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- 주제어 : Photobiological Hydrogen Production (검색결과 5 건)
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단세포성 해양남세균 종주를 이용한 광생물학적 수소생산 기술
박종우,김재만,이원호,Park, Jong-Woo,Kim, Jae-Man,Yih, Won-Ho 한국해양학회 2009 바다 Vol.14 No.1
광생물학적 수소생산 잠재력을 가진 다양한 미소생물 가운데, 남세균은 21세기의 수소경제 시대에 적합한 생물군으로 오랫동안 알려져 왔다. 광생물학적으로 수소에너지를 생산하게 될 경우, 해양 단세포성 질소고정 남세균은 남세균류의 하부 분류군들 가운데 가장이상적인 종류의 하나로 평가되고 있다. 단세포성 질소고정 남세균을 이용한 수소생산 기술을 개발하기 위해 반드시 고려해야 할 3가지 사항은 1) 자연계에 존재하는 최우수 수소생산 종주의 확립 2) 광생물학적 수소생산을 뒷받침하는 종주-특이적 최적조건의 탐색 3) 유전학적 방법을 이용한 수소생산 종주의 개량 등이다. 본고에서는 광생물학적 수소생산기술의 상업화를 향한 최근의 연구 개발 추세를 돌아보고, 해양 단세포성 남세균 종주를 이용한 광생물학적 수소생산 기술 분야에서 한국의 세계선도적 지위 확보를 위해서는 향후 10-15년간 집중적인 연구 개발이 절실함을 제안하고자 한다. Among various microscopic organisms producing photobiological hydrogen, cyanobacteria have long been recognized as the promising biological agents for hydrogen economy in 21 century. For photobiological production of hydrogen energy, marine unicellular $N_2$-fixing cyanobacteria have been evaluated as an ideal subgroup of Cyanophyceae. To develope the hydrogen production technology using unicellular $N_2$-fixing cyanobacteria, 3 important factors are pre-requisite: 1) isolation of the best strain from marine natural environment, 2) exploration on the strain-specific optimal conditions for the photobiological hydrogen production, and finally 3) application of the molecular genetic tools to improve the natural ability of the strain to produce hydrogen. Here we reviewed the recent research & development to commercialize photobiological hydrogen production technology, and suggest that intensive R&D during next 10-15 years should be imperative for the future Korean initiatives in the field of the photobiological hydrogen production technology using photosynthetic marine unicellular cyanobacterial strains.
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김봉진,김종욱 한국수소및신에너지학회 2008 한국수소 및 신에너지학회논문집 Vol.19 No.4
This paper deals with an economic evaluation of domestic photobiological hydrogen production. We evaluate the economic feasibility of domestic photobiological hydrogen production utilizing green algae and cyanobacteria. In addition, we make some sensitivity analysis of hydrogen production prices by changing the values of input factors such as the price of a photo-bioreactor and the value of solar to hydrogen efficiency. The estimated hydrogen production price of the two-step indirect biophotolysis was 12,099won/kgH2. It is expected that the hydrogen production price by the two-step indirect biophotolysis can be reduced to 2,143won/kgH2 if the solar to hydrogen efficiency is increased to 10% and the price of a photo-bioreactor is decreased to $25/㎡. The two-step indirect biophotolysis is evaluated as uneconomical at this time, and we need to enhance the solar to hydrogen efficiency and to reduce the prices of the photo-bioreactor and system facilities.
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국내 연안산 질소고정 단세포 남세균 종주의 광생물학적 수소생산력
박종우,명금옥,이원호 한국수소및신에너지학회 2010 한국수소 및 신에너지학회논문집 Vol.21 No.2
Photobiological hydrogen production by nitrogen-fixing unicellular cyanobacteria has long been considered to be an environmentally sound and very promising method for the future supply of renewable clean energy. We tried to find out the optimum cell concentration for H2 production in each of the two new Korean nitrogen-fixing unicellular cyanobacterial strains to compare with Synechococcus sp. strain Miami BG043511. The two Korean strains, Cyanothece sp. KNU CB MAL-031 and KNU CB MAL-058, were isolated from Korean west coasts. Cell concentrations up to 17 billion cells ml-1 were applied to the tests. High cell concentration over 15 billion cells ml-1 resulted in drastically reduced H2 production in all the three strains. The two domestic strains, however, produced 2-3 time more hydrogen than Synechococcus sp. Miami BG043511 at cell concentrations of 5-10 billion cells ml-1. At lower cell concentrations than 2 billion cells ml-1, MAL-031 exhibited highest H2 production followed by Miami BG043511, with far less production in MAL-058. Present result suggests that Cyanothece sp. MAL-CB031 might be one of the ideal nitrogen-fixing unicellular cyanobacterial strains for the photobiological hydrogen production.
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박종우,안세희,김형섭,이원호 한국수소및신에너지학회 2011 한국수소 및 신에너지학회논문집 Vol.22 No.5
Under a daily photoperiod of 14h light and 10h dark synchronization of cell cycle in Korean Cyanothece spp. strains and Synechococcus sp. strain Miami BG043511 was analyzed as to be applicable to enhanced hydrogen production. For all strains peaks of double cell were observed during the light period of a daily cycle. Peaks of maximal cell size measured by a coulter counter appeared at the peak of double cells observed under light microscope reconfirming the synchronization of daily cell cycle. The cell cycle synchronization became weakened within two days when treated with continuous illumination. Rapid detection of the peak time of double cell percentage by coulter counters may contribute to quasi-realtime feedback control for efficient production of photobiological hydrogen by unicellular cyanobacterial strains.
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한국 연안산 질소고정 단세포 남세균 종주의 최적 성장 및 수소생산 온도
박종우,김형섭,이원호 한국수소및신에너지학회 2013 한국수소 및 신에너지학회논문집 Vol.24 No.1
Photobiological hydrogen production by nitrogen-fixing unicellular cyanobacteria has long been considered to be an environmentally sound and very promising method for the future supply of renewable clean energy. Using six Korean nitrogen-fixing unicellular cyanobacterial strains and the Synechococcus sp. strain Miami BG043511 we performed cultivation experiments to find out the strain-specific optimal temperature for population growth and H2 production. Under 20℃ the population growth of all the tested strains was significantly retarded in contrasts to the faster and higher growth under 25, 30 or 35℃. The highest growth rates in all the 7 strains were measured under 30℃ while the maximal biomass yields were under 30℃ (strains CB-MAL 026, 054, and 055) or 35℃ (strains 002, 031, 058, and Miami BG043511). The difference between the maximal biomass yields at 30℃ and 35℃ was not greater than 10%. The quantity of photobiologically produced H2 was only slight larger under 35℃ than that under 20℃. Our result may suggest a two-step process of H2 production which includes rapid and sizable production of biomass at 30℃ and the following high H2 production at 20℃ by the test strains of marine nitrogen-fixing unicellular cyanobacteria.
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