Effect of autologous platelet-rich plasma application on cutaneous wound healing in dogs

Cho-Hee Jee,Na-Young Eom,Hyo-Mi Jang,Hae-Won Jung,Eul-Soo Choi,Jin-Hee Won,Il-Hwa Hong,Byeong-Teck Kang,Dong Wook Jeong,Dong-In Jung 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1

The present study was conducted to identify the effectiveness of platelet-rich plasma (PRP) and efficacy of intralesional injection as a method of application to acute cutaneous wounds in dogs. Healthy adult beagles (n = 3) were used in the present study. Autologous PRP was separated from anticoagulant treated whole blood in three dogs. Cutaneous wounds were created and then treated by intralesional injection of PRP in the experimental group, while they were treated with saline in the control group on days 0, 2 and 4. The healing process was evaluated by gross examination throughout the experimental period and histologic examination on day 7, 14 and 21. In PRP treated wounds, the mean diameter was smaller and the wound closure rate was higher than in the control. Histological study revealed that PRP treated wounds showed more granulation formation and angiogenesis on day 7, and faster epithelialization, more granulation formation and collagen deposition were observed on day 14 than in control wounds. On day 21, collagen deposition and epithelialization were enhanced in PRP treated groups. Overall, PRP application showed beneficial effects in wound healing, and intralesional injection was useful for application of PRP and could be a good therapeutic option for wound management in dogs.

양서류 피부 상처회복과정 중 각질화세포 cytokeratin의 분포

임도선,정순정,문명진,정문진,Lim, Do-Seun,Jeong, Soon-Jeong,Moon, Myung-Jin,Jeong, Moon-Jin Korean Society of Electron Microscopy 2007 Applied microscopy Vol.37 No.4

The system of wound healing is very complex biological processing that includes inflammatory, reepithelialization, and matrix construction. For identification of the transitional pathway of the keratinocytes, we have employed immunohistochemical analysis using cytokeratin antibody after wounding. Epithelium in skin of the frog(Bombina orientalis) was examined with transmission electron microscopy. Cytokeratin was expressed in normal basal and gland cavity cells. At 3-hour basal layer cells were strong positive, however cells of the upper layer were negative reaction. Day1 and 2 after post-wounding, regenerating epithelial cell layer was positive reaction, especially basal layer cells were strong positive. At day 10 after wounding, the degree of positive reaction to basal cells of regenerating epithelial tissue was equal to day 7 wound tissue. At day of 19th, basal and spinous layer cells were strong positive reaction. Regenerating epithelial cells were positive but some basal cells were strong positive at day 27. From this result, we identified that the migration of the keratinocytes in amphibian skin wounds is initiated from basal layer fells and the keratinocytes migrate into basal and middle of the wound area. 상처회복은 염증, 재상피화 그리고 기질의 재형성등이 관여하는 복잡한 과정이다. 이중 재상피화에 관여하는 각질화세포의 이동경로를 분석하기 위하여 무당개구리 피부 상처유도 후 투과전자현미경과 cytokeratin에 대한 조직면역화학법을 이용하였다. 정상조직의 cytokeratin발현은 기저층의 세포들과 선상피에서 확인되었다. 상처 유도후 3시간 조직에서, 기저세포층에서 강한 반응이 관찰되었고, 1일과 2일 사이에서는 재생되는 각질화세포에서 강한 면역반응이 확인되었다. 상처반응 중기인 7일부터 10일 사이에서도 재생된 세포의 기저층세포에서 강한 반응이 일어났다. 19일경과의 조직에서는 기저층과 유극층의 세포들에서 cytokeratin의 발현이 증가하였다. 따라서, 재상피화에 관여하는 각질화세포는 기저층의 세포로부터 시작하여 상처부위로 이동하여 과립층과 유극층으로 분화됨으로서 재상피가 진행되었다.

구강암 수술 후 발생한 구강 경부 누공(orocutaneous fistula) 치료 시 진공 음압 폐쇄(vacuum-assisted closure) 시스템을 이용한 상처 치료

이승준(Seung-June Lee),권진일(Jin-il Kwon),임경민(Kyung-Min Lim),김형준(Hyung Jun Kim),차인호(In-Ho Cha),남웅(Woong Nam) 대한구강악안면외과학회 2010 대한구강악안면외과학회지 Vol.36 No.5

Fleischmann et al. first described the concept of using sub-atmospheric pressure to treat open or infected wounds in 1993. Since then, Argenta and Morykwas developed subatmospheric, or negative pressure dressings in 1997 as a means of managing complicated wounds. Since its introduction in 1997, the vacuum-assisted closure (VAC) system has been used widely in general plastic surgery, general surgery, and orthopedic surgery to manage complicated wounds of the torso and extremities. However, there is a paucity of literature describing its use in the head and neck region, particularly in oral and maxillofacial surgery. We report a successful case of postoperative orocutaneous fistula closure using a VAC system in a 59-year male with a review of the relevant literature.

Fabricating Composite Cell Sheets for Wound Healing: Cell Sheets Based on the Communication Between BMSCs and HFSCs Facilitate Full-Thickness Cutaneous Wound Healing

Li Gongjian,Wang Qin,Liu Hao,Yang Zuojun,Wu Yuhan,He Li,Deng Xiaoyuan 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.3

Background: Insufficient angiogenesis and the lack of skin appendages are critical challenges in cutaneous wound healing. Stem cell-fabricated cell sheets have become a promising strategy, but cell sheets constructed by a single cell type are inadequate to provide a comprehensive proregenerative microenvironment for wound tissue. Methods: Based on the communication between cells, in this study, bone marrow mesenchymal stem cells (BMSCs) and hair follicle stem cells (HFSCs) were cocultured to fabricate a composite cell sheet (H/M–CS) for the treatment of full-thickness skin wounds in mice. Results: Experiments confirmed that there is cell–cell communication between BMSCs and HFSCs, which enhances the cell proliferation and migration abilities of both cell types. Cell–cell talk also upregulates the gene expression of pro-angiogenic-related cytokines in BMSCs and pro-hair follicle-related cytokines in HFSCs, as well as causing changes in the properties of secreted extracellular matrix components. Conclusions: Therefore, the composite cell sheet is more conducive for cutaneous wound healing and promoting the regeneration of blood vessels and hair follicles. Background: Insufficient angiogenesis and the lack of skin appendages are critical challenges in cutaneous wound healing. Stem cell-fabricated cell sheets have become a promising strategy, but cell sheets constructed by a single cell type are inadequate to provide a comprehensive proregenerative microenvironment for wound tissue. Methods: Based on the communication between cells, in this study, bone marrow mesenchymal stem cells (BMSCs) and hair follicle stem cells (HFSCs) were cocultured to fabricate a composite cell sheet (H/M–CS) for the treatment of full-thickness skin wounds in mice. Results: Experiments confirmed that there is cell–cell communication between BMSCs and HFSCs, which enhances the cell proliferation and migration abilities of both cell types. Cell–cell talk also upregulates the gene expression of pro-angiogenic-related cytokines in BMSCs and pro-hair follicle-related cytokines in HFSCs, as well as causing changes in the properties of secreted extracellular matrix components. Conclusions: Therefore, the composite cell sheet is more conducive for cutaneous wound healing and promoting the regeneration of blood vessels and hair follicles.

A Disposable Photovoltaic Patch Controlling Cellular Microenvironment for Wound Healing

Jang, Hyeon-Ki,Oh, Jin Young,Jeong, Gun-Jae,Lee, Tae-Jin,Im, Gwang-Bum,Lee, Ju-Ro,Yoon, Jeong-Kee,Kim, Dong-Ik,Kim, Byung-Soo,Bhang, Suk Ho,Lee, Tae Il MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.10

<P>Electrical stimulation (ES) is known to affect the wound healing process by modulating skin cell behaviors. However, the conventional clinical devices that can generate ES for promoting wound healing require patient hospitalization due to large-scale of the extracorporeal devices. Herein, we introduce a disposable photovoltaic patch that can be applied to skin wound sites to control cellular microenvironment for promoting wound healing by generating ES. In vitro experiment results show that exogenous ES could enhance cell migration, proliferation, expression of extracellular matrix proteins, and myoblast differentiation of fibroblasts which are critical for wound healing. Our disposable photovoltaic patches were attached to the back of skin wound induced mice. Our patch successfully provided ES, generated by photovoltaic energy harvested from the organic solar cell under visible light illumination. In vivo experiment results show that the patch promoted cutaneous wound healing via enhanced host-inductive cell proliferation, cytokine secretion, and protein synthesis which is critical for wound healing process. Unlike the current treatments for wound healing that engage passive healing processes and often are unsuccessful, our wearable photovoltaic patch can stimulate regenerative activities of endogenous cells and actively contribute to the wound healing processes.</P>

Effects of electrolyzed strong acidic water on wound healing via immune-redox mechanism

Ailyn Fadriquela,Ma Easter Joy Sajo,Johny Bajgai,Jesmin Ara,Soo Ki Kim,Kyu Jae Lee 한국물학회 2017 한국물학회지 Vol.6 No.1

Acidic water is known to inactivate microbes, fungus, viruses, toxins, is used for disinfecting food equipments, vegetables, fruits, poultry and meat. Some studies were also done to show its effect in wound healing. However, the mechanism is not fully understood. This study aimed to unravel the mechanism behind the wound healing effect of strong electrolyzed acidic water (SEAW). The study was conducted to identify the effect of SEAW on cutaneous wounds using a punch removing 5mm skin plug resulting in elliptical wound (Disposable Biopsy Punch, Integra-Miltex, PA, USA). SEAW was generated from a device (HDR Co. Bucheon, Korea), with characteristics of having pH from 5.0 to 6.5, ORP around 800 mV and chlorine concentration of 10 to 30 mg/L. SEAW was sprayed daily for 7 days to the cutaneous wounds of hairless mice and was compared to other treatments such as saline, alcohol and betadine treated groups. Wound areas were measured and digital photographs were conducted daily. Serum and skin lysate were collected on the seventh day, thereafter, reactive oxygen species (ROS) and nitric oxide (NO) assays, oxidative stress assays, and matrix metalloproteinases (MMPs) expressions were analyzed. The results showed that among all groups, SEAW had the highest wound size reduction especially on day 4. ROS in serum was significantly lower than other groups and ROS in skin lysate was lower and comparable to the positive control groups. SEAW has the highest NO activity among all groups which may mean that SEAW can have the most significant action on remodelling stage through the production of NO, and through phagocytosis. Consistently, antioxidant assays of skin lysate shows that GPx, Catalase and MPO activities group treated with SEAW compensate the total ROS in skin. Immunoblotting of matrix metalloproteinases (MMP1 and MMP9), the enzymes responsible for extracellular matrix degradation, were also upregulated in SEAW treated group. Collectively, it is shown that comparable to potent medicine in wound healing such as betadine and alcohol, strong acidic water is similarly effective in wound healing and is shown to act through the immune defense and upregulation of enzymes such as MMPs. However, more studies are still need to be done to confirm these mechanisms.

Ginsenoside Rb1 Enhances Keratinocyte Migration by a Sphingosine-1-Phosphate-Dependent Mechanism

신경오,최성재,Yoshikazu Uchida,김인용,정윤화,박경호 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.11

The cutaneous wound healing process is tightly regulated by a range of cellular responses, including migration. Sphingosine-1-phosphate (S1P) is a signaling lipid produced in keratinocytes (KC) and it is known to stimulate skin wound repair through increased KC migration. Of the multifunctional triterpene ginsenosides, Rb1 enhances cutaneous wound healing process by increasing KC migration, but cellular mechanisms responsible for the Rb1-mediated increase in KC migration are largely unknown. Therefore, we hypothesized that, and assessed whether, Rb1 could stimulate KC migration through S1P-dependent mechanisms. Rb1 significantly increases S1P production by regulating the activity of metabolic conversion enzymes associated with S1P generation and degradation, sphingosine kinase 1 (SPHK1) and S1P lyase, respectively, in parallel with enhanced KC migration. However, blockade of ceramide to S1P metabolic conversion using a specific inhibitor of SPHK1 attenuated the expected Rb1-mediated increase in KC migration. Furthermore, a pan-S1P receptor inhibitor pertussis toxin significantly attenuated Rb1-induced stimulation of KC migration. Moreover, the Rb1-induced increases in KC migration required S1P receptor(s)-mediated activation of ERK1/2 and NF-κB, leading to production of key cutaneous migrating proteins, matrix metalloproteinase (MMP)-2 and MMP-9. Taken together, the results show that Rb1 stimulates KC migration through an S1P→S1P receptor(s)→ERK1/2→NF-κB→MMP-2/-9 pathway. This research revealed a previously unidentified cellular mechanism for Rb1 in enhancing KC migration and pointing to a new therapeutic approach to stimulate the cutaneous wound healing process.

Enhanced skin wound regeneration using solar cell-based patch

송지훈,방석호 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-

In this study, we introduced a solar cell-based patch that can be applied to skin wound sites to control cellular microenvironment for promoting wound healing by generating electrical stimulation (ES). Our experiment results showed that exogenous ES could enhance cell migration, expression of extracellular matrix proteins, myoblast differentiation, host-inductive cell proliferation, cytokine secretion, and protein synthesis which is critical for wound healing process. Unlike the current treatments for wound healing that engage passive healing processes and often are unsuccessful, our wearable solar cell-based patch can stimulate regenerative activities of endogenous cells and actively contribute to the wound healing processes.

흉부의 둔상 이후 고정된 결절성 병변 형태로 발생한 흉부 피부 누공 1예

이선화 ( Seon Hwa Lee ),박경덕 ( Kyung Duck Park ),장용현 ( Yong Hyun Jang ),이원주 ( Weon Ju Lee ),이석종 ( Seok-jong Lee ),김준영 ( Jun Young Kim ) 대한피부과학회 2021 大韓皮膚科學會誌 Vol.59 No.3

An 83-year-old man presented with an intermittently bleeding fixed nodule on the left side of the chest for 2 months. Due to the clinical suspicion of metastatic cancer or a ruptured epidermal cyst, a punch biopsy was performed, which revealed granulation tissue. During surgical excision, a connection between the granulation tissue and the deeper structure was confirmed. Based on the clinicopathological findings, a cutaneous fistula was suspected; a detailed history revealed that he had experienced blunt chest trauma in a motorcycle accident 2 months before. Subsequent chest computed tomography showed soft tissue infiltration in the left sixth rib. Finally, the patient was diagnosed with a cutaneous fistula and underwent resection of the left sixth rib and costal cartilage. Cutaneous fistulae rarely occur in the chest. In cases where a recalcitrant inflammatory nodule is observed on the chest, the patient’s trauma history and ordinary medical history should be evaluated to rule out a cutaneous fistula. (Korean J Dermatol 2021;59(3):229∼233)

Asiaticoside enhances normal human skin cell migration, attachment and growth in vitro wound healing model

Lee, J.H.,Kim, H.L.,Lee, M.H.,You, K.E.,Kwon, B.J.,Seo, H.J.,Park, J.C. G. Fischer 2012 Phytomedicine Vol.19 No.13

Wound healing proceeds through a complex collaborative process involving many types of cells. Keratinocytes and fibroblasts of epidermal and dermal layers of the skin play prominent roles in this process. Asiaticoside, an active component of Centella asiatica, is known for beneficial effects on keloid and hypertrophic scar. However, the effects of this compound on normal human skin cells are not well known. Using in vitro systems, we observed the effects of asiaticoside on normal human skin cell behaviors related to healing. In a wound closure seeding model, asiaticoside increased migration rates of skin cells. By observing the numbers of cells attached and the area occupied by the cells, we concluded that asiaticoside also enhanced the initial skin cell adhesion. In cell proliferation assays, asiaticoside induced an increase in the number of normal human dermal fibroblasts. In conclusion, asiaticoside promotes skin cell behaviors involved in wound healing; and as a bioactive component of an artificial skin, may have therapeutic value.