자외선B 조사 및 수종의 자외선차단제가 Hairless Albino Mouse의 피부노화에 미치는 영향

최재영 ( Jae Young Choi ),윤경식 ( Kyoung Sik Yoon ),조용호 ( Yong Ho Cho ),김낙인 ( Nack In Kim ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.2

Background: Photoaging skin is clinically characterized by wrinkled, dry, inelastic and irregularly pigmented skin, skin tumor and histologically by increased epidermal thickness, nurnerous fibroblasts, mast cells and inflammatory cells in the upper dermis, elastosis, degeneration of collagen fibers, increased proportion of type III collagen fibers in the dermis and increased numbers of keratinizing cysts in the lower dormis of hairless albino mouse skin. Chronic exposure to UVB induces photoaging skin and sunscreen agents are used to prevent photodamage to skin and reduce the incidence and extent of the chronic photoaging effects. Objective : To evaluate the photoaging effects of UVB on the skin and to assess the ability of sunscreen agents to protect the skin from photoaging, we examined the clinical, histological and quantitative changes in collagen in the skin of Albino hairless Skh: HR-1 mice. Materials and Methods : The experimental animals were male Albino hairless Skh: HR-1 mice, 12 weeks old. The control group was a chronologically aging group which had not been affected by UVB irradiation. The non-protected group was irradiated with UVB, a half of MED, 3 times weekly(Monday, Wednesday, Fr iday), for 12 weeks. To assess the photoprotective effects of sunscreen agents, the control group, the non-protected group and the sunscreen agent-applicated groups were compared to each other clinically, histologically, and by quantitative collagen analysis. An early increase in type III collagen during UVB irradiation was determined by cyanogen bromide digestion of the whole skin, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and densitometry. Results : Clinically, the skin of the mice in the non-protected group showed mild changes caused by photoaging. Histoloically, the non-protected group showed increases in the size and number of keratinizing cysts in the lower dermis and increases in the mast cells in the upper dermis compared with the control group. However, no significant findings of increased epidermal thickness, inflammatory cell infiltration, elastic fiber change or degeneration of collagen fibers were shown. By the 12th week, four of the total of nine mice in the non-protected group developed at least one tumor. The sunscreen agent applicated groups showed slightly photoprotected effects clinically and histologically. In the collagen analysis, the proportion of type III collagen was significantly increased in the photoaging skin of mice in the non-protected group compared with the control group. Mice in the sunscreen agent applicated groups showed a significantly decreased proportion of type III collagen compared with the non-protected group. Conclusion : To summarize, UVB exposure of the skin induces photoaging. The number and size of keratinizing cysts increased in the photoaging skin of hairless albino mice. There was also a quantitative change in the collagen fibers. The use of sunscreen agents decreases the photoaging effects of UVB on the skin. (Kor J Dermatol 1996;34(2): 289-299)

The soybean cultivar SCEL-1 shows potent anti-photoaging effects in a UV-induced three-dimensional human skin and hairless mouse model

Lee Jin Woo,Peng Lei,Jegal Hyun,Park No-June,Bong Sim-Kyu,Lee Joon Won,Pyo Jeong Joo,Choi Yongsoo,Kim Su-Nam 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.1

Soybean (Glycine max) is one of the major sources of nutrition and is used as a raw material for food and as a source of feed for livestock. The efficacy of soybeans on skin health includes their ability to reduce wrinkles and pigmentation and increase skin elasticity and moisture content. Black soybean has been consumed worldwide for a long time, especially in Korea, and is used as a medicinal food against several disorders related to the skin. To evaluate whether its effect on the skin is different based on the cultivar of soybeans, three black soybean cultivars collected in Korea, Soybean Core collection Elite Line-1 (SCEL-1), Chung Ja-3 (CJ-3) and Won Heuk (WH), were selected to compare their effect on improving photoaging induced by ultraviolet rays (UVs). We found that SCEL-1 exhibited the best efficacy among the three cultivars tested, and treatment with this soybean extract significantly reduced the expression of matrix metalloproteinase-1 (MMP-1), preventing the degradation of collagen in a 3D human skin model. In addition, SCEL-1 application improved wrinkle- and photoaging-related symptoms, such as epidermal thickening, collagen deficiency and immune cell infiltration, in an animal model established by UV irradiation. Procyanidin B2 and epicatechin isolated from the SCEL-1 cultivar inhibited MMP-1 biosynthesis in UVB-irradiated human dermal fibroblasts, and these two major components are likely related to more significantly attenuated skin photoaging. Therefore, our results indicated that SCEL-1 exhibits good anti-wrinkle effects compared to the other two black soybean cultivars, suggesting that it represents an excellent agent for anti-photoaging. Soybean ( Glycine max ) is one of the major sources of nutrition and is used as a raw material for food and as a source of feed for livestock. The efficacy of soybeans on skin health includes their ability to reduce wrinkles and pigmentation and increase skin elasticity and moisture content. Black soybean has been consumed worldwide for a long time, especially in Korea, and is used as a medicinal food against several disorders related to the skin. To evaluate whether its effect on the skin is different based on the cultivar of soybeans, three black soybean cultivars collected in Korea, Soybean Core collection Elite Line-1 (SCEL-1), Chung Ja-3 (CJ-3) and Won Heuk (WH), were selected to compare their effect on improving photoaging induced by ultraviolet rays (UVs). We found that SCEL-1 exhibited the best efficacy among the three cultivars tested, and treatment with this soybean extract significantly reduced the expression of matrix metalloproteinase-1 (MMP-1), preventing the degradation of collagen in a 3D human skin model. In addition, SCEL-1 application improved wrinkle- and photoaging-related symptoms, such as epidermal thickening, collagen deficiency and immune cell infiltration, in an animal model established by UV irradiation. Procyanidin B2 and epicatechin isolated from the SCEL-1 cultivar inhibited MMP-1 biosynthesis in UVB-irradiated human dermal fibroblasts, and these two major components are likely related to more significantly attenuated skin photoaging. Therefore, our results indicated that SCEL-1 exhibits good anti-wrinkle effects compared to the other two black soybean cultivars, suggesting that it represents an excellent agent for anti-photoaging.

Protection effect of donkey hide gelatin hydrolysates on UVB-induced photoaging of human skin fibroblasts

Kim, Jung-Soo,Kim, Dongwook,Kim, Hee-Jin,Jang, Aera Elsevier 2018 PROCESS BIOCHEMISTRY Vol.67 No.-

<P><B>Abstract</B></P> <P>UVB irradiation is a potent photoaging factor and leads to the formation of skin wrinkles. The objective of this study was to investigate the mechanisms of UVB photoaging inhibition of low molecular weight peptides derived from donkey hide gelatin hydrolysates (LDGH) on human skin fibroblasts. The donkey hide was hydrolyzed using proteases, and the LDGH exhibited higher antioxidant activity than the original hydrolysate and inhibited collagenase and elastase activities. We also found that LDGH increases viability of Hs68 cells after UVB irradiation at 100 mJ/cm<SUP>2</SUP>. Moreover, LDGH prevented the decrease in procollagen type I levels caused by exposure to UVB irradiation in Hs68 cells and reduced the up-regulated phosphorylation of p38, ERK, and JNK in the mitogen-activated protein kinase (MAPK) signaling pathway. These findings suggest that LDGH increases synthesis of procollagen type I by decreasing the phosphorylation of MAPK, therefore, LDGH may be useful as an effective anti-photoaging agent.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Short wavelength UV irradiation is a potent photoaging factor of the skin. </LI> <LI> Low size peptides from donkey gelatin hydrolysates (LDGH) inhibit UV photoaging. </LI> <LI> LDGH prevents UV irradiation-induced reduction in type I procollagen. </LI> <LI> LDGH prevents UV irradiation-induced activation of the MAPK signaling pathway. </LI> <LI> LDGH may be used as an effective anti-photoaging agent for the skin. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Anti-Photoaging Effect of Jeju Putgyul (Unripe Citrus) Extracts on Human Dermal Fibroblasts and Ultraviolet B-induced Hairless Mouse Skin

Choi, Seung-Hyun,Choi, Sun-Il,Jung, Tae-Dong,Cho, Bong-Yeon,Lee, Jin-Ha,Kim, Seung-Hyung,Yoon, Seon-A,Ham, Young-Min,Yoon, Weon-Jong,Cho, Ju-Hyun,Lee, Ok-Hawn MDPI AG 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.10

<P>Ultraviolet (UV) radiation stimulates the expression of matrix metalloproteinases (MMPs) and inflammatory cytokines. These signaling pathways participate in the degradation of the extracellular matrix and induce inflammatory responses that lead to photoaging. This study evaluated the antioxidant activity and the effect on MMPs and procollagen of putgyul extract in vitro. The anti-photoaging activity of putgyul extracts was estimated in vivo using hairless mice (HR-1). The putgyul extracts reduced MMP-1 production and increased the content of procollagen type I carboxy-terminal peptide in human dermal fibroblasts. Ultravilot-B (UVB)-induced expression of inflammatory cytokines and MMPs was detected in mice, and putgyul extracts suppressed the expression. These results suggest that putgyul extract inhibits photoaging by inhibiting the expression of MMPs that degrade collagen and inhibiting cytokines that induce inflammatory responses. The mouse model also demonstrated that oral administration of putgyul extracts decreased wrinkle depth, epidermal thickness, collagen degradation, and trans-epidermal water loss, and increased β-glucosidase activity on UVB exposed skin. Putgyul extract protects against UVB-induced damage of skin and could be valuable in the prevention of photoaging. </P>

항산화제의 광 노화 예방효과에 관한 분자적 기전 고찰

박지연,서영록 대한암예방학회 2008 Journal of cancer prevention Vol.13 No.3

Ultraviolet (UV)-induced aging has been reportedly accounted 80% of total aging process in human skin. Therefore, investigation on understanding the mechanism of UV-induced photoaging is essential for the prevention of skin aging. Mainly, the aging process induced by UV-generated ROS has been known to activate AP-1 factor, which stimulates several cellular pathways related with aging process including increase of inflammatory response, decrease of synthesis of dermal collagen I, III and inhibition for proliferation of keratinocyte. Recent studies have been suggested that several antioxidants, which play a role in remove ROS, have its own functions on prevention of photoaging. Furthermore, antioxidant has been reported to have potential role of increasing DNA repair activity, resulting in the removal of accumulated DNA damage. In this paper, molecular mechanisms in detail will be reviewed for the preventive effect of antioxidants on the protection against photoaging in human skin. (Cancer Prev Res 13, 162-168, 2008)

Protective Effects of Silkworm Hemolymph Extract and Its Fractions on UV-induced Photoaging

박주현,이지은,최신식,박태현 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.1

Ultraviolet (UV) irradiation induces skin photoaging by generating reactive oxygen species (ROS). ROS caused by UV-irradiation results in loss of skin cells and degradation of extracellular matrix. A number of antioxidants have been chemically synthesized or naturally extracted to prevent ROS-mediated skin photoaging. In our previous work, silkworm hemolymph extract (SHEX) was prepared, and its antioxidant activity was tested by free radical-scavenging assay. This study assessed the protective effects of SHEX on UV-induced photoaging of human immortalized keratinocytes (HaCaT). UVA (365 nm)-induced ROS generation was inhibited by supplementation of silkworm hemolymph (SH). Treatment with SHEX prepared by boiling SH inhibited death of HaCaT cells caused by UVB (315 nm) and UVA irradiation in a dose-dependent manner. Seven fractions were obtained by separating SHEX by gel permeation chromatography and the antioxidant activity of the fractions was examined. The fraction showing the highest protective efficacy on UV-induced cell damage corresponded to the lutein-containing fraction isolated in our previous study. Moreover, the SHEX fraction suppressed the expression of MMP-1 (matrix metalloproteinase-1), a matrix-degrading enzyme, suggesting that the active constituent of SHEX has the potential to inhibit skin photoaging. These results suggest that SHEX can be developed as a dietary and cosmetic supplement for prevention of skin photoaging.

Photothermal therapy using gold nanoparticle and 755-nm long-pulsed alexandrite laser for the improvement of photoaging: a pilot study

( Jin Seop Kim ),( Yeon Gu Choi ),( Gyoo Huh ),( Hyeon Jeong Park ),( Junghwa Yang ),( Yunho Lee ),( Heun Joo Lee ),( Won-serk Kim ),( Ga-young Lee ),( Young-jun Choi ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1

Background: Skin photoaging manifests deeper wrinkles and larger pore size. Photothermal therapy (PTT) using gold nanoparticle and long-pulsed laser has emerged as a new alternative for photoaging. Objectives: We aimed to determine the efficacy of PTT using gold nanoparticle and 755-nm long-pulsed alexandrite laser for photoaging. Methods: A prospective study of PTT using gold nanoparticle and 755-nm long-pulsed Alexandrite was designed. Three treatment sessions were performed at 4-week intervals. Clinical response was assessed 8, 12, and 20 weeks after the initial PTT session. Wrinkle and texture indices were evaluated by 3D skin analysis camera. Clinical improvement in wrinkles and pores assessed by two dermatologists using a 5-point quartile improvement scale (QIS) based on standardized facial medical photographs. Results: A total of 8 subjects (7 females and 1 male, mean age 64.8 ± 2.3 years) with the Glogau Photodamage Classification Scale III to IV was enrolled. The reduction of texture index was not significant, but the wrinkle index showed a significant improvement (p < 0.05). There was improvement of QIS in both wrinkle and pore (p < 0.05). Conclusion: PTT using gold nanoparticle and 755-nm long-pulsed alexandrite laser could be an effective treatment option for the improvement of photoaging.

The Methoxyflavonoid Isosakuranetin Suppresses UV-B-Induced Matrix Metalloproteinase-1 Expression and Collagen Degradation Relevant for Skin Photoaging

Jung, Hana,Lee, Eunjoo H.,Lee, Tae Hoon,Cho, Man-Ho MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.9

<P>Solar ultraviolet (UV) radiation is a main extrinsic factor for skin aging. Chronic exposure of the skin to UV radiation causes the induction of matrix metalloproteinases (MMPs), such as MMP-1, and consequently results in alterations of the extracellular matrix (ECM) and skin photoaging. Flavonoids are considered as potent anti-photoaging agents due to their UV-absorbing and antioxidant properties and inhibitory activity against UV-mediated MMP induction. To identify anti-photoaging agents, in the present study we examined the preventative effect of methoxyflavonoids, such as sakuranetin, isosakuranetin, homoeriodictyol, genkwanin, chrysoeriol and syringetin, on UV-B-induced skin photo-damage. Of the examined methoxyflavonoids, pretreatment with isosakuranetin strongly suppressed the UV-B-mediated induction of MMP-1 in human keratinocytes in a concentration-dependent manner. Isosakuranetin inhibited UV-B-induced phosphorylation of mitogen-activated protein kinase (MAPK) signaling components, ERK1/2, JNK1/2 and p38 proteins. This result suggests that the ERK1/2 kinase pathways likely contribute to the inhibitory effects of isosakuranetin on UV-induced MMP-1 production in human keratinocytes. Isosakuranetin also prevented UV-B-induced degradation of type-1 collagen in human dermal fibroblast cells. Taken together, our findings suggest that isosakuranetin has the potential for development as a protective agent for skin photoaging through the inhibition of UV-induced MMP-1 production and collagen degradation.</P>

Protective effect of porphyra-334 on UVA-induced photoaging in human skin fibroblasts

RYU, JINA,PARK, SU-JIN,KIM, IN-HYE,CHOI, YOUN HEE,NAM, TAEK-JEONG UNKNOWN 2014 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.34 No.3

<P>The significant increase in life expectancy is closely related to the growing interest in the impact of aging on the function and appearance of the skin. Skin aging is influenced by several factors, and solar ultraviolet (UV) irradiation is considered one of the most important causes of skin photoaging. The aim of this study was to examine the anti-photoaging role of porphyra-334 from <I>Porphyra (P.) yezoensis</I>, a mycosporine-like amino acid (MAA), using high-performance liquid chromatography (HPLC), and electrospray ionization-mass spectrometry (ESI-MS). In the present study, extracted UV-absorbing compounds from <I>P. yezoensis</I> included palythine, asterina-330 and porphyra-334. Porphyra-334 was the most abundant MAA in <I>P. yezoensis</I>, and it was therefore used for conducting antiphotoaging experiments. The effect of porphyra-334 on the prevention of photoaging was investigated by measuring reactive oxygen species (ROS) production and matrix metalloproteinase (MMP) levels, as well as extracellular matrix (ECM) components and protein expression in UVA-irradiated human skin fibroblasts. Porphyra-334 suppressed ROS production and the expression of MMPs following UVA irradiation, while increasing levels of ECM components, such as procollagen, type I collagen, elastin. These results suggest that porphyra-334 has various applications in cosmetics and toiletries because of its anti-photoaging activities and may serve as a novel anti-aging agent.</P>

Oral Administration of Lactobacillus plantarum HY7714 Protects Hairless Mouse Against Ultraviolet B-Induced Photoaging

( Hyun Mee Kim ),( Dong Eun Lee ),( Soo Dong Park ),( Yong Tae Kim ),( Yu Jin Kim ),( Ji Woong Jeong ),( Sung Sik Jang ),( Young Tae Ahn ),( Jae Hun Sim ),( Chul Sung Huh ),( Dae Kyun Chung ),( Jung H 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11

Ultraviolet (UV) irradiation alters multiple molecular pathways in the skin, thereby inducing skin damage, including photoaging. In recent years, probiotics have gained interest due to their beneficial effects on skin health, such as inhibiting atopic dermatitis and improving skin immunity or inflammation. However, little is known about the effects of probiotics on UVBinduced photoaging. In this study, we evaluated the effect of Lactobacillus plantarum HY7714 against UVB-induced photoaging in human dermal fibroblasts and hairless mice. The results showed that L. plantarum HY7714 treatment effectively rescued UVB-reduced procollagen expression through the inhibition of UVB-induced matrix metalloproteinase (MMP)-1 expression in human dermal fibroblasts. Data from a western blot showed that L. plantarum HY7714 inhibited the phosphorylation of Jun N-terminal kinase, thereby suppressing the UVB-induced phosphorylation and expression of c-Jun. Oral administration of L. plantarum HY7714 clearly inhibited the number, depth, and area of wrinkles in hairless mouse skin. Histological data showed that L. plantarum HY7714 significantly inhibited UVB-induced epidermal thickness in mice. Western blot and zymography data also revealed that L. plantarum HY7714 effectively inhibited MMP-13 expression as well as MMP-2 and -9 activities in dermal tissue. Collectively, these results provide further insight regarding the skin biological actions of L. plantarum HY7714, a potential skin anti-photoaging agent.