저수온, 적수온, 고수온에서의 미성어기 조피볼락(Sebastes schlegeli)의 배합사료 적정 공급률

김민기,이초롱,김유정,오대한,이봉주,김성삼,이경준 한국수산과학회 2015 한국수산과학회지 Vol.48 No.3

Three feeding trials were conducted to investigate the optimal feeding rates of subadult Korean rockfish Sebastes schlegeli at low, optimal and high water temperatures. In Exp-I, triplicate groups of fish (248 g) were fed a commercial diet (CD) with five different feeding rates of 0.1%, 0.2%, 0.4%, 0.6% and 0.8% body weight per day for 10 weeks at the low temperature (11.6-15.2°C). In Exp-II, fish (129 g) were fed the same CD with five different feeding rates of 0.4%, 0.8%, 1.2%, 1.6% and 2.0% body weight per day for 10 weeks at the optimal temperature (16.0-18.8°C). In Exp-III, fish (85.7 g) were fed the same CD with five different feeding rates of 0.4%, 0.8%, 1.2%, 1.6% and 2.0% body weight per day for 12 weeks at the high temperature (21.0-28.5°C). The optimal feeding rates in the low, optimal, and high water temperatures were estimated to be 0.56%, 0.93% and 1.17% body weight per day, respectively, for subadult Korean rockfish.

사료내 김(Porphyra)과 다시마(Laminaria japonica) 첨가가 조피볼락(Sebastes schlegeli) 치어의 성장, 사료 이용성, 체조성 및 혈액성상에 미치는 영향

전규호,조성환,이상민,남택정,김동수 한국수산과학회 2013 한국수산과학회지 Vol.46 No.5

This study examined the effects of the dietary inclusion of Porphyra and sea tangle Laminaria japonica on the growth, feed utilization, body composition, and plasma chemistry of juvenile Korean rockfish Sebastes schlegeli. Eight hundred and forty juvenile fish averaging 5.0 g were allocated 40 fish per tank to 21 180-L flow-through tanks. Seven experimental diets were prepared: control (Con) without additive, 0.5and 1% Porphyra extract (PE), 3% Porphyra powder (PP), 0.5 and 1% sea tangle extract (STE) and 3% sea tangle powder (STP), referred to as PE-0.5, PE-1, PP-3, STE-0.5, STE-1, and STP-3, respectively. Each additive was included in the experimental diet at the expense of the same amount of wheat flour. Each experimental diet was fed to triplicate groups of fish. The experimental diets had no effect on the survival,weight gain or specific growth rate of the fish, feed consumption, feed efficiency ratio, protein efficiency ratio, protein retention, hepatosomatic index, condition factor, moisture or crude protein content of the entire body excluding the liver or moisture, crude protein or crude lipid content of the liver. None of the plasma parameters were affected by the experimental diets. Based on these results, the dietary inclusion of Porphyra and sea tangle did not affect the growth, feed utilization, body composition or plasma chemistry of juvenile Korean rockfish.

Effects of Feeding Rate and Water Temperature on Growth and Body Composition of Juvenile Korean Rockfish, Sebastes schlegeli (Hilgendorf 1880)

Mizanur, Rahman Md.,Yun, Hyeonho,Moniruzzaman, M.,Ferreira, F.,Kim, Kang-Woong,Bai, Sungchul C. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.5

Three feeding trials were conducted to evaluate the effects of feeding rate and water temperature on growth and body composition of juvenile Korean rockfish, Sebastes schlegeli rearing at 3 different water temperatures. A total of 270 fish (each experiment) individual body weight (BW) averaging $16{\pm}0.3g$ ($mean{\pm}SD$) were fed a commercial diet for 4 wk at $16^{\circ}C$, $20^{\circ}C$, and $24^{\circ}C$. At each temperature, triplicate tanks were assigned to one of 6 feeding rates: 1.5%, 2.5%, 2.8%, 3.1%, 3.4%, and satiation (3.7% BW/d) at $16^{\circ}C$, 1.9%, 2.9%, 3.2%, 3.5%, 3.8% and satiation (4.1% BW/d) at $20^{\circ}C$ and 1.7%, 2.7%, 3.0%, 3.3%, 3.6%, and satiation (3.9% BW/d) at $24^{\circ}C$ water temperature. Weight gains of fish in satiation and 3.4% groups at $16^{\circ}C$, in satiation and 3.8% groups at $20^{\circ}C$ and in satiation and 3.6% groups at $24^{\circ}C$ were significantly higher than those of fish in the other treatments (p<0.05). A broken line regression analysis of weight gain indicated that optimum feeding rates of juvenile Korean rockfish were 3.41% at $16^{\circ}C$, 3.75% at $20^{\circ}C$ and 3.34% at $24^{\circ}C$ water temperature. Results of the present study indicate that the optimum feeding rate could be >3.1% but <3.41% at $16^{\circ}C$, >3.5% but <3.75% at $20^{\circ}C$ and >3.0% but <3.34% at $24^{\circ}C$. As we expected results suggest that fish performed better at $20^{\circ}C$ than $16^{\circ}C$ or $24^{\circ}C$ water temperature and the optimum feeding rate could be 3.1% BW/d to 3.7% BW/d in 16 g of juvenile Korean rockfish.

수온별 저산소 노출에 따른 조피볼락 (Sebastes schlgeli)의 혈액성상 변화

지보영,도용현,민병화,박미선,황형규,명정인,조재권 한국환경생물학회 2015 환경생물 : 환경생물학회지 Vol.33 No.4

The objective of this study was to evaluate the effects of acute hypoxia on the physiological stress responses (plasma cortisol as the primary response, and hematocrit, hemoglobin, plasma glucose, sodium, chloride, osmolality, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) as the secondary responses) of Korean rockfish. The mortality in Korean rockfish started at 0.7 mg L-1 and 0.8 mg L-1 levels at 12℃ and 20℃, respectively. And the time required for the mortality in experimental fish was 274 and 148 minutes at 12℃ and 20℃, respectively. Levels of hematocrit, hemoglobin, AST, ALT, ion concentration, osmolality, glucose and cortisol had significantly increased with decreasing dissolved oxygen at 12℃ and 20℃.

수온별 저산소 노출에 따른 조피볼락 (Sebastes schlgeli)의 혈액성상 변화

지보영,도용현,민병화,박미선,황형규,명정인,조재권 한국환경생물학회 2015 환경생물 : 환경생물학회지 Vol.33 No.4

The objective of this study was to evaluate the effects of acute hypoxia on the physiological stress responses (plasma cortisol as the primary response, and hematocrit, hemoglobin, plasma glucose, sodium, chloride, osmolality, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) as the secondary responses) of Korean rockfish. The mortality in Korean rockfish started at 0.7 mg L-1 and 0.8 mg L-1 levels at 12℃ and 20℃, respectively. And the time required for the mortality in experimental fish was 274 and 148 minutes at 12℃ and 20℃, respectively. Levels of hematocrit, hemoglobin, AST, ALT, ion concentration, osmolality, glucose and cortisol had significantly increased with decreasing dissolved oxygen at 12℃ and 20℃.

소음 스트레스가 조피볼락, Sebastes schlegeli의 cortisol과 glucocorticoid receptor의 발현에 미치는 영향

심민경 ( Min Kyung Shim ),한경남 ( Kyung Nam Han ) 한국어류학회 2008 韓國魚類學會誌 Vol.20 No.3

치어기 조피볼락 사료내 구기자 첨가효과

임동규,유광열,신동건,김종응,배준영,배승철,이정열 한국수산과학회 2009 한국수산과학회지 Vol.42 No.3

A 8-week feeding trial was conducted to investigate the effects of dietary supplementation of Kugija (Lycium chinense) on the growth and immunological response in juvenile Korean rockfish (Sebastes schlegeli). Six experimental diets were supplemented with Kugija at 0, 0.1, 0.5 1.0, 3.0 and 5.0% (K0, K0.1, K0.5, K1.0, K3.0, K5.0) on a dry-matter basis. After 2 weeks, triplicate groups of 30 fish initially averaging 3.36±0.2 g (mean±SD) were randomly distributed into the aquarium and were fed one of the experimental diets for 8 weeks. By the end of the 8-week feeding trial, fish fed the K0.5 and K1.0 diets exhibited a higher weight gain and specific growth rate than fish fed K0 and K0.1 diets (P<0.05). Feed efficiency of fish fed the K0.5 diet showed significant higher value than that of fish fed the K0, K3.0 and K5.0 diets (P<0.05). Hepatosomatic index of fish fed the K5.0 diet was significantly higher than that of fish fed the K0.1 and K5.0 diets (P<0.05). Hematocrit of fish fed the K0.5, K1.0 and K5.0 diets was significantly higher than that of fish fed the K0 diet (P<0.05). Glutamic oxaloacetic transaminase of fish fed the K0.5, K1.0, K3.0 and K5.0 diets was significantly lower than in fish fed the other diets (P<0.05). Glutamic pyruvic transaminase activity of fish fed the K3.0 diet was significantly lower than those of fish fed the K0 and K0.1 diets (P<0.05). However, there was no significant difference in the activity of the transaminase in fish fed the K0.5 K1.0, K3.0 and K5.0 diets. Lysozyme activity of fish fed the K0.5 and K1.0 diets was significantly higher than that in fish fed the K0 and K0.1 diets (P<0.05). Respiratory burst activity of fish fed the K3.0 diet was significantly higher than those of fish fed the K0 and K0.1 diets (P<0.05). However, there was no significant difference in respiratory burst activity of fish fed the K0.5 K1.0, K3.0 and K5.0 diets. Fish fed Kugija showed lower early mortality than fish fed a Kugija-free diet upon challenge with Vibrio ordalii. The results suggest that feeding of Kugija (Lycium chinense) enhances growth, non-specific immunity and disease resistance in juvenile Korean rockfish.

A Review of the Optimum Feeding Rates and Feeding Frequency in Korean Rockfish Sebastes schlegeli Reared at Seven Different Water Temperatures

Mizanur, Rahman M.,Bai, Sungchul C. The Korean Society of Fisheries and Aquatic Scienc 2014 Fisheries and Aquatic Sciences Vol.17 No.2

Seven feeding trials were conducted to determine the effects of feeding rate and frequency in the Korean rockfish Sebastes schlegeli at seven different water temperatures. Two feeding-rate experiments for 5-g Korean rockfish at $17^{\circ}C$ and $20^{\circ}C$; three feeding rate experiments for 16-g Korean rockfish at $16^{\circ}C$ and $24^{\circ}C$; and finally, two feeding frequency experiments for 93- and 100-g at $15^{\circ}C$ and $20^{\circ}C$ were conducted. Twenty fish averaging $5.5{\pm}0.2$ g ($mean{\pm}SD$) were randomly distributed into 18 indoor tanks containing 40-L seawater from a semi-recirculation system. Fish were fed a commercial diet for 4 weeks at water temperatures of $17^{\circ}C$ and $20^{\circ}C$. Two feeding trials were conducted to determine the optimum feeding frequency in growing Korean rockfish reared at temperatures of $15^{\circ}C$ and $19^{\circ}C$. Broken line regression analysis of weight gain (WG) indicated that the optimum feeding rates of 5-g growing Korean rockfish were 4.48% (BW/day; BW, body weight) at $17^{\circ}C$ and 4.83% (BW/day) at $20^{\circ}C$. Broken line regression analysis of WG showed optimum feeding rates of 16-g juvenile Korean rockfish of 3.41% (BW/day) at $16^{\circ}C$, 3.75% (BW/day) at $20^{\circ}C$, and 3.34% (BW/day) at $24^{\circ}C$. The biological performance, results, along with morphological indices and serological characteristics, suggest that a feeding frequency of 1 meal/day was optimal to improve WG in growing Korean rockfish grown from 93 to 133 g at a water temperature of $15^{\circ}C$, and 100 to 132 g at $19^{\circ}C$.

A Review of the Optimum Feeding Rates and Feeding Frequency in Korean Rockfish Sebastes schlegeli Reared at Seven Different Water Temperatures

Rahman M Mizanur,배승철 한국수산과학회 2014 Fisheries and Aquatic Sciences Vol.17 No.2

Seven feeding trials were conducted to determine the effects of feeding rate and frequency in the Korean rockfish Sebastes schlegeli at seven different water temperatures. Two feeding-rate experiments for 5-g Korean rockfish at 17°C and 20°C; three feeding rate experiments for 16-g Korean rockfish at 16°C and 24°C; and finally, two feeding frequency experiments for 93- and 100-g at 15°C and 20°C were conducted. Twenty fish averaging 5.5 ± 0.2 g (mean ± SD) were randomly distributed into 18 indoor tanks containing 40-L seawater from a semi-recirculation system. Fish were fed a commercial diet for 4 weeks at water temperatures of 17°C and 20°C. Two feeding trials were conducted to determine the optimum feeding frequency in growing Korean rockfish reared at temperatures of 15°C and 19°C. Broken line regression analysis of weight gain (WG) indicated that the optimum feeding rates of 5-g growing Korean rockfish were 4.48% (BW/day; BW, body weight) at 17°C and 4.83% (BW/day) at 20°C. Broken line regression analysis of WG showed optimum feeding rates of 16-g juvenile Korean rockfish of 3.41% (BW/day) at 16°C, 3.75% (BW/day) at 20°C, and 3.34% (BW/day) at 24°C. The biological performance, results, along with morphological indices and serological characteristics, suggest that a feeding frequency of 1 meal/day was optimal to improve WG in growing Korean rockfish grown from 93 to 133 g at a water temperature of 15°C, and 100 to 132 g at 19°C.

암모니아 노출에 따른 조피볼락(Sebastes schlegeli)의 생리학적 반응

민병화 ( Byung Hwa Min ),박미선 ( Mi Seon Park ),신윤경 ( Yun Kyung Shin ),도용현 ( Yong Hyun Do ),명정인 ( Jeong-in Myeong ) 한국환경생물학회 2014 환경생물 : 환경생물학회지 Vol.32 No.4

본 연구에서는 암모니아 노출에 따른 조피볼락의 생리학적 반응을 조사하고자 아가미 Na+/K+-ATPase (NKA)활성을 비롯한 혈장 parameters를 분석하였다. 실험구의 암모니아 농도는 대조구(자연해수), 1, 2, 4, 8 mg L-1였으며, 조피볼락을 각각의 실험구에 3시간동안 노출한 다음혈액 및 아가미 조직을 샘플링하였다. 실험구의 암모니아 농도가 높아질수록 혈장 암모니아 농도가 증가하였으며, 아가미 NKA 활성 또한 증가하는 경향을 보였다. 혈장 Cl-를 제외한 외부의 암모니아 영향을 받지 않았으나, 4, 8 mg L-1구의 Na+, K+ 및 삼투질농도는 대조구및 1, 2 mg L-1구보다 유의하게 높았다. 암모니아 노출에 따른 조피볼락의 혈장 코티졸은 암모니아 농도와 선형관계를 보였으며, 혈장 글루코스 또한 코티졸과 동반상승하는 것으로 나타났다. 1, 2 mg L-1구의 hematocrit는 대조구와 차이를 보이지 않았으나, 4, 8 mg L-1구는 나머지 실험구보다 유의하게 높았다. 암모니아 농도가 높을수록 아가미 조직 손상은 심하였으며, 특히 4, 8 mg L-1구에서는 상피세포의 과증식, 분리, 괴사 및 2차새변의 곤봉화(club-shaped lamella) 현상이 관찰되었다. The aim of the present study was to assess the effects of ammonia on physiological responses in Korean rockfish (Sebastes schlegeli). Interestingly, no mortality were observed when the specimens (301.1±8.0 g) were exposed to five levels of un-ionized ammonia (NH3) (control, 1, 2, 4,8 mg L-1) for 3 hours. Furthermore, a significantly higher increase in gill Na+/K+-ATPase (NKA) pump activity with was detected due to the ammonia exposure. The activity of the fishes were found to be 4 and 8 mg L-1 NH3, which was significantly high compared to normal 1 mg L-1 NH3. Although ammonia exposure had no effect on plasma Cl-, exposure to both 4 and 8 mg L-1 NH3, however it led to increase in the plasma Na+, K+ and osmolality levels. Also, prolong ammonia exposure cause increase of plasma cortisol and glucose levels. The increase in glucose was accompanied by an increase in cortisol. The fish exposed to 4 and 8 mg L-1 NH3 showed significantly higher hematocrit than control group than those exposed to 1 and 2 mg L-1 NH3. The intensity of cell damage increased with the increase concentration and exposure to ammonia. Furthermore, hyperplasia, separation and epithelial necrosis were also observed in gill tissues. Taken together, the results showed that direction of changes to the investigated parameters can be used to determine the physiological responses of Korean rockfish to ammonia.