http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이규민,Zoe K. Scott-Nevros,이상목,김동혁 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.6
Methylobacterium is a genus belonging to the pink-pigmented facultative methylotrophs, which can use C1 substrates as a sole carbon source. Methylorubrum extorquens (formerly Methylobacterium extorquens) is a potentially valuable bacteria in methanol-based bioindustry producing high-value-added chemicals. Thus, it is critical to understand the core metabolic pathways of M. extorquens strains for further advancement as a platform bacterium in the bioindustry. However, there are no reported systems biological approaches comparing different M. extorquens strains. This paper focuses on comparing the genomic properties of seven completely sequenced M. extorquens strains, including M. extorquens PA1, which was re-sequenced, and M. extorquens ATCC 55366, which was newly sequenced in this study. Pan-genome analysis indicated that a total of 10,431 ortholog clusters composed the pan-genome of these M. extorquens strains, including 3,507 core genome, 1,860 accessory genome, and 5,064 unique genome clusters. The functional annotation discovered that genes related to signal transduction were the most abundant in the pan-genome. Pan-genome analysis of two different habitat-specific groups revealed that the strains isolated from various soil environments had a higher percentage of genes involved in mobilome in their core genome. Subsequent exploration for secondary metabolite biosynthesis clusters denoted the unique ability of M. extorquens AM1 to produce various toblerols. M. extorquens PA1 could be a strong candidate for industrial strain development because of its minimal genome size with fully conserved central metabolism. This study provides the first inclusive insight into the differences in genomic characteristics of central metabolic networks of M. extorquens.
윤지희,오민규 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
M. extorquens uses C1 compounds as a sole carbon source, so it has been in spotlight. Formate could be synthesized from CO<sub>2</sub>, regarded as environmental, sustainable C1 feedstock. In this study, we produced lycopene from formate using M. extorquens by engineering nonmevalonoate pathway, also called MEP pathway, for effective lycopene production. First, LMZ01 strain was constructed by deleting CrtCD, genes for consuming lycopene, and used as a parental strain, showing improved lycopene production. Then, dxs, a rate-limiting step of MEP pathway, was overexpressed under lac, tac, and mxaF promoter, respectively. Expression level was shown to be strong in the order of mxaF, tac, and lac promoter by expressing EGFP. Interestingly, while the mxaF promoter with strong expression level was ineffective, the tac promoter with moderate level showed 45% increase in lycopene production. This showed lycopene could be produced reasonably from formate and moderate expression of MEP pathway is crucial.
Crystal Structure of Mesaconyl-CoA Hydratase from Methylorubrum extorquens CM4
Ahn Jae-Woo,Hong Jiyeon,Kim Kyung-Jin 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.4
Methylorubrum extorquens, a facultative methylotroph, assimilates C1 compounds and accumulates poly-β-hydroxylbutyrate (PHB) as carbon and energy sources. The ethylmalonyl pathway is central to the carbon metabolism of M. extorquens, and is linked with a serine cycle and a PHB biosynthesis pathway. Understanding the ethylmalonyl pathway is vital in utilizing methylotrophs to produce value-added chemicals. In this study, we determined the crystal structure of the mesaconyl-CoA hydratase from M. extorquens (MeMeaC) that catalyzes the reversible conversion of mesaconyl-CoA to β-methylmalyl-CoA. The crystal structure of MeMeaC revealed that the enzyme belongs to the MaoC-like dehydratase domain superfamily and functions as a trimer. In our current MeMeaC structure, malic acid occupied the substrate binding site, which reveals how MeMeaC recognizes the β-methylmalyl-moiety of its substrate. The active site of the enzyme was further speculated by comparing its structure with those of other MaoC-like hydratases.
대사공학이 적용된 Methylorubrum extorquens AM1에서 개미산을 유일 탄소원으로 라이코펜 생산
김우영,윤지희,김승진,양윤용,최성호,허문석,이병희,오민규 한국생물공학회 2023 KSBB Journal Vol.38 No.1
Formate is a C1 compound that can be converted from carbon dioxide, the main chemical causing global warming. In this study, we produced lycopene from formate using Methylorubrm extorquens AM1 by engineering nonmevalonoate pathway. Through metabolic engineering, lycopene production of the strain increased by 229.3% in the medium using methanol as a carbon source. Then, to utilize formate as a sole carbon source effectively, formate assimilation protein, FtfL was overexpressed. As a result of formatebasaed fed-batch fermentation, the lycopene production increased by 157%. Lycopene production using formate as a sole carbon source has not been reported yet and this study showed that engineered M. extorquens AM1 can be a candidate strain that produces carotenoids from formate.
장우진,윤지희,오민규 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.2
Deriving both carbon and energy from formate, a single-carbon substrate, for the microbial production of value-added products allows its use as the main feedstock in biorefinery, with consequent environmental and economic benefits. Methylorubrum extorquens AM1, a strain capable of growth solely on formate, is a known producer of the biopolymer polyhydroxyalkanoate (PHA), during nitrogendeficient growth. Based on findings from our previous report, the gene ftfL was selected for overexpression to enhance growth and PHA production using formate. Its overexpression in a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)-producing strain yielded improved growth and a 1.4-fold increase in PHBV production. The strain could thus be tested for long-term fermentation, intended for optimized growth and production in formate. A customized fermentation regimen was established by incorporating both the conventional two-phase fermentation method for PHA production and a repeated fed-batch fermentation process designed to resolve the problem of sodium accumulation. The poly-3-hydroxybutyrate and PHBV copolymer-producing strains resulted in 11.07 g/L and 2.76 g/L of bio-degradable polymers, respectively, in the fermentation process.