F344 rats를 이용 망간 및 철 화합물의 체내 흡수와 생식기 및 운동성에 미치는 영향

김현영 ( Hyeon Yeong Kim ),이성배 ( Sung Bae Lee ),한정희 ( Jeong Hee Han ),최성봉 ( Sung Bong Choi ),강민구 ( Min Gu Kang ),이채관 ( Chae Kwan Lee ) 한국산업위생학회 2007 한국산업보건학회지 Vol.17 No.3

This study aimed to examine the harmful effects of Mn and Fe, which may be generated as dust or fume in the industrial sites, on the body and genital organs by their inhalation. It is intended to find the characteristics and differences of the hazardousness by inhaling a single and the mixed materials of Mn and Fe. Male F344 rats were divided into the control group and 3 exposed groups on the basis of the test material compound (Mn 1.5 mg/㎥ Mn 1.5 and Fe 3.0 mg/㎥, Fe 3.0 mg/㎥). The 4 groups were divided into 4 subgroups again on the basis of the exposure period (4 and 13 weeks) and the recovery period (4 and 13 weeks). The exposure condition was 6 hours a day, 5 days a week for the whole body. Clinical tests including changes in weight and feed rate, blood biochemical test, motility change, changes in the number and the amount of spermatozoon (sperm count), daily sperm production (DSP), deformity test of spermatozoon and changes in the accumulation of Mn and Fe in blood and internal organs were performed. Motility was reduced by Mn exposure. Especially, the effect of Mn was exposure period responsible. By mixing with Fe, no significant change in motility Mn and Fe accumulation in organs was observed. Sperm count and daily sperm production (DSP) were decreased by Mn. Additional effect like the reduction of sperm count and DSP, and delayed restoration of sperm count and DSP during the recovery period were observed in the mixed exposure group. These results indicate that Mn and Fe may affect the motility reduced and has male reproductive toxicity. Mixed exposure of Mn and Fe lead to synergic effects on the male reproductive toxicity.

연소 시험에서 발생하는 일산화탄소와 이산화탄소의 발생

정영진 한국화재소방학회 2015 한국화재소방학회논문지 Vol.29 No.5

This study was performed to test the production of combustion toxic gases by Pinus rigida specimens treated with varioustypes of methylpiperazinomethyl-bis-phosphonic acid Mn+ (PIPEABPMn+) and methylpiperazinomethyl-bis-phosphonicacid (PIPEABP). Three coats of 15 wt% PIPEABPMn+ and PIPEABP solutions were applied to plates of Pinusrigida at room temperature. After drying the treated specimens, the production of combustion toxic gases was examinedusing a cone calorimeter (ISO 5660-1). The specimens treated with PIPEABPMn+ showed lower carbon monoxide production(COpeak; 0.0136~0.0178% at 532~678 s) than the PIPEABP plates, except for the specimen treated with PIPEABPFe3+. In addition, the peak carbon dioxide production (CO2 peak) was lower (0.03648~0.3648% at 373~433 s) than that ofthe PIPEABP-treated plate. Notably, oxygen production was much higher than 15%, which can be fatal to humans. Therefore,the resulting risk could be eliminated. The results indicate that the combustion toxicities were partially decreased dueto treatment of the virgin plate with PIPEABPMn+. 본 연구에서는 메틸렌피페라지노메틸-비스-포스폰산 금속염(PIPEABPMn+)과 메틸렌피페라지노메틸-비스-포스폰산(PIPEABP)으로 처리된 리기다 소나무의 연소독성가스의 생성을 시험하였다. 15 wt%의 메틸렌피페라지노메틸-비스-포스폰산 금속염과 메틸렌피페라지노메틸-비스-포스폰산 수용액으로 각각 리기다 소나무에 3회 붓칠하여 실온에서 건조시킨후, 콘칼로리미터(ISO 5660-1)를 이용하여 연소독성가스의 생성을 시험하였다. 그 결과, 메틸렌피페라지노메틸-비스-포스폰산 금속염으로 처리한 시험편은 메틸렌피페라지노메틸-비스-포스폰산 철염(PIPEABPFe2+)으로 처리한 시험편을 제외하고, 메틸렌피페라지노메틸-비스-포스폰산을 처리한 시험편과 비교하여 최대일산화탄소의 생성(CO peak production)이(0.0136~0.0178% at 532~678 s)으로 낮게 나타났다. 그리고 금속염으로 처리한 시험편(PIPEABPMn+)은 금속염으로 처리하지 않은 시험편(PIPEABP)보다 낮은 최대이산화탄소의 생성(CO2 peak production)이 (0.0537~0.0628% at 532~678 s)임을 보였다. O2의 생성농도는 사람에게 치명적일 수 있는 수준인 15%보다는 훨씬 높으므로 그로 인한 위험성은배제할 수 있었다. 따라서 메틸렌피페라지노메틸-비스-포스폰산 금속염으로 처리한 시험편은 처리 하지 않은 시험편과비교하여 연소-유독성을 부분적으로 감소시켰다.

Syntheses and Characterizations of Serine and Threonine Capped Water-Dispersible ZnS:Mn Nanocrystals and Comparison Study of Toxicity Effects on the growth of E. coli by the Methionine, Serine, Threonine, and Valine Capped ZnS:Mn Nanocrystals

Lim, Eun-Ju,Park, Sang-Hyun,Byun, Jong-Hoe,Hwang, Cheong-Soo Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.5

Water-dispersible ZnS:Mn nanocrystals were synthesized by capping the surface of the nanocrystals with conventional aminoacids ligands: serine and threonine. The aminoacids capped ZnS:Mn nanocrystal powders were characterized by XRD, HR-TEM, EDXS, ICP-AES and FT-IR spectroscopy. The optical properties were also measured by UV/Vis and solution photoluminescence (PL) spectroscopies in aqueous solvents. The solution PL spectra showed broad emission peaks around 600 nm with PL efficiencies of 9.7% (ZnS:Mn-Ser) and 15.4% (ZnS:Mn-Thr) respectively. The measured particle sizes for the aminoacid capped ZnS:Mn nanocrystals by HR-TEM images were about 3.0-4.0 nm, which were also supported by Debye-Scherrer calculations. In addition, cytotoxic effects of four aminoacids capped ZnS:Mn nanocrsystals over the growth of wild type E. coli were investigated. Although toxicity in the form of growth inhibition was observed with all the aminoacids capped ZnS:Mn nanocrystals at higher dose (1 mg/mL), ZnS:Mn-Met and ZnS:Mn-Thr appeared non-toxic at doses less than 100 ${\mu}g$/mL. Low biological toxicities were seen at doses less than 10 ${\mu}g$/ mL for all nanocrystals.

Syntheses and Characterizations of Serine and Threonine Capped Water-Dispersible ZnS:Mn Nanocrystals and Comparison Study of Toxicity Effects on the growth of E. coli by the Methionine, Serine, Threonine, and Valine Capped ZnS:Mn Nanocrystals

임은주,박상현,변종회,황청수 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.5

Water-dispersible ZnS:Mn nanocrystals were synthesized by capping the surface of the nanocrystals with conventional aminoacids ligands: serine and threonine. The aminoacids capped ZnS:Mn nanocrystal powders were characterized by XRD, HR-TEM, EDXS, ICP-AES and FT-IR spectroscopy. The optical properties were also measured by UV/Vis and solution photoluminescence (PL) spectroscopies in aqueous solvents. The solution PL spectra showed broad emission peaks around 600 nm with PL efficiencies of 9.7% (ZnS:Mn-Ser) and 15.4% (ZnS:Mn-Thr) respectively. The measured particle sizes for the aminoacid capped ZnS:Mn nanocrystals by HR-TEM images were about 3.0-4.0 nm, which were also supported by Debye-Scherrer calculations. In addition, cytotoxic effects of four aminoacids capped ZnS:Mn nanocrsystals over the growth of wild type E. coli were investigated. Although toxicity in the form of growth inhibition was observed with all the aminoacids capped ZnS:Mn nanocrystals at higher dose (1 mg/mL), ZnS:Mn-Met and ZnS:Mn-Thr appeared non-toxic at doses less than 100 μg/mL. Low biological toxicities were seen at doses less than 10 μg/ mL for all nanocrystals.

Syntheses of Biologically Non-Toxic ZnS:Mn Nanocrystals by Surface Capping with O-(2-aminoethyl)polyethylene Glycol and O-(2-carboxyethyl)polyethylene Glycol Molecules

Kong, Hoon-Young,Song, Byung-Kwan,Byun, Jonghoe,Hwang, Cheong-Soo Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.4

Water-dispersible ZnS:Mn nanocrystals were synthesized by capping the surface of the nanocrystal with O-(2-Aminoethyl)polyethylene glycol (PEG-$NH_2$, Mw = 10,000 g/mol) and O-(2-Carboxyethyl)polyethylene glycol (PEG-COOH, Mw = 10,000 g/mol) molecules. The modified PEG capped ZnS:Mn nanocrystal powders were thoroughly characterized by XRD, HR-TEM, EDXS, ICP-AES and FT-IR spectroscopy. The optical properties were also measured by UV/Vis and photoluminescence (PL) spectroscopies. The PL spectra showed broad emission peaks at 600 nm with similar PL efficiencies of 7.68% (ZnS:Mn-PEG-NH2) and 9.18% (ZnS:Mn-PEG-COOH) respectively. The measured average particle sizes for the modified PEG capped ZnS:Mn nanocrystals by HR-TEM images were 5.6 nm (ZnS:Mn-PEG-NH2) and 6.4 nm (ZnS:Mn-PEG-COOH), which were also supported by Debye-Scherrer calculations. In addition, biological toxicity effects of the nanocrystals over the growth of wild type E. coli were investigated. They showed no biological toxicity to E. coli until very high concentration dosage of 1 mg/mL of the both nanocrystal samples.

Syntheses of Biologically Non-Toxic ZnS:Mn Nanocrystals by Surface Capping with O-(2-aminoethyl)polyethylene Glycol and O-(2-carboxyethyl)polyethylene Glycol Molecules

공훈영,Byungkwan Song,변종회,황청수 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.4

Water-dispersible ZnS:Mn nanocrystals were synthesized by capping the surface of the nanocrystal with O-(2- Aminoethyl)polyethylene glycol (PEG-NH2, Mw = 10,000 g/mol) and O-(2-Carboxyethyl)polyethylene glycol (PEG-COOH, Mw = 10,000 g/mol) molecules. The modified PEG capped ZnS:Mn nanocrystal powders were thoroughly characterized by XRD, HR-TEM, EDXS, ICP-AES and FT-IR spectroscopy. The optical properties were also measured by UV/Vis and photoluminescence (PL) spectroscopies. The PL spectra showed broad emission peaks at 600 nm with similar PL efficiencies of 7.68% (ZnS:Mn-PEG-NH2) and 9.18% (ZnS:Mn- PEG-COOH) respectively. The measured average particle sizes for the modified PEG capped ZnS:Mn nanocrystals by HR-TEM images were 5.6 nm (ZnS:Mn-PEG-NH2) and 6.4 nm (ZnS:Mn-PEG-COOH), which were also supported by Debye-Scherrer calculations. In addition, biological toxicity effects of the nanocrystals over the growth of wild type E. coli were investigated. They showed no biological toxicity to E. coli until very high concentration dosage of 1 mg/mL of the both nanocrystal samples.

Biological Toxicity Changes of Mercaptoacetic Acid and Mercaptopropionic Acid Upon Coordination onto ZnS:Mn Nanocrystal

Kong, Hoon-Young,Hwang, Cheong-Soo,Byun, Jong-Hoe Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

Mercaptoacetic acid (MAA) and mercaptopropionic acid (MPA) capped ZnS:Mn nanocrystals were synthesized and their physical characteristics were examined by XRD, HR-TEM, EDXS, and FT-IR spectroscopy. The optical properties of the MPA capped ZnS:Mn nanocrystals dispersed in aqueous solution were also measured by UV/Vis and solution photoluminescence (PL) spectra, which showed a broad emission peak around 598 nm (orange light emissions) with calculated relative PL efficiency of 5.2%. Comparative toxicity evaluation of the uncoordinated ligands, MAA and MPA, with the corresponding ZnS:Mn nanocrystals revealed that the original ligands significantly suppressed the growth of wild type E. coli whereas the ligandcapped nanocrystals did not show significant toxic effects. The reduced cytotoxicity of the conjugated ZnS:Mn nanocrystals was also observed in NIH/3T3 mouse embryonic fibroblasts. These results imply that potential toxicities of the capping ligands can be neutralized on ZnS:Mn surface.

Biological Toxicity Changes of Mercaptoacetic Acid and Mercaptopropionic Acid Upon Coordination onto ZnS:Mn Nanocrystal

공훈영,황청수,변종회 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

Mercaptoacetic acid (MAA) and mercaptopropionic acid (MPA) capped ZnS:Mn nanocrystals were synthesized and their physical characteristics were examined by XRD, HR-TEM, EDXS, and FT-IR spectroscopy. The optical properties of the MPA capped ZnS:Mn nanocrystals dispersed in aqueous solution were also measured by UV/Vis and solution photoluminescence (PL) spectra, which showed a broad emission peak around 598 nm (orange light emissions) with calculated relative PL efficiency of 5.2%. Comparative toxicity evaluation of the uncoordinated ligands, MAA and MPA, with the corresponding ZnS:Mn nanocrystals revealed that the original ligands significantly suppressed the growth of wild type E. coli whereas the ligandcapped nanocrystals did not show significant toxic effects. The reduced cytotoxicity of the conjugated ZnS:Mn nanocrystals was also observed in NIH/3T3 mouse embryonic fibroblasts. These results imply that potential toxicities of the capping ligands can be neutralized on ZnS:Mn surface.

석회류제의 시용이 엽연형 황피발생 억제에 미치는 영향

이태수,김명수,홍순근,Lee, Tae-Su,Kim, Myeong-Su,Hong, Sun-Geun 고려인삼학회 1990 Journal of Ginseng Research Vol.14 No.1

Effect of liming material application on the prevention or cure of Mn toxicity symptoms including marginal leaf chlorosis in 3-year-old ginseng plants grown in acidic soils were investigated. It was found that the ratio of Fe to Mn was above about 0.5 and the ratio of Mn to Fe was below about 2.00 in 4-year old ginseng leaves when liming materials were applied in field experiments. It was apparent that the occurrence of marginal leaf chlorosis was decreasing affected by application of Ca, Mg and Fe. The appearance of marginal leaf chlorosis was decreased to about 78 percent in 4-year-old ginseng and to about 69 percent in 5-year-old ginseng, and then the fresh root weight was increased up to 66 percent in 4-year ginseng plants.