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Shim, K.S.,Kim, S.E.,Yun, Y.P.,Jeon, D.I.,Kim, H.J.,Park, K.,Song, H.R. THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING 2017 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY -S Vol.55 No.-
<P>We fabricated biphasic calcium phosphate nanoparticles (BCP NPs)-immobilized on the surface of 3D printed PCL (BCP-IM-PCL) scaffolds, and evaluated in vitro osteogenesis and in vivo new bone formation in rat tibial defect model. In vitro and in vivo studies showed that BCP-IM-PCL significantly enhanced osteogenic markers (i.e., ALP activity, calcium deposition, and the expression of osteocalcin and osteopontin) and markedly increased new bone formation and mineralized bone tissues in tibial defect area, compared to unmodified PCL and BCP-mixed PCL scaffolds. This study demonstrated that BCP NPs-immobilized on the surface of PCL scaffolds are promising templates for bone tissue regeneration. (C) 2017 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.</P>
심규식,김성은,윤영필,Daniel I. Jeon,김학준,박경순,송해룡 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.55 No.-
We fabricated biphasic calcium phosphate nanoparticles (BCP NPs)-immobilized on the surface of 3D printed PCL (BCP-IM-PCL) scaffolds, and evaluated in vitro osteogenesis and in vivo new bone formation in rat tibial defect model. In vitro and in vivo studies showed that BCP-IM-PCL significantly enhanced osteogenic markers (i.e., ALP activity, calcium deposition, and the expression of osteocalcin and osteopontin) and markedly increased new bone formation and mineralized bone tissues in tibial defect area, compared to unmodified PCL and BCP-mixed PCL scaffolds. This study demonstrated that BCP NPsimmobilized on the surface of PCL scaffolds are promising templates for bone tissue regeneration.
Kim, Min-Sung,Kim, Yang-Hee,Park, Ih-Ho,Min, Young-Ki,Seo, Hyung-Seok,Lee, Byong-Taek Materials Research Society of Korea 2010 한국재료학회지 Vol.20 No.6
A highly porous Biphasic Calcium Phosphate (BCP) scaffold was fabricated by the sponge replica method with a microwave sintering technique. The BCP scaffold had interconnected pores ranging from $80\;{\mu}m$ to $1000\;{\mu}m$, which were similar to natural cancellous bone. To enhance the mechanical properties of the porous scaffold, infiltration of polycaprolactone (PCL) was employed. The microstructure of the BCP scaffold was optimized using various volume percentages of polymethylmethacrylate (PMMA) for the infiltration process. PCL successfully infiltrated into the hollow space of the strut formed after the removal of the polymer sponge throughout the degassing and high pressure steps. The microstructure and material properties of the BCP scaffold (i.e., pore size, morphology of infiltrated and coated PCL, compressive strength, and porosity) were evaluated. When a 30 vol% of PMMA was used, the PCL-BCP scaffold showed the highest compressive strength. The compressive strength values of the BCP and PCL-BCP scaffolds were approximately 1.3 and 2MPa, respectively. After the PCL infiltration process, the porosity of the PCL-BCP scaffold decreased slightly to 86%, whereas that of the BCP scaffold was 86%. The number of pores in the $10\;{\mu}m$ to $20\;{\mu}m$ rage, which represent the pore channel inside of the strut, significantly decreased. The in-vitro study confirmed that the PCL-infiltrated BCP scaffold showed comparable cell viability without any cytotoxic behavior.
Synthesis and characterization of biphasic calcium phosphate ceramics using a sponge coating method
이득용,김도현,김배연,박정윤,이준강 한양대학교 세라믹연구소 2018 Journal of Ceramic Processing Research Vol.19 No.1
Biphasic calcium phosphate (BCP) bone grafts were successfully synthesized using the 45 ppi polyurethane sponge coatingmethod. XRD results revealed that the BCP scaffolds were mainly composed of hydroxyapatite and β-tricalcium phosphate(β-TCP). As the number of BCP coatings increased from 1 to 5, the pore size and the wall size decreased from 480 ± 93 μmto 306 ± 120 μm and increased from 104 ± 25 μm to 186 ± 40 μm, respectively. The BCP scaffolds coated once, twice, threetimes, four times and five times, exhibited average cell viability of 106%, 109%, 114%, 107%, and 93%. The BCP scaffoldsshowed no evidence of causing cell lysis or toxicity. In addition, the cell proliferation results suggested that L-929 cells adheredwell to the BCP scaffolds and proliferated continuously with increasing time, indicating that the BCP powders are highlyapplicable to the synthetic bone grafts.
Effects of Collagen Grafting on Cell Behaviors in BCP Scaffold with Interconnected Pore Structure
양동준,전재희,이선영,안현욱,박근오,박광범,김석영 한국생체재료학회 2016 생체재료학회지 Vol.20 No.1
Background: This study was to investigate the effect of collagen grafted porous biphasic calcium phosphate (BCP) on cell attachment, proliferation, and differentiation. Porous BCP scaffolds with interconnected micropore structure were prepared with were prepared and then grafted with a collagen type I. The hydroxyapatite (HA) and β-tricalcium phosphate (TCP) ratio of the TCP scaffolds was about 60/40 and the collagen was crosslinked on the TCP scaffold surface (collagen-TCP). Results: The sintered BCP scaffolds showed fully interconnected micropore structures with submicron-sized grains. The collagen crosslinking in the scaffolds was conducted using the the N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (NHS) crosslinking method. The cell proliferation of collagen-BCP scaffolds showed a similar result to that of the BCP scaffolds. However, osteoblastic differentiation and cell attachment increased in the collagen-BCP scaffolds. Conclusions: Collagen-BCP scaffold improved the cell attachment ability in early phase and osteoblastic differentiation.