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- 주제어 : PUVA treatment (검색결과 3 건)
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김춘미,최미경 이화여자대학교 생명과학연구소 1992 생명과학연구논문집 Vol.3 No.-
The effect of radioprotective ginseng protein fraction on DNA repair capacity was determined by measuring the amount of ^3H-thymidine incorporated into DNA in the process of repair synthesis for UV damaged DNA. CHO-K1 cells were prepared whose semiconservative replication was inhibited by trimethylpsoralen plus near-UV(PUVA) treatment. When the cells were exposed to UV light alone, the DNA repair capacity was increased at first and then decreased as UV dose increased. However, when the ginseng fration was treated to the cells, the DNA repair capacity was kept increasing regardless of UV dose increament. When the concentration of protein contained in the added fraction was ioncreased gradually, the repair capacity was also increasea aimosi lireatly showing dose-response relationship of the effect. These results suggest that the enhancement of DNA repair capaclty of the cell can be one of the mechanisms of radioprotection by the ginseng fraction.
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Effects of Radioprotectors on DNA Repair Capacity of Tumor Cells
Kim, Choon-Mi,Kim, Mi-Kyung The Pharmaceutical Society of Korea 1993 Archives of Pharmacal Research Vol.16 No.4
Three cell lines, CHO, L929 and B16 which are non-tumorigenic and cancer cells, respectively, were first tested for their survival in the presence of radioprotective ginseng protein fraction(GPF0. The influence of three radioprotectors-CPF, cysteamine, and 1-Methyl-2-bis[(2-methylthio)vinyl] quinolinium iodide (MVQI) on DNA repair capacity of UV damaged cells survival test, the GPF showed higher cytotoxicity in L929 and B16 than in CHO cells. However, the degree of cell killing was also investigated by measuring $^3H$-thymidine incorporation of PUVA treated cells. In cell survival test, the GPF showed higher cytotoxicity in L929 and B16 than in CHO cells. However, the degree of cell killing was not high enough to consider it as an antitumorigenic agent. Variable results were obtained in the effects on DNA repair capacity depending on the protectors and cell lines used. In pretreatment, the presence of GPF and MVOI brought about a sinificant increase in the capacity in both CHO and B16 cells. However, in L929, the enhancing effect was not shown. In all three cell lines, cysteamine showed lower repair capacity than control, suggesting the primary damage reduction in stronger enhancing effects in L929 and B16 cells, while it was weaker in CHO cells. Here also cystemine hsowed a very little or no increase in the capacity in all three cell lines. These results demonstrate that GPF has mild cytotoxicity in tumorignic cells and that GPF and MVQI enhance DNA repair capacity of UV damaged cells, whether they are tumorigenic or not. On the other hand, cysteamine shows only damage reduction effect. Celles of different genetic origin seem to give different responses to the modifier and different modifiers may possibly work by different mechanisms.
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