Influences of Animal Mucins on Peroxidase Activity in Solution and on the Surface of Hydroxyapatite

이상구,전은형,고홍섭,Lee, Sang-Goo,Jeon, Eun-Hyoung,Kho, Hong-Seop Korean Academy of Orofacial Pain and Oral Medicine 2008 Journal of Oral Medicine and Pain Vol.33 No.3

Animal mucins have structural characteristics similar to human salivary mucins. Animal mucins have been regarded as suitable substances for saliva substitutes. Since animal mucin molecules in saliva substitutes and host-derived antimicrobial salivary molecules exist simultaneously in whole saliva and the pellicles of patients with dry mouth, interactions may occur between these molecules. The purpose of this study was to investigate the influence of animal mucins on peroxidase activity in solution and on the surface of hydroxyapatite(HA) surfaces. The effects of animal mucins on peroxidase activity were examined by incubating porcine gastric mucin(PGM) or bovine submaxillary mucin (BSM) with either bovine lactoperoxidase(bLPO) or saliva samples. For solid-phase assays, immobilized animal mucins or peroxidase on three different HA surfaces(HA beads, HA disc, and bovine tooth) were used. Peroxidase activity was determined with an NbsSCN assay. The obtained results were as follows: 1. PGM enhanced the enzymatic activity of bLPO in solution phase. PGM did not affect the enzymatic activity of peroxidase in saliva sample(POS). 2. BSM did not affect the enzymatic activities of both bLPO and POS in solution phase. 3. HA-adsorbed PGM increased subsequent bLPO adsorption in all three HA phases. The activity of POS was increased on both the HA beads and bovine tooth. 4. The peroxidase activities on the HA beads and disc were increased when the HA surfaces were exposed to a mixture of bLPO and PGM. 5. The binding affinity of bLPO to PGM was greater than that of bLPO to BSM. Collectively, our results suggest that animal mucins affects the enzymatic activity of peroxidase on the HA surfaces as well as in solution. Saliva substitutes containing animal mucins may affect the function of antimicrobial components in natural saliva and saliva substitutes.

Mucinous precursor lesions of mucinous carcinoma in breast: Incidence and histopathologic features

정민정,김영옥 고신대학교(의대) 고신대학교 의과대학 학술지 2015 고신대학교 의과대학 학술지 Vol.30 No.2

Objectives: Columnar cell lesion (CCL), atypical ductal hyperplasia (ADH) and ductal carcinoma in situ (DCIS) may be premalignant lesion of mammary invasive carcinoma. A few recent investigators reported that the precursor lesions exhibited mucin production and they might be potential precursor lesion for mucinous carcinoma (mCA). This study aims to investigate the incidence and histopathologic characteristics of mucinous precursor lesions, including mucinous DCIS (mDCIS) and mucinous CCL (mCCL). Methods: We retrospectively analyzed invasive carcinomas with mucin. Cases were grouped into three subgroups: pure mCA, mixed mCA, and invasive carcinoma of no special type with mucin production (IC of NST-m). Precursor lesions were evaluated with PAS and alcian blue staining. Results: Total 27 cases of invasive carcinoma with mucin were analysed and classified as 18 pure mCA, 7 mixed mCA, and 2 IC of NST-m. mDCISs were found in 12 pure mCA, 4 mixed mCA and 2 IC of NST-m. mCCLs were found in 7 pure mCA and 2 mixed mCA. Majority of mucin was identified in both cytoplasm and ductal lumen, while some tumors exhibited only cytoplasmic mucin. We also observed three patterns of mDCIS classifiable by location of mucin and architecture of tumor cells. Conclusions: Cytoplasmic mucin suggested that mucinous feature of precursor lesions in the vicinity of mCA might not be a passive morphologic finding but be involved in development of mCA.

Spectrum of mucin-producing neoplastic conditions of the abdomen and pelvis: cross-sectional imaging evaluation.

Lee, Nam Kyung,Kim, Suk,Kim, Hyun Sung,Jeon, Tae Yong,Kim, Gwang Ha,Kim, Dong Uk,Park, Do Youn,Kim, Tae Un,Kang, Dae Hwan WJG Press 2011 WORLD JOURNAL OF GASTROENTEROLOGY Vol.17 No.43

<P>Various mucin-producing neoplasms originate in different abdominal and pelvic organs. Mucinous neoplasms differ from non-mucinous neoplasms because of the differences in clinical outcome and imaging appearance. Mucinous carcinoma, in which at least 50% of the tumor is composed of large pools of extracellular mucin and columns of malignant cells, is associated with a worse prognosis. Signet ring cell carcinoma is characterized by large intracytoplasmic mucin vacuoles that expand in the malignant cells with the nucleus displaced to the periphery. Its prognosis is also generally poor. In contrast, intraductal papillary mucinous neoplasm of the bile duct and pancreas, which is characterized by proliferation of ductal epithelium and variable mucin production, has a better prognosis than other malignancies in the pancreaticobiliary tree. Imaging modalities play a critical role in differentiating mucinous from non-mucinous neoplasms. Due to high water content, mucin has a similar appearance to water on ultrasound (US), computed tomography (CT), and magnetic resonance imaging, except when thick and proteinaceous, and then it tends to be hypoechoic with fine internal echoes or have complex echogenicity on US, hyperdense on CT, and hyperintense on T1- and hypointense on T2-weighted images, compared to water. Therefore, knowledge of characteristic mucin imaging features is helpful to diagnose various mucin-producing neoplastic conditions and to facilitate appropriate treatment.</P>

췌장 점액낭성종양 41예의 임상 및 조직 소견

김종철 ( Jong Cheol Kim ),김명환 ( Myung Hwan Kim ),이태윤 ( Tae Yoon Lee ),김지영 ( Ji Young Kim ),한정혜 ( Jeung Hye Han ),박수정 ( Soo Jung Park ),이상수 ( Sang Soo Lee ),서동완 ( Dong Wan Seo ),장세진 ( Sei Jin Jang ),이성구 ( 대한소화기학회 2008 대한소화기학회지 Vol.51 No.1

목적: 점액낭성종양은 최근 진단 예가 증가되고 있다. 이번 연구에서는 점액낭성종양의 임상 특징들을 알아보고, 또한 비침습 낭성종양과 침습 낭성종양의 임상 차이 및 수술 후 경과와 예후에 관해서 알아보고자 하였다. 대상 및 방법: 1995년 1월부터 2004년 12월까지 서울아산병원에서 췌장의 점액낭성종양으로 수술을 시행 받은 41예를 대상으로 환자의 증상, 수술방법, 병리, 검사실 소견, 장기생존율 등을 후향적인 방법으로 조사하였다. 결과: 병리 소견으로는 총 41예 중 선종이 33예, 경계 병변이 1예, carcinoma in situ가 1예, 침습 종양이 6예였다. 여자가 남자보다 약 4배 많았으며 평균연령은 49세였다. 우연히 발견된 경우가 가장 많았고 증상으로는 복통이 16예로 가장 많았다. 종양의 가장 흔한 위치는 미부였다. 침습 종양과 비침습 종양 간에 의미 있는 차이를 보인 인자는 복통, 종양의 크기와 벽재결절이었다. 치료성적은 비침습 종양은 수술 후 추적관찰 기간 동안 모두 생존하였고 침습 종양에서는 6예 중 2예에서 사망하였다. 결론: 점액낭성종양에서 침습 종양을 시사하는 임상 인자는 크기와 복통이다. 비침습 종양은 수술 후 예후가 매우 좋으므로 적극적인 치료가 요구되며 평균 42개월 추적 결과 재발예가 없었다. Background/Aims: Intraductal papillary mucinous neoplasms (IPMN) and mucinous cystic neoplasms are included in mucin-producing pancreatic tumors. The reports about IPMN are not uncommon but those about the mucinous cystic neoplasms are relatively few. The aims of this study were to define the natural history of resected mucinous cystic neoplasms of the pancreas and to identify the findings which suggest malignancy. Methods: The authors retrospectively evaluated the clinical outcomes of 41 patients with mucinous cystic neoplasms who were surgically resected at Asan Medical Center between 1995 and 2004. Results: Women (n=33) were more frequently affected than men (n=8). Thirty three patients (80.6%) had adenoma, 1 (2.4%) borderline malignancy, 1 (2.4%) carcinoma in situ, and 6 (14.6%) invasive mucinous cystadenocarcinoma. The most frequent symptom was abdominal pain (39%). About half of the enrolled patients were asymptomatic. Unilocular type (79%) was more frequent than the multilocular type (21%) on gross morphology. The tumor size of invasive mucinous cystic neopolasms was larger than that of non-invasive mucinous cystic neoplalsms (p=0.01). Abdominal pain was more frequent in invasive mucinous cystic neoplasms (p=0.026). On gross morphology, mural nodules were detected in 4 of 6 patients with invasive mucinous cystic neoplasms. However, they were not detected in any patients with non-invasive mucinous cystic neoplasms. Recurrence developed in none of the 35 patients with non-invasive mucinous cystic neoplasms, however 2 of the 6 patients with invasive mucinous cystic neoplasms died within 5 years. Conclusions: Clinical predictors of invasive mucinous cystic neoplasms are suggested to be tumor size and abdominal pain. The prognosis of the non-invasive mucinous cystic neoplasms is excellent when curative resection is performed. (Korean J Gastroenterol 2008;51:34-39)

결장 및 직장 점액암의 임상 양상

안창혁,강원경,박승철,홍민광,이도상,오승택,전해명 대한대장항문학회 2001 Annals of Coloproctolgy Vol.17 No.2

특징적 임상 양상 및 예후를 가지는 결장 및 직장의 점액성 선암

유윤식,김희철,박상준,유장학,김정선,이강홍,유창식,김진천 대한대장항문학회 2003 Annals of Coloproctolgy Vol.19 No.6

가감정기탕(加減正氣湯), 가미화정전(加味和正煎), 가미통규탕(加味通竅湯)이 기도점액 분비에 미치는 영향

한재경,김윤희,채호연,Han, Jae-Kyung,Kim, Yun-Hee,Chae, Ho-Youn 대한한방소아과학회 2007 대한한방소아과학회지 Vol.21 No.1

Objectives : In the present study, the author intended to investigate Gagam-jeonggitang(GJT), Gami-hwajeongjeon(GHJ) and Gami-tonggyutang(GTT) significantly affect in vivo and in vitro mucin secretion from airway epithelial cells. Methods : In vivo experiment, the author induced hypersecretion of airway mucin, hyperplasia of tracheal goblet cells and the increase in intraepithelial mucosubstances by exposing rats to SO2 during 3 weeks. Effects of orally-administered GJT, GHJ and GTT during 1 week on in vivo mucin secretion and hyperplasia of tracheal goblet cells were assesed using ELISA and staining goblet cells with alcian blue. For in vitro experiment, confluent HTSE cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of each agent to assess the effects of each agent on 3H-mucin secretion. Possible cytotoxicities of each agent were assessed by measuring lactate dehydrogenase release. Also, the effects of each agent on contractility of isolated tracheal smooth muscle and effects of each agent on MUC5AC gene expression in cultured HTSE cells were investigated. Results : GJT, GHJ and GTI inhibited hypersecretion of in vivo mucin: GJT and GHJ inhibited the increase of number of goblet cells. However, GTT did not affect the increase of number of goblet cells; GJT and GTT significantly increased mucin secretion from cultured HTSE cells, without significant cytotoxicity. GHJ increased mucin secretion and showed mild cytotoxicity at the highest concentration: GJT, GHJ and GTT chiefly affected the 'mucin' secretion; GJT, GHJ and GTT did not affect Ach-induced contraction of isolated tracheal smooth muscle; GTT did not significantly affect the expression levels of MUC5AC gene. However, GJT significantly. inhibit the expression levels of MUC5AC gene and GHJ significantly increased the expression levels of MUC5AC gene. These results suggest that GJT, GHJ and GTI can increase mucin secretion during short-term treatment(in vitro), whereas it can inihibit hypersecretion of mucin during long-term treatment(in vivo) and GJT and GHJ can not only affect the secretion of mucin but also affect the expression of mucin gene. Conclusions : The author suggests that the effects GJT, GHJ and GTT with their components should be further investigated and it is valuable to find, from oriental medical prescriptions, novel agents which might regulate hypersecretion of mucin from airway epithelial cells.

Primary Mucinous Cystadenocarcinoma of the Breast: Cytologic Finding and Expression of MUC5 Are Different from Mucinous Carcinoma

김성은,정우희,박지혜,홍순원,구자승,정준 대한병리학회 2012 Journal of Pathology and Translational Medicine Vol.46 No.6

Mucinous cystadenocarcinoma (MCA) in the breast is a rare neoplasm. There have been 13 cases of primary breast MCA reported. The MCA presents as a large, partially cystic mass in postmenopausal woman with a good prognosis. The microscopic findings resemble those of ovarian, pancreatic, or appendiceal MCA. The aspiration findings showed mucin-containing cell clusters in the background of mucin and necrotic material. The cell clusters had intracytoplasmic mucin displacing atypical nuclei to the periphery. Histologically, the tumor revealed an abundant mucin pool with small floating clusters of mucin-containing tumor cells. There were also small cysts lined by a single layer of tall columnar mucinous cells, resembling those of the uterine endocervix. The cancer cells were positive for mucin (MUC) 5 and negative for MUC2 and MUC6. This mucin profile is different from ordinary mucinous carcinoma and may be a unique characteristic of breast MCA.

Viscosity and wettability of animal mucin solutions and human saliva

Park, M-S,Chung, J-W,Kim, Y-K,Chung, S-C,Kho, H-S Stockton Press 2007 Oral diseases Vol. No.

<P>Objective: </P><P>The purpose of this study was to compare viscosity and wettability between animal mucin solutions and human saliva.</P><P>Materials and Methods: </P><P>Human whole and glandular saliva, porcine gastric mucin, bovine submaxillary mucin, and a mucin-based saliva substitute were used. Viscosity was measured with a cone-and-plate digital viscometer, while wettability on acrylic resin and Co–Cr alloy was determined by the contact angle.</P><P>Results: </P><P>The viscosity of animal mucin solutions was proportional to mucin concentration, with the animal mucin solution of concentration 5.0 mg ml<SUP>−1</SUP> displaying similar viscosity to stimulated whole saliva. A decrease in contact angle was found with increasing animal mucin concentration. For the saliva samples tested, viscosity increased in the following order: stimulated parotid saliva, stimulated whole saliva, unstimulated whole saliva, stimulated submandibular–sublingual saliva. Contact angles of human saliva on the tested solid phases were inversely correlated with viscosity. Contact angles of human saliva on acrylic resin were much lower than those of animal mucin solutions and of those on Co–Cr alloy (<I>P</I> < 0.01).</P><P>Conclusions: </P><P>The effectiveness of animal mucin solutions in terms of their rheological properties was objectively confirmed, indicating a vital role for mucin in proper oral function as well as the development of effective salivary substitutes.</P>

Periadnexal Mucin as an Additional Histopathologic Feature of Chronic Eczematous Dermatitis

( Noo Ri Lee ),( Jae Hong Kim ),( Hwa Young Park ),( Na Young Yoon ),( Sung Ku Ahn ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.3

Background: Cutaneous mucinoses are a heterogeneous group of disorders characterized by an abnormal amount of mucin in the skin. However, the pathomechanism of an excessive mucin deposition in the skin is still unknown. Eczematous dermatitis is sub-classified histologically into acute, subacute, and chronic variants. The characteristic histopathologic findings for chronic eczema are variable. However, periadnexal mucin deposition is not known as a feature of chronic eczema. Objective: To evaluate the presence of periadnexal mucin deposition in chronic eczematous dermatitis. Methods: We analyzed the skin biopsy specimens from 36 patients who were pathologically diagnosed with chronic eczematous dermatitis. Alcian blue, colloidal iron, and periodic acid-Schiff stains were used to evaluate the mucin deposition in histologic sections. Two dermatologists and two dermatopathologists evaluated the degree of mucin deposition using a 4-point scale. Results: Various amounts of mucin deposition were observed in the periadnexal area of patients who were diagnosed with chronic eczema. Mucin deposition was more visible after staining with mucin-specific stains. Evaluation of the staining analysis scores revealed that the staining intensities were significantly higher in patients with chronic eczema than age- and site-matched controls (normal, acute to subacute eczema, and psoriasis vulgaris). Conclusion: Periadnexal mucin (secondary mucinoses) may be an additional finding of chronic eczematous dermatitis. (Ann Dermatol 27(2) 133∼141, 2015)