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        한국 재래종 무 및 갯무의 RAPD 분석에 의한 유연관계

        최우진(Wo-Jeen Choi),이선애(Sun-Ae Lee),유수미(Su Mi Yoo),이수성(Soo-Seong Lee),강정훈(Jung-Hoon Kang),고호철(Ho-Cheol Ko) 한국원예학회 2008 원예과학기술지 Vol.26 No.4

        Morphological characteristics of 41 entries consisting of 23 local cultivars and 18 wild collections (Gaenmoo) in radish (Raphanus sativus), were observed on the field and the genetic relationships were investigated with the random amplified polymorphic DNA (RAPD) technique. The root of 12 local cultivars designated ‘Jinju-daepyoung’ and the 5 others except ‘Altari-moo’ was as big as more than 1.3 ㎏ and 72 to 82% in portion of the plant weight. In the case of 6 ‘Altari-moo’, the root was as small as 0.6 ㎏ and 75% in portion of the plant weight. The wild radishes named ‘Gaenmoo’ are very similar in the morphology, even though they were collected from quite different areas. Their roots were as small as around 0.2 ㎏ and less than 50% in portion of the plant weight. Twenty two primers presenting more than 8 polymorphic bands in the test of a local cultivar of the ‘Jinju-daepyoung’ group and a wild accession of the ‘Gaenmoo’ group were selected among 146 operon random primers prior to genetic relationship investigation. They were applied to 41 entries and a dendrogram was figured with the results obtained. Twenty three local cultivars were divided into 3 different groups, the ‘Jinju-daepyoung’ group of 12 entries with the big root, another group of 5 entries with the big root and the ‘Aaltari-moo’ group of 6 small rooted entries. The 18 wild ‘Gaenmoo’ were also divided into 3 groups according to the collected area of Jeju Island, such as southern group of 5 entries, northern group of 6 entries including 3 south-east coast entries and the one-generation-selfed group of 4 entries originating from 2 plants of 2 collections in the southern part of Jeju Island. The small rooted local cultivar ‘Aaltari-moo’ was much more similar to the wild ‘Ggaetmoo’ radish rather than the big rooted local cultivar in the genetic relationship. In conclusion, 41 entries were divided into 2 main groups according to the plant weight and the size of the root.

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