Coxiella burnetii감염과 가와사끼병과의 관련성에 관한 연구

김종욱,이해용,이원영,Kim, Jong Wook,Lee, Hae Yong,Lee, Won Young 대한소아감염학회 1996 Pediatric Infection and Vaccine Vol.3 No.2

Purpose : Coxiella burnetii is a well-known causative agent of granuolmatous inflammation and an inducer of morphological change and transformation of human B lymphocyte in vitro. Coxiella burnetii manifests with several clinical symptoms depending upon the organs that are involved. We therefore undertook to clarify the association of Kawasaki disease and Coxiella burnetii. Subjects and Methods : The patient's sera were tested for antibodies specific for Coxiella burnetii, using indirect fluorescent antibody technique(IFA). We compared Coxiella burnetii infection with 3 groups of patients, group 1 (Kawasaki disease), group 2 (other febrile disease) and group 3 (control group). Results : 1) In children with Kawasaki disease (group 1), 93% of the patient tested positive for Coxiella burnetii. 2) In group 2 children, 20% of the patient tested positive for Coxiella burnetii. 3) In group 3 children, 10% of the patient tested positive for Coxiella burnetii 4) There were significant higher positive rate for Coxiella burnetii in Kawasaki disease than group 2 and group 3 (p<0.05). Conclusions : We concluded that our cases of Kawasaki disease were associated with Coxiella burnetii infection. Further studies will be needed to understand the precise role of Coxiella infection in Kawasaki disease.

Coxiella burnetii 감염에 의한 Q fever의 실험실 진단(1)

박만석,심수경,이혜경,김혜리,이병철,박미연 국립보건연구원 2000 국립보건원보 Vol.37 No.-

Detection of Coxiella burnetii in Cattle

김요한,김두 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.6

Coxiella burnetii is an obligate intracellular rickettsial organism and the causative agent of Query fever, a zoonosis that occurs worldwide. In Korea, C. burnetii infection had occurred in humans and animals. However, the studies were only conducted in geographically limited area for detection of C. burnetii. The objective of this study was to detect C. burnetii in Korean native cattle and dairy cattle nationwide by real-time PCR. The total of 807 blood samples from 622 Korean native cattle and 185 dairy cows, 170 individual milk samples of dairy cows, and 348 bulk tank milk samples of dairy herds were collected nationwide. From blood samples, C. burnetii was detected in 17 (2.7%) out of 622 Korean native cattle and 2 (1.1%) of 185 dairy cows. From milk samples, C. burnetii was detected in 27 (15.9%) out of 170 individual milk samples of dairy cows. And C. burnetii was detected in 84 (24.1%) of 348 bulk tank milk samples. In conclusion, this study revealed that the detection rates are considerably high in cattle and the infection of C. burnetii has been continuously occurring in cattle of Korea. In order to prevent the hazards of a zoonosis Q-fever that occur both humans and domestic animals, further studies are needed to clarify the epidemiology of Q-fever of domestic animals and humans in Korea.

광주지역 반추동물의 큐열 항체 보유율 조사

오아름 ( A-reum Oh ),고바라다 ( Ba-ra-da Koh ),정보람 ( Bo-ram Jung ),나호명 ( Ho-myoung Na ),배성열 ( Seong-yeol Bae ),김용환 ( Yong-hwan Kim ) 한국동물위생학회 2021 한국동물위생학회지 (KOJVS) Vol.44 No.1

Q fever is a worldwide zoonotic disease caused by Coxiella burnetii. Domestic ruminants are considered to be major source of human infection. The aim of this survey was to investigate seroprevalence of C. burnetii in ruminants in Gwangju area. A total of 1,000 samples (serum and lactoserum) were collected from 987 Korean native cattle, 5 Korean native goats, 2 beef cattle, 6 bulk-tank milk from each dairy farm in Gwangju area from January to October 2020 and analyzed by ELISA. The seroprevalence of C. burnetii in bulk-tank milk from each dairy farms was 50.0%. Korean black goat and beef cattle had negative antibody test results for C. burnetii. The seroprevalence of C. burnetii in Korean native cattle in Gwangju area was 7.1% and was higher in female (7.8%) than in male (3.4%) (P=0.024). The seroprevalence of C. burnetii in Korean native cattle appeared to increase with age (3.8% in 1 year-old, 7.1% in 3 year-old, and 10.7% in more than 5 year-old) (P<0.001). The seroprevalence of C. burnetii of Korean native cattle increased in spring and May was the highest in particular (P<0.001). As the distribution and density of tick-habitat are expected to increase due to climate crisis, this survey highlights the need for monitoring C. burnetii in domestic ruminants, including surveillance of C. burnetii infection in people working for livestock industry.

Hairy cell transformation of human peripheral blood lymphocytes by Coxiella burnetii

Lee, Won-Young Yonsei University, College of Medicine 1993 Yonsei medical journal Vol.34 No.1

“Hairiness” is a Facsimile of Reorganized Cytoskeletons: A Cytopathic Effect of Coxiella burnetii

이원영 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.10

In 1993, I reported that Coxiella burnetii transforms human B cells into hairy cells (cbHCs), the first hairy cell reported outside ofhairy cell leukemia (HCL). Over last few decades, advances in molecular biology have provided evidence supporting that C. burnetiiinduces hairiness and inhibits the apoptosis of host cells. The present review summarizes new information in support ofcbHC. C. burnetii was shown to induce reorganization of the cytoskeleton and to inhibit apoptosis in host cells. Peritoneal B1acells were found to be permissive for virulent C. burnetii Nine Mile phase I (NMI) strains in mice. C. burnetii severely impaired Ecadexpression in circulating cells of Q fever patients. B-cell non-Hodgkin lymphoma was linked to C. burnetii. Mutation of BRAFV600E was pronounced in HCL, but “hairiness” was not linked to the mutation. Risk factors shared among coxiellosis and HCL inhumans and animals were reported in patients with Q-fever. Accordingly, I propose that C. burnetii induces reorganization of thecytoskeleton and inhibits apoptosis as cytopathic effects that are not target cell specific. The observed hairiness in cbHC appearsto be a fixed image of dynamic nature, and hairy cells in HCL are distinct among lymphoid cells in circulation. As the cytoskeletonplays key roles in maintaining cell structural integrity in health and disease, the pathophysiology of similar cytopathic effectsshould be addressed in other diseases, such as myopathies, B-cell dyscrasias, and autoimmune syndromes.

경북 동부지역 번식장애 소에서의 큐열 항체 보유율

우인옥 ( In Ohk Ouh ),서민구 ( Min Goo Seo ),장영술 ( Young Sul Jang ),김상윤 ( Sang Yun Kim ),곽동미 ( Dong Mi Kwak ) 한국동물위생학회 2013 한국동물위생학회지 (KOJVS) Vol.36 No.4

This study was done to determine the seroprevalence of Coxiella burnetii in cattle with reproductive disorders reared in eastern Gyeongbuk province. A total of 146 cattle blood samples from 83 farms in eastern Gyeongbuk province were collected in 2011∼2012. Among them, 17 (11.6%) samples from 11 (13.3%) farms were seropositive for C. burnetii by ELISA. In dairy cattle with mastitis, 15 (38.5%) milk samples from 9 (42.9%) farms were seropositive among 39 milk samples from 21 farms. Among the seropositive farms, one farm with high seroprevalence was selected to assess the seroprevalence of C. burnetii in the bulk-tank milk (BTM) and blood samples of dairy cattle. The BTM sample from the farm was seropositive to C. burnetii and 6 (15%) out of 40 dairy cattle were seropositive. In cattle with reproductive disorders including infertility, abortion and stillbirth, 2 (1.9%) samples from 2 (3.2%) farms were positive among 107 samples collected from 62 farms. Among 2 positive samples, one of them is dariy cattle (100%) and the other is Korean cattle (0.9%) (P<0.001). This suggests that Korean cattle was relatively resistant to C. burnetii. Based on the data obtained, we identified association between C. burnetii shedding in milk and chronic subclinical mastitis in dairy cattle. Since seroprevalence of C. burnetii is relatively high in dairy cattle, further intensive studies on C. burnetii among the high risk milk farms and dairy cattle herds are needed to evaluate infection status.

경북지역 집합유와 젖소에서 큐열 항체 보유율 조사

우인옥 ( In Ohk Ouh ),서민구 ( Min Goo Seo ),도재철 ( Jae Cheul Do ),김인경 ( In Kyoung Kim ),조민희 ( Min Hee Cho ),곽동미 ( Dong Mi Kwak ) 한국동물위생학회 2013 한국동물위생학회지 (KOJVS) Vol.36 No.4

Q fever is a rickettsial infection caused by Coxiella burnetii that is transmitted from animals to humans. Modes of transmission to humans include inhalation, tick bites and ingestion of unpasteurized milk or dairy products. This survey was aimed at monitoring the seroprevalence of C. burnetii in bulk-tank milk (BTM) in Gyeongbuk province. In addition, the seroprevalence of C. burnetii was investigated at the herd level of dairy cattle in eastern Gyeongbuk province in which many dairy cattle are reared. Among 324 BTM samples collected from 20 country areas, 175 (54%) BTM samples from 15 (75%) country areas were positive for C. burnetii by ELISA. By regions, the seroprevalence of BTM samples in eastern, central, western and northern areas of Gyeongbuk province were 62.7%, 48.4%, 45.1%, and 41.4%, respectively. When analyzed in the dairy cattle reared in the eastern area where high seroprevalence occurred in BTM samples, 119 (24.2%) out of 492 dairy cattle were positive for C. burnetii. Seroprevalence of C. burnetii in dairy cattle was increased with daily milk yield of farm (P<0.05) and age (P<0.001). Since seroprevalence of C. burnetii is relatively high in both BTM samples and dairy cattle reared in Gyeongbuk province, further studies on the high risk farms and herds are needed to evaluate infection status and appropriate control programs in this region.

Seroprevalence of Coxiella burnetii infection in cattle on Ulleung Island, Korea

Seo, Min-Goo,Ouh, In-Ohk,Kim, Young-Hoan,Kim, Joong-Kew,Kwon, Oh-Deog,Kwak, Dongmi The Korean Society of Veterinary Science 2018 大韓獸醫學會誌 Vol.58 No.3

We assessed the seroprevalence of Coxiella burnetii (C. burnetii) in cattle on Ulleung Island, Korea in a population-based follow up study for 4 years and determined the spatial distribution and risk factors associated with C. burnetii. The seroprevalence of C. burnetii was determined to be 1.4-2.0% during 2011-2014. Overall, nine cattle from three farms that tested seropositive showed C. burnetii antibody seroconversions between 2011 and 2014. The number of seropositive cattle was low, suggesting that movement of and contact between animals was possible risk factors for the transmission of C. burnetii.

The Epidemiology of Q fever outbreak at an meat packaging business in South Korea

( Shin Young Lee ),( Seon Do Hwang ),( Jung Yeon Heo ),( Seung Kwan Lim ),( Byoung Hak Jeon ),( En Hi Cho ) 대한인수공통전염병학회 2019 창립총회 및 학술대회 초록집 Vol.2019 No.1

Introduction : Q fever is a zoonosis caused by Coxiella burnetii. A Q fever outbreak occurred among the workers in a meat packaging company on December 2017 at A city in South Korea. Epidemiologic investigations were performed to clarify the cause and the transmission route of the outbreak. Methods : We conducted a questionnaire survey among 25 persons who worked at the company between July 1 and December 11, 2017, and we examined the 25 blood specimens and 36 environmental specimens for the C. burnetii. In addition, we examined on a farmer and 268 goat of the farm that supplied the meat, for the C. burnetii. The confirmed case was defined as a workers who participated in the company with positive results for the PCR or antibody test and responsible symptoms of Q fever. Results: The attack rate of the Q fever among the workers was 32%. The workers of meat packaging were found to be at the highest risk(relative risk 7.6; 95% confidence interval 1.187-52.951). Most of the meat packaging workers(75%) were not wearing masks during work, and there were no suitable ventilation and air conditioning facilities in the place where the meat packaging was done. In 10 of the 36 environmental specimens were positive PCR test for the C. burnetii (27.7%). 21 of 268 goats in the farm was infected by C. burnetii (7.8%), and the farmer was identified as asymptomatic case of Q fever. Conclusion: We concluded that the route of transmission of the outbreak was inhalation of aerosol generated during the cutting of goat meat infected by C. burnetii. Meat packaging workers need to wear masks and follow personal hygiene compliance, and the work area needs ventilation to ensure adequate air circulation. Infected patients must be required to follow-up to confirm chronic progression.