Differences in liver microRNA profiling in pigs with low and high feed efficiency

( Yuanxin Miao ),( Chuanke Fu ),( Mingxing Liao ),( Fang Fang ) 한국축산학회 2022 한국축산학회지 Vol.64 No.2

Feed cost is the main factor affecting the economic benefits of pig industry. Improving the feed efficiency (FE) can reduce the feed cost and improve the economic benefits of pig breeding enterprises. Liver is a complex metabolic organ which affects the distribution of nutrients and regulates the efficiency of energy conversion from nutrients to muscle or fat, thereby affecting feed efficiency. MicroRNAs (miRNAs) are small non-coding RNAs that can regulate feed efficiency through the modulation of gene expression at the post-transcriptional level. In this study, we analyzed miRNA profiling of liver tissues in High-FE and Low-FE pigs for the purpose of identifying key miRNAs related to feed efficiency. A total 212~221 annotated porcine miRNAs and 136~281 novel miRNAs were identified in the pig liver. Among them, 188 annotated miRNAs were co-expressed in High-FE and Low-FE pigs. The 14 miRNAs were significantly differentially expressed (DE) in the livers of high-FE pigs and low-FE pigs, of which 5 were downregulated and 9 were upregulated. Kyoto Encyclopedia of Genes and Genomes analysis of liver DE miRNAs in high-FE pigs and low-FE pigs indicated that the target genes of DE miRNAs were significantly enriched in insulin signaling pathway, Gonadotropin-releasing hormone signaling pathway, and mammalian target of rapamycin signaling pathway. To verify the reliability of sequencing results, 5 DE miRNAs were randomly selected for quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR results of miRNAs were confirmed to be consistent with sequencing data. DE miRNA data indicated that liver-specific miRNAs synergistically acted with mRNAs to improve feed efficiency. The liver miRNAs expression analysis revealed the metabolic pathways by which the liver miRNAs regulate pig feed efficiency.

글루콘산아연, 프럭토올리고당 및 생균제의 급여가 부로일러의 육성성적에 미치는 영향

고용균,유선균,길용현,신종서,오진석 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2005 동물자원연구 Vol.16 No.-

This study was carried out to compare the effect of dietary zinc gluconate, fructooligosaccharide and probiotic on body weight gain, feed intake and feed efficiency of broiler chicks. A feeding trial was conducted with a total of 216 broiler chicks. Each treatment had 18 chicks with 3 replications. The supplementation levels of each treatment in the experimental diets were 0.20% for zinc gluconate, fructooligosaccharide and probiotic, respectively. The body gain of chicks fed feed additives significantly increased (p<.05) compare with that of control. Especially, the treatment with probiotic produced higher body gain than other treatments. Average feed intake of chicks was not significantly different among treatments (p<.05). Feed efficiency of chick fed feed additives significantly (p<.05) improved compare with control and the highest in the probiotic treatment. Therefore, the feed efficiency of chicks fed fructooligosaccharide, zinc gluconate and probiotic treatment were increased by 3.5%, 4.0% and 4.5%, respectively compare to that of the control. In conclusion, dietary feed additives tended to slightly improve the body gain and feed efficiency. Especially the treatment with probiotic produced higher body gain and feed efficiency among other treatments.

먹이조건의 차이가 지렁이 생육, 분립생산량 및 체조직으로의 유기물 전환효율에 미치는 영향

이지영,이주삼 한국유기농업학회 2008 韓國有機農業學會誌 Vol.16 No.3

This experiment was carried out to investigate the effect of different feed conditions on growth, cast production and conversion efficiency of organic matter to the earthworm. The experiment was tested on cow manure(CM) mixed with rice hulls (RH) or rice straw(RS). The mixture ratios were designed as one time, two times and three times of cow manure volume, respectively. The CM and RH mixtures resulted better on growth rate, reproductive efficiency and wormcast production than those for CM and RS mixtures. Especially on three times of RH mixture showed the highest growth characteristics compared to the other mixtures. Although both were between 20 and 34 on carbon and nitrogen ratio, the result inferred that the difference on the growth might have been caused by feeding conditions. On the mixtures of cow manure with rice hulls(CM+RH) was significantly higher on values on the conversion rate and conversion efficiency of organic matter to earthworm tissues than the mixtures of cow manure with rice straw(CM+RS). The most concerned point of the mixture of earthworm feed is that high contents of volatile solid and total carbon that increases in conversion efficiency of organic matter to earthworm tissues, and also causes the increase in growth rate and reproductive efficiency. The wormcast could be used as a valuable plant growth medium or soil conditioner for sustainable agriculture and it may be due to their high qualities of physico-chemical properties.

사료의 희석 및 무급여일 설정방법에 의한 조기제한사양이 육계의 보상성장과 사료효율 및 복강지방 축적에 미치는 영향

이규호,오용석,함영훈 한국가금학회 2002 韓國家禽學會誌 Vol.29 No.1

본 시험은 왕겨에 의한 사료의 희석과 무급여일 설정방법에 의한 조기제한사양이 육계의 보상성장과 사료효율 및 체지방 축적에 미치는 영향을 구명하기 위해 7일령된 무감별 육계 300수를 5처리 3반복, 반복당 20수씩 공시하여 7일령에서부터 49일령까지 실시하였다. 대조구(C)는 7일령부터 49일령까지 전기간 육계 전기사료와 후기사료를 자유채식을 시켰으며, 시험구(71-74)들은 모두 7일령부터 14일령까지 육계전기사료와 왕겨를 50:50(wt/wt)으로 혼합한 희석사료를 급여하였으며, 제한사양기간동안 T1은 희석사료를 자유채식시켰으며, T2는 1일절식-3일급여, T3는 1일절식∼2일급여 및 T4는 1일절식∼1일급여하였고, 제한 사양이 끝난 시험구들은 전기사료 및 후기사료를 자유채식시켰다. 제한사양기간을 포함한 21일령까지의 증체량과 21일령 체중 모두 T1은 대조구와 유의적 차이가 없었으나 다른 시험구들은 대조구보다 적었다(P<0.05). 22일령부터 49일령가지의 증체량은 T1구가 대조구와 유의적인 차이는 없었으나 가장 높은 증체를 보였다. 전기간인 7일령부터 49일령 까지의 증체량과 49일령 체중도 T1구만 대조구와 유의적인 차이가 없었고 다른 시험구들은 대조구보다 적었다(P<0.05) .제한사양후부터 21일령까지의 사료섭취량은 T1구를 제외한 나머지 시험구에서는 대조구에 비해 유의적으로 적었으며 (P<0.05) , 전기간 셥취량도 T1구만 대조구와 유의적인 차이를 보이지 않았으나, 희석사료섭취기간의 왕겨섭취량을 제외한 육계사료 섭취량에서는 모든 시험구들이 대조구에 비해 유의적으로 적게 섭취하였다(P<0.05) 제한사양기간을 포함한 7일령부터 21일령까지의 사료요구율은 T1구와 T2구가 대조구에 비해 유의적으로 높았으며(P<0.05), 22일령부터 49일령가지의 사료요구율은 대조구에 비해 모든 시험구들이 유의적으로 낮았으며(P<0.05), 전기간동안의 사료요구율은 T1구를 제외한 나머지 시험구들은 대조구에 비해서 유의적으로 낮았으나(P<0.05) , 희석사료섭취기간의 왕겨섭취 량을 제외 한 육계 사료요구율은 대조구에 비해서 모든 시험구들이 유의적으로 낮거나(P<0. 05) 낮아지는 경향을 보였다. 복강내 지방함량 및 전기간 폐사율은 처리간에 유의적 차이가 없었다. This experiment was conducted to study the effect of early-life feed restriction with diet dilution on compensatory growth, feed efficiency and abdominal fat pad deposition in broilers. In this study, the chicks were randomly assigned to five treatments. Twenty chicks were assigned to each floor pen, and each dietary treatment was replicated with three pens. Birds In control group (C) were full-fed a starter diet throughout the experimental period, and all birds in four dietary treatments (T1-T4) were fed as starter diet diluted with 50% rice hulls. Birds in T1 were fed with a diluted starter diet ad libitum from 7- to 74-d. In T2,the feeding program was 1-d withdrawal alternating with 3-d feed and in T3 1-d withdrawal alternating with 2-d feed. The feeding Program in T4 was alternate days withdrawal and feeding. The feeding with the diluted starter diet (T1) did not significantly affect to growth rate as compared to the birds of C. When periods of 24 h feed withdrawal were Imposed in conjunction with the diluted diets, birds were under weight at 49 d. As the diluted diet treatment was combined with feed withdrawal (T2-T4), there were further less growth. During the 22 to 49 d Period,T1 birds had greater weight gain compared to other treatments (P<0.05) . Birds consumed less feed from 7- to 49-d when the rice hull dilution was used, and this effect was increased by imposition of feed withdrawal (P<0.07) . If rice hull was excluded from the calculation of feed intake (assumed indigestible) then Intake of the starter diet was markedly less for restricted vs. control birds. After 22 d and from 7 to 49 d, restricted birds had superior feed conversion (P<0.05) compared to control birds. Abdominal fat pad deposition and mortality were not influenced by early feed restriction by diluted diet (P<0.05) .

Upregulated heat shock protein beta-1 associated with caloric restriction and high feed efficiency in longissimus dorsi muscle of steer

Jung, U.S.,Kim, M.J.,Wang, T.,Lee, J.S.,Jeon, S.W.,Jo, N.C.,Kim, W.S.,Baik, M.,Lee, H.G. ELSEVIER SCIENCE B.V. AMSTERDAM 2017 LIVESTOCK SCIENCE Vol. No.

The objective of this study was to identify myogenic proteins associated with caloric restriction and feed efficiency in bovine longissimus dorsi muscle. Thirty-one Korean native steers were allocated to 100% ad libitum (n = 16) or 80% of ad libitum (n = 15) groups. Regardless of nutritional level, a subset of these animals were assigned to groups with high or low feed efficiency (n = 5) at a later time point based on feed efficiency. A total of 7 differentially expressed proteins were found between groups with different nutrition levels while a total of 12 differentially expressed proteins were found between groups with different feed efficiencies. Interestingly, heat shock protein beta-1 (HSPB1) was a differentially expressed protein that showed up in both results (nutrition level and feed efficiency). It was up-regulated in both the 80% ad libitum group and the high feed efficiency group. In in vitro study, mRNA expression level of HSPB1 was increased (P < 0.05) after myogenic differentiation. Results of this study suggest that HSPB1 might be a myogenic protein involved in response to caloric restriction and feed efficiency in longissimus dorsi muscle of Korean native steer.

Effect of probiotic 379D as an alternative to antibiotics for feed supplementation in broiler chickens

Se Eun Kim,Kyung Mi Shim,Jong Choon Kim,Yun Hee Choi,Jin Cheol Yoo,Seong Soo Kang 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.1

Antibiotics have been used to prevent disease, promote growth rate, and improve feed efficiency. However, the use of antibiotics in livestock has been restricted worldwide due to problems such as bacterial resistance. Therefore, probiotics among alternatives to antibiotics have gained attention in the livestock feed industry these days. This study was conducted to investigate the effects of dietary supplementation with probiotic 379D on safety, growth rate, and feed efficiency. In this study, bacterial strain 379D was isolated from soil and identified as a Bacillus sp. according to 16S rRNA sequence analysis. In an in vitro test, in-gel activity assay and antimicrobial susceptibility test were conducted to evaluate 379D. In an in vivo study, 379D was administered at concentrations of 0.1% and 1% to broiler chickens for 28 days. The results of in-gel activity assay and antimicrobial susceptibility test showed that strain 379D had broad spectrum antimicrobial activity. Furthermore, no adverse 379D-related effects were observed in 0.1% and 1% groups. Feed efficiency was higher in the 379D-treated groups than in the control group. In conclusion, 379D is expected to be used as a safe alternative to antibiotics in a feed supplement and will improve feed efficiency in broiler chickens.

STUDIES ON THE NUTRIENT REQUIREMENTS OF KOREAN NATIVE CATTLE I. EFFECT OF ENERGY LEVELS ON PERFORMANCE AND ENERGY INTAKE OF GROWING AND FINISHING KOREAN NATIVE CATTLE

Ahn, B.H.,Ahn, D.W. Asian Australasian Association of Animal Productio 1989 Animal Bioscience Vol.2 No.2

Growing and finishing Korean native bulls were used to investigate the effects of different dietary energy levels on performance and energy intake. Experimental observations were made over three weight categories (200 to 250 kg, 250 to 350 kg and 350 to 450 kg). Three diets (2.4, 2.6 and 2.8 Mcal ME/kg DM) were used for each weight category. Crude protein levels of the diets were 12, 11 and 10% for the respective weight groups. Dietary energy level did not significantly affect daily body gain within a weight range but daily body gain during the entire experiment was higher (P<.05) in bulls receiving 2.6 Mcal energy diet than in those fed 2.4 and 2.8 Mcal energy diets. The following equation was developed to predict daily body gain(Y) from energy levels(X) of ration. $Y\;=\;1.3.475X\;-\;2.5949X^{2}\;-\;16.355$ Increasing energy levels significantly (P<.05) decreased daily feed intake. The following equation was developed to predict daily feed intake(Y) from energy levels(X) of ration. $Y\;=\;-30.013X\;+\;5.4401X^{2}\;+\;49.119$ Feed intake per metabolic body size during the entire feeding period ranged from 100.9 to 110.8 g and was lower in bulls fed 2.6 and 2.8 Mcal energy diets than in those fed 2.4 Mcal energy diet. Increasing energy levels significantly (P<.05) improved feed efficiency. The following equation was developed to predict feed efficiency(Y) from energy levels(X) of ration. $Y\;=\;-118.34X\;+\;22.448X^{2}\;+\;162.85$ Daily energy intake during the entire experiment ranged from 18.90 to 19.99 Mcal and there was no significant difference among energy levels. Daily energy intake per metabolic body size during the feeding period ranged from 248.6 to 260.8 kcal and was slightly higher in bulls receiving 2.8 Mcal than in those fed 2.4 and 2.6 Mcal energy diets. Energy required per kg body gain ranged from 17.25 to 19.11 Mcal and was slightly lower in bulls receiving 2.6 Mcal energy diet than in those fed 2.4 and 2.8 Mcal energy diets.

어류 양식업에 있어서 생사료와 배합사료 급이방식의 경영성과 비교분석

송정헌(Jung-Hun SONG) 한국수산해양교육학회 2011 水産海洋敎育硏究 Vol.23 No.3

The formula feed has been valuated its superiority in the aquaculture industry. However, the fish farmer is preferred the raw feed than the formula feed yet. The objectives of this study are to clarified the reason of lower usage of formula feed in aquaculture. We referred to the literature and the enquete, and inspected on-site for this study. Two types of managements, formula feed-usage or not, were compared and analyzed. The results show that the perception of formula feed are changing even though the quality of formula feed is not clear and the growth efficiency is lower than raw feed, because the domestic supply of raw feed was not smooth and the cost was raised.

Comparative Study on 220V AC Feed System and 300V DC Feed System for Internet Data Centers

Hyosung Kim 전력전자학회 2012 JOURNAL OF POWER ELECTRONICS Vol.12 No.1

Internet Data Centers (IDCs), which are essential facilities in the modern IT industry, typically have scores of MW of concentrated electric loads. The provision of an Uninterruptible Power Supply (UPS) is necessary for the power feed system of IDCs owing to the need for stable power. Thus, conventional IDC AC power feed systems have three cascaded power conversion stages, (AC-DC), (DC-AC), and (AC-DC), resulting in a very low conversion efficiency. In comparison, DC power feed systems require only a single power conversion stage (AC-DC) to supply AC main power to DC server loads, resulting in comparatively high conversion efficiency and reliability [4-11]. This paper compares the efficiencies of a 220V AC power feed system with those of a 300V DC power feed system under equal load conditions, as established by the Mok-Dong IDC of Korea Telecom Co. Ltd. (KT). Experimental results show that the total operation efficiency of the 300V DC power feed system is approximately 15% higher than that of the 220V AC power feed system.

Comparative Study on 220V AC Feed System and 300V DC Feed System for Internet Data Centers

김효성 전력전자학회 2012 JOURNAL OF POWER ELECTRONICS Vol.12 No.1

Internet Data Centers (IDCs), which are essential facilities in the modern IT industry, typically have scores of MW of concentrated electric loads. The provision of an Uninterruptible Power Supply (UPS) is necessary for the power feed system of IDCs owing to the need for stable power. Thus, conventional IDC AC power feed systems have three cascaded power conversion stages, (AC-DC), (DC-AC), and (AC-DC), resulting in a very low conversion efficiency. In comparison, DC power feed systems require only a single power conversion stage (AC-DC) to supply AC main power to DC server loads, resulting in comparatively high conversion efficiency and reliability [4-11]. This paper compares the efficiencies of a 220V AC power feed system with those of a 300V DC power feed system under equal load conditions, as established by the Mok-Dong IDC of Korea Telecom Co. Ltd. (KT). Experimental results show that the total operation efficiency of the 300V DC power feed system is approximately 15% higher than that of the 220V AC power feed system.