Hematological characteristics and endocrine profiles of cloned dromedary camels (Camelus dromedaries)

Young-Bum Son,Mohammad Shamim Hossein,Yeon Ik Jeong,Mina Kang,Huijeong Kim,Yura Bae,Kung Ik Hwang,Alex Tinson,Singh Rajesh,Al Shamsi Noura,Woo Suk Hwang The Korean Society of Animal Reproduction and Biot 2024 한국동물생명공학회지 Vol.39 No.1

Background: Somatic cell nuclear transfer (SCNT) is a prominent technology that can preserve superior genetic traits of animals and expand the population in a short time. Hematological characters and endocrine profiles are important elements that demonstrate the stability of the physiological state of cloned animals. To date, several studies regarding cloned camels with superior genes have been conducted. However, detailed hemato-physiological assessments to prove that cloned camels are physiologically normal are limited. In this study, We evaluated the hemato-physiological characteristics of cloned male and female dromedary camels (Camelus dromedaries). Methods: Therefore, we analyzed variations in hematological characteristics and endocrine profiles between cloned and non-cloned age-matched male and female dromedary camels (Camelus dromedaries). Two groups each of male and female cloned and non-cloned camels were monitored to investigate the differences in hemato-physiological characteristics. Results: All the animals were evaluated by performing complete blood count (CBC), serum chemistry, and endocrinological tests. We found no significant difference between the cloned and non-cloned camels. Furthermore, the blood chemistry and endocrine profiles in male and female camels before maturity were similar. Conclusions: These results suggest that cloned and non-cloned camels have similar hematological characteristics and endocrine parameters.

Hematological characteristics and endocrine profiles of cloned dromedary camels (Camelus dromedaries)

손영범,Hossein Mohammad Shamim,정연익,강미나,김희정,배유라,황경익,Tinson Alex,Rajesh Singh,Noura Al Shamsi,황우석 사단법인 한국동물생명공학회 2024 Journal of Animal Reproduction and Biotechnology Vol.39 No.1

Background: Somatic cell nuclear transfer (SCNT) is a prominent technology that can preserve superior genetic traits of animals and expand the population in a short time. Hematological characters and endocrine profiles are important elements that demonstrate the stability of the physiological state of cloned animals. To date, several studies regarding cloned camels with superior genes have been conducted. However, detailed hemato-physiological assessments to prove that cloned camels are physiologically normal are limited. In this study, We evaluated the hemato-physiological characteristics of cloned male and female dromedary camels (Camelus dromedaries). Methods: Therefore, we analyzed variations in hematological characteristics and endocrine profiles between cloned and non-cloned age-matched male and female dromedary camels (Camelus dromedaries). Two groups each of male and female cloned and non-cloned camels were monitored to investigate the differences in hemato-physiological characteristics. Results: All the animals were evaluated by performing complete blood count (CBC), serum chemistry, and endocrinological tests. We found no significant difference between the cloned and non-cloned camels. Furthermore, the blood chemistry and endocrine profiles in male and female camels before maturity were similar. Conclusions: These results suggest that cloned and non-cloned camels have similar hematological characteristics and endocrine parameters.

멸종위기의 백한우(체세포복제 포함)의 성장 및 혈액학적 특성 분석

김현,최창용,성환후 사단법인 한국동물생명공학회 2015 한국동물생명공학회지 Vol.30 No.3

The objective of this study was to monitor health conditions of genetically identical somatic cells cloned Korean white cattle, endangered indigenous cattle (EIC) and indigenous cattle (IC) by analysis of hematologic characteristics. Naturally ovulated oocytes and donor cells were used for somatic cell nuclear transfer (SCNT). Donor cells and enucleated oocytes were followed by electric fusion, chemical activation and surgical embryo transfer into the oviducts of surrogate females. Two recipients became pregnant; two maintained pregnancy to term, and one live cattle were delivered by caesarean section. The cloned Korean white cattle were genetically identical to the nuclear donor cattle. As a result, the mean values of RBC and platelet of cloned cattle and white cattle were significantly decreased by age (P<0.05). The mean values of RBC, HCT, MCV and MCHC between cloned cattle and IC of the same age (1∼2 years) showed the statistical significance (P<0.05). Also, in the WBC of Korean white cattle, the estimated values were decreased according to the age from 12.0×103/μl under 1 year to 11.0×103/μl over 1 years respectively. Although clone-cattle had lower numbers of RBC than reference range, the most of RBC and WBC related heamatologic results of cloned cattle were not different when compared to reference range. This study suggests that cloned Korean white cattle derived from SCNT did not have remarkable health problems, at least in the growth pattern and hematological parameters. In addition, this study provides a valuable resource for further investigations of the preservation of rare genetic stocks underlying traits of interest in cattle.

체세포 복제 자견의 성장 및 혈액학적 특성 분석

김동훈,최미경,노진구,박종주,염동현,김현민,최봉환,김동교,박진기,류재규 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.3

The objective of this study was to monitor health conditions of four genetically identical somatic cells cloned Labrador retriever puppies by estimation of body weight and analysis of hematologic and serologic characteristics. Naturally ovulated oocytes and donor cells were used for somatic cell nuclear transfer (SCNT). Donor cells and enucleated oocytes were followed by electric fusion, chemical activation and surgical embryo transfer into the oviducts of surrogate females. Two recipients became pregnant; two maintained pregnancy to term, and four live puppies were delivered by Caesarean section. The cloned Labrador retrievers were genetically identical to the nuclear donor dog. The body weight of clone-1, -2, -3, and -4 was increased from 0.66, 0.40, 0.39, and 0.37 kg at birth to 6.2, 6.6, 6.2, and 6.0 kg at 8 weeks of age, respectively. Although clone-4 had lower numbers of RBC than reference range, the most of RBC and WBC related heamatologic results of cloned puppies were not different when compared to reference range. In serological analysis, Glucose, ALP and inorganic phosphate level of four cloned puppies was significantly higher than the reference ranges. However, there was no significant difference among four cloned dogs. This study suggests that cloned puppies derived from SCNT did not have remarkable health problems, at least in the growth pattern and hematological and serological parameters.

체세포 복제한우에서 장기적인 혈액화학적 특성 분석

양병철,이휘철,정학재,김경운,안진희,정희경,김동훈,변승준,임기순,박진기 사단법인 한국동물생명공학회 2011 Reproductive & developmental biology Vol.35 No.3

The purpose of the production of somatic cell cloned animals through cloning excellent cattle meat and milk production. However, at birth or after birth in cloned animal health problems such as the large offspring syndrome,malformations and sudden death has already been reported. Successfully overcome the initial problems of cloning animals for growing that there are other issues will need to continue to monitor. The objective of this study was to determine the long term health status for the cloned bovine and their offspring. Whole blood collected from 83 months (9 cow) female clone, 72 months (1) male clone and their offspring and hematological analysis was performed. To collect basic data about the safety of cloned animals and their offspring the RBC, WBC, cytokine were scanned. As a result, the red blood cells, HCT, Hb, MCV, MCH, MCHC levels between cloned animals and the controls did not differ significantly. White blood cells, platelet, S. neutro, lymphocyte, monocyte, eosinophil levels, protein, albumin,Bilirubin, γ-GT, K, amylase, creatinine, glucose, calcium, Mg, insulin-like growth factor 1(IGF-1), TNF-a, IL-6, IL-10levels between clone and control cow are no different significantly. The immune globulin and potassium levels showed differences, however, the difference between normal and within reference range. These results suggested that health status of cloned animal and their funder is not something to worry about until now. Although, between clone and control was no difference in hematological characteristics, these and other factors, but also the long-term research will be needed.

동합금 가두리 망 사육어류, 조피볼락(Sebastes schlegeli)의혈액 성상과 중금속 축적률

양성진 ( Sung Jin Yang ),전제천 ( Je Cheon Jun ),박정준 ( Jung Jun Park ),명정인 ( Jeong In Myeong ),신윤경 ( Yun Kyung Shin ) 한국어류학회 2014 韓國魚類學會誌 Vol.26 No.3

동합금 가두리망에 6개월간 사육한 조피볼락을 대상으로동합금 가두리 망에서 유출되는 물질이 어류의 생존, 성장및 건강도에 미치는 영향을 조사하였다. 대조구와 실험구간의 생존율과 성장률 그리고 혈액 성상은 유의한 차이가 없었으며(P/0.05), 간세포의 형태 및 간 조직 내에 존재하는 모세혈관과 담관의 구조가 특이적인 차이를 보이지 않았다. 구리와 아연 축적량은 실험 초기에 비해 증가하였지만 실험구와 대조구 간의 차이가 없었으며 허용기준치에 비해 매우 낮게 나타나 본 연구에 사용된 동합금망은 어류의 생존, 성장 및 건강도에 영향을 끼치지 않는 것으로 여겨진다. The effect of substances discharged from copper alloy mesh on the survival rate, growth, and health status of Sebastes schlegeli was investigated. Survival rate of experimental group was 10%higher than control group. There was no significant difference in weight gain and SGR between control group and experiment group (P/0.05). Glucose concentration was lower in the experimental group than that in the control group. GOT and GPT contents did not show significant difference during experiment except for the early three months of experiment (P/0.05). Ammonia concentration had not significantly changed in the experimental group, but it had increased until four months of experiment and then decreased afterwards in the control group. TCHO had decreased in the experiment group compared with that of control group. Copper and zinc contents had increased as compared with those in the initialst age of experiment with no significant difference between experiment group and control group(P/0.05). Histological analysis for the liver revealed that liver tissues were not particularly different from those in control group. There were no significant differences in survival rate, growth, and hematological characteristic between control group and experiment group (P/0.05). Though copper and zinc were accumulated as compared with those during the initial stage of experiment, the levels were lower than permissible levels for copper and zinc. As a result, copper alloy mesh would not adversely affect on the survival rate, growth, and health status of fishes.

EDCs 오염 하천수에 노출된 붕어, Carassius auratus의 생존율과 혈액학적 변화

정애진(Ae Jin Jung),김재원(Jae Won Kim),주선미(Sun Mi Ju),이정식(Jung Sick Lee) 전남대학교 수산과학연구소 2012 수산과학연구소논문집 Vol.20 No.-

본 연구는 붕어, Carassius auratus의 생존율과 혈액학적 성상에 미치는 EDCs combination effect를 알아보기 위하여 수행하였다. EDCs-polluted water는 낙동강 구미 공업단지 하류에서 채수하였다. 실험구는 대조구와 3개 (50, 75 및 100%)의 EDCs-polluted water였으며, 실험기간은 16주였다. 생존율은 75%와 100% 농도구에서 대조구에 비해 유의적으로 감소하였다 (P<0.05). 혈액학적 성상은 대조구에 비해 100% 농도구에서 GOT, GPT, LDH 농도는 유의적으로 증가하였다 (P<0.05). 본 연구 결과, EDCs-polluted water는 붕어의 생존율 감소와 혈액학적 성상의 변화를 유도함이 확인되었다. This study aims to find out the EDCs (endocrine disrupting chemicals) combination effect on survival rate and hematological characteristics of the crucian carp, Carassius auratus. EDCs-polluted water was collected from downstream of the Gumi electric industrial complex of the Nackdong River. The experimental groups were composed of one control condition and three EDCs-polluted water conditions (50, 75 and 100%). The experimental period was 16 weeks. The survival rates of 75% and 100% exposure group were significantly decreased relative to the control (P<0.05). The concentrations of GOT, GPT and LDH in 100% exposure group were significantly increased relative to the control (P<0.05). The results of this study suggest that EDCs-polluted water induces reduction of survival rate and change of hematological characteristics in the crucian carp.

EDCs 오염 하천수에 노출된 붕어, Carassius auratus의 생존율과 혈액학적 변화

정애진 외 전남대학교 수산과학연구소 2012 수산과학연구소논문집 Vol.20 No.-

This study aims to find out the EDCs (endocrine disrupting chemicals) combination effect on survival rate and hematological characteristics of the crucian carp, Carassius auratus. EDCs-polluted water was collected from downstream of the Gumi electric industrial complex of the Nackdong River. The experimental groups were composed of one control condition and three EDCs-polluted water conditions (50, 75 and 100%). The experimental period was 16 weeks. The survival rates of 75% and 100% exposure group were significantly decreased relative to the control (P<0.05). The concentrations of GOT, GPT and LDH in 100% exposure group were significantly increased relative to the control (P<0.05). The results of this study suggest that EDCs-polluted water induces reduction of survival rate and change of hematological characteristics in the crucian carp.

Effect of DEHP Administration on Reproductive Characteristic and Blood Metabolite in Mice

박동헌,장현용,박춘근,정희태,김정익,양부근 한국발생생물학회 2004 발생과 생식 Vol.8 No.2

본 연구는 내분비 장애물질 중 관찰물질로 지정된 저 농도의 DEHP의 투여가 생쥐의 번식기능에 미치는 영향을 검토하였으며 다음과 같은 결과를 얻었다. 웅성 생쥐에 DEHP를 체중 kg당 무처리구, 대조구(corn oil 투여구), 0.5, 1.0 및 10.0mg DEHP를 투여한 구에서 실험개시시와 실험종료시의 무게(정소상체, 정낭선 및 응고선)는 투여구 간에 커다란 차이가 없었으나, 정소의 무게는 DEHP 투여구가 대조구에 비해 다소 높은 경향을 나타 The purpose of this experiment was to determine the effects of di(2-ethylhexyl) phthalate(DEHP) on reproductive characteristic, blood hematological and chemical values in mice. The male mice were intraperitoneally injected DEHP in negative control(no treatment), positive control(corn oil, 3ml/kg B.W), 0.5, 1.0 and 10.0mg DEHP/kg B.W and the female mice were injected DEHP in control(corn oil, 3ml/kg B.W), 0.5, 1.0 and 10.0mg DEHP/kg B.W with 5 times for 15 days on 3 days interval. The administration of DEHP in male mice were not affect on body weight, epididymis, vesicular gland and coagulating gland weight. The testis weight were slightly higher in DEHP treatment groups than in control. The semen characteristics(sperm concentration, viability, motility and abnormality) of male mice were not difference in all experimental groups. The RBC, Hb, HT, MCV, MCH, MCHC< PLT, albumin, BUN and total protein of blood hematological and chemical values were not affect the administration of DEHP in mice. The WBC values in 10.0mg DEHP group was slightly difference in all experimental group(P>0.05). The histological evaluation of testis, ovary and affevt the reproductive characteristic, blood hematological and chemical values.

Effects of the Vibration Stress on Cortisol and Hematological Characteristics in Soft-shelled Turtle, Pelodiscus sinensis

허준욱,이정열,장영진,배승철,박인석 한국발생생물학회 2009 발생과 생식 Vol.13 No.1

We examined the effects of the vibration stress on cortisol secretion and hematological characteristics in soft-shelled turtle, Pelodiscus sinensis. For the stressed group vibration of from electric vibrator applied for 30 min with 2-h intervals during daytime () up to 28 days. Using the blood samples collected from ten turtles held once a week after vibration stress, we measured hematocrit, hemoglobin, red blood cells, cortisol, glucose, lactic acid, osmolality, , aspartate aminotransferase (AST), and alanine aminotransferase (ALT). The results have showed that P. sinensis received vibration stress exhibit the 'typical' stress-induced physiological responses (cortisol, glucose, lactic acid, osmolality, ions, hematocrit and hemoglobin) induced by vibration stress. Our data suggested that chronic vibration stress caused substantial stress in the animal, and in particular, the persisting elevated levels of AST and ALT would be highly correlated with the adverse effects of the stress. The high hematological characteristics during entire experimental period showed that the P. sinensis could not adapt to chronic stimuli provoked by vibration stress.