기름으로 오염된 토양에서 작물생육을 위한 계면활성제 생산 Bacteria의 활용에 관한 연구

황철원,장혜원,최용락,Hwang, Cher-Won,Chang, Hae-Won,Choe, Yong-Rak 한국생명과학회 2007 생명과학회지 Vol.17 No.7

Bacillus LPO3 (계면활성제와 같이 유화물질을 생산하는)을 hydrocarbon을 감소시키는 bio-control agent로 사용했다. 토양 (시중구입)은 gasoline을 섞어 오염시킨 토양을 사용하였다. 13일 동안 bacteria의 성장, hydrocarbon의 감소, 그리고, Bacillus LP03 의 작물 성장에 미친 요인(식물의 싹과 뿌리의 길이를 포함한)을 관찰하였다. 우리는 이 bacteria의hydrocarbon을 감소 시키는 물질로서 이 균이 생산하는 계면활성제일 것이라는 가능성을 이미 확인 하였으며 이는 식물의 싹과 뿌리의 생장을 촉진하였고, 회색 곰팡이에 대하여항 진균 활성을 보였다. 본 연구에서는 bacteria를 넣지 않은 기름으로 오염된 토양 실험 군에서는 식물이 자라나지 않았을 뿐 아니라 살아남지도 못했다. 실험 결과로 볼 때, gasoline의 hydrocarbon이 시간이 지남에 따라 감소되었고, RNA blotting실험에서는 오염된 토양에서 균의 증식과 더불어 계면활성제관련 유전자 산물의 증가를 확인하였다 결과적으로, 이 bacteria (계면활성제를 생산하는)는 회색 곰팡이에 감염된 토양이나 기름으로 오염된 토양에서 작물이 자랄 수 있게 하기 위한 bio-control agent로서 유효한 균으로 생각된다. Bacillus sp.LPO3 (producing emulsifying substances such as bio-surfactant) was used as a bio-control agent to degrade hydrocarbon (gasoline in oil spilled crop soil). The soil (brought from fertilizer store)was mixed with gasoline-spilled soil (made with Diatomaceous Earth, Sigma.U.S.A). The study was conducted for a period of 13 days, 13 days during which bacterial growth, hydrocarbon degradation and growth parameters of Bacillus sp.LP03 including shoot and root length were studied. We found that the effective of bacterial producing substance might bio-surfactants let the plants survive even more promote the growth of shoot and root length and showed antifungal activity against gray mold. Without the bacteria, they couldn't grow in oil-spilled soil not even survive. According to the results of the above experiments, we can see with following results, hydrocarbon in gasoline was reduced, day by day, then RNA dot blotting was done and it fit the results we had done. Finally, this Bacteria(producing bio-surfactant) were found to have effective bio-control agent for cropping in oil spilled soil and infected by gray mold.

Enhanced stability of bio-oil and diesel fuel emulsion using Span 80 and Tween 60 emulsifiers

Farooq, Abid,Shafaghat, Hoda,Jae, Jungho,Jung, Sang-Chul,Park, Young-Kwon Elsevier 2019 Journal of Environmental Management Vol.231 No.-

<P><B>Abstract</B></P> <P>Bio-oil (biomass pyrolysis oil) has some undesirable properties (e.g., low heating value, high corrosiveness, and high viscosity) that restrain its direct use as a transportation fuel. The emulsification of bio-oil and diesel is an effective and convenient method to use bio-oil in the present transportation fuel infrastructure. The addition of an emulsifying agent (emulsifier or surfactant) to two immiscible liquids of diesel and bio-oil is an important step in emulsification. The hydrophilic–lipophilic balance (HLB) value, according to the chemical structure and characteristics of the emulsifier, is a key parameter for selecting a surfactant. In this study, an ether treatment of raw bio-oil was carried out to separate the ether-soluble fraction of bio-oil from its heavy (dark brown and highly viscous) fraction, and the ether-extracted bio-oil (EEO) was processed further for emulsification into diesel fuel. The effects of the HLB value of the emulsifier and the contents of EEO, diesel, and emulsifier on the stability of the EEO/diesel emulsion were investigated. To optimize the HLB value of the emulsifier, different HLB values (4.3–8.8), which were prepared by mixing different amounts of Span 80 and Tween 60 as surfactants, were used for the EEO and diesel emulsification. A HLB value of 7.3 with diesel, EEO, and emulsifier contents of 90, 5, 5 wt%, and 86, 7.4, 6.6 wt% resulted in EEO/diesel emulsions (without phase separation) stable for 40 and 35 days, respectively. Measurement of the high heating value (HHV) of the emulsified fuels gave a 44.32 and 43.68 MJ/kg values for the EEO to emulsifier mass ratios of 5:5 and 7.4:6.6, respectively. The stability of emulsified EEO and diesel was verified by TGA and FT-IR methods.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Emulsification of ether-extracted bio-oil (EEO) in diesel was done at room temperature. </LI> <LI> Span 80 and Tween 60 in individual or combination form were used as emulsifiers. </LI> <LI> EEO/diesel emulsion was stable for 40 days, while no stratification was happened. </LI> <LI> Stability of EEO/diesel emulsion after 40 days was confirmed by TGA and FTIR. </LI> <LI> HHV of EEO/diesel emulsion was as high as 44 MJ/kg (near to diesel HHV of 45 MJ/kg). </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Effects of surfactants on the remediation of petroleum contaminated soil and surface hydrophobicity of petroleum hydrocarbon degrading flora

Jianbo Liu,Liming Xu,Feifei Zhu,Shouhao Jia 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.5

It has been proven that surfactants used in the remediation of petroleum hydrocarbon contaminated soil have great application potential. In this study, the effects of five surfactants (SDBS, Tween80, Tween60, rhamnolipid and TRS-1) on leaching of petroleum hydrocarbons from soil were investigated through orthogonal experiments, and petroleum hydrocarbon components were analyzed by GC/MS. The effects of surfactants on the degradation of petroleum hydrocarbon were analyzed by the changes of microbial growth curve and surface hydrophobicity. The results showed that surfactant type, temperature and surfactant concentration had significant effects on the removal rate of petroleum hydrocarbon. Tween80, rhamnolipid and TRS-1 have good bio-friendliness and a high removal rate of petroleum hydrocarbons (up to 65%), suitable for the restoration of the soil used in the experiment And Surfactants exhibited a higher removal rate for small molecules and petroleum hydrocarbons with odd carbon atoms. Surfactants have a certain modification effect on the surface of relatively hydrophilic bacteria under the initial conditions, making their surface properties develop in the direction of enhanced hydrophobicity, and the hydrophobicity has increased from less than 20% to about 40%.

Bio-surfactant mediated synthesis of Au/g-C3N4 plasmonic hybrid nanocomposite for enhanced photocatalytic reduction of mono-nitrophenols

Aparna Prabha Devi,Deepak Kumar Padhi,Pravat Manjari Mishra,Ajay Kumar Behera 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.108 No.-

A bio-surfactant assisted synthesis route has demonstrated in room temperature for decoration of goldnanoparticles (AuNPs) on g-C3N4 using Averrhoa carambola leaf extract towards mono-nitrophenol reductionunder visible light irradiation. Biomolecules of the leaf extract and optimised reaction conditionplayed a vital role on tuning the morphology and optoelectronic properties of AuNPs. The size ofAuNPs were found to be 15 ± 3 nm on 2D g-C3N4 sheets by TEM and STEM-HAADF analysis. Amongthe designed plasmonic hybrid photocatalysts 3-wt% loaded AuNPs on g-C3N4 showed 99.01% of 4-nitrophenol, 97.70% of 3-nitrophenol and 98.50% of 2-nitrophenol photoreduction. The photocatalyticefficiency of Au/g-C3N4 was investigated under several parameters such as pH, initial nitrophenol concentration,and in presence of external inorganic anions. The mechanism of photoreduction of nitrophenol toaminophenol by Au/g-C3N4 was proposed based on the intermediate obtained from GCMS analysis. Theobserved higher catalytic activity of plasmonic hybrid photcatalysts is due to the synergic chemistrybetween the conduction band of g-C3N4 and AuNP plasmonic band, which is well supported by PL, EIS,and transient photocurrent analysis. Kinetic rate constant for 3Au/g-C3N4 was found to be 0.43 min1at pH 3. Moreover, the rate of reaction significantly increased in presence of Cl. ICP-OES analysis of assynthesised 3Au/g-C3N4 plasmonic photocatalyst shows there was no gold leaching even after ten cyclesconfirming the stability and reusability of the catalyst.

Screening of bio-derived surfactants for soil washing of PAHs: effects of substrate sources and trace metals distribution

Nnanake-Abasi O. Offiong,Godwin J. Udo,Edu J. Inam,Akanimo N. Ekanem,Joachim J. Awaka-ama,Emaime J. Uwanta,Jun Dong 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.2

In this study, we screened the performance of aqueous extracts of Talinum triangulare (AET) and soap solution prepared from Hura crepitans seed oil saponified by aqueous solution of ashed plantain peels (HCS) for removal of naphthalene and phenanthrene from soil. The distribution of trace metals in the soil before and after soil washing was also investigated. The results revealed maximum removal efficiencies after 25 min washing time of 96.25, 96.14, and 25.70% naphthalene, for Tween 80, AET and HCS, respectively. While for phenanthrene, the recorded maximum removal efficiencies after 25 min washing time were: 91.80, 26.00, and 94.0 for Tween 80, AET and HCS, respectively. Based on results from other experiments, AET generally showed slightly lower removal efficiencies compared to the commercial Tween 80 surfactant. Also, the results revealed that the three remediants affected the distribution of trace metals (Cd, Pb, and Zn) in the soil after surfactant washing. Based on the amount of trace metals extracted, the performance of the three remediants are as follows: AET > Tween 80 > HCS. The performance of AET has been attributed to the presence of multiple heteroatomic moieties for trace metals adsorption and moderation of the acidic conditions of the soil.

Frog Foam Surfactant Protein for Emulsifier by its Low Surface Tension

Hye Jin HWANG,Seung Eun LEE,Ye Won LEE,Yun Jung YANG 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.4