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CRISPR-Cas9 system을 이용한 Kluyveromyces marxianus 17694-DH2 균주의 자일로스 소비속도 증진
권덕호,박중희,정덕열,박재범,박동민,강경곤,최서영,김수린,하석진 한국생물공학회 2019 KSBB Journal Vol.34 No.4
The third-generation gene editing technology, CRISPR-Cas9 system, is derived from the bacterial immune system. These CRISPR-Cas9 systems have recently been used to mutate yeast gene or replace cleavage sites with other DNA. In this study, CRISPR-Cas9 system was applied to delete PHO13 gene of Kluyveromyces marxianus. As a result, only one strain of three transformants, that the CRISPR-Cas9 system was applied, was confirmed partial deletion of PHO13 gene. Sequencing of the PHO13 gene revealed the deletion of cytosine at position 457, which resulted in premature termination of translation as compared to that from the parental strain. The ΔPHO13 strain, K. marxianus 17694-DH2, showed 18.29% and 21.28% improvement in xylose consumption and ethanol production from xylose, respectively, as compared to those form the parental strain.
Rhee, Young Ha,Kim, Young Hwan,Shin, Kwang-Soo Elsevier 2006 Enzyme and microbial technology Vol.38 No.3
<P><B>Abstract</B></P><P>The characteristics of an extracellular poly(3-hydroxyoctanoate) (PHO) depolymerase purified from the marine isolate <I>Pseudomonas luteola</I> M13-4 were elucidated. The enzyme consisted of a monomeric subunit, having a molecular mass of 28kDa and isoelectric point of 6.0. The optimum reaction pH and temperature were 10.0 and 40°C, respectively. Its hydrolyzing activity was significantly inhibited by phenylmethylsulfonyl fluoride (PMSF), suggesting the involvement of a serine as an active site amino acid. The enzyme was able to hydrolyze various types of medium-chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) as well as various chain-length <I>p</I>-nitrophenyl (PNP) esters of fatty acids. The amino acid sequence of the tryptic peptide contained a lipase box pentapeptide sequence, G-I-S-S-G. The N-terminal amino acid sequence of the enzyme exhibited 60–68% similarity to those of other MCL-PHA depolymerases.</P>