대기중 휘발성 유기화합물질의 검출한계와 정량한계에 대한 연구

이상열,안규동 순천향대학교 산업의학연구소 1997 순천향산업의학 Vol.3 No.1

Volatile organic compounds(VOCs) are emitted as air pollutant from many petrochemical process. The measurement for these materials are difficult, because of a very little amount existed in atmosphere. This study was conducted in order to investigate the limit of detection(LOD) and limit of quantitaion(LOQ) for VOCs measurement. The concentrations of VOC's LOD and LOQ were assayed by gas chromatography with flame ionization detection(GC/FID). The results are as follows; 1. LOD and LOQ of acrylonitrile were 0.0114㎍/㎖, 0.0144㎍/㎖, respectively. 2. LOD and LOQ of benzene were 0.0136㎍/㎖, 0.0150㎍/㎖, respectively. 3. LOD and LOQ of 1.3-butadiene were 0.0114㎍/㎖, 0.0136㎍/㎖, respectively. 4. LOD and LOQ of 2-butanone was 0.1016㎍/㎖, 0.1073㎍/㎖, respectively. 5. LOD and LOQ of carbon Tetrachloride were 0.0065㎍/㎖, 0.0188㎍/㎖, respectively. 6. LOD and LOQ of c--hexane were 0.0112㎍/㎖, 0.0143㎍/㎖, respectively. 7. LOD and LOQ of ethylbenzene were 0.0112㎍/㎖, 0.0131㎍/㎖, respectively. 8. LOD and LOQ of n-hexane were 0.0172㎍/㎖, 0.0582㎍/㎖, respectively. 9. LOD and LOQ of styrene were 0.0122㎍/㎖, 0.0142㎍/㎖, respectively. 10. LOD and LOQ of tetrachloroethylene were 0.0121㎍/㎖, 0.0320㎍/㎖, respectively. 11. LOD and LOQ of 1.1.1-trichloroethane were 0.0016㎍/㎖, 0.0062㎍/㎖, respectively. 12. LOD and LOQ of trichloroethylene were 0.0064㎍/㎖, 0.0183㎍/㎖, respectively. 13. LOD and LOQ of toluene were 0.0136㎍/㎖, 0.0148㎍/㎖, respectively. 14. LOD and LOQ of xylene were 0.0140㎍/㎖, 0.0154㎍/㎖, respectively.

Urinary bisphenol A versus serum bisphenol A concentration and ovarian reproductive outcomes among IVF patients: Which is a better biomarker of BPA exposure?

Seung Hee Cho,Yohan Choi,Sean Hyungwoo Kim,김상진,Jongwha Chang 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.4

Bisphenol A (BPA) is an endocrine-disrupting compound (EDC) that is used widely in commercial products in the production of polycarbonate plastics for baby and water bottles, epoxy resins for lacquer lining of food and beverage cans and water pipes, dental sealants, dental composites and thermal receipts paper. There is inhibitory effect of BPA on nuclear estrogen (E2) production in granulosa cells of developing follicles that disrupt normal development to the antral follicles via suppression of E2 in granulosa cells of developing follicles during the menstrual cycle followed by reduction in the number of oocytes retrieved in in-vitro fertilization (IVF) patients. Several studies corroborate an inverse association between serum and/or urinary BPA concentration and the IVF outcome: Peak E2 levels and the number of oocytes retrieved. Upon oral ingestion, 99.5% of unconjugated parent BPA (free BPA) is metabolized to either BPA glucuronide (BPA-G) or BPA sulfate (BPA-S). The unconjugated BPA can bind to the estrogen receptors(ER) while conjugated BPA (biologically inactive BPA) do not bind the estrogen receptor(ER). The challenge is to assess the relationship between BPA exposure among infertile patients with respect to follicular response and health during IVF. The establishment of temporal sequence between BPA exposure and infertility would be the research question to answer: Which route is a better biomarker? The advantages of urine BPA collection would provide pragmatic advantages for clinicians in order to practice cost-effective medicine. However, unconjugated BPA measurement (compared to total BPA) introduces challenges in measurement accuracy since unconjugated BPA requires higher magnitude of limit of detection (LOD) with higher risk of contamination from the medical equipment. The difference in route of BPA assessment could introduce bias in the interpretation of results in terms of the association between BPA levels and the number of oocytes. Fujimoto et al. and Bloom et al. analyzed the relationship between serum BPA and IVF outcome in infertile women. It may sound hypothetically justified due to utilizing serum unconjugated BPA, this strategy is not successful in choosing a practical biomarker of BPA exposure due to toxicokinetic properties of BPA metabolism and excretion in humans.

A review of techniques for the determination of polycyclic aromatic hydrocarbons in air

Pandey, S.K.,Kim, K.H.,Brown, R.J.C. Elsevier Scientific Pub. Co 2011 Trends in analytical chemistry Vol.30 No.11

We provide an extensive review of the common methodologies employed in the analysis of airborne polycyclic aromatic hydrocarbons (PAHs). The review focuses on gas-chromatography-based approaches, in the light of their universal application with excellent separation, resolution, and sensitivity. We first describe collection methods for airborne PAHs in the gas and particle phases. We then evaluate the efficiency of extraction techniques employed for separating target PAHs from sampling media, using conventional solvent-based and emerging thermal-desorption approaches. We also describe commonly employed analytical methods with respect to their applicability to PAHs in gas and particle phases, collected from diverse environmental settings. As an essential part of basic quality assurance, we examine each method with special emphasis on key parameters (e.g., limit of detection and reproducibility). Finally, we address the likely directions of methodological developments, their limitations, and the future prospects for PAH analysis.

Carbon Nanotube Film을 이용한 액체 전기화학 센서

노재하 ( Jaeha Noh ),안상수 ( Sangsu An ),이창한 ( Changhan Lee ),이상태 ( Sangtae Lee ),이문진 ( Moonjin Lee ),서동민 ( Dongmin Seo ),장지호 ( Jiho Chang ) 한국센서학회 2021 센서학회지 Vol.30 No.4

We studied electrochemical sensors using printed carbon nanotube (CNT) film on a polyethylene terephthalate (PET) substrate. Multiwalled CNT films were printed on a PET substrate to study its feasibility as hazardous and noxious substances (HNS) detection sensor. The printed CNT film (PCF) with a 50 μm thickness exhibited a specific resistance of 230 ohm. To determine the optimum sensor structure, a resistance-type PCF sensor (R-type PCF sensor) and a conductive-type PCF sensor (C-type PCF sensor) were fabricated and compared using diluted NH3 droplets with various concentrations. The response magnitude, response time, sensitivity, linearity, and limit of detection (LOD) were compared, and it was concluded that the C-type PCF sensor exhibited superior performance. By applying a C-Type PCF sensor, we confirmed the detection performance of 12 types of floating HNS and the response of the sensor with selectivity according to the degree of polarity.

교반막대 추출법과 GC-MS/MS를 이용한 수중의 합성 향물질류 분석

서창동(Chang Dong Seo),손희종(Hee Jong Son),염훈식(Hoon Sik Yoom),최진택(Jin Taek Choi),류동춘(Dong Choon Ryu),권기원(Ki Won Kwon),장성호(Seung Ho Jang) 大韓環境工學會 2014 대한환경공학회지 Vol.36 No.6

본 연구에서는 SBSE 전처리 장치와 GC-MS/MS를 이용하여 합성 향물질 11종을 동시 분석할 수 있는 분석법을 개발하기 위해 흡착 bar의 교반시간, 교반속도, 시료수의 pH, 시료수 용량, 염석제 투입량 및 메탄올 주입량 변화 등 SBSE (stir barsorptive extraction) 전처리 조건과 GC-MS/MS (gas chromatography/tandem mass spectrometry)의 기기조건을 다양하게 변화시켜 SBSE-GC-MS/MS를 이용한 분석법을 개발하였다. 11종의 합성 향물질들에 대한 검출한계(LOD)는 2.1~4.1 ng/L였으며, 정량한계(LOQ)는 6.6~12.9 ng/L였다. 수돗물, 낙동강 원수, 하수처리장 최종방류수 및 해수를 이용하여 시료수의 matrix 영향을살펴본 결과, 11종의 합성 향물질들의 회수율 및 RSD의 경우 각각 88%~119% 및 0.8%~7.5%로 양호한 결과를 나타내어 시료수의 matrix 영향을 받지 않는 것으로 나타났다. 본 연구에서 개발된 SBSE-GC-MS/MS 분석법은 40 mL 정도의 적은 시료수량으로도 고감도 분석이 가능하며, 용매류를 사용하지 않기 때문에 분석자의 건강 및 환경친화적인 분석법이라는 장점뿐만 아니라 간편하고, 빠르며 자동화된 방법이라는 장점을 가진다. A highly sensitive analytical method based on stir bar sorptive extraction (SBSE) technique and gas chromatography/tandem mass spectrometry (GC-MS/MS) has been developed, allowing the simultaneous multi-analyte determination of 11 syntheticfragrances (SFs) in water samples. The stir bar coated with polydimethylsiloxane (PDMS) was added to 40 mL of water sample atpH 3 and stirred at 1,100 rpm for 120 min. Other SBSE parameters (salt effect and presence of organic solvent) were optimised. The method shows good linearity (coefficients > 0.990) and reproducibility (RSD < 10.9%). The extraction efficiencies were above83% for all the compounds. The limits of detections (LOD) and limits of quantification (LOQ) were 2.1~4.1 ng/L and 6.6~12.9ng/L, respectively. The developed method offers the ability to detect 11 SFs at ultra-low concentration levels with only 40 mL ofsample volume. Matrix effects in tap water, river water, wastewater treatment plant (WWTP) final effluent water and seawaterwere investigated and it was shown that the method is suitable for the analysis of trace level of 11 SFs. The method developedin the present study has the advantage of being rapid, simple, high-sensitive and both user and environmentally friendly.

Biochemistry,Molecular Biology ; Qualitative PCR Method for Detection of Genetically Modified Maize Lines NK603 and TC1507

Seong Hun Lee,Yong Hwan Park,Jin Kug Kim,Keun Woo Park,Young Mi Kim 한국응용생명화학회 2004 Journal of Applied Biological Chemistry (J. Appl. Vol.47 No.4

Lateral flow immunoassay에서의 나노입자의 활용

이창우,목영준,( Ramu Errabelli ),최승훈,주후돈,최의열 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1

면역크로마토그래피 방법은 항원에 대한 항체의 특이적인 면역적 반응성과 발색특성을 나타내는 입자 및 유동성 막의 모세관현상에 의한 분자의 이동을 응용한 진단검사 방법이다. 샘플 내 미량으로 존재하는 마커의 탐지가 가능하다면 조기진단을 통한 환자의 치료성과를 향상시킬 수 있기 때문에 많은 연구자들이 분석기기의 검출한계를 극대화시키기 위해 노력을 기울이고 있으며, 여기에는 신호증폭 또는 시료분리를 목적으로 다양한 나노입자들이 개발, 적용되고 있다. 이에 본 발표에서는 전통적으로 사용되고 있는 금나노입자를 포함하여 다양한 나노입자들의 역할과 활용 가능성을 대표적인 면역크로마토그래피 방법인 측방유동분석법 (lateral flow assay)을 통해 논의해 보고자 한다.

Sensitivity Enhancement of Bead-based Electrochemical Impedance Spectroscopy (BEIS) biosensor by electric field-focusing in microwells

Shin, Kyeong.Sik.,Ji, J.H.,Hwang, K.S.,Jun, S.C.,Kang, J.Y. Elsevier Applied Science 2016 Biosensors & bioelectronics Vol.85 No.-

This paper reports a novel electrochemical impedance spectroscopy (EIS) biosensors that uses magnetic beads trapped in a microwell array to improve the sensitivity of conventional bead-based EIS (BEIS) biosensors. Unloading the previously measured beads by removing the magnetic bar enables the BEIS sensor to be used repeatedly by reloading it with new beads. Despite its recyclability, the sensitivity of conventional BEIS biosensors is so low that it has not attracted much attentions from the biosensor industry. We significantly improved the sensitivity of the BEIS system by introducing of a microwell array that contains two electrodes (a working electrode and a counter electrode) to concentrate the electric field on the surfaces of the beads. We confirmed that the performance of the BEIS sensor in a microwell array using an immunoassay of prostate specific antigen (PSA) in PBS buffer and human plasma. The experimental results showed that a low concentration of PSA (a few tens or hundreds of fg/mL) were detectable as a ratio of the changes in the impedance of the PBS buffer or in human plasma. Therefore, our BEIS sensor with a microwell array could be a promising platform for low cost, high-performance biosensors for applications that require high sensitivity and recyclability.