Lactobacillus plantarum과 Saccharomyces cerevisiae의 상호작용이 채소발효음료에 미치는 영향

김혜자,양차범 漢陽大學校 韓國生活科學硏究所 1994 韓國 生活 科學 硏究 Vol.- No.12

값싼 채소류를 원료로 젖산발효 음료를 제조하기 위하여 선별된 젖산균과 효모의 혼합발효의 가능성을 검토하였다. 채소즙의 발효에 사용될 균주로서 젖산균은 L. palantarum KCTC 3797와 효모균주로는 낮은 pH에서 생육하면서 생육상태가 좋고 향미가 우수한 Saccharomyces sp. J2-b6와 분양균주인 S. cerevisiae KCTC 7193이 혼합발효에 가장 적합하였다. L. palantarum KCTC 3099, S. cerevisiae KCTC 7193, Saccharomyces sp. J2-b6의 최종산도는 각각 1.17%, 0.42%, 0.39%로 다른 분리균주들에 비해 높았으며, L. plantarum KCTC 3099의 lactic acid 생성은 151mM, ethanol 생성은 S. cerevisiae KCTC 7193이 76mM. Saccharomyces sp. J2-b6가 91mM로 나타났다. S. cerevisiae KCTC 7193, Saccharomyces sp. J2-b6를 L. palantarum KCTC 3099와 각각 혼합발효 시킴으로써 L. palantarum KCTC 3009의 단독발효(pH 3.10, 총산도 1.17%)에 비해 최종 pH는 3.57, 4.01로 다소 높아졌으며 최종 산도도 0.75%, 0.58%로 감소되었다. S. cerevisiae KCTC 7193의 접종비율을 다양하게 함으로써 lactic acid와 ethanol의 생성비를 조절할 수 있었고 동시에 30℃에서 7일 이내에 모든 당을 발효시킬 수 있었다. 이때 S. cervisiae KCTC 7193의 접종량을 증가시켰을 때 lactic acid의 생성은 감소한 반면 ethanol양은 증가되었으며 Saccharomyces sp. J2-b6와 L. palantarum KCTC 3099의 혼합발효에서도 유사한 양상을 나타내었다. 또한 접종시간을 달리 함으로써 lactic acid와 ethanol의 최종 생성비를 조절할 수 있었는데 효모를 젖산균보다 24시간 먼저 접종하였을 때가 동시에 접종한 경우나 젖산균을 효모보다 24시간 먼저 접종한 경우보다 ethanol과 lactic acid의 생성비가 훨씬 더 크게 나타났다. Lactobacillus plantarum and yeasts were isolation by screening method from vegetables as carbon sources to increase the effects of mixed culture fermentations. Selected yeast strain which was designated as Saccharomyces sp. J2-b6 grew well at low pH level and produced suitable ethanol with good flavor. Among several yeast strains, Saccharomuces sp. J2-b6 and S. cerevisiae KCTC 7193 were suitable for mixed culture fermentations with L. plantarum KCTC 3099. The amounts of acid produced by L. plantarum KCTC 3099, S. cerevisiae KCTC 7193 and Saccharomyces sp. J2-b6 was 1.17%, 0.42% and 0.39%, respectively. When each of S. cerevisiae KCTC 7193 and Saccharomyces sp. J2-b6 was added to L. plantarum KCTC 3099 independently, the final acidity were reduced to 0.75% and 0.58%, respectively, with a higher final pH(3.57, 4.01), compared to single L. plantarum KCTC 3099 culture (pH 3.10, total acidity 1.17%), By varying the inoculumn size of L. plantarum KCTC 3799 and yeasts in mixed culture, it was possible to manipulate the product ratios of lactic acid to ethanol and was able to ferment all of the sugar. Variation of inoculation time of S. cerevisiae KCTC 7193 and L. plantarum KCTC 3099 produced a significant effect on the end-product ratios. When inoculation of S. cerevisiae KCTC 7193 were preceded than that of L. plantarum KCTC 3099 by 24hrs, a greater proportion of ethanol to tactic acid was formed(lactic acid : ethanol = 99 : 33mM), compared to simultaneous inoculation (119 : 30mM) or inoculation of S. cerevisiae KCTC 7193 24hrs after L. plantarum KCTC 3099 (122 : 18mM). Also, a similar pat tern was observed when Saccharomyces sp. J2-b6 was added to L. plantarum KCTC, 3099 culture (59 : 45mM, 44 : 43mM. 117 : 15mM. respectively).

시설내 공기순환이 참외 발효과 발생에 미치는 영향

신용습,연일권,배수곤,최성국,최부술 (사) 한국생물환경조절학회 2001 생물환경조절학회지 Vol.10 No.1

This experiment was conducted to investigate the effect of air circulation and forced ventilation ofgreenhouse on the development of fermented fruits in an oriental melon. An air circulation systemand a forced ventilation system were operated during 09:30 17:00 at a 15 min. interval from Apr. 6, 5days after fruit setting, to Jun. 29, everyday except rainy days. Wind velocities in the greenhouse were0.06 0.08, 0.24 0.32, and 0.60 0.72 m s-1 in the naturally ventilated (control), in the air circulated, andin the forced ventilated treatment, respectively. No significant difference in plant growth parameters,including leaf length and width, were observed between treatments. However, the amount of xylemexudate increased in the air circulated treatment as compared to the control. Percent of fermentedfruits significantly decreased in the air circulated treatment as compared to the control. The forcedventilation treatment showed no significant difference in percent of fermented fruits as compared tothe control or to the air circulated treatment.

오이묘에 냉온 스트레스가 광합성 및 생리반응에 미치는 영향

남윤일,우영회 (사) 한국생물환경조절학회 2001 생물환경조절학회지 Vol.10 No.3

This study were conducted to investigate the influence of chilling on photosynthetic rate, root activ-ity, contents of total sugars and fatty acids of cucumber seedlings grown in a greenhouse. Even thoughphotosynthetic activity of seedlings exposed to 0oC for 5 hours was little or insignificantly influenced,it was reduced by 52.8% and 67.7% in seedlings exposed to the same temperature for an extended 10and 24 hours, respectively. Photosynthetic rate decreased significantly when seedlings were illumi-nated, as compared to continuously held under darkness, during 15 hours of chilling treatment at 3oC.Recovery of photosynthetic ability was also retarded by illumination during a recovery period afterchilling treatment. Root activity, as measured by the oxidation power of a-naphtylamine, was signifi-cantly reduced by chilling treatment at 0 to 6oC, but amount of bleeding xylem sap collected at 40days after chilling treatment was not significantly different among treatments. Total sugar contentincreased by 12 and 23% as compared to the control in seedlings chilled for 24 and 48 hours, respec-tively, at 3oC. Contents of unsaturated linolenic and oleic acids increased, while content of saturatedpalmitic acid decreased with chilling treatment.

상대습도가 황금배( (Pyrus pyrifolia Nakai cv.) 동녹발생에 미치는 영향

조일환,한점화,우영희,최장전,서흥수 (사) 한국생물환경조절학회 2002 시설원예‧식물공장 Vol.11 No.1

The occurrence rate of russet in hangkeumbaepear showed significant difference by yearsbecause the russet occurrence is greatly affected by the amount of rainfall. This study was conductedto analyse the relationship between rainfall and russet occurrence by artificial treatment of highhumidity. Under high relative humidity condition, stomatal resistance decreased and average fruitweight was higher since the increased net photosynthesis rate accumulation accelerated fruit growth.The russet occurrence began on July 25, when the growth speed of fruit weight and fruit surface is themost fast. Russet occurrence rate was higher in high relative humidity condition because the fruitgrowth was accelerated. Since the fruit calcium concentration change is extreme in late July, it isassumed that the deceased calcium content is related to the occurrence of russet in 'Whangkeumbae'pear. When the high relative humidity condition is maintained after rainfall, the amount of net photo-synthesis rate increase and fruit growth is accelerated. Therefore, the unbalance in the amounts oftransferred photosynthesis assimilation product, water and mineral elements would be one of the rea-sons for the russet occurrence in hangkeumbaepear.

양액재배용 팽연화 왕겨배지의 적정 사용기간

임상현,유근창,김경희,안문섭 (사) 한국생물환경조절학회 2001 시설원예‧식물공장 Vol.10 No.2

In an effort to evaluate the economic value and durability of the expanded rice hull as substrates,changes in the physical and chemical properties of material and plant growth in that substrate werestudied. Using an electron microscope, the structure of used and new expanded rice hull substrate wasexamined. Considerable decomposition was found in the substrate which had been used one to thretimes. Compactness and lowered porosity in the used substrates were probably caused by decomposi-tion. The results of cation analysis showed the possible destruction of cell wall of rice hulls. AbundantCa2+ in the substrates used for two to three times also indicated the possibility of decomposition. Intomato yield compariso, 15.2% more yield of tomato fruit in a new substrates indicated the negativeeffects of decomposition of one-time used substrates. Yield decreased in the substrates used for thretimes. If perlite substrates is used for three years before renewal and the cost of the perlite renewal iscounted, 65.3% saving in the cost will be realized with the use of an expanded rice hull substrate.Another positive effect of the expanded rice hull substrate is the decrease of environmental contami-nation.

대동맥궁 차단증을 동반한 동맥간의 일차 완전교정 1예 보고

성시찬,박준호,이형두,김시호,우종수,이영석 대한흉부외과학회 2003 Journal of Chest Surgery (J Chest Surg) Vol.36 No.10

대동맥궁 차단증을 동반한 동맥간은 매우 중한 자연경과를 갖고 있는 희귀한 선천성 심장 기형이다. 생후 25일, 체중 3.1 kg의 신생아에서 정중흉골절개술에 의한 일차 완전교정술을 시행하였다. A형 대동맥궁 차단증은 하행대동맥을 원위부 상행대동맥에 직접 문합하였으며, 폐동맥을 동맥간으로부터 분리하여 동맥간의 앞쪽에 위치한 다음 처리하지 않은 무판막 자가심낭도관을 사용하여 우심실 유출로를 재건하였다. 성공적 교정술 후 약 1년간의 양호한 추적결과를 경험하였기에 보고하는 바이다.