바이오 응용을 위한 초음파 및 광학 기반 다중 모달 영상 기술

이문환,박희연,이경수,김세웅,김지훈,황재윤 한국음향학회 2023 韓國音響學會誌 Vol.42 No.5

이 연구는 초음파 광학 영상 기반의 다중 모달 영상 기술에 대한 최신 연구 동향과 응용 가능성에 대해 조사하였다. 초음파 영상은 실시간 영상 기능을 가지고 있으며 인체에 상대적으로 안전한 특성으로 인해 의료 분야에서 다양한질병의 진단에 사용되고 있다. 그러나 초음파 영상은 해상도가 낮은 한계가 있어 진단 정확도를 향상시키기 위해 다른광학 영상과의 결합을 통한 다중 모달 영상 기술 개발 연구가 진행되고 있다. 특히 초음파 광학 영상 기반의 다중 모달영상 기술은 각각의 영상 기법의 장점을 극대화하고 단점을 보완함으로써 질병 진단 정확도를 향상시킬 수 있는 수단으로 사용되고 있다. 이러한 기술은 초음파의 실시간 영상 기능과 광간섭 단층 영상 융합 기술, 초음파 광음향 다중 모달영상 기술, 초음파 형광 다중 모달 영상 기술, 초음파 형광 시정수 다중 모달 영상 기술 및 초음파 분광 다중 모달 영상기술 등 다양한 형태로 제안되고 있다. 본 연구에서는 이러한 초음파 광학 영상 기반의 다중 모달 영상 기술의 최신 연구동향을 소개하고, 의학 및 바이오 분야에서의 응용 가능성을 조사하였다. 이를 통해 초음파와 광학 기술의 융합이 어떻게 진행되고 있는지에 대한 통찰력을 제공하고, 의료 분야에서의 진단 정확도 향상을 위한 새로운 접근 방식에 대한기반을 마련하였다. This study explores recent research trends and potential applications of ultrasound optical imaging-based multimodal technology. Ultrasound imaging has been widely utilized in medical diagnostics due to its real-time capability and relative safety. However, the drawback of low resolution in ultrasound imaging has prompted active research on multimodal imaging techniques that combine ultrasound with other imaging modalities to enhance diagnostic accuracy. In particular, ultrasound optical imaging-based multimodal technology enables the utilization of each modality's advantages while compensating for their limitations, offering a means to improve the accuracy of the diagnosis. Various forms of multimodal imaging techniques have been proposed, including the fusion of optical coherence tomography, photoacoustic, fluorescence, fluorescence lifetime, and spectral technology with ultrasound. This study investigates recent research trends in ultrasound optical imaging-based multimodal technology, and its potential applications are demonstrated in the biomedical field. The ultrasound optical imaging-based multimodal technology provides insights into the progress of integrating ultrasound and optical technologies, laying the foundation for novel approaches to enhance diagnostic accuracy in the biomedical domain.

Recent advances in optical imaging through deep tissue: Imaging probes and techniques

윤석찬,Seo Young Cheon,Sangjun Park,Donghyun Lee,Yeeun Lee,Seokyoung Han,Moonseok Kim,구희범 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Optical imaging has been essential for scientific observations to date, however its biomedical applications has been restricted due to its poor penetration through tissues. In living tissue, signal attenuation and limited imaging depth caused by the wave distortion occur because of scattering and absorption of light by various molecules including hemoglobin, pigments, and water. To overcome this, methodologies have been proposed in the various fields, which can be mainly categorized into two stategies: developing new imaging probes and optical techniques. For example, imaging probes with long wavelength like NIR-II region are advantageous in tissue penetration. Bioluminescence and chemiluminescence can generate light without excitation, minimizing background signals. Afterglow imaging also has high a signal-to-background ratio because excitation light is off during imaging. Methodologies of adaptive optics (AO) and studies of complex media have been established and have produced various techniques such as direct wavefront sensing to rapidly measure and correct the wave distortion and indirect wavefront sensing involving modal and zonal methods to correct complex aberrations. Matrix-based approaches have been used to correct the high-order optical modes by numerical post-processing without any hardware feedback. These newly developed imaging probes and optical techniques enable successful optical imaging through deep tissue. In this review, we discuss recent advances for multi-scale optical imaging within deep tissue, which can provide reseachers multi-disciplinary understanding and broad perspectives in diverse fields including biophotonics for the purpose of translational medicine and convergence science.

Dual-Modal Nanoprobes for Imaging of Mesenchymal Stem Cell Transplant by MRI and Fluorescence Imaging

성창규,홍경아,Shunmei Lin,이유원,차진명,이진규,홍철표,한봉수,정성일,김승협,윤강섭 대한영상의학회 2009 Korean Journal of Radiology Vol.10 No.6

Objective: To determine the feasibility of labeling human mesenchymal stem cells (hMSCs) with bifunctional nanoparticles and assessing their potential as imaging probes in the monitoring of hMSC transplantation. Materials and Methods: The T1 and T2 relaxivities of the nanoparticles (MNP@SiO2[RITC]-PEG) were measured at 1.5T and 3T magnetic resonance scanner. Using hMSCs and the nanoparticles, labeling efficiency, toxicity, and proliferation were assessed. Confocal laser scanning microscopy and transmission electron microscopy were used to specify the intracellular localization of the endocytosed iron nanoparticles. We also observed in vitro and in vivo visualization of the labeled hMSCs with a 3T MR scanner and optical imaging. Results: MNP@SiO2(RITC)-PEG showed both superparamagnetic and fluorescent properties. The r1 and r2 relaxivity values of the MNP@SiO2(RITC)-PEG were 0.33 and 398 mM-1 s-1 at 1.5T, respectively, and 0.29 and 453 mM-1 s-1 at 3T, respectively. The effective internalization of MNP@SiO2(RITC)-PEG into hMSCs was observed by confocal laser scanning fluorescence microscopy. The transmission electron microscopy images showed that MNP@SiO2(RITC)-PEG was internalized into the cells and mainly resided in the cytoplasm. The viability and proliferation of MNP@SiO2(RITC)-PEG-labeled hMSCs were not significantly different from the control cells. MNP@SiO2(RITC)-PEG-labeled hMSCs were observed in vitro and in vivo with optical and MR imaging. Conclusion: MNP@SiO2(RITC)-PEG can be a useful contrast agent for stem cell imaging, which is suitable for a bimodal detection by MRI and optical imaging. Objective: To determine the feasibility of labeling human mesenchymal stem cells (hMSCs) with bifunctional nanoparticles and assessing their potential as imaging probes in the monitoring of hMSC transplantation. Materials and Methods: The T1 and T2 relaxivities of the nanoparticles (MNP@SiO2[RITC]-PEG) were measured at 1.5T and 3T magnetic resonance scanner. Using hMSCs and the nanoparticles, labeling efficiency, toxicity, and proliferation were assessed. Confocal laser scanning microscopy and transmission electron microscopy were used to specify the intracellular localization of the endocytosed iron nanoparticles. We also observed in vitro and in vivo visualization of the labeled hMSCs with a 3T MR scanner and optical imaging. Results: MNP@SiO2(RITC)-PEG showed both superparamagnetic and fluorescent properties. The r1 and r2 relaxivity values of the MNP@SiO2(RITC)-PEG were 0.33 and 398 mM-1 s-1 at 1.5T, respectively, and 0.29 and 453 mM-1 s-1 at 3T, respectively. The effective internalization of MNP@SiO2(RITC)-PEG into hMSCs was observed by confocal laser scanning fluorescence microscopy. The transmission electron microscopy images showed that MNP@SiO2(RITC)-PEG was internalized into the cells and mainly resided in the cytoplasm. The viability and proliferation of MNP@SiO2(RITC)-PEG-labeled hMSCs were not significantly different from the control cells. MNP@SiO2(RITC)-PEG-labeled hMSCs were observed in vitro and in vivo with optical and MR imaging. Conclusion: MNP@SiO2(RITC)-PEG can be a useful contrast agent for stem cell imaging, which is suitable for a bimodal detection by MRI and optical imaging.

광학적 접근

노재규,김동억 대한의사협회 2004 대한의사협회지 Vol.47 No.2

Optical Imaging Technology for Real-time Tumor Monitoring

Yoo-kyoung Shin,엄주범 대한의학레이저학회 2021 MEDICAL LASERS Vol.10 No.3

Optical imaging modalities with properties of real-time, non-invasive, in vivo, and high resolution for image-guided surgery have been widely studied. In this review, we introduce two optical imaging systems, that could be the core of image-guided surgery and introduce the system configuration, implementation, and operation methods. First, we introduce the optical coherence tomography (OCT) system implemented by our research group. This system is implemented based on a sweptsource, and the system has an axial resolution of 11 μm and a lateral resolution of 22 μm. Second, we introduce a fluorescence imaging system. The fluorescence imaging system was implemented based on the absorption and fluorescence wavelength of indocyanine green (ICG), with a light-emitting diode (LED) light source. To confirm the performance of the two imaging systems, human malignant melanoma cells were injected into BALB/c nude mice to create a xenograft model and using this, OCT images of cancer and pathological slide images were compared. In addition, in a mouse model, an intravenous injection of indocyanine green was used with a fluorescence imaging system to detect real-time images moving along blood vessels and to detect sentinel lymph nodes, which could be very important for cancer staging. Finally, polarization-sensitive OCT to find the boundaries of cancer in real-time and real-time image-guided surgery using a developed contrast agent and fluorescence imaging system were introduced.

광영상의 이비인후과 영역에서 임상적 적용

이상준 대한이비인후과학회 2015 대한이비인후과학회지 두경부외과학 Vol.58 No.4

Optical imaging visualizes tissue function and structure by analyzing the properties of absorption, scattering, or reflection. Light in the near-infrared spectrum relatively penetrate human tissue well. Diffuse optical imaging (DOI) is a functional imaging modality which can evaluate the perfusion and metabolism of human tissue and tumor by analyzing the optical properties of hemoglobin, water, and lipid. Optical coherence tomography (OCT) acquires the cross-sectional images by analyzing the coherence pattern of the reflected light from the human tissue. OCT has higher resolution more than 15 times compared to conventional imaging modalities like ultrasonography, computed tomography, or magnetic resonance imaging. Optical imaging has advantages of harmlessness, noninvasiveness, and high resolution. However, it has limitation in the penetration depth. In this review, mechanism of DOI and OCT and their clinical application in the otorhinolaryngology field will be discussed.

Quality assessment of the optical thin films using line field spectral domain optical coherence tomography

Shirazi, Muhammad Faizan,Wijesinghe, Ruchire Eranga,Ravichandran, Naresh Kumar,Kim, Pilun,Jeon, Mansik,Kim, Jeehyun Elsevier 2018 Optics and lasers in engineering Vol.110 No.-

<P>In this study, the industrial inspection of optical thin film on touch screen panels was demonstrated using line field spectral domain optical coherence tomography. The conventional Fourier domain optical coherence tomography system requires a single scanner for two-dimensional cross-sectional images and two scanners for volumetric images. Our developed line field spectral domain optical coherence tomography has the advantage of needing only a single scanner for volumetric images, while two-dimensional cross-sectional images are obtained by the parallel acquisition of an illuminated line on a sample using an area camera. Further, the image acquisition speed was enhanced by implementing a high speed camera (340 frames per seconds) with improved quantum efficiency at near infrared region enabling two-fold frame rate. Cross-sectional and volumetric images were acquired to detect the internal sublayer defects in the optical thin films, which are difficult to observe using visual or machine vision-based inspection methods. The developed pseudo code for defect identification in optical thin films was well-utilized here for the defect inspection. The system characterization is demonstrated using United State Air Force (USAF) resolution target. The results indicate the possible application of the proposed system in touch screen panel inspection for the quality assurance of products at the consumer end.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Quality assessment of the optical thin films used for touch screen panel. </LI> <LI> Line illumination is utilized to get the cross-sectional information without scanner. </LI> <LI> The sublayer defects are detected using line field optical coherence tomography. </LI> <LI> A defect detection algorithm is developed for the quality assurance of optical thin films. </LI> <LI> Cross-sectional and three-dimensional images show the effectiveness of the system. </LI> </UL> </P>

기계적 주사장치가 없이 측면 광학 영상 구현이 가능한 광프로브

강희원,이승석,최은서 한국물리학회 2019 새물리 Vol.69 No.9

In this paper, we introduce optical probes that can acquire side-view optical images at one time without using a mechanical scanning device. In the conventional optical image acquisition method, the light beam is rotated by using a mechanical scanning device. However, in that method, not only does the image acquisition take a long time but also the synchronization of the operational speed between the mechanical rotation and the image acquisition may not be properly performed, thus causing a problem in detecting an accurate optical image. To solve this problem, we propose an optical probe capable of transmitting light to a cylindrical sample and receiving reflected light by using a cone-shaped reflecting mirror. We confirmed the validity of the proposed side-view optical imaging probe, by acquiring both images of the pattern and the characters on the inner surface of a cylindrical sample and a fluorescent image of a surface overlaid with fluorescent beads. 본 논문에서는 기계적인 스캐닝 장치를 이용하지 않고 측면 광학 영상을 한 번에 획득할 수 있는 광프로브를 소개하고자 한다. 기존 광학 영상 획득 방법에서는 기계적인 스캐닝 장치를 이용하여 조사되는빔을 회전시켜 측면 영상을 획득하였다. 하지만 이러한 방법의 경우 영상 획득의 시간이 오래 걸릴 뿐만아니라 기계적인 회전과 영상 획득에서의 동작 속도의 동기화가 원활하게 이루어지지 않아 정확한 광학영상을 구현하는데 문제점을 가지고 있다. 이러한 문제점을 해결하기 위해 원뿔형의 반사거울을 이용하여원통형의 샘플 내부로 광을 전달하고 반사된 광을 수광할 수 있는 광프로브를 제안하고자 한다. 제안한측면 광학 영상 프로브를 이용하여 원통형 샘플의 내부 표면에 쓰인 패턴과 글자 영상과 형광비드가 도포된표면의 형광 영상을 획득함으로써 제안한 방법의 가능성을 확인해보고자 하였다.

Application of optical small animal imaging in preclinical research

Joon-Suk Park 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Molecular imaging can be used as a non-invasive means to evaluate pathophysiological processes, such as cancer, in rodents. Indeed, these techniques provide real-time information for early diagnosis, allow longitudinal follow-up of tumor development, and facilitate studies of therapeutic activity and antitumor efficacy of new anti-cancer drugs. Molecular imaging of tumors in preclinical models is of the utmost importance for developing innovative cancer treatments. This field is moving extremely rapidly, with recent advances in optical imaging technologies and sophisticated molecular probes for in vivo imaging. In vivo optical imaging is a noninvasive technique that utilizes bioluminescent and fluorescent endogenous reporters or exogenous probes to repetitively interrogate molecular events and monitor disease progression in animal models. This presentation includes an overview of optical imaging technologies (e.g., hardware, reporters, probes) available for small animal imaging and their application in monitoring disease progression, therapeutic efficacy, and molecular processes such as proliferation, apoptosis, and angiogenesis. The optical small animal imaging technologies and applications described above will accelerate both academic and pharmaceutical preclinical research across multiple therapeutic and drug discovery areas, and provide valuable molecular information to bridge and inform clinical trials.

Optical Coherence Tomography-Piezoelectric Transducer Probe를 이용한 치과 영역 광단층영상기법 적용

김상우,최은서,이승석,임원봉,김지선,전상미,최홍란,김병국,차현록,임대영,임순임,김창수,김옥준 대한구강악안면병리학회 2013 대한구강악안면병리학회지 Vol.37 No.2

We demonstrate optical cross-sectional imaging system implemented with high-resolution interferometry and present oral diagnostic imaging results obtained without any physical sectioning. High-resolution interferometry could be performed with utilizing broadband optical source and employment of beam scanning device to high-resolution interferometer constitutes optical imaging system for non-invasive cross-sectional view at real-time. The optical imaging system is implemented with fiber-optic devices for compactness and optical probe head is realized by using single mode optical fiber and miniaturized actuator, which is properly designed for the application to dental imaging. The basic performance of the optical imaging system, for example, such as resolution, imaging depth, and sensitivity is suggested to prove high-resolution optical imaging performance. Feasibility of the developed optical imaging system performance in the application of dental diagnostic is proved with demonstrating non-invasively obtained cross-sectional images. The imaging quality suggested in the images could be applied to assessment of oral diseases and used for alternative imaging modality to X-ray diagnostic method overcoming disadvantage of low-image resolution. The imaging performance enabling real-time image reconstruction also could be exploited as early oral diagnostic apparatus prior to microscopic observation under H&E staining.