Application of the Multiplex PCR Method for Discrimination of <i>Artemisia iwayomogi</i> from Other <i>Artemisia</i> Herbs

Lee, Mi Young,Doh, Eui Jeong,Kim, Eung Soo,Kim, Young Wha,Ko, Byong Seob,Oh, Seung-Eun Pharmaceutical Society of Japan 2008 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.31 No.4

<P>Some plants classified in the genus <I>Artemisia</I> are used for medicinal purposes. In particular, <I>A. iwayomogi</I>, which is referred to as ‘Haninjin,’ is used as an important medicinal material in traditional Korean medicine. However, <I>A. capillaris</I>, and both <I>A. argyi</I> and <I>A. princeps</I>, referred to as ‘Injinho’ and ‘Aeyup,’ respectively, are used for purposes other than those for which ‘Haninjin’ is utilized. However, it is occasionally difficult to differentiate ‘Haninjin’ from ‘Injinho’ and/or ‘Aeyup’ on the basis of their morphological features, particularly when in the dried and/or sliced form. Therefore, the development of a reliable method by which to discriminate ‘Haninjin’ from other <I>Artemisia</I> herbs, especially ‘Injinho’ and ‘Aeyup,’ is clearly necessary. We recently determined that the RAPD (random amplified polymorphic DNA) technique can be used to discriminate efficiently between some <I>Artemisia</I> herbs. In particular, when applied to RAPD, the non-specific UBC primer 391 (5′-GCG AAC CTC G-3′) was demonstrated to amplify PCR products specific to <I>A. iwayomogi.</I> Based on the nucleotide sequences of the PCR product, we designed a 2F1 (5′-ACC TCG GAC CTA AAT ACA-3′)/ 2F3 (5′-TTA TGA TTC ATG TTC AAT TC-3′) primer set to amplify a SCAR (sequence-characterized amplified region) marker of <I>A. iwayomogi</I>. Employing this primer set, along with two other primer sets amplifying SCAR markers of ‘Aeyup’ (<I>A. argyi</I> and <I>A. princeps</I>) and both ‘Injinho’ (<I>A. capillaris</I>) and <I>A. japonica</I>, which are classified into the same subgroup in a phenogram constructed from RAPD analysis, we developed a multiplex PCR method by which <I>A. iwayomogi</I> could be discriminated with certainty from other <I>Artemisia</I> herbs. <I>Via</I> this method, we determined not only whether the tested <I>Artemisia</I> herb was <I>A. iwayomogi</I>, but also which <I>Artemisia</I> herbs were tested concurrently with <I>A. iwayomogi</I>.</P>

실버세대 감성교육을 위한 천연염재 쑥속(Artemisia)식물의 염료가 염색의 기능성에 미치는 영향

이정은 ( Jung Eun Lee ),권영숙 ( Young Suk Kwon ) 한국환경과학회 2015 한국환경과학회지 Vol.24 No.5

The experience of natural dyeing is expected to be effective in promoting health through good exercise, in giving emotional stability based on the functional aromatic components of the dyeing material, and in improving self-esteem and increasing positive experiences through the behavior of creation. A natural dyeing material easily found around us, artemisia has long been used in food and Oriental medicine and was recently found to contain antioxidative, anticancer, deodorizing, antibacterial, anti-obesity, and anti-diabetes substances through chemical analysis. It has also been reported that group reminiscence therapy using the fragrance of artemisia is effective in relieving pain and depression and in promoting ego integrity in the elderly population. This study aimed to make a comparative analysis of dyeability, washing fastness, and deodorization between Artemisia princeps, Artemisia iwayomogi, and Artemisia annua, all of which are considered to be healthful and functional dyeing materials, among about thirty domestic plants in the genus of Artemisia and to provide basic data concerning natural dyeing in mind-peace education for the silver generation. The fabric dyed and post-mordanted at 80℃ for 40 minutes showed the greatest surface color variation; in this condition, artemisia princes (32.29) gave the greatest color difference, followed by artemisia iwayomogi (31.07) and artemisia annua (26.17). While all the types of artemisia were excellent in washing fastness, dry-cleaning fastness, and rubbing fastness at the fourth- or fifth-grade, light fastness was at the third grade for artemisia princes, at the second to third grade for artemisia annua, and at the second grade for artemisia iwayomogi; therefore, artemisia princes was found to give better fastness than the other two types of artemisia. In determining functionality of the fabric dyed with artemisia, deodorization test also found that the fabric dyed with artemisia princes had 20% higher deodorization.

Coumarins from the aerial parts of Artemisia iwayomogi Kitamura

( Trong Nguyen Nguyen ),( Hyeong-ju Jeon ),( Hyoung-geun Kim ),( Yeong-geun Lee ),( Seung Soo Lee ),( Myun Ho Bang ),( Nam-in Baek ) 한국응용생명화학회 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.4

Artemisia iwayomogi (Compositae), a perennial mugwort, is native to Korea and widely distributed in Japan, Russia, and China. A. iwayomogi and A. capillaris are similar in morphology and pharmacological activity and have been used for the same medicinal purposes in Korea. While various ingredients such as coumarins and flavonoids and their activity studies have been reported for A. capillaris, few studies have been conducted on the pharmacologically active components of A. iwayomogi. In Korea, A. capillaris is not economical because only young leaves are used as a medicinal material. Because of this, A. iwayomogi is frequently used in Korea, indicating the need to study its pharmacologically active components. Therefore, a phytochemical study was initiated to isolate active compounds from the aerial parts of A. iwayomogi. Finally, four coumarins, umbelliferone (1), esculetin (2), grevillone (3), and scoparone (4) were isolated for the first time from the aerial parts of A. iwayomogi in this study.

더위지기와 사철쑥의 정유성분 조성 비교

홍철운 ( Hong Cheol Un ) 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.1

The composition of essential oils isolated from the aerial parts of Artemisia iwayomogi Kitamura and Artemisia capillaris Thunberg collected from two diffenent cultivation area, respectively, was analyzed by GC and GC-MS. Sixty components were identified in oils from A. iwayomogi. The major components of A. iwayomogi oil collected from one area (Sample A) were iso-pinocamphone (31.64%), 1,8-cineole (21.55%), β-pinene (4.46%,), pinocarvone (3.72%), myrtenal (3.42%) and trans-pinocarvel (3.14%), and the major components of the oil from the other area (Sample B) were camphor (26.99%), 1,8-cineole (21.55%), α-terpineol (7.63%), borneol (4.10%), camphene (3.97%) and artemisia ketone (3.84%). Eighty components were identified in oils from A. capillaris. The major components were capillene (26.01~30.31%), β-pinene (8.55~18.38%), β-caryophyllene (8.80~13.70%). β-himachalene (1.67~5.57%), cis,trans-α-farnesene (2.10~7.25%) and germacrene D (2.27~5.46%) and there was no difference in oil composition of A. capillaris between two cultivation area.

한인진(韓茵蔯)의 생리활성에 관한 연구

송영은,류지성,정주리,곽준수,김대향,김범석,임채웅 한국약용작물학회 2001 한국약용작물학회지 Vol.9 No.2

한인진에 대한 항산화 효과와 항균 효과를 알아보기 위하여 추출용매별 고형분 함량을 측정하여 본 결과 물 추출물에서 18. 1%로 가장 많은 고형분을 얻을 수 있었으며 메탄올, 에탄을 추출물에서는 12. 8%, 12. 6%로 별 차이가 없었다. 항산화 효과를 알아보는 방법 중 DPPH 소거효과를 이용하여 측정하여 메탄올 추출물이 36.2μg/ml로 가장 효과 좋았지만, 대두유에 대한 한한인진 추출물의 항산화 효과를 60℃로 20일간 저장하면서 과산화물가(POV)를 측정하여 대두유를 기질로 한 저장시험 에서 1,000ppm 농도의 물추출물과 메탄올 추출물이 저장 기간이 20일 되었을 때 46.8(meq/kg, 50.8(meq/kg)의 과산화물가를 나타내 대조구의 과산화물가 79.1 (meq/kg)에 비해 낮았다. 식품의 부패균이나 식중독의 원인균으로 알려진 세균 4종을 대상으로한 항균실험에서는 추출 용매에 따른 항균력은 에탄올 추출물이 가장 좋았으며 세균에 대한 항균력으로는 Vibrio parahaemolyticus에 대해 가장 좋았다. 한인진의 간보호 효과 시험에서는 한인진 추출물을 경구투여한 V, IV군의 간무게, 체중에 대한 간무게의 비율이 생리 식염수만을 투여한 III군보다는 낮았으나 시료 채취전후로 한인진 추출물을 경구투여한 V군과 IV군사이에는 별다른 차이점이 없었다. 예방효과를 알아보기 위해 10일간 실험을 실시한 결과 한인진 물추출물만을 단독 투여한 VI군의 체중은 계속 증가하였으나 한인진 물추출물을 계속 투여한 다음 시료 채취전에 사염화탄소를 투여한 VIII군의 간무게, 체중에 대한 간비율이 대조군 VII보다 다소 감소하였다. 병리학적인 소견으로 한인진추출물을 투여 한 군에서는 사염화탄소만 투여한 군보다 간세포의 괴사와 지방적의 축적이 감소하여 사염화탄소에 의한 간독성에 효과가 있는 것으로 사료된다. This study was carried out to investigate antioxidative, antimicrobial activity and the effect on hepatotoxicity in various extracts of Artemisia iwayomogi. The herb has been used widely for jaundice, hepatitis and liver cirrhosis in chinese medicine. Solid yield by various extraction solvents, 18.1%, was the highest in water extract. To find antioxidative activity in Artemisia iwayomogi was estimated radical scavenging effect by DPPH method in various extracts and change of the POV(peroxide value) of various extracts added in soybean oil during 20 days at 60℃. Radical scavenging effect by DPPH method was the most effective in methanol extract. Added 1,000ppm water extract and methanol extract in soybean oil, the POV of them, 46.8(meq/kg) and 50.8(meq/kg) were lower than that of control, 79.1(meq/kg), during 20 days storage. After antimicrobial activity of various extracts of Artemisia iwayomogi on bacteria was carried out by paper disc method, it found that the ethanol extract was the strongest activity on Vibrio parahaemolyticus. In vivo experiment was to investigate the effect of Artemisia iwayomogi water extract(AIWE) on hepatotoxicity by carbon tetrachloride(CCl4) in rats. The experiment groups were divided into five groups for recovery(for 3 days) and three groups for protection(for 10 days) in rat liver. The weights and morphological changes of liver and the body weight were examined in each groups. Compared with CCl4 treatment groups(CCl4 only), liver and liver/body(%) weights of AIWE pretreatment groups for 3 days and AIWE posttreatment groups for 10 days were declined. In macrography, fibrious exudates and swelling of liver were decreased in AIWE treatment groups. Accumulation of lipid droplets and necrosis of hepatocytes were also decreased in AIWE treatment groups in microscopically. In these results, AIWE seems to enhance hepato-protective and recoverable effect on CCl4 induced hepatotoxicity in rats.

더위지기 생육 중 항산화 활성 변화

황태익 한국약용작물학회 2009 한국약용작물학회지 Vol.17 No.4

The aim of this study was to investigate the effect of plant growth at several different growing periods on antioxidant activities and zeatin and ABA contents of Artemisia iwayomogi. Measurements of antioxidant activities, lipid peroxidation inhibition, and superoxide radical scavenging activity were done using PMS, NBT and lipid auto-oxidation analysis, respectively. The results show that activities of antioxidants from Artemisia iwayomogi had much higher than BHT. DPPH free radical scavenging effect of Artemisia leaf extract was increased from 71.06±4.36% in April to 90.06±4.41% in October. Activities of superoxide radical scavenging and lipid peroxidation inhibition were 33.83±3.45% and 45.60±3.10 in April and then increased to 84.40±4.00% and 75.86±3.50% in October, respectively. An ELISA technique has been developed for the determination of zeatin and ABA in Artemisia leaves. By this method, the content changes of zeatin and ABA from Artemisia during the growth were investigated. The zeatin content in leaf was measured to be 186.86±1.40 pmol/g dry weight in April, however, decreased to 117.93±5.83 pmol in October. The ABA content in leaf increased from 19.00±1.26 nmol in April to 68.20±2.52 nmol in October. Relationship between antioxidant activities and plant hormone contents was indicated that antioxidant activity may depend on decreasing zeatin content or increasing ABA content.

Effect of Compositae Plants on Protein Levels in Streptozotocin-induced Diabetic Rats

Han, Hye-Kyoung,Kim, Gun-Hee Korean Society of Food Culture 2009 Food Quality and Culture Vol.3 No.1

The investigation assessed the influence of Compositae plants consumption on the protein profile in streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in Sprague-Dawley rats by injection of STZ (45 mg/kg body weight) into tail vein. The rats were randomly assigned to five groups: normal and STZ-control fed an AIN-93 diet, and groups whose diets were supplemented with 10% Compositae powder containing Artemisia iwayomogi (A. iwayomogi), Atractylodes lancea (A. lancea) or Taraxacum mongolicum (T. mongolicum). To observe the effects of Compositae plants in the animal model, the levels of protein in liver, kidney, lung, pancreas, and brain were determined after 4 weeks. The level of protein in kidney increased significantly in rats receiving the A. iwayomogi- and T. mongolicum-supplemented diet compared to the STZ-control group. The level of protein in lung was increased significantly in the A. iwayomogi-supplemented group. Blood glucose level correlated well with brain protein level but did not correlate with other protein levels. Also, blood glucose correlated inversely with kidney, lung and brain protein levels. It is suggested that supplementation with A. iwayomogi in diabetic rats leads elevates protein in kidney and lung.

Phenolic Compounds from <i>Artemisia iwayomogi</i> and Their Effects on Osteoblastic MC3T3-E1 Cells

Ding, Yan,Liang, Chun,Yang, Seo Young,Ra, Jeong Chan,Choi, Eun Mi,Kim, Jeong-Ah,Kim, Young Ho Pharmaceutical Society of Japan 2010 Biological & pharmaceutical bulletin Vol.33 No.8

<P>One new (4) and twelve known phenolic compounds (1—3, 5—13) were isolated from a 70% MeOH extract of the aerial parts of <I>Artemisia iwayomogi</I>. The new compound was identified as 7,8-dimethoxy-coumarin-9-<I>O</I>-(6′-<I>O</I>-(<I>E</I>)-coumaroyl)-β-<SMALL>D</SMALL>-glucopyranoside (4) and named iwayomin. The effects of compounds 1—13 on the function of osteoblastic MC3T3-E1 cells were examined by evaluating cell viability, alkaline phosphatase (ALP) activity, collagen synthesis, and mineralization in the presence of each compound. Compounds 3, 4, 7, and 9 showed potential in stimulating osteoblastic bone formation and may be useful for the prevention and/or treatment of osteoporosis.</P>

<i>Artemisia iwayomogi</i> Inhibits Immediate-Type Allergic Reaction and Inflammatory Cytokine Secretion

Shin, Tae-Yong,Park, Jeong-Suk,Kim, Sang-Hyun Taylor Francis 2006 IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY Vol.28 No.3

<P>The immediate-type allergic reaction is involved in many allergic diseases such as asthma and allergic rhinitis. The discovery of drugs for the treatment of immediate-type allergic diseases is a very important subject in human health. In this study, we investigated the effect of <I>Artemisia iwayomogi</I> (AIAE) on mast cell-mediated allergic reaction and inflammatory cytokine secretion. AIAE inhibited compound 48/80-induced systemic reactions in mice. AIAE decreased the passive cutaneous anaphylaxis (PCA) reaction activated by antidinitrophenyl (anti-DNP) IgE antibody. AIAE dose-dependently reduced histamine release from rat peritoneal mast cells activated by compound 48/80 or anti-DNP IgE. Furthermore, AIAE attenuated the phorbol 12‐myristate 13-acetate plus calcium ionophore A23187-stimulated tumor necrosis factor-α and interleukin-6 secretion in human mast cells. These results provide evidence that AIAE may be beneficial in the treatment of allergic diseases.</P>

Two New Phenolic Compounds from <i>Artemisia iwayomogi</i>

Yan, Xi‐,Tao,Ding, Yan,Li, Wei,Sun, Ya‐,Nan,Yang, Seo‐,Young,Koh, Young‐,Sang,Kim, Young‐,Ho WILEY‐VCH Verlag 2014 Helvetica chimica acta Vol.97 No.2

<P><B>Abstract</B></P><P>Two new phenolic compounds, (<I>Z</I>)‐5′‐hydroxyjasmone 5′‐<I>O</I>‐{6″‐<I>O</I>‐[(<I>E</I>)‐caffeoyl]‐<I>β</I>‐<SMALL>D</SMALL>‐glucopyranoside} (<B>1</B>) and quercetin‐7‐<I>O</I>‐<I>β</I>‐<SMALL>D</SMALL>‐glucuronide methyl ester (<B>2</B>), along with ten known phenolic compounds, <B>3</B>–<B>12</B>, were isolated from the aerial parts of <I>Artemisia iwayomogi.</I> Their structures were elucidated by spectroscopic methods, including 1D‐ and 2D‐NMR, and HR‐ESI‐TOF‐MS techniques. The inhibitory effects of compounds <B>1</B>–<B>12</B> on the LPS‐stimulated production of IL‐12 p40, IL‐6, and TNF‐<I>α</I> in bone marrow‐derived dendritic cells were evaluated.</P>