SBY-Ⅲ이 비만 및 비만 후 식이조절 흰쥐에 미치는 영향

우경하 ( Kyung Ha Woo ),정석희 ( Seok Hee Chung ),이종수 ( Jong Soo Lee ),김성수 ( Sung Soo Kim ),신현대 ( Hyun Dae Shin ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.2

Objectives : This experimental study was designed to investigate the effect of SBY-Ⅲ extract on the weight, cell size of epididymal fat-pad, fat accumulation area in liver, serum lipid level and UCP1 mRNA in brown adipose tissue of high fat diet-fed obese rats continued by high fat diet and regulated by normal Diet Methods : The body weight gain, weight of the internal organs(epididyrmis, liver, brown adipose tissue), insulin, triglyceride, total cholesterol, total lipid, free fatty acid, expression of UCP1 mRNA were measured in high fat diet-fed obese rats continued by high fat diet and regulated by normal diet. The experimental study are divided into exp-Ⅰand exp-Ⅱ. Each study was adminstered normal diet, high fat diet and SBY-Ⅲ according to each situation. Normal group is normal diet for 8 weeks. Exp-Ⅰ are divided into control group(high fat diet for 8 weeks) and sample group(high fat diet for 8 weeks and SBY-Ⅲ for last 2 weeks). Exp-Ⅱ are divived into control group(high fat diet for 6 weeks and normal diet for 2 weeks) and sample group(high fat diet for 6 weeks and normal diet with SBY-Ⅲ for 2 weeks). These were then compared mutually. Results : 1. Irrespective of diet control, sample group taken SBY-Ⅲ showed the more effective decrease of weight gain than control group and diet control-fed sample group with SBY-Ⅲ showed the more effective decrease of weight loss including weight gain than control group. 2. Irrespective of diet control, sample group taken SBY-Ⅲ showed the more effective decrease cell size of epididymal fat-pad, fat accumulation area in liver than control group. 3. Non diet control-fed sample group taken SBY-Ⅲ showed the more effective decrease of serum triglyceride, total lipid, free fatty acid than control group and diet control-fed sample group taken SBY-Ⅲ showed the decrease of serum triglyceride, free fatty acid than control group. 4. Only diet control-fed sample group taken SBY-Ⅲ showed the decrease of UCP1 volume. Conclusions : These results shows that SBY-Ⅲ has effects on anti-obesity, especially keeping pace with diet control.

Effect of moderate exercise training and low-fat diet on peritoneal macrophage immunocompetence in high-fat diet-induced obese mice model

( Wang Lok Lee ),( Jun Hyun Jeong ),( Hee Geun Park ),( Youn Gran Lee ),( Soon Mi Kwon ),( Ha Kyoung Jang ),( Jong Kui Jun ),( Myung Hwa Kim ),( Sang Ki Lee ),( Kwang Mu Lee ) 한국운동영양학회 2012 Physical Activity and Nutrition (Phys Act Nutr) Vol.16 No.3

This study investigated the effect of moderate exercise training on macrophage immunocompetence in high fat diet-induced obese mice. To determine the differential effects of exercise training and low fat diet on macrophage, C57BL/6 male mice (4 week old, n=32) were fed high-fat diet for 5 weeks to induce obese. The high fat-induced obese mice were then divided into four groups and differently treated by high/low fat diet and exercise training for a further 8 weeks; HHC (13 weeks high-fat diet), HHE (13 weeks high-fat diet with 8 weeks Exercise training) and HLC (5 weeks high-fat diet and 8 weeks low-fat diet), HLE (5 weeks high-fat diet and 8 weeks low-fat diet with exercise training). The exercise groups were performed on a motorized treadmill, running for 30-60 min/day at 10-22 m/min, 0% grade for 8wks. Body weight was significantly decreased for those with a low-fat diet and exercise training (HLE). Macrophage phagocytosis was significantly increased by low-fat diet and exercise training (HLE). The percentage of stimulation of NO release induced by LPS was higher in HLE than in other groups. The percentage of stimulation of IL-1β release induced by LPS was higher in HHE and HLE than in other groups. The percentage of stimulation of IFN-γ release induced by LPS was higher in HLE than in other groups. These findings suggest that moderate exercise and low-fat diet have beneficial effects on macrophage immunocompetence in high fat diet-induced obese mice.

고지방식이를 섭취한 성장기 쥐에서 꾸지뽕잎이 혈중 지질 농도 및 항산화 효소활성에 미치는 영향

박은혜(Eun-Hye Park),최미자(Mi-Ja Choi) 한국차학회 2020 한국차학회지 Vol.26 No.4

꾸지뽕잎이 고지방식이를 섭취한 성장기 수컷 쥐에서 혈중 지질 농도와 간 지질 함량 및 항산화 효소활성에 미치는 영향을 아래와 같이 요약하였다. 평균 식이섭취량은 고지방군이 대조군과 고지방+꾸지뽕군에 비하여 유의적으로 낮았고(p<0.05), 체중증가량도 유의적으로 낮았다(p<0.05). 식이효율은 고지방군, 고지방+꾸지뽕군이 대조군에 비하여 유의적으로 높았다(p<0.05). 혈중 총 콜레스테롤, LDL-콜레스테롤은 고지방+꾸지뽕군이 고지방군에 비하여 유의적으로 낮았다(p<0.05). 혈중 중성지방, HDL-콜레스테롤, 동맥경화지수는 고지방, 꾸지뽕 섭취에 따른 유의적인 차이가 없었다. 간의 총 콜레스테롤과 중성지방 함량은 고지방, 꾸지뽕 섭취에 따른 유의적인 차이가 없었다. 간 조직의 MDA 함량은 고지방군과 고지방+꾸지뽕군이 대조군에 비하여 유의적으로 높았다(p<0.05). 간 조직의 SOD 활성은 고지방+꾸지뽕군이 대조군과 고지방군에 비하여 유의적으로 높았으며(p<0.05), GSH-Px 활성, CAT 활성은 고지방, 꾸지뽕 섭취에 따른 유의적인 차이가 없었다. 결론적으로 고지방을 유도한 성장기 쥐에게 꾸지뽕잎 10%(100 g/kg dry diet) 첨가식이를 섭취시켰을 때 혈중 총 콜레스테롤과LDL-콜레스테롤은 유의적으로 낮았고, 간 조직의 SOD 활성은 유의적으로 높게 나타났다. 따라서 꾸지뽕잎은고지방을 섭취한 성장기 수컷 쥐에게 혈중 콜레스테롤 및 간 조직의 SOD 활성에 개선 효과를 보였다. The purpose of this study was to investigate the effects of Cudrania tricuspidata on serum lipids and antioxidant enzymes in growing rats fed a high-fat lard diet for six weeks. Twenty-four male Sprague-Dawley rats (body weight 50-60 g) were divided into three groups: control, high-fat diet (lard at 200 g/kg), and high-fat diet + Cudrania tricuspidata (lard 200 g/kg + Cudrania tricuspidata 100 g/kg). The results of the study indicate that mean intake was significantly lower for subjects in the high-fat diet group than for those in the control group and the high-fat + Cudrania tricuspidata group. Body weight gain was significantly lower in the high-fat diet group than in the control group and the high-fat diet + Cudrania tricuspidata group. The food efficiency ratio was significantly higher in the high-fat diet and high-fat + Cudrania tricuspidata groups than in the control group. The levels of serum total cholesterol and LDL cholesterol were significantly lower in the high-fat diet + Cudrania tricuspidata group than in the high-fat diet group. The levels of serum triglycerides and HDL cholesterol, and the atherogenic index (AI) did not significantly differ among the groups. The levels of liver total cholesterol and triglycerides did not significantly differ between the high-fat diet and the Cudrania tricuspidata-supplemented diet. The concentration of lipid peroxidation by measuring malondialdehyde (MDA) was significantly higher in high-fat diet group and in the Cudrania tricuspidata group than in the control group. Antioxidant enzymes in liver tissue, such as superoxide dismutase (SOD), were significantly higher in the high-fat diet + Cudrania tricuspidata group than in the control group and the high-fat diet group. Glutathione peroxidase (GSH-Px) and catalase (CAT) did not significantly differ between the lard-only diet and the Cudrania tricuspidata-supplemented diet. In conclusion, it is possible that Cudrania tricuspidata-supplemented diets may produce positive results on serum total cholesterol, LDL cholesterol, and antioxidant enzymes (such as SOD) in liver tissue.

고지방식이 운동후 글리코겐과 혈액성분 및 지구성 운동능력에 미치는 영향

정진원,강희석 한국운동영양학회 2000 Physical Activity and Nutrition (Phys Act Nutr) Vol.4 No.2

It is well known that high fat diet enhances using of fatty acids during the last stage of endurance exercise. But effects of high fat diet administration on endurance exercise were not well documented. At this point of view, we ingested intralipose and a high fat diet in rats to increase fatty acids utilization during endurance exercise performance. Thirty male Sprague-Dauley rats were chosen as the subjects in this experiment. These subjects were divided into two groups on the basis of diet protocols as follows : ① normal diet(carbohydrates 58%, proteins 23.8%, lipids 8%, and others), ② high fat diet(carbohydrates 45%, proteins 25%, lipids 30%). And in high fat diet group, intralipose was ingested before exercise. Endurance exercises on the basis of Bruce protocol were applied to the groups. Serum glucose, lactic acid, FFA, TG, cholesterol concentration were analyzed. Liver, soleus and gastrocnemius white muscle glycogen contents were determined and endurance exercise time was measured. As a result of experiment, high fat diet group was decreased less than normal diet group in serum glucose concentration and in liver, soleus and gastronemius white muscle glycogen contents. On the other hand, serum FFA and TG concentration were increased more high fat diet group than nomal diet group, and serum lactic acid concentration were increased less high fat diet group than normal diet group. And endurance exercise time was increased after a single high fat diet. In conclusion, even though a single high fat diet, glycogen-sparing effect in rats was occurred.

Moderate exercise training is more effective than resveratrol supplementation for ameliorating lipid metabolic complication in skeletal muscle of high fat diet-induced obese mice

( Jun Hyun Jeong ),( Hee Geun Park ),( Young Ran Lee ),( Wang Lok Lee ) 한국운동영양학회 2015 Physical Activity and Nutrition (Phys Act Nutr) Vol.19 No.2

Purpose The aim of this study was to compare the effectiveness of moderate exercise training or resveratrol supplementation with a low fat diet on lipid metabolism in the skeletal muscle of high fat diet-induced obese mice. [Methods] C57BL/6J mice (5 weeks old, n = 30) were fed a high fat diet (45% fat) for 8 weeks first to make them obese. Afterward, all the mice were fed a low fat diet during 8 weeks of intervention with moderate exercise training and resveratrol supplementation. Before the intervention, the mice were separated into 3 groups: low-fat diet control (HLC; n = 10), low fat diet with resveratrol (HLR; n = 10) or low fat diet with exercise (HLE n = 10). The exercise group (HLE) performed treadmill running for 30-60 min/day at 10-22 m/min, 0% grade, 5 times/week for 8 weeks, while the resveratrol group (HLR) received a daily dose of resveratrol (10 mg/kg of body weight), 5 days/week for 8 weeks. [Results] Body weight was significantly reduced in HLE. Further, the lipogenesis marker SREBP and the inflammatory cytokine TNF-α were significant reduced in HLE. However, there was no significant effect from resveratrol supplementation with a low fat diet. Taken together, exercise training with a low fat diet has the positive effect of ameliorating lipid disturbance in the skeletal muscle of high fat diet-induced obese mice. [Conclusion] These findings suggest that exercise training with a low fat diet is most effective to improve lipid metabolism by reducing lipogenesis and inflammation in the skeletal muscle of high fat diet-induced obese mice.

고지방식이 공급에 따른 성장기 마우스의 골의 형태학적 미세구조와 염증지표 변화

김미성(Kim Mi-Sung),이현아(Lee Hyun-A),김옥진(Kim Ok-Jin),손정민(Sohn Cheong-Min) 韓國營養學會 2011 Journal of Nutrition and Health Vol.44 No.6

본 연구에서는 성장기 마우스 모델을 이용하여 고지방식이를 제공한 후 이에 따른 염증지표와 골의 형태학적 미세구조의 변화를 살펴보았다. C57BL/6J 4주령 수컷 마우스를 난괴법에 의해 대조군 (n = 6)과 실험군 (n = 6)로 분류하여 대조군에는 10% Kcal fat 식이와 고지방식이군에는 45% Kcal fat 식이를 12주 동안 자유급식 방법으로 제공하였다. 혈액검사와 염증지표를 분석하였으며 micro-CT를 이용하여 대퇴부 뼈의 형태학적 미세구조를 측정하였다. 대조군과 고지방식이군의 체중 증가는 각각 5.85 ± 1.84 g, 16.06 ± 5.64 g로 유의한 차이를 보였으며 (p < 0.01), 혈당은 각각 115.00 ± 16.88 mg/dL, 188.33 ± 13.29 mg/dL (p < 0.01), 중성지방은 각각 65.00 ± 6.19 mg/dL, 103.33 ± 8.02 mg/dL (p < 0.05)로 나타났다. 렙틴과 IL-6는 고지방식이군에서 유의적으로 높게 나타났다 (p < 0.01). 골대사의 생화학적 지표 분석 결과 오스테오칼신은 고지방식이군에서 낮게 나타났으나 유의적이지 않았으며, CTx은 고지방식이군에서 유의적으로 높게 나타났다 (p < 0.01). 골밀도는 고지방식이군에서 낮게 나타났으나 유의적인 차이는 보이지 않았다. 그러나 골의 형태학적 미세구조 분석결과 골소주의 두께는 고지방식이군이 대조군보다 유의적으로 좁게 나타났으며 (p < 0.05), 골 소주의 간격은 고지방식이군이 유의적으로 넓게 나타났다 (p< 0.05). 골의 형태학적 미세구조인 골소주의 간격과 IL-6가 양의 상관성이 나타났다 (p < 0.05). 본 연구결과 최대골밀도가 형성되는 단계의 성장기 마우스에서 고지방식이 공급을 통한 비만 유도 현상은 골소주의 수와 골소주가 차지하는 비율의 변화를 유발하여 골 미세구조에 영향을 미치는 것으로 나타났으며, 염증지표와 상관성이 나타났다. 이에 성장기에 염증지표 증가를 억제하고 정상적인 골형성을 위하여 과잉의 지방섭취 제한이 필요할 것으로 사료된다. Obesity not only reduces bone mineral density but also increases inflammatory markers. Therefore, we examined the change in inflammatory markers and morphological microstructure of the bones using a mouse model fed a high-fat diet. C57BL/6J 4-week-old male mice were divided into a control group (n = 6) and a experimental group (n = 6); the control group was provided with 10% Kcal fat diet, and the high-fat diet group was provided with 45% Kcal fat diet for 12 weeks using the free provision method. Blood was analyzed for inflammatory markers, and micro-computed tomography was used to measure the morphological microstructure of the femoral bone. The weight increases in the control group and high-fat diet group were 5.85 ± 1.84 g and 16.06 ± 5.64 g, respectively (p < 0.01), glucose was 115.00 ± 16.88 mg/dL and 188.33 ± 13.29 mg/dL (p < 0.01), and triglycerides were 65.00 ± 6.19 mg/dL and 103.33 ± 8.02 mg/dL (p < 0.05) respectively. Leptin and interleukin (IL)-6 were significantly higher in the high-fat diet group than that in the control group (p < 0.01). As a result of a biochemical index analysis of bone metabolism, osteocalcin tended to be lower in the highfat diet group, whereas CTx was significantly higher in the high-fat diet group compared to that in the control group (p < 0.01). The thickness of the bony trabecula was significantly narrower in the high-fat diet group than that in the control group (p < 0.05), and the gap in the bony trabecula was significantly wider in the high-fat diet group than that in the control group (p < 0.05). IL-6 and the gap in the bone trabecula, which was a morphological microstructure of the bones, showed a positive correlation (p < 0.05). Taken together, inducing obesity through a high-fat diet in mice during the growth phase caused a change in bone microstructure and was correlated with the inflammation index. Accordingly, restriction of excessive fat intake may be needed to suppress the inflammatory reactions and promote normal bone formation. (Korean J Nutr 2011; 44(6): 481~487)

Anti-obesity effects of traditional and standardized <i>meju</i> in high-fat diet-induced obese C57BL/6J mice

Bae, Cho-Rong,Kwon, Dae-Young,Cha, Youn-Soo the Society for Free Radical Research Japan 2014 Journal of clinical biochemistry and nutrition Vol.54 No.1

<P>The aim of the study was to evaluate the anti-obesity effects of two types of <I>meju</I> in diet induced obese C57BL/6J mice. Animals were randomly divided into 4 dietary group (<I>n</I> = 10); normal diet, high fat diet with 30% soybean, high fat diet with 30% traditional <I>meju</I>, high fat diet with 30% standardized <I>meju</I>. After 16 weeks, after animals were sacrificed. It was observed that the high fat diet with 30% traditional <I>meju</I> and high fat diet with 30% standardized <I>meju</I> significantly reduced body weight gain, epididymal fat weight, serum triglyceride along with serum insulin and leptin levels compared to the high fat diet with 30% soybean. And also, the expression levels of hepatic lipid anabolic genes were significantly decreased in the high fat diet with 30% traditional <I>meju</I> and high fat diet with 30% standardized <I>meju</I> compared to the high fat diet with 30% soybean. In conclusion, the assessment of all the obesity markers strongly advocate the anti-obesity effect of traditional as well as standardized <I>meju</I> in diet induce obesity conditions.</P>

IL-10 Deficiency Aggravates Renal Inflammation, Fibrosis and Functional Failure in High-Fat Dieted Obese Mice

Kim Dae Hwan,Chun So Young,이은혜,Kim Bomi,Yoon BoHyun,Gil Haejung,Han Man-Hoon,Ha Yun-Sok,Lee Jun Nyung,Kwon Tae Gyun,김범수,장병익 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.3

BACKGROUND: High-fat diet-induced obesity is one of the major cause of chronic renal failure. This obesity-related renal failure is mainly caused by inflammatory processes. However, the role of the major anti-inflammatory cytokine interleukin (IL)-10 has not been researched intensively. METHODS: To evaluate the effect of IL-10 deficiency on obesity-related renal failure, the in vivo study was carried with four animal groups; (1) Low-fat dieted C57BL/6 mice, (2) Low-fat dieted IL-10 knockout (KO) mice, (3) High‐fat dieted C57BL/6 mice and (4) High‐fat dieted IL-10 KO mice group. The analysis was carried with blood/urine chemistry, H&E, Oil-Red-O, periodic acid-Schiff and Masson’s trichrome staining immunohistochemistry and real-time PCR methods. RESULTS: At week 12, high‐fat dieted IL-10 KO mice showed 1) severe lipid accumulation in kidneys, cholesterol elevation (in total, serum kidney) and low-density lipoprotein increasion through the SCAP-SREBP2-LDLr pathway; (2) serious histopathologic alterations showing glomerulosclerosis, tubulointerstitial fibrosis and immune cell infiltration; (3) increased pro‐inflammatory cytokines and chemokines expression; (4) enhanced renal fibrosis; and (5) serious functional failure with high serum creatinine and BUN and proteinuria excretion compared to other groups. CONCLUSION: IL-10 deficiency aggravates renal inflammation, fibrosis and functional failure in high-fat dieted obese mice, thus IL-10 therapy could be applied to obesity-related chronic renal failure. BACKGROUND: High-fat diet-induced obesity is one of the major cause of chronic renal failure. This obesity-related renal failure is mainly caused by inflammatory processes. However, the role of the major anti-inflammatory cytokine interleukin (IL)-10 has not been researched intensively. METHODS: To evaluate the effect of IL-10 deficiency on obesity-related renal failure, the in vivo study was carried with four animal groups; (1) Low-fat dieted C57BL/6 mice, (2) Low-fat dieted IL-10 knockout (KO) mice, (3) High‐fat dieted C57BL/6 mice and (4) High‐fat dieted IL-10 KO mice group. The analysis was carried with blood/urine chemistry, H&E, Oil-Red-O, periodic acid-Schiff and Masson’s trichrome staining immunohistochemistry and real-time PCR methods. RESULTS: At week 12, high‐fat dieted IL-10 KO mice showed 1) severe lipid accumulation in kidneys, cholesterol elevation (in total, serum kidney) and low-density lipoprotein increasion through the SCAP-SREBP2-LDLr pathway; (2) serious histopathologic alterations showing glomerulosclerosis, tubulointerstitial fibrosis and immune cell infiltration; (3) increased pro‐inflammatory cytokines and chemokines expression; (4) enhanced renal fibrosis; and (5) serious functional failure with high serum creatinine and BUN and proteinuria excretion compared to other groups. CONCLUSION: IL-10 deficiency aggravates renal inflammation, fibrosis and functional failure in high-fat dieted obese mice, thus IL-10 therapy could be applied to obesity-related chronic renal failure.

운지버섯 배양액이 고지방 식이를 섭취한 흰쥐의 단백질 및 무기질 농도에 미치는 영향

고진복 신라대학교 자연과학연구소 2004 自然科學論文集 Vol.13 No.-

운지버섯 균사체 배양액이 고지방 식이를 섭취한 성장기 흰쥐의 영양생리에 미치는 영향을 관찰하고자 생후 7주령의 숫쥐에 표준식이를 급여한 정상군, 고지방군 (15% 돈지첨가), 고지방식이와 음료수에 20% 및 30% 수준으로 운지버섯 균사체 배양액을 혼합한 군 (20% 및30% 운지버섯군)등 4군으로 나누어 5주간 사육하였다. 실험동물의 체중증가랑은 30% 운지버섯군이 고지방군에 비해 다소 감소되었다. 식이섭취량 및 식이효율, 그리고 간 및 신장의 무게는 고지방군과 20% 및 30% 운지버섯군이 비슷한 수준으로 나타났다. 부고환지방은 정상군에 비해 고지방군 및 운지버섯군들이 유의하게 증가되어 운지버섯 섭취에 따른 감소효과는 나타나지 않았다. 혈청의 칼슘, 무기인 및 마그네슘 농도는 고지방군에 비하여 20% 및 30% 운지버섯군이 유의하게 증가하여 정상군과 비슷한 농도로 나타났다. 혈청의 총 단백질, 알부민, 혈당 및 혈색소 농도는 정상군과 각 실험군들이 비슷한 수준으로 운지버섯 음료 섭취에 따른 영향은 나타나지 않았다. 혈청의 GOT, GPT, γ-GTP 및 ALP 활성은 정상군과 각 실험군이 비슷한 경향이었다. 혈청의 LDH 활성은 정상군과 20% 및 30% 운지버섯군은 비슷하였으나 고지방군은 유의하게 감소되었다. 이상의 결과로 보아 고지방식이를 급여한 흰쥐에 운지버섯 균사체 배양액을 20%나 30%섭취에 의한 체중증가량과 장기 무게, 단백질농도 및 효소 활성에는 영향을 받지 않는 것으로 나타났으나, 혈청의 칼슘, 무기인 및 마그네슘 농도를 증가시키는 것으로 나타났다. The effects of liquid culture of Coriolus versicolor on weight gain, food intakes, food efficiency ratios, serum protein and mineral levels, and serum enzyme activities, were studied in growing male rats. Sprague-Dawley rats were given four different types of diets for a succeeding period of 5 weeks, respectively: a normal diet group (7% corn oil), a high fat diet group (7% corn oil + 15% lard), a 20% or 30% C. versicolor diet groups (high fat diet + 20% or 30% C. versicolor in water) according to the levels of C. versicolor supplementation. The body weight gains and food intake of rats fed the 30% C. versicolor diets were lower than those in rats fed the high fat diet. The food efficiency ratios, liver and kidney weights of rats fed the 20% or 30% C. versicolor diets were similar to those of rats fed the high fat diet. But the epididymal fat pad weight of rats the fed high fat diet and the 20% or 30% C. versicolor diets were :significantly higher than that of rats fed the normal diet. The concentrations of the serum calcium, phosphorus and magnesium of rats fed the 20% and 30% C. versicolor diets were more significantly increased compared to rats fed the high fat diet, but those of rats fed the 20% and 30% C. versicolor diets were similar to those of rats fed the normal diet. The concentrations of total protein, albumin, hemoglobin and glucose in serum of rats fed the high fat diet, 20% and 30% C. versicolor diets were similar to those of rats fed the normal diet. There were no differences in the activities of glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, y -glutamyltranspeptidase and alkaline phosphatase in the serum among the experimental groups. The lactic dehydrogenase activity in serum of rats fed the 20% and 30% C. versicolor diets were similar to those of rats fed the normal diet, but that of rats fed the high fat diet was significantly decreased compared with that of rats fed the normal diet. These results showed that the 20% and 30% C. versicolor diets feeding increased the concentrations of serum calcium, phosphorus and magnesium in rats fed high fat diet.

Effect of moderate exercise training and low-fat diet on peritoneal macrophage immunocompetence in high-fat diet-induced obese mice model

정준현,박희근,이영란,권순미,장학영,전종귀,김명화,이상기,이광무,이왕록 한국운동영양학회 2012 Physical Activity and Nutrition (Phys Act Nutr) Vol.16 No.3

This study investigated the effect of moderate exercise training on macrophage immunocompetence in high fat diet-induced obese mice. To determine the differential effects of exercise training and low fat diet on macrophage, C57BL/6 male mice (4 week old,n = 32) were fed high-fat diet for 5 weeks to induce obese. The high fat-induced obese mice were then divided into four groups and differently treated by high/low fat diet and exercise training for a further 8 weeks; HHC (13 weeks high-fat diet), HHE (13weeks high-fat diet with 8 weeks Exercise training) and HLC (5 weeks high-fat diet and 8 weeks low-fat diet), HLE (5 weeks high-fat diet and 8 weeks low-fat diet with exercise training). The exercise groups were performed on a motorized treadmill, running for 30-60 min/day at 10-22 m/min, 0% grade for 8wks. Body weight was significantly decreased for those with a low-fat diet and exercise training (HLE). Macrophage phagocytosis was significantly increased by low-fat diet and exercise training (HLE). The percentage of stimulation of NO release induced by LPS was higher in HLE than in other groups. The percentage of stimulation of IL-1βrelease induced by LPS was higher in HHE and HLE than in other groups. The percentage of stimulation of IFN-γ release induced by LPS was higher in HLE than in other groups. These findings suggest that moderate exercise and low-fat diet have beneficial effects on macrophage immunocompetence in high fat diet-induced obese mice.