http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Toshiro Ohashi,Yoshiaki Sugaya,Naoya Sakamoto,Masaaki Sato 대한의용생체공학회 2016 Biomedical Engineering Letters (BMEL) Vol.6 No.1
Purpose Vascular endothelial cells (ECs) are continuouslysubjected to mechanical forces such as fluid shear stress,stretching and hydrostatic pressure. The effect of hydrostaticpressure on EC responses has not been fully understoodcompared to that of the other two stimuli. The purpose ofthis study is to assess mechanical responses of ECs to thesemechanical stimuli. Methods Bovine aortic ECs were exposed to hydrostaticpressure of 50, 100, and 150 mmHg and fluid shear stressof 3 Pa in simultaneous or successive fashion. Immunofluorescencestaining of actin filaments and VEcadherin wasthen performed to observe cell morphology and cell-celljunctions, respectively. Results The results showed that ECs subjected to 50, 100,and 150 mmHg for 24 h elongated without predominantorientation and exhibited multilayered structure, whereassimultaneous application of 50 and 100 mmHg and 3 Pa for24 h induced marked elongation and orientation of ECsparallel to the direction of flow maintaining monolayerintegrity. This monolayer integrity was lost in ECs subjectedto 150 mmHg together with 3 Pa. A successive applicationof 100 mmHg for 24 h followed by 100 mmHg and 3 Pa for24 h, indicated that the loss of monolayer integrity due tohydrostatic pressure could not be retrieved by the followingsimultaneous application. Conclusions It can be concluded that physiological shearstress of 3 Pa is dominant to physiological hydrostatic pressureup to 100 mmHg, importantly suggesting the relativecontribution of physiological hydrostatic pressure and fluidshear stress to endothelial monolayer integrity.
Traction force microscopy for understanding cellular mechanotransduction
( Sung Sik Hur ),( Ji Hoon Jeong ),( Myung Jin Ban ),( Jae Hong Park ),( Jeong Kyo Yoon ),( Yongsung Hwang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2020 BMB Reports Vol.53 No.2
Under physiological and pathological conditions, mechanical forces generated from cells themselves or transmitted from extracellular matrix (ECM) through focal adhesions (FAs) and adherens junctions (AJs) are known to play a significant role in regulating various cell behaviors. Substantial progresses have been made in the field of mechanobiology towards novel methods to understand how cells are able to sense and adapt to these mechanical forces over the years. To address these issues, this review will discuss recent advancements of traction force microscopy (TFM), intracellular force microscopy (IFM), and monolayer stress microscopy (MSM) to measure multiple aspects of cellular forces exerted by cells at cell-ECM and cell-cell junctional intracellular interfaces. We will also highlight how these methods can elucidate the roles of mechanical forces at interfaces of cell-cell/cell-ECM in regulating various cellular functions. [BMB Reports 2020; 53(2): 74-81]