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PCR 기법을 이용한 사탕무씨스트선충과 콩씨스트선충의 간이동정
고형래,김은화,김세종,이재국,이왕휴,Ko, Hyoung Rai,Kim, Eun Hwa,Kim, Se Jong,Lee, Jae Kook,Lee, Wang Hyu 한국식물병리학회 2017 식물병연구 Vol.23 No.3
강원도 고랭지배추 포장에서 검출된 사탕무씨스트선충(H. schachtii)과 콩씨스트선충(H. glycines)을 구별할 수 있는 신속진단법을 개발하고자 하였다. 이를 위해 mtDNA COI 유전자 영역의 계통분석으로 동정된 사탕무씨스트선충 GC147, GC408, PM001 개체군과 콩씨스트선충 YS224, DA142, BC115 개체군을 대상으로 PCR-RFLP와 본 연구에서 개발한 특이 프라이머 세트를 이용한 PCR을 수행하였다. 사탕무씨스트선충과 콩씨스트선충 각각 3개 개체군의 mtDNA COI 영역 PCR 증폭산물에 8종류의 제한효소를 처리하여 DNA 절편길이다형성을 확인하였으며, 2종류의 제한효소 RsaI과 HinfI을 처리하면 DNA 밴드 양상의 차이로 사탕무씨스트선충과 콩씨스트선충 두 종을 구별할 수 있었다. 또한, 본 연구에서 개발한 프라이머 세트(JBS1, JBG1과 JB3R)는 사탕무씨스트선충 mtDNA COI 영역의 277과 339 bp, 콩씨스트선충의 339 bp의 특정 DNA 단편을 증폭시켰으며, 뿌리혹선충 3종과 뿌리썩이선충 2종의 식물기생선충은 증폭시키지 않았다. 따라서, 본 연구에서 개발한 프라이머 세트를 이용하여 PCR을 수행하면 사탕무씨스트선충과 콩씨스트선충을 구별할 수 있었다. The purpose of this study was to develop rapid methods for distinguishing between Heterodera schachtii and H. glycines detected from chinese cabbage fields of highland in Gangwon, Korea. To do this, we performed PCR-RFLP and PCR with the primers set developed in this study for GC147, GC408 and PM001 population, H. schachtii, and YS224, DA142 and BC115 population, H. glycines. Eight restriction enzymes generated RFLP profiles of mtDNA COI region for populations of H. schachtii and H. glycines, repectively. As a result, treatment of two restriction enzymes, RsaI and HinfI, were allowed to distinguish H. schachtii from H. glycines based on the differences of DNA band patterns. The primer set, #JBS1, #JBG1 and #JB3R, amplified specific fragments with 277 and 339 bp of H. schachtii, 339 bp of H. glycines, respectively, while it did not amplify fragments from three root-knot nematodes and two root-lesion nematodes. Thus, the primer set developed in this study could be a good method, which is used to distinguish between H. schachtii and H. glycines.
콩씨스트선충(Heterodera glycines)의 밀도가 콩 수량에 미치는 영향
강헌일(Heonil Kang),박병용(Byeongyong Park),박세근(Sekeun Park),김은화(Eunwha Kim),박은형(Eunhyeong Park),고형래(Hyoung-Rai Ko) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.4
본 연구는 토양 내 콩씨스트선충(Heterodera glycines)의 초기 밀도에 따른 콩의 수량구성요소 및 수량에 미치는 영향을 규명하기 위하여 실시하였다. 재배포를 35개의 구역을 나누고 콩씨스트선충의 파종 전 토양 내 씨스트 밀도와 알 밀도를 조사하였으며, 수확기 콩의 초장, 협수, 립수, 백립중을 비교 분석하였다. 초기 씨스트의 밀도보다 씨스트 내 알의 밀도가 수량에 더욱큰 영향을 미쳤으며, 초기 알 밀도가 높을수록협수와 수량이 초기 알 밀도가 높을수록 감소하는 경향을 보였고 토양 100 ㎤ 당알의 밀도가 2,000개 이상일 때부터 수량이 20% 이상 급격하게감소하였다. 본 연구결과를 통하여 콩 재배 시 콩씨스트선충에의한 피해를 예측할 수 있으며, 국내 콩 재배지의 약 40%가 감염되어 있는 콩씨스트선충에 대한 저항성 품종 육성을 포함한 다양한 방제방법 연구가 필요하다. This study was conducted to investigate the yield loss of soybean by initial density of Heterodera glycines in soil. A soybean cultivar named ‘Cheongja5ho’ was cultivated as test material in Dangjin city, Chungcheongnam-do, Korea. Each seed was planted with distance of 25 cm in 35 plots by initial density of egg of the cyst nematode on June 21st, and allowed to harvest on November 12st in 2019. As a results, the yield of soybean decreased sharply when as the initial density of eggs of cyst nematode increased. The initial density of eggs in soil affected largely on the decreased yields of the soybean by more than 20% in above 2,000 eggs, and 30% in above 6,000 eggs of the cyst. Beyond the 6,000 eggs, the yield loss was more slowly decreased compared with the above cases. Eggs of H. glycines are widely distributed in Korea, resulting in the serious soybean yield loss by H. glycines.
Kim, Myung-Sik,Sung, Mi-Kyung,Kim, Min-Whan,Seo, Hyung-Jin,Kim, Dong-Geun,Chung, Jong-Il The Korean Society of Crop Science 2013 한국작물학회지 Vol.58 No.2
Soybean cyst nematode (Heterodera glycines Ichinohe) is one of the serious soybean [Glycine max (L.) Merr.] pests in major soybean producing countries. The objective of this study was to investigate of Heterodera glycines type using the five SCN infested soybean field soils and was to evaluate resistance to the soybean cyst nematode HG 2.5.7 type on soybean varieties and germplasms. The five SCN contaminated soil samples were collected from the three provinces on November 2011 in Korea, and eggs were cultured on early spring season in 2012. For the second study, a total fifty nine soybean varieties and germplasms were tested by infestation of HG type 2.5.7 in the greenhouse. Soybean cyst nematode HG types were investigated from five locations, HG 2 (race 1) type at Donghae, HG 2.5 (race 1) type at Jeongseon and Hapcheon, HG type 2.5.7 (race 1 or 5) at Yeongwol, and HG 1.2.7 (race 5) type at Haenam locations in present study. No Korean soybean varieties and germplasms were observed with SCN resistant trait to the HG type 2.5.7. Average SCN female index were calculated with 82.7% in 59 plant materials. Our results could be provided useful information to develop a SCN resistant cultivar in Korea.
김명식,정종일,성미경,김민환,서형진,김동근 한국작물학회 2013 Korean journal of crop science Vol.58 No.2
Soybean cyst nematode (Heterodera glycines Ichinohe) is one of the serious soybean [Glycine max (L.)Merr.] pests in major soybean producing countries. The objective of this study was to investigate of Heterodera glycines type using the five SCN infested soybean field soils and was to evaluate resistance to the soybean cyst nematode HG 2.5.7type on soybean varieties and germplasms. The five SCN contaminated soil samples were collected from the three provinces on November 2011 in Korea, and eggs were cultured on early spring season in 2012. For the second study, a total fifty nine soybean varieties and germplasms were tested by infestation of HG type 2.5.7 in the greenhouse. Soybean cyst nematode HG types were investigated from five locations, HG 2 (race 1) type at Donghae, HG 2.5 (race 1) type at Jeongseon and Hapcheon, HG type 2.5.7 (race 1 or 5) at Yeongwol, and HG 1.2.7 (race 5) type at Haenam locations in present study. No Korean soybean varieties and germplasms were observed with SCN resistant trait to the HG type 2.5.7. Average SCN female index were calculated with 82.7% in 59plant materials. Our results could be provided useful information to develop a SCN resistant cultivar in Korea
Myung-Sik Kim,Mi-Kyung Sung,Min-Whan Kim,Hyung-Jin Seo,Dong-Geun Kim,Jong-Il Chung 韓國作物學會 2013 Korean journal of crop science Vol.58 No.2
Soybean cyst nematode (Heterodera glycines Ichinohe) is one of the serious soybean [Glycine max (L.) Merr.] pests in major soybean producing countries. The objective of this study was to investigate of Heterodera glycines type using the five SCN infested soybean field soils and was to evaluate resistance to the soybean cyst nematode HG 2.5.7 type on soybean varieties and germplasms. The five SCN contaminated soil samples were collected from the three provinces on November 2011 in Korea, and eggs were cultured on early spring season in 2012. For the second study, a total fifty nine soybean varieties and germplasms were tested by infestation of HG type 2.5.7 in the greenhouse. Soybean cyst nematode HG types were investigated from five locations, HG 2 (race 1) type at Donghae, HG 2.5 (race 1) type at Jeongseon and Hapcheon, HG type 2.5.7 (race 1 or 5) at Yeongwol, and HG 1.2.7 (race 5) type at Haenam locations in present study. No Korean soybean varieties and germplasms were observed with SCN resistant trait to the HG type 2.5.7. Average SCN female index were calculated with 82.7% in 59 plant materials. Our results could be provided useful information to develop a SCN resistant cultivar in Korea.