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- 주제어 : Myogenic stem cell (검색결과 9 건)
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Song, Minjung,Kim, Hana,Choi, Yoonjeong,Kim, Kyungho,Chung, Chooryung The Korean Association Of Orthodontists 2012 대한치과교정학회지 Vol.42 No.5
Objective: To investigate the stem cell-like characteristics of human periodontal ligament (PDL) stromal cells outgrown from orthodontically extracted premolars and to evaluate the potential for myogenic differentiation. Methods: PDL stromal cells were obtained from extracted premolars by using the outgrowth method. Cell morphological features, self-replication capability, and the presence of cell-surface markers, along with osteogenic, adipogenic, and chondrogenic differentiation, were confirmed. In addition, myogenic differentiation was induced by the use of 5-aza-2'-deoxycytidine (5-Aza) for DNA demethylation. Results: PDL stromal cells showed growth patterns and morphological features similar to those of fibroblasts. In contrast, the proliferation rates of premolar PDL stromal cells were similar to those of bone marrow and adipogenic stem cells. PDL stromal cells expressed surface markers of human mesenchymal stem cells (i.e., CD90 and CD105), but not those of hematopoietic stem cells (i.e., CD31 and CD34). PDL stromal cells were differentiated into osteogenic, adipogenic, and chondrogenic lineages. Myotube structures were induced in PDL stromal cells after 5-Aza pretreatment, but not in the absence of 5-Aza pretreatment. Conclusions: PDL stromal cells isolated from extracted premolars can potentially be a good source of postnatal stem cells for oromaxillofacial regeneration in bone and muscle.
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송민정,김하나,최윤정,김경호,정주령 대한치과교정학회 2012 대한치과교정학회지 Vol.42 No.5
Objective: To investigate the stem cell-like characteristics of human periodontal ligament (PDL) stromal cells outgrown from orthodontically extracted premolars and to evaluate the potential for myogenic differentiation. Methods: PDL stromal cells were obtained from extracted premolars by using the outgrowth method. Cell morphological features, self-replication capability, and the presence of cell-surface markers, along with osteogenic, adipogenic, and chondrogenic differentiation, were confirmed. In addition, myogenic differentiation was induced by the use of 5-aza-2’-deoxycytidine (5-Aza) for DNA demethylation. Results: PDL stromal cells showed growth patterns and morphological features similar to those of fibroblasts. In contrast, the proliferation rates of premolar PDL stromal cells were similar to those of bone marrow and adipogenic stem cells. PDL stromal cells expressed surface markers of human mesenchymal stem cells (i.e., CD90 and CD105), but not those of hematopoietic stem cells (i.e., CD31 and CD34). PDL stromal cells were differentiated into osteogenic, adipogenic, and chondrogenic lineages. Myotube structures were induced in PDL stromal cells after 5-Aza pretreatment, but not in the absence of 5-Aza pretreatment. Conclusions: PDL stromal cells isolated from extracted premolars can potentially be a good source of postnatal stem cells for oromaxillofacial regeneration in bone and muscle.
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Minjung Song,Hana Kim,Yoonjeong Choi,Kyungho Kim,Chooryung Chung 대한치과교정학회 2012 대한치과교정학회지 Vol.42 No.5
Objective: To investigate the stem cell-like characteristics of human periodontal ligament (PDL) stromal cells outgrown from orthodontically extracted premolars and to evaluate the potential for myogenic differentiation. Methods: PDL stromal cells were obtained from extracted premolars by using the outgrowth method. Cell morphological features, self-replication capability, and the presence of cell-surface markers, along with osteogenic, adipogenic, and chondrogenic differentiation, were confirmed. In addition, myogenic differentiation was induced by the use of 5-aza-2’-deoxycytidine (5-Aza) for DNA demethylation. Results: PDL stromal cells showed growth patterns and morphological features similar to those of fibroblasts. In contrast, the proliferation rates of premolar PDL stromal cells were similar to those of bone marrow and adipogenic stem cells. PDL stromal cells expressed surface markers of human mesenchymal stem cells (i.e., CD90 and CD105), but not those of hematopoietic stem cells (i.e., CD31 and CD34). PDL stromal cells were differentiated into osteogenic, adipogenic, and chondrogenic lineages. Myotube structures were induced in PDL stromal cells after 5-Aza pretreatment, but not in the absence of 5-Aza pretreatment. Conclusions: PDL stromal cells isolated from extracted premolars can potentially be a good source of postnatal stem cells for oromaxillofacial regeneration in bone and muscle.
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Vahid Bayati,Mahmoud Hashemitabar,Roohollah Gazor,Reza Nejatbakhsh,Dariush Bijannejad 대한해부학회 2013 Anatomy & Cell Biology Vol.46 No.2
In recent years, examination and comparison of the biological characteristics of bone marrow- and adipose-derived mesenchymal stem cells (MSCs) from various perspectives have come into the focus of stem cell research, as these cells should be well characterized in order to utilize them in future cellular therapies. Therefore, in the present study, surface protein markers and the skeletal myogenic differentiation potential of rat bone marrow- and adipose-derived MSCs were examined. The expression of CD44, CD45, CD73, and CD90 on bone marrow- and adipose-derived MSCs was characterized using flow cytometry. Subsequently, the stem cells were differentiated into myogenic lineages, and the expression of the skeletal myogenic markers MyoD1, Myog, and Myh2 was studied in cells using real time polymerase chain reaction and immunofluorescence. Our results reveal that the pattern of CD marker expression differs between these 2 types of MSCs to some extent, whereas no significant difference was observed with respect to their myogenic differentiation potential. Therefore, we concluded that despite the differences observed in the biological features of these 2 types of MSCs, their myogenic potential appears to be similar, and that adipose-derived stem cells may be useful in skeletal muscle tissue engineering, due to their easy isolation and capacity for rapid expansion in a short time span.
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유승미,김현범,강미경,황석연,김병수 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
Cells in living tissues are engaged in complex cell-cell interaction with heterogeneous cells in the face of a dynamically changing in vivo environment. In order to recapitulate the complex microenvironment, various cellular assembly approaches, mostly rely on irreversible cell layering, have been proposed. Inefficient interactions among cells in the techniques prevent characterization and therapeutic applications of the cells following co-culture. Here, we develop a reversible cell layering system for heterogeneous cell assembly mediated by ionic cross-linking of chitosan and a functionalized cell surface membrane. Anionic maleimide-chondroitin-sulfate is grafted onto the surface membrane of myogenic cells and human mesenchymal stem cells (hMSCs) via ionic cross-linking forming assembled double-layered cell constructs. hMSCs differentiated through this system showed higher levels of myogenic marker expression during muscle regeneration.
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Myogenic Differentiation of Human Adipose-Derived Stem Cells
박윤길,최정화,Ah Mi Baek,도병록,Sun Do Kim 대한재활의학회 2011 Annals of Rehabilitation Medicine Vol.35 No.1
Objective Cell therapy has been extensively studied as a gene complementation approach in muscular dystrophy including Duchenne muscular dystrophy (DMD), and adipose tissue has recently been identified as a uniquely abundant and adequately accessible source of pluripotent cells. In the present work, we investigated myogenic potentials of adipose-derived stem cells (ADSCs) depending on culture media and isolation with using surface markers. Method Human ADSCs were obtained by liposuction and cultured in two different media; control and myogenic media. In addition we attempted to isolate ADSCs by utilizing surface markers: CD45 and CD133. The following observations were made to evaluate myogenic differentiation as the expression of myogenic regulatory factors (MyoD, Myf-5 and Myf-6) and desmin by RT-PCR and immunoflurescence study. Results Conversion of ADSCs to myogenic phenotype was observed by indirect immunoflurescence study of MyoD and Myf-5 in regardless of media type and isolation method. In addition mRNA of MyoD and Myf-5 were positive in both culture media, and there were no differences of MyoD and Myf-5 responses between CD45− and CD45−CD133− ADSCs. However, secondary myogenic regulatory factor (Myf-6) was not expressed constantly, and desmin were negative in all cultural condition. Conclusion Our findings suggest that human ADSCs might have myogenic potentials. However, further studies are needed to express the secondary myogenic regulatory factors and proteins in myoblasts.
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( Kil Hwan Kim ),( Mi Ae Lee ),( Byung Hyune Choi ),( So Ra Park ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.1
Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types, including chondrocytes, osteocytes, adipocytes, myocytes, and neurons. However, more efficient methods for specific lineage differentiation from MSCs are still required for their clinical application. In the present work we examined the effects of low intensity ultrasound (LIUS) on myogenic and adipogenic differentiation of MSCs isolated from the bone marrow of rats. We found that the LIUS stimulation has inhibitory effects on both differentiation toward myocyte and adipocyte from rat MSCs (rMSCs). bFGF enhanced the myogenic and adipogenic differentiation when MSCs were cultured in each differentiation medium, inducing the mRNA expression of myogenic and adipogenic markers. The LIUS stimulation showed the decrease in the expression of myogenic markers, desmin and troponin C, in the condition of myogenic differentiation containing bFGF. Similarly, the LIUS stimulation also showed the decrease in the expression of PPAR and the Oil Red O staining in adipogenic differentiation. These results reveal that mechanical stimulation by LIUS attenuates both myogenic and adipogenic differentiation of rMSCs in vitro.
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Lee, E.J.,Bajracharya, P.,Jang, E.J.,Chang, J.S.,Lee, H.J.,Hong, S.K.,Choi, I. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.5
Myogenic satellite cells (MSCs) are adult stem cells that activate and differentiate into myotubes. These stem cells are multipotent as they transdifferentiate into adipocyte-like cells, nerve cells and osteocytes. The effects of steroid hormones ($E_2$ and testosterone) were studied as a further step toward understanding the mechanism of MSCs proliferation and differentiation. In this study, MSCs were grown continuously for 87 days, implying that there may be a group of MSCs that continue to proliferate rather than undergoing differentiation. Isolated MSCs were cultured in Dulbecco's Modified Eagle's Medium supplemented with adult male, female or castrated bovine serum to observe the effect of steroid hormones on MSC proliferation. Cell proliferation was the highest in cultures supplemented with male serum followed by female and castrated serum. The positive effect of male hormone on MSC proliferation was confirmed by the observation of testosterone-mediated increased proliferation of cells cultured in medium supplemented with castrated serum. Furthermore, steroid hormone treatment of MSCs increased lipid accumulation in myotubes. Oil-Red-O staining showed that 17${\beta}$-estradiol ($E_2$) treatment avidly increased lipid accumulation, followed by $E_2$+testosterone and testosterone alone. To our knowledge, this is the first report of lipid accumulation in myotubes due to steroids in the absence of an adipogenic environment, and the effect of steroid hormones on cell proliferation using different types of adult bovine serum, a natural hormonal system. In conclusion, we found that sex steroids affect MSCs proliferation and differentiation, and lipid accumulation in myotubes.
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