LDH를 함유한 PTMSP 복합막의 기체투과 특성

정연임,홍세령 한국막학회 2012 멤브레인 Vol.22 No.5

본 연구에서는 높은 투과도를 갖는 PTMSP에 1, 3, 5 wt% LDH 함량으로 용액 삽입법에 의하여 PTMSP/LDH 복합막을 제조하였다. 복합막의 물리화학적 특성은 FT-IR, TGA, XRD, UTM, SEM을 사용하여 조사하였고, H_2, N2, CO2 기체에 대한 투과도와 선택도 성질을 고찰하였다. PTMSP/LDH 복합막의 기체 투과도는 LDH 함량이 증가함에 따라 감소하였고, H2와 CO2의 선택도는 LDH의 함량이 5 wt%일 때 최대값을 보였다. 그리고 PTMSP와 PTMSP/LDH 복합막은 기체투과 압력이 증가함에 따라 기체의 투과도가 증가하였다. PTMSP/LDH 복합막의 기체투과도는 PTMSP 막에 비해 압력 증가에 대한 차이가 다소 작게 나타났다. In this study, PTMSP/LDH composite membranes were prepared by the solution intercalation method with 1, 3, and 5 wt% LDH contents to PTMSP. To investigate the physico-chemical characteristics of composite membranes, the analytical methods such as FT-IR, TGA, XRD, UTM, and SEM have been utilized, and the gas permeability and selectivity properties of H2, N2, and CO2 were evaluated. The permeability of the PTMSP/LDH composite membranes decreased as LDH content increased and the selectivity H2 and CO2 showed the maximum value at 5 wt% of LDH content. Permeability of PTMSP/LDH composite membrane increased as the gas permeation pressure increased. The difference of the increase in gas permeation pressure of the permeability of PTMSP/LDH composite membrane was slightly smaller than of PTMSP membrane.

LDH/ AChE and LDH/BChE Ratios (Paralichthys olivaceus) as Biomarkers of Coastal Pollution on Coast of Korea.

Choi Jin-Ho,Kim Dong-Woo,Kim Chang-Mok,Yang Dong Beom The Korean Society of Fisheries and Aquatic Scienc 1999 Fisheries and Aquatic Sciences Vol.2 No.2

This study was designed to develop biomarkers of coastal pollution using biochemical indices of flounder (Paralichthys olivaceus) by changes in lactate dehydrogenase (LDH) activity in the serum and cholinesterase activities in brain membranes. For this purpose acetylcholiesterase (AChE) activity, butyrylcholinesterase (BChE) activity, LDH/AChE ratio and LDH/BChE ratio of cultured flounders at 5 different sites on the southern coast of Korea were compared to those of wild flounders caught in the Pohang, eastern coast of Korea as a control group. Relatively high LDH activities were measured in the serum of flounders cultured on the southern coast of Korea (0.101-0.145 unit) than those in the Pohang control group (0.093 unit). AChE activities were significantly low $(about\;10-20\%)$ in brain membranes of cultured flounders compared to those in the Pohang control group. The ratios of LDH/AChE and LDH/BChE were consistently higher $(136-178\%,\; 155-214\%)$ in cultured flounders than those of Pohang control group. Thus, we propose that the ratios of LDH/AChE and LDH/BChE in flounders could be applicable for the diagnosis of marine pollution.

각종 질환에 있어서 혈청 Isoenzyme의 전기영동상

김인자,김재식,김중명 慶北大學校 醫科大學 1986 慶北醫大雜誌 Vol.27 No.4

최대 및 환기역치 운동 시 근테이핑이 LDH 및 Isoenzyme에 미치는 영향

정종윤(Joung Yun Jung),서해근(Hae Geun Seo) 한국사회체육학회 2009 한국사회체육학회지 Vol.0 No.37

The purpose of this study is to investigate how taping attachment influence the change of LDH and isoenzyme in blood after lower limbs taping group, whole body taping group, and control group were respectively divided with 7 members based on the male university students. The results of this study are as follows: While total-LDH, LDH1, and LDH2 respectively decreased in exercise at maximum, LDH3, LDH4, and LDH5 increased in the change of all LDHs. And then all LDHs were, in the post-hoc results, found to have a significant difference in CG and WTG among all groups, and also to have a significant difference right after the exercise as well as at the 30mins of recovery. While total-LDH, LDH1, and LDH2 respectively decreased, LDH4 and LDH5 increased in exercise at ventilation threshold. And then total-LDH, LDH1, LDH4, and LDH5 were, in the post-hoc results, found to have a significant increase in CG and WTG and also to have a significant difference right after the exercise as well as at the 30mins of recovery. As the above conclusion, taping attachment was, in exercise at maximum and ventilation threshold, found to have a positive effect on the improvement of LDH and isoenzyme in blood. Taping is recognized to be ergogenic aids to help the practice of effective exercise and the prevention of injury in sports. help the practice of effective exercise and the prevention of injury in sports.

각종 질환에 있어서 LDH Isoenzyme의 전기영동상

김인자,김재식,김중명 慶北大學校 醫科大學 1986 慶北醫大誌 Vol.27 No.4

최근 약 1년동안 경북대학교 의과대학부속병원 임상병리과에 의뢰되었던 혈청 LDH활성도와 LDH isoenzyme전기영동검사를 총 359예와 정상인 대조군 100명에 대하여 임상적의의를 검토하기 위하여 심시한 결과를 다음과 같이 요약할 수 있었다. 심근경색에 있어서는 LDH활성도는 1023.0±132.73 U/L로서 저명한 증가를 보였고 isoenzyme LDH_1이 증가의 경향을 보여 특징적이었고 LDH_5의 증가는 2차적인 간울혈로 추정하였다. 울혈성심부전에 있어서 LDH활성도는 650.3±42.38 U/L로서 중등도의 증가를 보였으며 이의 증가는 LDH_4 및 LDH_5의 증가에 의한 것이었다. 급성간질환에 있어서 LDH활성도는 급성간염에서 769.75±74.37 U/L로서 중등도의 증가를 보였고 만성간염, 간경화증, 미확정 만성간질환 및 폐쇄성 황달에 있어서는 중등도의 증가를 보였고 이들 LDH활성도의 증가는 주로 LDH_5의 증가에 인한것이 특징적이었다. 빈혈에 있어서 LDH활성도는 746.7±155.65U/L로서 이의 증가는 LDH_3, LDH_4 및 LDH_5의 증가에 인한 것이었다. 백혈병에 있어서는 1119.3±126.69 U/L 정도의 고도의 증가를 보였으며 이의 증가는 LDH_3, LDH_4 및 LDH_5의 증가에 의하였다. 호흡기질환에 있어 폐결핵은 LDH활성도가 740.0±117.63 U/L로서 증가하였으며 이의 증가는 LDH_4와 LDH_5의 증가에 의하였다. CO중독, Reye's syrdrome 및 asphyxia neonatorum은 다 같이 1619.0+516.05 U/L까지 고도의 증가를 보였고 이들 증가는 CO중독에서는 LDH_1과 LDH_5의 증가, Reye's syndrome에서는 LDH_4와 LDH_5의 증가, 그리고 asphyxia neonatorum에 있어서는 LDH_3 LDH_4 및 LDH_5의 증가에 의하였다. 당뇨병에 있어서 LDH활성도는 경도의 증가경향을 보였고 LDH_3, LDH_4 및 LDH_5의 증가가 원인이었다. 일반질환에 있어서 LDH활성도는 675.2±44.98 U/L이었고 LDH_3, LDH_4 및 LDH_5의 증가원인이었다. 악성종양에 있어서 LDH활성도는 962.0±248.50 U/L까지 저명한 증가를 보였고 이들 증가의 원인은 LDH_3, LDH_4 및 LDH_5의 증가에 인한 것이었고 위암과 원발성간암에서 가장 심하였다. The serum LDH activities and LDH isoenzyme patterns were determined in order to evaluate the clinical significances in various diseases presumed to be associated with LDH isoenzyme. Totally 359 cases were investigated for the patients on the LDH isoenzyme test request. In cardiovascular disorders, myocardial infarction showed high increase of LDH activity as 1023.0±132.73 U/L and LDH_1 isoenzyme increase was the main cause. Congestive heart failure showed moderate increase of LDH activity as 650.3±42.3 U/L and the increases of LDH_4 and LDH_5 were the cause. In hepatobiliary disorders acute hepatitis showed moderate increase of LDH activity as 769.75±74.39 U/L and the cause was mainly LDH_5 increase. Chronic hepatitis, chronic liver disease and obstructive jaundice showed similar tendency and the same cause and the degrees were less prominent. In hematologic disorders anema showed moderate increase of LDH activity as 746.7±155.65 U/L and leukemia showed high increase of LDH activity as 1119.3±126.69 U/L and these elevations were due to LDH_3, LDH_4 and LDH_5. In respiratory disorders, pulmonary tuberculosis showed moderate increase of LDH activity as 740.0±117.63 U/L and this increase was due to LDH_4 and LDH_5. CO intoxication, Reye's syndrome and asphyxia neonatorum showed very high increase of LDH activity up to 1619.0±516.05 U/L and the causes were LDH_1 and LDH_5, LDH_4 and LDH_5, and LDH_3, LDH_4 and LDH_5 respectively. In diabetes mellitus a moderate increase of LDH activity was abserved as 675.2±44.98 U/L and it was from LDH_3, LDH_4 and LDH_5 increases. In malignant tumors, LDH activity was increased as high as 962.0±248.50 U/L, and the cause was from LDH_3, LDH_4, and LDH_5 increases. And the most prominent increase was found in stomach Ca.

조류 및 포유류 내 미토콘드리아 젖산탈수소효소 동위효소들의 분포와 역할

조성규(Sung Kyu Cho),염정주(Jung Joo Yum) 한국생명과학회 2017 생명과학회지 Vol.27 No.5

조류 및 포유류에서 미토콘드리아를 분리하고 모노아민산화효소(EC 1.4.3.4, monoamine oxidase)의 활성을 측 정하여 미토콘드리아의 분리도를 확인하였다. 미토콘드리아 각 분획에서 젖산탈수소효소(EC 1.1.1.27, lactate dehydrogenase, LDH)의 발현도를 생화학적 및 면역화학적 방법으로 분석하였다. 조류에 비하여 포유류에서는 미토콘드리아 LDH의 활성이 낮은 것으로 나타났다. 따라서 미토콘드리아 LDH의 역할은 포유류보다 조류에서 중요시된다고 생각된다. 조류 및 포유류 모든 조직의 단백질양은 세포기질보다 미토콘드리아에서 적게 나타났다. 생쥐와 골든햄스터의 세포기질에서는 LDH A4, A3B, A2B2, AB3 및 B4 다섯 가지 동위효소 이외에 testis-specific LDH C4 동위효소가 정소 조직에서 나타났다. 닭의 미토콘드리아 내에서는 AB hybrid형 동위효소 한 종류가 나타났다. 포유류에서 미토콘드리아 LDH 동위효소들은 조직 별로 다르게 나타났다. 생쥐의 미토콘드리아 내에서는 LDH A4 및 testis-specific C4 동위효소가 나타났다. 미토콘드리아 testis-specific LDH C4 동위효소는 생쥐에서만 나타났다. 골든햄스터 미토콘드리아 내에서는 LDH B4 동위효소가 lactate oxidase로서 작용하였다. 조류 및 포유류의 대사조건 및 서식환경에 따라 미토콘드리아 LDH 동위효소의 역할도 구분되는 것으로 생각된다. Mitochondria were isolated from bird and mammals. The activity of monoamine oxidase (EC 1.4.3.4) was then measured to identify mitochondrial isolation. Lactate dehydrogenase (EC 1.1.1.27, lactate dehydrogenase, LDH) isozymes in mitochondrial fractions were analyzed by biochemical and immunochemical methods. The activity of mitochondrial LDH was lower in mammals than in bird. Therefore, the role of mitochondrial LDH seems to be more important in bird than in mammals. The concentration of protein in all tissues of bird and mammals was less in the mitochondria than in the cytosol. In the cytosol of mice and golden hamsters, testis-specific LDH C4 isozyme was expressed in testis in addition to the LDH A4, A3B, A2B2, AB3, and B4 isozymes. A single LDH AB hybrid isozyme was expressed in the chicken mitochondria. In mammals, mitochondrial LDH isozymes were differed according to tissues. LDH A4 and testis-specific LDH C4 isozymes were expressed in the mitochondria of mice. The mitochondrial testis-specific LDH C4 isozyme was expressed only in the mice. In the golden hamster mitochondria, the LDH B4 isozyme functioned as a lactate oxidase. As our results show, the mitochondrial LDH seemed to be playing the different role in the bird and mammals in relation with their metabolic conditions and habitats.

기아상태에서 Ldh-C가 발현된 생쥐(Mus musculus) 조직의 젖산탈수소효소의 대사

염정주(Jung Joo Yum),김규동(Gyu Dong Kim) 한국생명과학회 2018 생명과학회지 Vol.28 No.1

젖산탈수소효소(Lactate dehydrogenase, EC 1.1.1.27, LDH)의 기능을 확인하기 위해서 Ldh testis-specific C가 발현된 생쥐(Mus musculus)를 48 hr과 96 hr 기아상태로 유지시킨 후 조직들의 LDH 대사를 LDH 활성, 역학 및 동위효소를 분석하여 연구하였다. 골격근, 간 및 눈조직에서 LDH와 LDH A₄활성이 증가되어 혐기적 대사가 우세하였고, 심장과 신장조직의 LDH 활성은 감소되지만 LDH B₄ 활성이 증가되어 피루브산을 생성하는 호기적 대사가 우세하였다. 하지만 정소조직에서는 LDH C₄가 감소되었고, 뇌조직의 LDH 활성은 조직 중에서 가장 많이 증가되었지만 동위효소의 변화가 작고 피루브산의 양이 감소되었다. 신장조직을 제외한 조직들에서 Km<SUP>PYR</SUP>이 증가되어 피루브산에 대한 친화력이 감소된 것으로 확인되었다. 실험결과 Ldh-A, B가 발현된 조직에서는 상대 농도가 큰 동위효소의 활성이 증가되었으나 Ldh-A, B, C가 발현된 정소조직은 LDH C₄가 감소되어 기능이 저하되었으며 특히 뇌조직에서 LDH는 피루브산 환원효소로서 역할을 하는 것으로 확인되었다. 따라서 이 과정은 기아상태에서 에너지를 생성하는 기작이 될 수 있는 것으로 사료된다. To confirm the function of lactate dehydrogenase (LDH) (EC 1.1.1.27, LDH), its metabolism was studied by activity, kinetics, and isozyme analysis in tissues of Ldh testis-specific C expressing mice (Mus musculus) maintained in a state of starvation for 48 hr and 96 hr. In skeletal muscle, liver, and eye tissues, LDH and LDH A₄ activity increased and anaerobic metabolism predominated. While LDH activity in the heart and kidney tissues decreased, LDH B4 activity increased and aerobic metabolism predominated, producing pyruvic acid. In the testis tissue, LDH C₄ activity decreased. In the brain tissue, LDH activity increased, but the isozyme change was small and the amount of pyruvic acid decreased. Km<SUP>PYR</SUP> increased in tissues other than kidney tissue, and the affinity for pyruvic acid decreased. Consequently, in Ldh-A and B-expressing tissues, the activities of isozymes with higher concentrations increased. However, in Ldh-A, B, and C-expressing tissue, C₄ decreased and the function of the tissue also decreased. In particular, LDH in brain tissue played a role as a pyruvate reductase. Therefore, this process might be the mechanism for producing energy in the state of starvation.

뱀장어의 신장과 혈청의 LDH Isozyme에 미치는 파라티온과 인삼의 영향

김수영,이춘구,배인화 숙명여자대학교 자연과학연구소 1997 자연과학논문집 Vol.- No.8

뱀장어의 신장과 혈청의 LDH isozyme에 미치는 파라티온과 인삼의 영향을 흡광광도측정법과 전기영동법으로 연구하였다. 대조군과 비교할 때 파라티온 처리군의 신장 총 LDH활성은 눈에 띄게 감소되고 혈청의 활성은 증가되었다. 파라티온-인삼군에서는 신장과 혈청의 총 LDH활성이 대조군의 수치와 유사하였다. 파라티온 처리 후 신장의 LDH₁분획의 활성은 감소되고 LDH5의 활성은 증가된 반면 혈청의 LDH₁과 LDH5 분획의 활성은 증가되었다. The effects of parathion and ginseng on the LDH isozymes of kidney and serum in eel were investigated by means of spectrophotometry and electrophoresis. After exposure to parathion, the total LDH activity was remarkably decreased in the kidney and increased in the serum as compared with those of control group. The levels of total activities in the kidney and serum of parathion-ginseng group were similar to those of the control. In the kidney, the enzyme activity of LDH₁ fraction was decreased and that of LDH5 was increased by parathion. In the serum, on the other hand, the enzyme activities of LDH₁ and LDH5 fractions were increased after parathion exposure.

國內詞育 원숭이의 血淸 LDH의 總活性値와 isoenzyme에 관한 硏究

윤상보,김덕환,서지민,신남식,현병화,김명철,윤효인,박배근,송희종 한국임상수의학회 2001 한국임상수의학회지 Vol.18 No.4

Non-human primates have been increasing in demand as important experimental animals and companion animals, domestically and internationally. The number of non-human primates for these purposes will be much enhanced in the near future. Despite this trend, basic physiological data are scarcely available in these animal species, leading to the difficulty to diagnose diseases when necessary, due to the absence of reference values. Particularly, there is not any report on the total activity of LDH of non-human primates, let alone LDH isoenzyme patterns, in Korea. LDH isoenzymes have a high level of efficaciousness as diagnostic and prognostic aids in various diseases. In this study, total activities and isoenzyme patterns of LDH were measured to obtain their reference values in domestically reared common marmosets, crab-eating macaques and Japanese macaques. There were widespread different values of serum total LDH among the non-human primate species experimented in this study. Serum LDH values of common marmosets and crab-eating macaques were 597.5$\pm$243.1 IU/l and 605.3$\pm$312.6 IU/l, respectively, whereas those of Japanese macaque showed 1,209$\pm$473.8 IU/l. Five isoenzyme fractions of LDH were observed in all experimented non-human primates but their ranks and proportions represented different patterns one another. In common marmosets, the percent of fraction for serum LDH1, LDH$_2$, LDH$_3$, LDH$_4$, and LDH$_{5}$ was 13.7$\pm$6.4%, 23.3$\pm$3.6%, 29.2$\pm$5.0%, 9.4$\pm$1.4% and 24.4$\pm$7.5%, respectively. The rank of LDH isoenzymes was LDH$_3$>LDH$_{5}$>LDH$_2$>LDH$_1$>LDH$_4$, in the descending order. For crab-eating macaques, the fraction of serum LDH$_1$, LDH$_2$, LDH$_3$, LDH$_4$, and LDH$_{5}$ occupied 19.5$\pm$12.7%, 25.3$\pm$9.3%, 23.8$\pm$8.1%, 10.2$\pm$2.8% and 21.3$\pm$14.2%, respectively. The order of LDH isoenzymes was LDH$_2$>LDH$_3$>LDH$_{5}$>LDH$_1$>LDH$_4$, from top to down. On the while, in Japanese macaques, the fraction of serum LDH$_1$ to LDH$_{5}$ showed 23.4$\pm$11.8%, 30.5$\pm$4.1%, 17.4$\pm$3.9%, 11.3$\pm$3.7% and 13.8$\pm$5.6%, respectively. The decreasing order indicated LDH$_2$>LDH$_1$>LDH$_3$>LDH$_{5}$>LDH$_4$. In conclusion, values such as LDH and LDH isoenzyme patterns of investigated for the first time from non-human primates reaared in Korea, could be reference values for the optimal diagnosis and therapy of diseases of the corresponding animal species. Other parameters of hematology and blood biochemistry are urgently needed to study for the benefit of our intimate non-human primates.an primates.

급성 담석성 및 알콜성 췌장염에서 췌장괴사의 지표로써 LDH/AST비의 유용성

김정한(Jung Han Kim),김창억(Chang Uck Kim),장형주(Hyun Joo Jang),박원종(Won Jong Bahk),박진철(Jin Cheol Park),강승식(Seung Sick Kang),계세협(Sea Hyub Kae),이진(Jin Lee),김용범(Yong Bum Kim),주상언(Sang Aun Joo) 대한내과학회 2000 대한내과학회지 Vol.58 No.2

Background : Lactate dehydrogenase (LDH) has been reported to be a sensitive indicator of pancreatic necrosis (PN), and some studies suggested that an elevation of the ratio of LDH to AST (LDH/AST ratio) woud be more accurate indicator of PN in acute biliary pncreatitis (BP). However, there were no studies in alcoholic pancreatitis (AP). The aim of this study was to assess the clinical usefulness of LDH/AST ratio in alcoholic pancreatitis (AP) as a indicator of PN. Methods : On the basis of CT scan findings, the patients were categorized into two groups as having PN or non-PN. The plasma levels of the LDH, AST and LDH/AST ratio over two weeks postadmission period were evaluated and compared with in two groups of patients with BP (consiting of 12 PN and 34 non-PN patients), and with AP (consisting of 14 PN and 38 non-PN patients). Results : In acute BP, on post-admission days 1 and 2, the LDH/AST ratio were low in both groups without significant difference. In the group with PN, thereafter, the LDH/AST ratio increased gradually, reached peak values at the 7th days and decreased. In the non-PN patients, the LDH/AST ratio increased gradually, but remained below the control range. The LDH/AST ratios were significantly higher from post-admission day 3 in the group with PN than in the non-PN group. In acute AP, the LDH levels were significantly higher over two weeks from admission day in the PN patients. The LDH/AST ratios were remained within or below the control range in both groups, though with statistically significnat difference. Conclusion : The LDH/AST ratio could be used as an indicator of PN in acute BP. In acute AP, however, LDH was a more useful indicator from the early stage in the course. (Korean J Med 58:161-169, 2000)