일부 폐결핵환자의 HBs Ag. 발현빈도와 HBs Ag. 음양성자 간의 GOT 치와 GPT 치의 비교 검토

김태전,김승곤 서울保健大學 1981 論文集 Vol.1 No.1

폐결핵환자 300명을 대상으로 HBs ag.와 GOT와 GPT치를 검사한 성적을 요약하면 다음과 같다. 1) 폐결핵환자에서의 HBs ag. 양성율은 20%로서 대조군의 4.1%보다 유의하게 높았다(p<0.05). 2)HBs ag. 양성자의 GOT(43.2±2.0unit), GPT(29.3±1.8unit)치는 음성자의 GOT(35.4±1.3unit), GPT(19.3±1.9unit)치보다 다소 높은 경향을 보였다. 따라서 폐결핵환자 치료시에는 완전 멸균된 주사침을 사용해야 함은 물론 항결핵제가 간에 미치는 부작용을 감안 HBs ag.에 대한 검사를 반드시 실시해야 할 것이며 HBs ag.의 음양성이 GOT치나 GPT치에 큰 영양을 주지는 못하는 것으로 생각된다. This study was done to see HBs antigen positive rate of patients, with pulmonary tuberculosis, and to compare GOT, GPT levels between HBs antigen positive and negative patients. The results obtained were summarized as following: 1) As HBs antigen positive rate was 20% in patients with pulmonary tuberculosis, it was significantly higher than HBs positive rate (4.1%) of control group. (P<0.05). 2) GOT, GPT levels of HBs antigen positive patients (GOT:41.3±2.0 unit, GPT:29.3±1.8 unit) had a slightly higher trend than those of HBs antigen negative ones. As the results of this study, when patients were medicated for pulmonary tuberculosis, needles completely sterilized should be used, also tests of HBs antigen had to be done in them, and it was thought that GOT, GPT levels were not largely influenced by occurrance of HBs antigen.

B형 간염 바이러스 감염과 연관된 사구체신염의 임상병리학적 특성과 신 조직내에서 HBs Ag 과 HBe Ag의 발현

정수진,김영주,윤혜경,정우영,김영훈,김수영,Jung Soo-Jin,Kim Young-Joo,Yoon Hye-Kyoung,Chung Woo-Yeong,Kim Young-Hoon,Kim Su-Yung 대한소아신장학회 1998 Childhood kidney diseases Vol.2 No.1

목적 : HBV 감염과 연관된 신병증의임상 및 검사소견과 병리학적 소견을 분석하여 그 특징을 규명하고, HBeAg과 HBsAg에 대한 면역조직화학염색을 신생검조직에서 시행하여 이들 항원의 사구체내에서의 면역학적 발현을 관찰하므로써 이 질환의 발생 기전에 대한 이해를 돕고자하였다. 방법 : 1990년 4월부터 1997년 2월까지 사구체신염으로 신조직 생검을 시행하여 부산백병원 해부병리과에 병리조직검사가 의뢰된 증례 중 혈청 HBsAg 양성인 28례를 대상으로 임상 및 검사소견을 조사하였고, 신조직의 광학현미경적, 면역형광학적, 전자현미경적 검사와 HBsAg과 HBeAg 항원에 대한 면역조직화학검사를 시행하였다. 결과 : 1. 연령 분포는 6세에서 73세였으며 소아 환자가 8명이었고 성인 환자는 20명 이었다. 평균 연령은 28세였고, 남녀 비는 3:1 이었다. 내원당시 67.9%에서 혈뇨, 75.0%에서 단백뇨를 보였고 57.2%에서 신증후군을 나타내었다. 전 예에서 혈청 HBsAg이 양성이었고, 혈청 HBeAg은 검사가 시행된 9례 중 6례 (66.6%)에서 양성이었다. 2. 간기능검사가 시행된 11례 중 7례 (63.6%)에서 AST와 ALT치가 증가되어 있었으며, 이중 간생검이 시행된 3례 모두 만성 활동성 간염으로 진단되었다. 3. 28례 중 막성 사구체 신염 (membranous glomerulonephritis: MGN)이 10례 (35.7%)로 가장 많았으며, 메산지움중식성 사구체신염 (mesangioproliferative glomerulonephritis: MesGN)이 8례 (28.6%), 막증식성 사구체 신염 (membranoproliferaive glomerulonephritis : MPGN)은 7례 (25.0%) 그리고 미세변화질환 (minimal change disease : MCD)가 3nl (10.7%)순이었다. 4. HBV와 연관된 MGN에서는 혈관간 세포의 증식 및 혈관간 기질의 확장이 자주 관찰되었으며, 사구체간질 내와 내피하 침윤도 동반되어 MPGN을 시사하는 소견이 함께 관찰되는 예가 많았고, HBV 항원 혈증에 동반된 MPGN에서는 내피하 침윤 뿐 아니라 기저막 내 및 상피세포하 침윤도 종종 관찰되었다. 5. 신조직의 면역조직화학검사상 HBsAg은 모두 음성이었으나 HBeAg은 28례 중 8례 (28.6%)에서 사구체 모세혈관벽을 따라 양성 반응을 나타내었고, 이 중 3례는 혈치 HBeAg 검사를 받았는데 모두 양성이었다. 결론 : HBV와 연관된 사구체신염은 다양한 병리학적 양상을 보여주며, 특히 MGN과 MPGN에서는 전자현미경소견상 두 질환의 특징이 중복되어 나타났고, 사구체내에서 HBeAg이 검출되어 HBV의 활동성 정도와 사구체신염의 발생이 연관성이 있을 것으로 사료되나 더 많은 연구보고가 있어야 하겠다. Purpose : Hepatitis B virus (HBV) infection has been involved in several forms of immune-related glomerulopathy but the pathogenic role of HBV infection is not clear. To evaluate the clinicopathological features of HBV-associated glomerulopathy, a clinicopathological analysis and immunohistochemical stain for HBs Ag and HBe Ag were done. Methods : Clinicopathological features of HBV-associated glomerulopathy were analyzed with renal biopsies in 28 HBsAg seropositive patients from April 1990 to February 1997 at Pusan Paik Hospital, and immunohistochemical evaluation for HBsAg and HBeAg was done in renal tissues. Light microscopic, immunofluorescent and electron microscopic examination and immunohistochemical staining for HBsAg (DAKO) and HBeAg (BIONIKE) of renal tissue were performed. Result ; 1. The age distribution was 6 to 73 years old, and eight were children and 20 were adults. Male : female ratio was 3:1. Nineteen (67.9%) and 21 (75.0%) of 28 cases showed hematuria and proteinuria, respectively at the time of biopsy. Sixteen (57.2%) of them had nephrotic syndrome. 2. Liver function test was performed in 11 patients and seven (63.6%) of them showed increased AST and ALT levels. Liver biopsy was done in three patients and revealed findings of chronic active hepatitis. 3. HBV-associated glomerulopathy was membranous glomerulonephritis (MGN) in 10 (35.7%), mesangiopathy in 8 (28.6%), membranoproliferative glomerulonephritis (MPGN) in 7 (25.0%) and minimal change disease in 3 (10.7%) out of 28 cases. 4. Ultrastructurally HBV-associated MGN showed conspicuous subepithelial deposits with intramembranous, mesangial and subendothelial deposits and proliferation of mesangial cells and matrix, which were suggestive of MPGN. In HBV-associated MPGN, intramembranous and subepithelial deposits were scattered. 5. Immunohistochemical staining revealed no expression for HBsAg, but positive reaction for HBeAg along capillary wall in 8 cases (28.6%), of which 3 cases were checked for serum HBeAg, all showed positivity. Conclusion : HBV-associated glomerulopathy showed a wide morphologic spectrum and overlapping ultrastructural features in MGN and MPGN, and the activity of hepatitis B virus may be related to the development of HBV-associated glomerulopathy but further studies are recommended to confirm this relationship.

일부 헌혈자들에 대한 실태조사

김태전 대한임상병리사협회 1979 대한임상병리사회지 Vol.11 No.1

This study was made on the sexes, ages, resident areas, occupations, educational levels. the number of blood donation, and HBs antigen associated with serum hepatitis from 852volunteer blood donors inorder to see their social ladder, voluntary spirit, and HBs ag. positive rete. The results were summarlized as followings: 1) Male, 16-20 ages group, Seoul area, students, middle and high school graduates, and the first donors of them had trend the highest. 2) In them HBs ago positive rate was 4.1%. In the vast social ladder, therefore, more people should take in blood donation. voluntarily. HBs ag. positive rate was reduced more than in the past but still higher than in the advanced nations, and it is necessary to develop immunological prevention against serum hepatitis.

HBV : Prediction of HBV DNA Negativity 6 Months after the Antiviral Therapy in both HBeAg Positive and Negative Hepatitis Using Quantitative Serum HBsAg Level; A Pilot Study

( Young Hoon Kim ),( Jung Il Lee ),( Ja Kyung Kim ),( Kwan Sik Lee ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background: Although the ultimate goal of antiviral therapy might be the eradication of HBs Ag, due to the rarity of this event, maintaining the HBV DNA negativity might be the realistic goal in both HBe Ag (+) and (-) hepatitis with nucleos(t) ide analogue treatments. Several reports demonstrated that ability of HBs Ag levels to predict HBe Ag seroconversion after the antiviral therapy in HBe Ag (+) hepatitis. This study sought to investigate the possible usefulness of quantitative serum HBs Ag level in predicting HBV DNA negativity after the oral nucleos(t)ide administration in both HBe Ag (+) and (-) hepatitis with or without liver cirrhosis. Methods: Patients who underwent antiviral therapy and had their serum HBs Ag checked quantitatively before the treatment and at least 3 consecutive times after the treatment were recruited for this retrospective study. Results: From 180 chronic HBV patients who were put under oral nucleos(t)ide analogues and had their serum HBs Ag checked quantitatively, 103 patients had consecutive HBs Ag levels checked before the antiviral treatment and at least 3 times consecutively after the initiation of the treatment at 3 months intervals (M: F=62:41). From 34 patients with HBe Ag (+) hepatitis, 11 patients (16.4%) achieved HBV DNA negativity by 6 months after the treatment whereas among 64 HBe Ag (-) patients, 56 (83.6%) reached HBV DNA negativity. Decline of serum HBs Ag level differed significantly between the patients that reached HBV DNA negativity and that who failed to do so (P=0.013). The Factors that could predict the HBV DNA negativity after the 6 months of oral nucles(t)de analogues were HBe Ag positivity (CI 0.052-0.729, HR 0.194, P=0.002), accompanying liver cirrhosis (CI 0.065-0.944, HR 0.248, P=0.041), and Log (HBV DNA/HBsAg) 1 month after the initiation of the treatment (CI 0.243-0.731, HR0.421, P=0.021). Conclusions: Quantitative HBs Ag level 1 month after the initiation of the oral nucleos(t)ide analogue administration, together with HBV DNA level, might be useful in predicting HBV DNA negativity 6 months after the therapy.

강원도 일부 지역주민들에서의 간염 B항원 발현율에 관한 조사

김태전,문희주 최신의학사 1979 最新醫學 Vol.22 No.12

This study was done to report HBs antigen positive rate to a part of inhabitants at Kang Woon Do, and compare between the urban and the rural the occurrence rate of HBs antigen. The following results were obstained in this study ; 1)In the rural, HBs antigen positive rate was 7.3%, and it was not significantly higher than urban. 2)In sex and ages; HBs antigen positive rate generally indicated higher trend in male than female, in younger than older people. 3)Especially, preschool children was statistically the highest than student group and adult group in HBs antigen positive rate (P<0.01). 4)The relationship of blood type and HBs antigen positive rate was not significant statistically. In the above results, there could see that the infection of hepatitis B virus had a very close relationship to unsanitary environment, low economic and cultural level. Especially there should consider a healthy countermeasure for preschool and primary school children in rural area.

B형 간염 바이러스 양성인 만성 간질환에서 Hepatitis B표면항원의 아형

김정숙,이채훈,임소여,조희순,김경동 영남대학교 의과대학 1996 Yeungnam University Journal of Medicine Vol.13 No.2

B형 간염 표면항원(HBsAg)의 4가지 주요 아형 조사를 통해 HBsAg과 B형 간염 표면항체가 모두 양성인 환자에서 항원과 항체의 아형이 다름을 밝힘으로 항원-항체 공존을 이해하게 되었으며, 감염경로를 추적하는 역학적조사, 인구이동양상의 지표에 유용하게 사용될 수 있다. 현재까지 우리나라의 HBV아형에 관한 연구는 전체적인 빈도의 조사나 HBsAg과 anti-HBs가 공존하는 경우에서 아형이 가지는 의미에 대한것이 전부였다. 본 연구에서는 영남대학교 의과대학 부속병원에서 만성 간질환으로 진단받고 경과추적중인 HBsAg양성인 환자 214명을 대상으로 혈청에 존재하는 HBsAg아형을 단세포군 항체를 이용한 혈청학적 방법으로 조사하였다. 214명의 검체중 206명(93.9%)에서 adr,6명(2.8%)에서 adw, 2명(0.9%)에서 ayr, 2명(.09%)에서 ar, 3명(1.4%) adwr로 나타났으며 ayw는 한 례에서도 발견되지 않았다. 아형과 질환의 중증도 사이에는 유의한 연관성은 발견되지 않았다. 결론적으로 만성 간질환환자에서 adr이 기장 많고 질환의 중증도와 아형간에 유의한 연관성은 발견되지 않았지만, 아형이 만성 B형바이러스간염의 간경변증이나 간세포암으로의 진행과 예후에 영향을 미치는지 밝히기위해서는 좀 더 연구를 진행하여야 할 것이다. Four subtypes of hepatitis B surface antigen are useful in the epidemiologic studies of the route of virus transmission and clinical significance of simultaneous occurance of hepatitis B surface antigen and antibody to hepatitis B surface antigen in the same serum as well as useful marker for population migration. The sera were obtained from 214 HBs Ag positive patients who are diagnosed as chronic liver disease and following up in the Yeungnam university hospital. The subtypes were determined by solid-phase sandwich EIA using monoclonal antibodies. Among 214 specimens, the subtype adr was 93.9%, adw was 2.8%, ayr was 0.9%, ar was 0.9%, adwr was 1.4% and ayw was not detected. There were no correlation between subtype pattern and disease. In summary, the subtype adr was prominent in our study and the difference of subtype pattern by severity of disease was not significant. However, to determine the prognostic value of HBs Ag subtype and relationship between subtype and disease progression, long-term follow up will be needed.

간세포암종에서의 B형 간염 표면항원(HBs Ag), 형질 전환 성장인자-알파 (TGF-α) 및 증식세포핵 항원(PCNA)의 발현에 관한 면역조직화학적 검색

허방,허만하 대한병리학회 1995 Journal of Pathology and Translational Medicine Vol.29 No.4

IMx 분석기를 이용한 HBs Ag, HBs Ab의 정도관리

권영일 ( Young Ii Kwon ) 대한임상검사과학회 1994 대한임상검사과학회지(KJCLS) Vol.26 No.1

Association of hepatitis B and C virus infection in psoriasis

( Hyun Yi Suh ),( Hong Lim Kim ),( Kyung Ho Kim ),( Jae Wook Jeon ),( Ji Young Ahn ),( Mi Youn Park ),( Yong Bum Yoon ),( Jai Il Youn ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Recent finding in psoriasis research have shown that psoriasis is not just a skin disease but frequently associated with systemic comorbidities. Viral hepatitis is known to be associated with chronicinflammation and aberrant immune response. Objectives: This study is to investigate prevalence and association of HBV and HCV infection in psoriasis. Methods: We analyzed and evaluated laboratory in patients with or without psoriasis who visit department of dermatology, National Medical Center from September 2012 to March 2015. 706 patients with psoriasis and 345 patients without psoriasis were enrolled. 240 patients with psoriasis and 345 patients without psoriasis were evaluatedHBV and HCV serological makers. Results: 4(1.7%) out of 240 patients with psoriasis were Hbs Ag positive while 7(2.07%) out of 345 patients without psoriasis in the control group were Hbs Ag positive. There was no significant difference between two groups. There was no significant associated with hepatitis B virus infection after adjusting for gender, age, and severity of psoriasis. 1out of 240 patients with psoriasis was anti-HCV positive and 1 out of 345 patients in the control group were anti-HCV positive. Conclusion: Psoriasis does not appear to be associated with an increased risk of hepatitis in our study. Epidemiology of viral infections in psoriasis needs to be continually studied and updated given importance in management considerations.

Clinical Implication of HBs Ag Quantification in Chronic Hepatitis B Patients Treated with TDF or ETV

( Jung Hyun Lim ),( Jung Hwan Yu ),( Young Ju Suh ),( Jin-woo Lee ),( Young-joo Jin ) 대한간학회 2021 춘·추계 학술대회 (KASL) Vol.2021 No.1