염교 및 함유 Dimethyl Disulfide의 식중독 노로바이러스 Surrogate인 Murine Norovirus 저해활성

정미숙 한국식품조리과학회 2018 한국식품조리과학회지 Vol.34 No.2

Purpose: Norovirus is the most common cause of human acute gastroenteritis and is the major pathogen of foodborne viral disease. In this study, survival of a foodborne viral surrogate, murine norovirus, was investigated on Allium chinense as well as one of its components, dimethyl disulfide. Methods: The inhibitory effects of A. chinense and its dimethyl disulfide against a foodborne viral surrogate, murine norovirus-1 (MNV), were investigated using time-of-addition plaque assay. Pre-virus treatment and co-treatment were designed to determine the inhibitory mechanism of A. chinense and its dimethyl disulfide against MNV. Results: Inhibitory effects were observed upon pre-virus treatment and co-treatment with A. chinense in a dose-dependent manner. Dimethyl disulfide, a major flavor component of A. chinense, showed inhibitory effects in the pre-virus treatment and co-treatment. Conclusion: A. chinense and its dimethyl disulfide showed inhibitory effects against the foodborne viral surrogate, MNV. Therefore, A. chinense may be a food material candidate for control of foodborne viral disease.

통계모델을 이용한 Biosolids에서 발생하는 Dimethyl Disulfide 농도 예측에 관한 연구

김현욱,김재혁,최홍림 한국냄새환경학회 2005 실내환경 및 냄새 학회지 Vol.4 No.2

Two statistical models were used to predict the concentration of dimethyl disulfide (DMDS) released from biosolids produced by an advanced wastewater treatment plant (WWTP) located in Washington DC, USA. DMDS, is known to contribute to biosolids odors. These models identified oxidation/reduction potential (ORP) values of a gravity thickeners (GT) and dissolved air flotation (DAF), the amount of sludge dewatered by centrifuges, and the blend ratio between GT thickened sludge and DAF thickened sludge in blending tanks as control variables. The accuracy of the developed regression models was evaluated by checking the adjusted R2 of the regression as well as the signs of coefficients associated with each variable. 하수처리과정의 부산물인 슬러지로부터 배출되는 dimethyl disulfide (DMDS)의 농도를 예측하기 위한 두 가지 통계모델을 개발하였다. DMDS는 슬러지에서 악취를 유발하는 주요 화합물로 알려져 있다. 개발된 두개의 통계모델들은 중력침강조와 공기부상조에서의 산화환원전위 (Oxidation Reduction Potential: ORP), 원심분리기 (Centrifuges)에서 탈수된 슬러지 총량 및 혼화조 (Blend tank)로 유입되는 공기부상조와 중력침강조의 농축 슬러지 혼합비율을 변수로 사용하고 있다. 각각의 변수에 관계되는 계수의 부호와 함께 조정된 결정계수 (R2)를 이용하여 개발된 통계모델의 유효성을 평가하였다.

Determination of Dimethyl Disulfide, Diallyl Disulfide, and Diallyl Trisulfide in Biopesticides Containing Allium Sativum Extract by Gas Chromatography

( Sung Jin Lim ),( Ji Hye Lee ),( Jin Hyo Kim ),( Geun Hyoung Choi ),( Nam Jun Cho ),( Byung Jun Park ) 한국환경농학회 2014 한국환경농학회지 Vol.33 No.4

BACKGROUND: Garlic (Allium sativum) extract has been allowed as commercial biopesticide material for pesticidal activity in the Environmentally-friendly Agriculture Promotion Act. Nine commercial biopesticides containing A. sativum extract have been marketed in Korea. However, the analytical method of the active substances in these materials has not been studied. METHODS AND RESULTS: Cartridge clean-up method for the determination of dimethyl disulfide(DMDS), diallyl disulfide(DADS), and diallyl trisulfide(DATS) in biopesticides containing A. sativum extract was developed and validated by gas chromatography(GC). The clean-up method was optimized using hydrophilic lipophilic balance (HLB) solid phase extraction(SPE) cartridges for the bioactive sulfides in biopesticides containing A. sativum extract, and the eluate was analyzed to quantify the DMDS, DADS, and DATS using the GC. The developed method was validated, and the LOQ and recovery rates of DMDS,DADS, and DATS were 0.226, 0.063, and 0.051 mg L-1 and 80.6, 84.8, and 73.1%, respectively. From the nine commercial biopesticide samples, contents of DMDS, DADS, and DATS were analyzed using the developed method and results showed <LOQ, <LOQ-113.4, and <LOQ-2.3 mg L-1, respectively. CONCLUSION: The developed method could be used in determining the quality of biopesticides for the manufacture of commercial biopesticides containing A. sativum extract.

여수 선소(船所) 주변지역의 황화합물 악취 특성

채종철,윤형선,서성규 國立麗水大學校 環境問題硏究所 2004 環境硏究論文集 Vol.6 No.-

The objective method, combined sensory and instrumental analysis, was desired to evaluation of intensity as well as odor active compounds because malodor is sensory pollution. The Seonso area was classified into seaside, residential and reference area. Target compounds were sulfur compounds such as hydrogen sulfide(H₂S). methyl mercaptan(CH₃SH), dimethyl sulfide((CH₃)₂S)and dimethyl disulfide((CH₃)₂S₂). The odor intensity at Seonso area in Yeosu city was set in order as follows : reference area < residential area < seaside area. There was a difference of the odor intensity between the summer and the winter at seaside area. Most of the odor intensity identified at seaside area(S-3) was exceeded the Korean odor regulations(the level 3.0). The concentrations of dimethyl sulfide, dimethyl disulfide were not significantly changed with sampling time and site. However, the concentration of hydrogen sulfide and methyl mercaptan at S-3 site were higher than other sites and these concentrations of the summer were higher than that of the winter. The concentrations of hydrogen sulfide at the sediments of R-1, S-1, S-2, S-3 sites by the Gastec detection tube were 0.16, 1.26, 0.43 and 2.20㎎/g-dry respectively. The order of major odor active compounds related to Seonso area were dimethyl disulfide < dimethyl sulfide < methyl mercaptan < hydrogen sulfide.

Abnormal adsorption behavior of dimethyl disulfide on gold surfaces

Noh, Jaegeun,Jang, Soonmin,Lee, Donghyung,Shin, Seokmin,Ko, Young Joon,Ito, Eisuke,Joo, Sang-Woo Elsevier 2007 Current Applied Physics Vol.7 No.6

<P><B>Abstract</B></P><P>Adsorption of dimethyl disulfide (DMDS) on gold colloidal nanoparticle surfaces has been examined to check its binding mechanism. Differently from previous results, DMDS molecules adsorbed on the gold surface at high concentration showed the S–S stretching band at ∼500cm<SUP>−1</SUP> in surface-enhanced Raman scattering (SERS) spectra, which indicates the presence of intact adsorption of DMDS molecules. However, it was found that the S–S bond of disulfides was easily cleaved on the gold surface at low concentration. These behaviors were not observed for diethyl disulfide (DEDS) or diphenyl disulfide (DPDS). Our results indicate that DMDS molecules with the shortest alkyl chains on the gold surface can be inserted into self-assembled monolayers (SAMs) without the S–S bond cleavage during self-assembly due to insufficient lateral van der Waals interaction and the low adsorption activity of disulfides, whereas DEDS with longer alkyl chains or DPDS with the weak disulfide bond dissociation energy would not. These unusual DMDS adsorption behaviors were examined by scanning tunneling microscopy (STM) and X-ray photoelectron spectroscopy (XPS). We also compared the bonding dissociation energy of the S–S bonds of various disulfides by means of a density functional theory (DFT) calculation.</P>

Determination of Dimethyl Disulfide, Diallyl Disulfide, and Diallyl Trisulfide in Biopesticides Containing Allium Sativum Extract by Gas Chromatography

Lim, Sung-Jin,Lee, Ji-Hye,Kim, Jin-Hyo,Cho, Geun-Hyoung,Cho, Nam-Jun,Park, Byung-Jun The Korean Society of Environmental Agriculture 2014 한국환경농학회지 Vol.33 No.4

BACKGROUND: Garlic (Allium sativum) extract has been allowed as commercial biopesticide material for pesticidal activity in the Environmentally-friendly Agriculture Promotion Act. Nine commercial biopesticides containing A. sativum extract have been marketed in Korea. However, the analytical method of the active substances in these materials has not been studied. METHODS AND RESULTS: Cartridge clean-up method for the determination of dimethyl disulfide(DMDS), diallyl disulfide(DADS), and diallyl trisulfide(DATS) in biopesticides containing A. sativum extract was developed and validated by gas chromatography(GC). The clean-up method was optimized using hydrophilic lipophilic balance (HLB) solid phase extraction(SPE) cartridges for the bioactive sulfides in biopesticides containing A. sativum extract, and the eluate was analyzed to quantify the DMDS, DADS, and DATS using the GC. The developed method was validated, and the LOQ and recovery rates of DMDS, DADS, and DATS were 0.226, 0.063, and $0.051mg\;L^{-1}$ and 80.6, 84.8, and 73.1%, respectively. From the nine commercial biopesticide samples, contents of DMDS, DADS, and DATS were analyzed using the developed method and results showed <LOQ, <LOQ-113.4, and <LOQ-$2.3mg\;L^{-1}$, respectively. CONCLUSION: The developed method could be used in determining the quality of biopesticides for the manufacture of commercial biopesticides containing A. sativum extract.

Determination of Dimethyl Disulfide, Diallyl Disulfide, and Diallyl Trisulfide in Biopesticides Containing Allium Sativum Extract by Gas Chromatography

임성진,이지혜,김진효,최근형,조남준,박병준 한국환경농학회 2014 한국환경농학회지 Vol.33 No.4

BACKGROUND: Garlic (Allium sativum) extract has beenallowed as commercial biopesticide material for pesticidalactivity in the Environmentally-friendly AgriculturePromotion Act. Nine commercial biopesticides containingA. sativum extract have been marketed in Korea. However,the analytical method of the active substances in thesematerials has not been studied. METHODS AND RESULTS: Cartridge clean-up methodfor the determination of dimethyl disulfide(DMDS), diallyldisulfide(DADS), and diallyl trisulfide(DATS) inbiopesticides containing A. sativum extract was developedand validated by gas chromatography(GC). The clean-upmethod was optimized using hydrophilic lipophilic balance(HLB) solid phase extraction(SPE) cartridges for thebioactive sulfides in biopesticides containing A. sativumextract, and the eluate was analyzed to quantify the DMDS,DADS, and DATS using the GC. The developed methodwas validated, and the LOQ and recovery rates of DMDS,DADS, and DATS were 0.226, 0.063, and 0.051 mg L-1 and80.6, 84.8, and 73.1%, respectively. From the ninecommercial biopesticide samples, contents of DMDS,DADS, and DATS were analyzed using the developedmethod and results showed <LOQ, <LOQ-113.4, and<LOQ-2.3 mg L-1, respectively. CONCLUSION: The developed method could be used indetermining the quality of biopesticides for themanufacture of commercial biopesticides containing A. sativum extract.

Head-space GC-MS를 활용한 마늘추출물 함유 유기농자재 중 Allylmethyl Sulfide, Dimethyl Disulfide 및 Dipropyl Sulfide 분석

임성진 ( Sungjin Lim ),오영탁 ( Youngtak Oh ),김진효 ( Jinhyo Kim ),최근형 ( Geunhyoung Choi ),박병준 ( Byungjun Park ) 한국환경농학회 2015 한국환경농학회지 Vol.34 No.3

BACKGROUND: Garlic (Allium sativum) contains polyphenols and sulfur compounds that are recognized as antioxidant, antithrombotic, anticancer, antibacterial, antimicrobial, nematicidal, and insecticidal activity. For this reason, the Environmentally-friendly Agriculture Promotion Act allowed the garlic extract as commercial biopesticide material for crop protection, nine commercial biopesticides containing A. sativum extract have been marketed in Korea. METHODS AND RESULTS: The determination of allylmethyl sulfide (AMS), dimethyl disulfide (DMDS), and dipropyl sulfide (DPS) in biopesticides containing A. sativum extract was developed and validated by gas chromatography (GC) mass spectrometry (MS) with head-space sampler. The developed method was validated, and the limit of quantification (LOQ) and recovery rates of AMS, DMDS, and DPS were 0.08, 0.32, and 0.09 mg/L and 90.3-91.3, 86.2-88.3, and 87.6-89.5%, respectively. From the nine commercial biopesticide samples, contents of AMS, DMDS, and DPS were analyzed using the developed method and results showed <LOQ, <LOQ-20.0 mg/L, and <LOQ, respectively. CONCLUSION: The developed method would contribute to manufactures producing commercial biopesticides and the quality control of biopesticides containing A. sativum extract.

Dimethyl Disulfide (DMDS) as a Potential Biopesticide and Alternative to Methyl Bromide against Root Lesion Nematodes (Pratylenchus spp.), Root Knot Nematodes (Meloidogyne spp.) and Potato Cyst Nematodes (Globodera spp.) on Lily and Potato

Coosemans Jozef,Du Yu 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Vesicles Comprising Dimethylaminopropyl Octadecanamide, Stearic Acid, and Carboxyhexadecyl Disulfide and Their Release Property under Reducing Condition

김진아,김진철 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.5

Vesicles comprising dimethyl aminopropyl octadecanamide (DMAPODA), stearic acid (SA), and carboxyhexadecyl disulfide (CHDDS) were prepared as reduction-responsive carriers. Vesicular particles were found on TEM micrographs and the diameter fell within 100-400 nm. The hydrodynamic mean diameter increased with increasing the content of CHDDS. The zeta potential was lowered by the incorporation of CHDDS into the vesicular membrane. Vesicle whose DMAPODA/SA/ CHDDS molar ratio was 1/1/5 exhibited one endothermic peak around 50.9°C, indicating that the vesicular membrane was homogeneous in the composition. Strong signals were found in 500-540 cm-1 in the Raman spectrum of vesicles, suggesting that the disulfide lipid (i.e. CHDDS) was incorporated into the vesicular membranes. Vesicles containing CHDDS released rhodamine B in dithiothreitol (DTT, a reducing agent) concentration-dependent manner. The DTTsensitivity of the vesicles in terms of release was higher as the CHDDS content was higher.