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RISS 인기검색어
- 검색키워드
- 주제어 : Carcinoembryonic antigen detection (검색결과 2 건)
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Ahn, Sujin,Yu, Hyunung,Kang, Seong Ho Elsevier 2017 Biosensors & bioelectronics Vol.96 No.-
<P><B>Abstract</B></P> <P>Carcinoembryonic antigen (CEA) is a glycoprotein associated with colorectal carcinomas and is commonly used as a clinical tumor marker. Enhanced detection sensitivity for the assay of CEA molecules was achieved on a plasmonic nanoimmunosensor by wavelength-dependent transmission grating (TG)-based total internal reflection scattering microscopy (TIRSM). The plasmonic nanoparticles were placed in an evanescent field layer on a glass nanoimmunosensor that produced evanescent wave scattering by the total internal reflection of light from two lasers. The light scattered by target protein (CEA)-bound 20-nm silver nanoparticles (plasmonic nanoprobes) was collected and spectrally isolated in first-order spectral images (<I>n</I>=+1) by a TG (70 grooves/mm). The combination of evanescent wave scattering and TG significantly enhanced the detection sensitivity and selectivity due to the minimized spectroscopic interference and background noise. The TG-TIRSM method detected the CEA molecules at concentrations down to 19.75zM with a wide linear dynamic range of 19.75zM-39.50nM (correlation coefficient, <I>R</I>=0.9903), which was 45 to 1.25×10<SUP>9</SUP> times lower than the detection limits and 2×10<SUP>5</SUP> to 2×10<SUP>11</SUP> times wider than the dynamic ranges of previous assay methods. In particular, by simply changing the antibody of the target molecule, this technique can be used to detect various disease-related protein biomarkers directly in human biological samples at the single-molecule level.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enhanced the detection sensitivity of CEA on nanoimmunosensor. </LI> <LI> Combined effects of wavelength-dependent TG-based TIRSM. </LI> <LI> Minimized the spectroscopic interference and background noise. </LI> <LI> 45 to 1.25×10<SUP>9</SUP> times lower than previous detection limits. </LI> <LI> 2×10<SUP>5</SUP> to 2×10<SUP>11</SUP> times wider dynamic range than previous methods. </LI> </UL> </P>
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Tran, Duy Thanh,Hoa, Van Hien,Tuan, Le Huu,Kim, Nam Hoon,Lee, Joong Hee Elsevier 2018 Biosensors & bioelectronics Vol.119 No.-
<P><B>Abstract</B></P> <P>A three-dimensional hierarchical nanohybrid based on Cu-Au bimetallic nanocrystals integrated carbon nanotube arrays vertically grown on carbon spheres was successfully developed as an active platform for sensing application. Such nanohybrid can provide abundant active sites and act as an exceptional platform for immobilizing highly dense and well-dispersed carcinoembryonic antibody (anti-CEA) to sensitively detect CEA, an emerging biomarker of various cancer diseases. Due to the unique nanoarchitecture with altered electronic structure of Cu-Au bimetallic catalyst and enhanced interactions between components, such nanohybrid based biosensor demonstrated excellent electrochemical performance towards CEA detection with great sensitivity, wide linear detection range (0.025–25 ng/mL), very low limit of detection (0.5 pg/mL), and good selectivity. The results imply that this sensor has great potential to offer essential information for cancer diagnosis and management with great clinical importance.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A 3D hierarchical Cu-Au nanocrystal integrated CNTs was grown on carbon sphere. </LI> <LI> The hybrid showed a wide linear detection range (0.025–25 ng/mL) and low detection limit (0.5 pg/mL). </LI> <LI> The hybrid showed good reproducibility and accurate detection of CEA in human serum. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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