New bis-thioglycosyl-1,1′-disulfides from <i>Nasturtium officinale</i> R. Br. and their anti-neuroinflammatory effect

Lee, Tae Hyun,Khan, Zahra,Subedi, Lalita,Kim, Sun Yeou,Lee, Kang Ro Elsevier 2019 Bioorganic chemistry Vol.86 No.-

<P><B>Abstract</B></P> <P>As a part of our continuing search for bioactive constituents from Brassicaceae family, three new bis-thioglycosides (<B>1</B>–<B>3</B>) were isolated from the 80% MeOH extract of <I>Nasturtium officinale</I>, together with 13 known compounds (<B>4</B>–<B>16</B>). The chemical structures of three new bis-thioglycosides (<B>1</B>–<B>3</B>) were elucidated using NMR techniques (<SUP>1</SUP>H and <SUP>13</SUP>C NMR, <SUP>1</SUP>H–<SUP>1</SUP>H COSY, HSQC, and HMBC), HRESIMS, and a chemical method. All the compounds were evaluated for their inhibitory effects on nitric oxide (NO) levels in lipopolysaccharide (LPS)-stimulated murine microglia BV-2 cells. Among the isolates, compound <B>5</B> exhibited a strong inhibitory effect on NO production, and compounds <B>4</B> and <B>15</B> showed moderate inhibitory activities, suggesting the neuroprotective and anti-neuroinflammatory effects of bis-thioglycosides from <I>N. officinale</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three new bis-thioglycosyl-1,1′-disulfides were isolated from <I>Nasturtium officinale</I>. </LI> <LI> The chemical constituents of the isolates were elucidated using NMR and MS data. </LI> <LI> Compound <B>5</B> exhibited significant inhibition activity of NO production with IC<SUB>50</SUB> value of 21.30 µM. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Antioxidant Efficacy of Nasturtium officinale Extracts Using Various In Vitro Assay Systems

Seifollah Bahramikia,Razieh Yazdanparast 사단법인약침학회 2010 Journal of Acupuncture & Meridian Studies Vol.3 No.4

Nasturtium officinale R. Br. (watercress), of the family Brassicaceae, has been long used as a home remedy or a medicinal plant by the people of southeastern Iran. The aim of this study was to investigate the antioxidant activity of N. officinale extract using various in vitro assay systems, including the ferric reducing antioxidant power and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonate) assays, 1,1-diphenyl-2-picrylhydrazyl, hydrogen peroxide, nitric oxide radical scavenging, and ferrous ion chelating activity, as well as the inhibitory effect on ferrous ion/ascorbate induced lipid peroxidation, in rat liver homogenate. The results revealed that N. officinale extract possesses potent reducing power in a ferric reducing antioxidant power assay, concentration-dependent scavenging ability on 2,2-azinobis 3-ethylbenzothiazoline-6-sulfonate, 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals,and hydrogen peroxide, as well as chelating ability on ferrous ions. Furthermore,N. officinale extract prevented thiobarbituric acid reactive substances formation in ferrous ion/ascorbate induced lipid peroxidation in rat liver homogenate in a dosedependent manner. In addition, this N. officinale extract had the phenolic and flavonoid contents of 96.2 mg gallic acid equivalents/g dried extract and 63.2 mg catechin equivalents/g dried extract, respectively. The cumulative results clearly indicate that N. officinale extract possesses potent antioxidant properties probably mediated through direct trapping of free radicals, reducing power, and also through metal chelating. Based on its antioxidative potential, N. officinale extract might find applications in the prevention of free radical-related diseases.

배양액의 종류 및 광질이 물냉이의 생육 및 Glucosinolate 함량에 미치는 영향

최재윤(Jae Yun Choi),김성진(Sung Jin Kim),복권정(Kwon Jeong Bok),이광야(Kwang Ya Lee),박종석(Jong Seok Park) (사)한국생물환경조절학회 2018 생물환경조절학회지 Vol.27 No.4

배양액의 종류 및 LED를 이용한 다양한 광질 조건이 물냉이의 생장과 glucosinolates 함량에 미치는 영향을 3주간의 수경재배를 통하여 살펴보았다. 물냉이 종자를 암면배지에 파종 후 발아시켜, 2주간 육묘하였다. 유묘들은 semi-DFT 시스템에 이식하였다. 환경조절실은 주간온도 20±1oC와 야간온도 16±1℃, 주간습도 65±10%와 야간습도 75±10%의 범위에서 조절되었으며 16/8h 조건의 광주기와 180±10μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP> 광강도를 유지하였다. 배양액은 오오츠카 하우스 1A(OTS), 한국원시(HES)과 화란온실작물연구소(PBG) 배양액을 초기 EC 1.0-1.3, pH 6.2와 형광등을 광원으로 실험하였다(실험-1). 광질에 대한 생육과 glucosinolates 함량을 분석하기 위하여 단색광(적색: R10, 백색: W10) 처리구와 혼합광(적청녹색: R2B1G1, 백청녹색: W2B1G1, 적색: R10, 적청색: R5B1, 적청색: R3B1)처리구를 두었다. 물냉이 지상부의 생육은 3개의 배양액 처리구에서 유의적인 차이가 발견되지 않았지만, 뿌리의 생체중은 HES와 비교하여 PBG와 OTS에서 13.7%와 55.1% 증가하였다. OTS 처리는 PBG와 HES 처리구에 비해 gluconasturtiin 함량이 96%, 65% 증가하였다. 백색광조건(W10)과 비교하여 적색광(R10) 처리는 초장이 101.3% 증가하였다. 청색광 비율의 증가는 지상부 생육에 긍정적인 영향을 주었다. 물냉이의 건물중 당 glucosinolates 함량은 R2B1G1 처리구와 비교하여 R3B1 처리구에서 144.5% 증가하였으며, W10 처리구와 비교 시, 약 70% 증가하는 경향을 보였다. R3B1 처리구에서 물냉이의 생육과 총 glucosinolates의 함량이 가장 높게 나타났다. Aim of this study was to investigate the effects of different nutrient solutions and various light qualities generated by LED on the growth and glucosinolates contents of watercress (Nasturtium officinale) grown under hydroponics for 3 weeks. The seeds of watercress were sown on crushed rockwool media and raised them for two weeks. They were transplanted in a semi-DFT (deep flow technique) hydroponics system. A controlled-environment room was maintained at 20±1℃ and 16±1℃ temperatures and 65±10% and 75±10% relative humidity (day and night, respectively), with a provided photosynthetic photon flux density (PPFD) of 180±10 μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP> and a photoperiod of 16/8h. To find out the best kinds of nutrient solutions for growing watercress, Otsuka House 1A (OTS), Horticultural Experiment Station in Korea (HES), and Netherland’s Proefstaion voor Bloemisterij en Gasgroente (PBG) were adapted with initial EC of 1.0-1.3 dS·m<SUP>-1</SUP> and pH of 6.2, irradiating PPFD with fluorescent lamps (Ex-1). Either monochromatic (W10 and R10) or mixed LEDs (R5B1, R3B1, R2B1G1, and W2B1G1) were irradiated with a differing ratio of each LED’s PPFD to understanding light quality on the growth and glucosinolates contents of watercress (Ex-2). Although significant difference in the shoot growth of watercress was not found among three nutrient solutions treatments, but the root fresh weight increased by 13.7% and 55.1% in PBG and OTS compared to HES, respectively. OTS increased the gluconasturtiin content by 96% and 65% compared to PBG and HES. Compared with the white light (W10), the red light (R10) showed a 101.3% increase in the shoot length of watercress. Increasing blue light portion positively affected plant growth. The content of total glucosinolates in watercress was increased by 144.5% and 70% per unit dry weight in R3B1 treatment compared with R2B1G1 and W10 treatments, respectively. The growth and total glucosinolates contents of the watercress were highest under R3B1 among six light qualities.

Biological responses of a non-target aquatic plant (Nasturtium officinale) to the herbicide, tribenuron-methyl

Duman, Fatih,Urey, Erkan,Temizgul, Ridvan,Bozok, Fuat The Korean Society of Weed Science and The Turfgra 2010 Weed Biology and Management Vol.10 No.2

To assess its response to the herbicide, tribenuron-methyl, samples of Nasturtium officinale were exposed to 0, 0.01, 0.05, 0.1, and 0.5 $mg\;L^{-1}$ of tribenuron-methyl for 1, 2, 4 and 7 days. The influence of this herbicide on the relative growth rate, electrolyte leakage, lipid peroxidation, photosynthetic pigmentation, protein content, and performance of anti-oxidant enzymes, such as superoxide dismutase (SOD), catalase, and ascorbate peroxidase (APX), was examined. The results indicated that tribenuron-methyl, applied at 0.5 $mg\;L^{-1}$, affected plant growth negatively. It also was determined that chlorophyll a is the most responsive photosynthetic pigment to tribenuron-methyl exposure. Under stress conditions, the anti-oxidant enzymes were up-regulated compared to the control. The SOD activity was significantly stimulated, while the activity of APX was inhibited. A significant correlation was found between lipid peroxidation and SOD activity. The exposure period and herbicide concentration had significant effects on the biological responses against tribenuron-methyl stress. These results may be useful for clarifying the effect of herbicides on non-target aquatic plants.

The Effect of Temperature, Photoperiod, and Light Quality on Gluconasturtiin Levels in Watercress (Nasturtium officinale R. Br.)

Gerard Engelen-Eigles,Greg Holden,Jerry D. Cohen,Gary Gardner 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-