorption spectrum and infrared thermal camera were applied to evaluate the catalytic and photothermal performance, respectively. Moreover, to confirm the therapeutic effects in vitro, cell counting kit-8 (CCK-8) assay, live/dead staining and ROS staining were performed. Furthermore, the biosafety of GOD@POMs was investigated via blood routine, blood biochemistry and hematoxylin and eosin (H&E) staining in Kunming mice. Besides, the C6 glioma tumor-bearing mice were constructed to evaluate its anti-tumor effects in vivo and its photoacoustic (PA) imaging capability. Notably, RNA sequencing, H&E, TdT-mediated dUTP nick end labeling (TUNEL) and Ki-67 staining were also conducted to disclose its underlying anti-tumor mechanism. Results: In this multifunctional nanosystem, GOD can effectively catalyze the oxidation of intratumoral glucose into gluconic acid and H2O2, achieving the cancer starvation therapy. Meanwhile, the generated gluconic acid decreases the pH in TME resulting in POM aggregation, which enables PA imaging-guided tumor-specific photothermal therapy (PTT), especially in the second near-infrared (NIR-II) biological window. Importantly, the Mo (VI) sites on POM can be reduced to Mo (V) active sites in accompany with GSH depletion, and then the post-produced Mo (V) transforms in situ overproduced H2O2 into singlet oxygen (1O2) via Russell mechanism, achieving self-enhanced CDT. Moreover, the PTT-triggered local tumor temperature elevation augments the synergistic nanocatalytic-therapeutic efficacy. Conclusions: Consequently, the integration of GOD-induced starvation therapy, H2O2 self-supply/GSH-depletion enhanced Mo-based CDT, and POM aggregation-mediated PTT endow the GOD@POMs with remarkable synergistic anticancer outcomes with neglectable adverse effects.

Chlorin e6 conjugated copper sulfide nanoparticles for photodynamic combined photothermal therapy

Bharathiraja, S.,Manivasagan, P.,Moorthy, M.S.,Bui, N.Q.,Lee, K.D.,Oh, J. ELSEVIER SCIENCE B.V., AMSTERDAM 2017 PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY Vol.19 No.-

The photo-based therapeutic approaches have attracted tremendous attention in recent years especially in treatment and management of tumors. Photodynamic and photothermal are two major therapeutic modalities which are being applied in clinical therapy. The development of nanomaterials for photodynamic combined with photothermal therapy has gained significant attention for its treatment efficacy. In the present study, we designed chlorin e6 (Ce6) conjugated copper sulfide (CuS) nanoparticles (CuS-Ce6 NPs) through amine functionalization and the synthesized nanoparticles act as a dual-model agent for photodynamic therapy and photothermal therapy. CuS-Ce6 NPs showed enhanced photodynamic effect through generation of singlet oxygen upon 670nm laser illumination. The same nanoparticles exerted thermal response under an 808nm laser at 2W/cm<SUP>2</SUP>. The fabricated nanoparticles did not show any cytotoxic effect toward breast cancer cells in the absence of light. In vitro cell viability assay showed a potent cytotoxicity in photothermal and photodynamic treatment. Rather than singular treatment, the photodynamic combined photothermal treatment showed an enhanced cytotoxic effect on treated cells. In addition, the CuS-Ce6 NPs exert a photoacoustic signal for non-invasive imaging of treated cells in tissue-mimicking phantom. In conclusion the CuS-Ce6 NPs act as multimodal agent for photo based imaging and therapy.

Recent advances in near-infrared-II hollow nanoplatforms for photothermal-based cancer treatment

Li Zhang,Gerile Oudeng,Feiqiu Wen,Guangfu Liao 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Near-infrared-II (NIR-II, 1000–1700 nm) light-triggered photothermal therapy (PTT) has been regarded as a promising candidate for cancer treatment, but PTT alone often fails to achieve satisfactory curative outcomes. Hollow nanoplatforms prove to be attractive in the biomedical field owing to the merits including good biocompatibility, intrinsic physical-chemical nature and unique hollow structures, etc. On one hand, hollow nanoplatforms themselves can be NIR-II photothermal agents (PTAs), the cavities of which are able to carry diverse therapeutic units to realize multi-modal therapies. On the other hand, NIR-II PTAs are capable of decorating on the surface to combine with the functions of components encapsulated inside the hollow nanoplatforms for synergistic cancer treatment. Notably, PTAs generally can serve as good photoacoustic imaging (PAI) contrast agents (CAs), which means such kind of hollow nanoplatforms are also expected to be multifunctional all-in-one nanotheranostics. In this review, the recent advances of NIR-II hollow nanoplatforms for single-modal PTT, dual-modal PTT/photodynamic therapy (PDT), PTT/chemotherapy, PTT/catalytic therapy and PTT/gas therapy as well as multi-modal PTT/chemodynamic therapy (CDT)/chemotherapy, PTT/chemo/gene therapy and PTT/PDT/CDT/starvation therapy (ST)/immunotherapy are summarized for the first time. Before these, the typical synthetic strategies for hollow structures are presented, and lastly, potential challenges and perspectives related to these novel paradigms for future research and clinical translation are discussed.

NIR light-powered halloysite-based nanomotors for CT imaging diagnosis and synergistic chemo-photothermal cancer therapy

Xiaolei Zhang,Ziying Wang,Yangsai Lyu,Jia Li,Kun Song,Ningning Xing,Dickon H.L. Ng 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.116 No.-

Near-infrared (NIR) light-powered nanomotors with active motion have attracted extensive attention inthe chemo-photothermal therapy of cancer. However, there are also some limitations such as complicatedsynthesis process, low permeability of drugs and viscous physiological environment. Herein, wedeveloped a facile route to synthesize novel NIR light-powered DOX/Au-m-HTAS nanomotor based onnatural halloysite (HNTs) for active chemo-photothermal therapy. Such nanomotor exhibited NIR lightcontrolled on/off motion with a maximum speed of 64.5 lm/s. Owing to the synergetic NIR lightpoweredmotion and chemo-photothermal therapy, DOX/Au-m-HTAS nanomotor could effectivelyadhere tumor cells, leading to deeper cell penetration, higher cellular uptake, and better ablation oftumor cells. Meanwhile, DOX/Au-m-HTAS nanomotors also had enhanced ability for CT imaging of localtumor, demonstrating the enhanced anticancer efficiency. This strategy provided a new insight into thechemo-photothermal synergistic therapy of tumors.

Nanoparticle Platforms for Combined Photothermal and Photodynamic Therapy

박지호,Jeongmin Oh,Hwanjun Yoon 대한의용생체공학회 2013 Biomedical Engineering Letters (BMEL) Vol.3 No.2

Phototherapy is a promising strategy for cancer treatment due to its selective and localized therapeutic effect by laser irradiation. Photothermal therapy damages malignant cells by using heat converted from light by an agent. On the other hand, photodynamic therapy uses photosensitizers that become cytotoxic upon irradiation with laser light at excitation wavelength. As singular treatment of each phototherapy showed some limitations, there have been significant efforts to enhance therapeutic effect by combining photothermal and photodynamic therapy. Here we review recent developments of nanoparticle platforms, in which inorganic nanostructures (photothermal therapy) are integrated with photosensitizers (photodynamic therapy) for combined phototherapeutic effect.

Metal Ion Releasing Gold Nanoparticles for Improving Therapeutic Efficiency of Tumor Targeted Photothermal Therapy

Park Jung Hwan,정의영,Lim Hyeonji,이주로,Joung Yoon Ki,유태경,방석호 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.2

BACKGROUND: Owing to the tumor-targeted migration capacity of human mesenchymal stem cells (hMSCs), they have been combined with nanoparticles for photothermal therapy. However, the low viability of hMSCs following transplantation remains a problem. Here, we developed iron (Fe) ion-releasing gold (Au) nanoparticles (IIAuNPs) for advanced tumor-targeted photothermal therapy using hMSCs. METHODS: IIAuNPs were designed to undergo degradation under low pH conditions, such as the endosomal microenvironment, for Fe ion release in hMSCs. After evaluating the properties of IIAuNP, the IIAuNP concentration for treating hMSCs was optimized in terms of cytotoxicity. In vitro cell migration and antiapoptotic factor secretion were observed in hMSCs. Additionally, IIAuNPs-treated hMSCs were intravenously injected into tumor-bearing mice, and enhanced tumor targeting based on improved cell viability and cell migration was evaluated. Three days after the injection, the mice were irradiated with 660 nm laser to confirm the enhanced photothermal effect. RESULTS: In vitro studies revealed that treating hMSCs with an optimum concentration of IIAuNPs enhanced cell migration and anti-apoptotic gene expression through intracellular Fe ion delivery. The viability of hMSCs under hypoxic cell culture conditions that mimic the in vivo microenvironment was also improved when hMSCs were treated with IIAuNPs, compared to hMSCs without IIAuNPs treatment. IIAuNPs-treated hMSCs showed significantly enhanced tumor-targeting efficiency and subsequent photothermal effect compared to hMSCs without IIAuNP treatment. CONCLUSION: Our results suggest that our metal-ion-releasing photothermal nanoparticles may provide a promising platform for future photothermal therapies and related applications.

rGO nanomaterial-mediated cancer targeting and photothermal therapy in a microfluidic co-culture platform

Mun Seok Gyu,최형우,이종민,임재현,하장호,Kang Min-Jung,Kim Eun-Joong,Kang Lifeng,정봉근 나노기술연구협의회 2020 Nano Convergence Vol.7 No.10

We developed the microfluidic co-culture platform to study photothermal therapy applications. We conjugated folic acid (FA) to target breast cancer cells using reduced graphene oxide (rGO)-based functional nanomaterials. To characterize the structure of rGO-based nanomaterials, we analyzed the molecular spectrum using UV–visible and Fourier-transform infrared spectroscopy (FT-IR). We demonstrated the effect of rGO-FA-based nanomaterials on photothermal therapy of breast cancer cells in the microfluidic co-culture platform. From the microfluidic co-culture platform with breast cancer cells and human umbilical vein endothelial cells (HUVECs), we observed that the viability of breast cancer cells treated with rGO-FA-based functional nanomaterials was significantly decreased after near-infrared (NIR) laser irradiation. Therefore, this microfluidic co-culture platform could be a potentially powerful tool for studying cancer cell targeting and photothermal therapy.