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국내분리 porcine reproductive and respiratory syndrome(PRRS) virus의 특성에 관한 연구
장경수,권창희,전무형 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-
The Korean isolates of porcine reproductive and respiratory syndrom(PRRS) virus, KPRRSV-1 and KPRRSV-2, were characterized on the aspects of sensitivity on physicochemical treatment, typing of nucleic acid, hemagglutinating activity, replication in cultured cells and morphology in electron microscopy. The isolates were labile to ether or chloroform, acid(pH 3.0) or alkali(pH 8.0), and heating(56℃, 30min). The viruses showed no haemagglutinating activity, and inhibition of replication in the medium containing 5-bromo-2'-deoxyuridine. Virus replication was more active in porcine alveolar macrophage(PAM) as compared with that in MARC-145 cell line, showing the maximum titer of 10^4.6TCID_50/㎖ at 5 days post-exposure(pe). Cytopathic effects(CPE) were observed more evidently in the PAM cells than in MARC-145 cell line. In electron microscopy, observation for the isolates showed oval or spherical types of virions, surrounded by a thin membrane-like envelope. The virions were from 45 to 86nm in diameter containing the cores of 35∼50nm in size
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국내분리 porcine reproductive and respiratory syndrome(PRRS) virus에 인공감염된 자돈에 관한 연구
장경수,조성환,권창희,전무형 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-
The Korean isolates of porcine reproductive and respiratory syndrom(PRRS) virus, KPRRSV-1 and KPRRSV-2, were studied on the aspects of pathogenicity in the experimentally infected piglets. The piglets inoculated intranasally(in) with the virus at 10^4.5TCID_50/㎖(GroupⅠ) showed clinical signs of milder fever, respiratory disorders, anorexia, growth retardation and transient leucopenia. It was evident that the virus could be transmitted by contract. Histopathologically interstitial pneumonitis was markedly noted in the lung of all infected piglets. In studies on antibody responses of the experimental animals, seroconversions were noted at 6∼9 days pe in GroupⅠ(in inoculated) and at 12∼15 days pe in Group Ⅱ(contract). The antibody titers at 30 days pe were ranged from 256 to over 1024. By avidin-biotin immunofluorescence(ABIF) assay, the PRRS virus antigens were detected in bronchi, bronchiol and alveolar epitheliums and interalveolar septa of the experimentally infected piglets. The viruses were recovered from the tissues of lung and spleen of the infected piglets.
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