한국 남해안에 출현하는 태평양참다랑어 Pacific bluefin tuna, Thunnus orientalis의 식성

윤상철 ( Sang Chul Yoon ),유준택 ( Joon Taek Yoo ),이성일 ( Sung Il Lee ),김장근 ( Zang Geun Kim ),최광호 ( Kwang Ho Choi ) 한국어업기술학회 2015 수산해양기술연구 Vol.51 No.4

The feeding habits of the Pacific bluefin tuna, Thunnus orientalis were examined based on the stomach contents of 330 specimens caught by offshore large purse seine fishery in the southern sea of Korea, 2011. The size of Pacific bluefin tuna used in this study ranged from 34.1 to 67.3 cm in fork length (FL). The percentage of empty stomachs was 41.8%. The main prey items were Pisces and Cephalopoda based on percentage IRI (index of relative importance). The main fish preys were Trachurus japonicus, Bregmaceros japonicus, Engraulis japonicus, and Cephalopoda were Todarodes pacificus, Loligo edulis, Watasenia scintillans. T. orientalis showed ontogenetic change in diet composition. Although Pisces dominated the diet of all length classes the portion of Cephalopoda was relatively higher in size between 40 and 50 cm other than length classes. In terms of seasonal variation in feeding habits, Pisces was the main prey group in all seasons, but Cephalopoda was also frequently consumed during spring and autumn based on %IRI.

참다랑어 Thunnus orientalis의 종묘생산과 치어의 적정 사육수조 및 단백원 검토

지승철(Seung-Cheol Ji),타카오카 오사무(Osamu Takaoka),타키이 켄지(Kenji Takii),정관식(Gwan-Sik Jeong),한석중(Seock-Jung Han) 한국수산과학회 양식분과 2008 韓國養殖學會誌 Vol.21 No.4

The relationship between fishing characteristics of Pacific bluefin tuna (Thunnus orientalis) and ocean conditions around Jeju Island

신아리,윤상철,이성일,박희원,김수암 한국수산과학회 2018 Fisheries and Aquatic Sciences Vol.21 No.1

Pacific bluefin tuna (Thunnus orientalis) is one of the commercially important species in Korea as well as other countries of the North Pacific. Korean offshore large purse seine fisheries targeting small pelagic fishes such as chub mackerel have caught T. orientalis temporarily in the east of Jeju Island. The catch of T. orientalis in March through June occupied approximately 60% of the total. The monthly catch around Jeju Island from 2004 to 2013 showed a negative correlation (r = − 0.755, p < 0.01) with the seawater temperature at 50 m and had a significant positive correlation (r = 0.856, p < 0.01) with the Pacific Decadal Oscillation Index (PDOI). The highest catch and catch per unit effort (CPUE) of T. orientalis around Jeju Island occurred either when the seawater temperature ranged between 15 and 16 °C at 50 m or when the catch was taken near the frontal area where two water masses from offshore and coastal areas collide. The length of T. orientalis caught around Jeju Island from 2004 to 2013 ranged from 19 to 193 cm in fork length (FL). The time series of the monthly mean FL of T. orientalis had a negative correlation (r = − 0.592, p < 0.01) with the seawater temperature at 50 m and had a significant positive correlation (r = 0.668, p < 0.05) with PDOI.

The relationship between fishing characteristics of Pacific bluefin tuna (Thunnus orientalis) and ocean conditions around Jeju Island

Shin, Ari,Yoon, Sang Chul,Lee, Sung Il,Park, Hee Won,Kim, Suam The Korean Society of Fisheries and Aquatic Scienc 2018 Fisheries and Aquatic Sciences Vol.21 No.1

Pacific bluefin tuna (Thunnus orientalis) is one of the commercially important species in Korea as well as other countries of the North Pacific. Korean offshore large purse seine fisheries targeting small pelagic fishes such as chub mackerel have caught T. orientalis temporarily in the east of Jeju Island. The catch of T. orientalis in March through June occupied approximately 60% of the total. The monthly catch around Jeju Island from 2004 to 2013 showed a negative correlation (r = - 0.755, p < 0.01) with the seawater temperature at 50 m and had a significant positive correlation (r = 0.856, p < 0.01) with the Pacific Decadal Oscillation Index (PDOI). The highest catch and catch per unit effort (CPUE) of T. orientalis around Jeju Island occurred either when the seawater temperature ranged between 15 and $16^{\circ}C$ at 50 m or when the catch was taken near the frontal area where two water masses from offshore and coastal areas collide. The length of T. orientalis caught around Jeju Island from 2004 to 2013 ranged from 19 to 193 cm in fork length (FL). The time series of the monthly mean FL of T. orientalis had a negative correlation (r = - 0.592, p < 0.01) with the seawater temperature at 50 m and had a significant positive correlation (r = 0.668, p < 0.05) with PDOI.

참다랑어(Thunnus orientalis) 치어에 있어서 전갱이(Trachrus japonicus) 근육 엑기스 중의 섭이촉진물질 검색

지승철,타카오카오사무,세오카마나부,코오바라준,호소카와히테츠요,시메노사다오,정관식,이시우,타키이켄지,Ji, Seung-Cheol,Takaoka, Osamu,Seoka, Manabu,Kohbara, Jun,Hosokawa, Hidetuyo,Shimeno, Sadao,Jeong, Gwan-Sik,Lee, Si-Woo,Takii, Kenji 한국양식학회 2007 韓國養殖學會誌 Vol.20 No.1

참다랑어(Thunnus orientalis)의 배합사료 개발을 위해 전갱이(Trachrus japonicus) 근육 합성 엑기스(SE)를 사용하여 omission test를 통해 섭이촉진물질을 검색하였다. 인공종묘 생산된 참다랑어 치어(평균체중 $9.0{\pm}0.91\;g$: 실험 1, 2, 3; $1.6{\pm}0.23\;g$: 실험 4)를 사용하여 전갱이 근육 100 g 상당 함량의 각각의 섭이촉진 물질을 카제인 기초 사료에 첨가하여 총 4회의 사육실험을 실시하였다. 실험 1에서 인공합성엑기스(SE)와 SE로부터 아미노산 관련 화합물(SE-A), 핵산관련물질(SE-N) 그리고 유기염기화합물(SE-O)을 각각 제외한 시험액을 제조하여 천연 전갱이 근육 엑기스(NE)와 비교한 결과, SE-A와 SE-N구는 SE보다 낮은 섭이활성을 보였으나, SE-O구는 SE와 유사한 섭이활성을 보였다. 실험 2에서는 IMP가 섭이 촉진에 가장 많은 영향을 미치는 것이 인정되었으며, 실험 3에서는 L-alanine, L-glutamic acid, L-histidine, L-lysine, taurine 그리고 IMP의 혼합물이 SE와 섭이활성의 차이를 보이지 않았다. 실험 4에서는 L-histidine, L-glutamine 그리고 IMP 혼합물이 가장 높은 섭이활성을 보였으며, 그 다음으로는 SE, L-lysine, L-alanine and IMP 혼합물, L-histidine, L-glutamic acid, L-lysine and L-alanine과 IMP의 혼합물이 높은 섭이활성을 보였다. 본 연구결과 참다랑어 치어의 유효 섭이촉진물질은 L-histidine, L-glutamic acid 그리고 IMP로 판단되었다. For establishing a basal diet for the Pacific bluefin tuna Thunnus orientalis (PBT), feeding stimulants were initially identified by omission test using the synthetic extract of horse mackerel, Trachurus japonicus. Four feeding trials were conducted using juvenile PBT weighing $9.0{\pm}0.91\;g$ (trial 1, 2 and 3) and $1.6{\pm}0.23\;g$ (trial 4), which were originated from an artificial seedling production. The fish fed the casein diet with each test solution were added at the ratio of 100 g casein diet to 100 g jack mackerel muscle. A complete synthetic extract of jack mackerel containing all 3 fractions, amino acid, nucleotide and organic nitrogenous base, exhibited a comparable feeding stimulant activity compared to that of natural extract. The omission of nucleotide or amino acid fraction showed lower feeding activity, but the omission of other nitrogenous fraction maintained a similar feeding stimulant activity compared to that of the synthetic extract (trial 1). Inosine-5' monophosphate $Na_2$ (IMP) was identified as a major constituent for maintaining feeding activity. The mixture of L-alanine, L-glutamic acid, L-histidine, L-lysine, taurine and IMP induced a similar feeding activity compared to that of the synthetic extract (trial 2 and 3). In trial 4, the highest feeding activity was finally obtained in the mixture of L-histidine, L-glutamine and IMP, followed by the synthetic extract, the mixture of L-lysine, L-alanine and IMP, IMP and the mixture of L-histidine, L-glutamic acid, L-lysine and L-alanine. These results revealed that the mixture of L-histidine, L-glutamic acid and IMP for the proper feeding stimulant of PBT in this study.

한국 연근해에 출현하는 태평양참다랑어 Pacific bluefin tuna, Thunnus orientalis 의 식성

조헌주 ( Heon Ju Jo ),이성일 ( Sung Il Lee ),김두남 ( Doo Nam Kim ),이미경 ( Mi Kyung Lee ) 한국수산해양기술학회(구 한국어업기술학회) 2019 수산해양기술연구 Vol.55 No.1

The feeding habits of Pacific bluefin tuna, Thunnus orientalis were analyzed using samples by large purse seine fishery in Korean waters from 2012 to 2017. The number of samples was 1,274 and the fork-length (FL) ranged from 34.6 to 218.0 cm. According to %IRI, the Important main prey items of immature individuals (below 91.4 cm in FL) were Pisces, Cephalopoda and Euphausiacea and those of mature individuals were Cephalopoda and Pisces. T. orientalis showed ontogenetic changes in prey item that Pisces was dominated in size class of 30-89 cm (FL), Cephalopoda in 90-179 cm (FL), and Pisces above 180 cm. As for seasonal changes in prey item, Cephalopoda was dominant in spring and Pisces was dominant in summer, autumn and winter. %F, %N, %W and %IRI cluster analysis divided area into three groups: Group A was dominated by Pisces; Group B was dominated by Cephalopoda, and Group C was dominated by Euphausiacea.

한국 연근해에 출현하는 태평양참다랑어 Pacific bluefin tuna, Thunnus orientalis 의 식성

조헌주,이성일,김두남,이미경 한국수산해양기술학회 2019 수산해양기술연구 Vol.55 No.1

The feeding habits of Pacific bluefin tuna, Thunnus orientalis were analyzed using samples by large purse seine fishery in Korean waters from 2012 to 2017. The number of samples was 1,274 and the fork-length (FL) ranged from 34.6 to 218.0 cm. According to %IRI, the Important main prey items of immature individuals (below 91.4 cm in FL) were Pisces, Cephalopoda and Euphausiacea and those of mature individuals were Cephalopoda and Pisces. T. orientalis showed ontogenetic changes in prey item that Pisces was dominated in size class of 30-89 cm (FL), Cephalopoda in 90-179 cm (FL), and Pisces above 180 cm. As for seasonal changes in prey item, Cephalopoda was dominant in spring and Pisces was dominant in summer, autumn and winter. %F, %N, %W and %IRI cluster analysis divided area into three groups: Group A was dominated by Pisces; Group B was dominated by Cephalopoda, and Group C was dominated by Euphausiacea. 한국 연근해에 출현하는 태평양참다랑어의 식성을 분석하기 위해 2012년 부터 2017년 까지 대형선망어업에서 어획된 샘플을 분석하였다. 샘플은 총 1,274개체이고 가랑이체장(FL)의 범위는 34.6~218.0cm였다. 미성숙개체(91.4cm이하) 의 먹이생물중요도는 어류, 두족류, 난바다곤쟁이순이었으며 성숙개체는 두족류, 어류 순이었다. 체장크기군별 먹이생물 중요도는 30~89cm 크기군에서는 어류가 우점하였고, 90~179cm 크기군에서는 두족류가 우점하였고, 180cm 이상 개체에서는 어류가 우점하였다. 계절별 먹이생물 중요도는 봄철에는 두족류가 우점하였고, 여름, 가을, 겨울철은 어류가 우점하였다. 해역별 클러스터 분석을 실시한 결과 어류가 우점하는 지역, 두족류가 우점하는 지역, 난바다곤쟁이류가 우점하는 지역으로 나눌수 있었다.

Genome Wide Computational Identification of Tuna (Thunnus orientalis) MicroRNAs and Their Targets

Sangita Chowdhury Paul,Ashutosh Sharma,Richa Mehta,Sujay Paul 한국해양과학기술원 2018 Ocean science journal Vol.53 No.4

Applying genome-wide computational-based approaches (using the draft genome sequence published in recent years) and following a set of strict filtering criteria, a total of 48 potentially conserved microRNAs belonging to 30 families were identified from economically important fish tuna (Thunnus orientalis). Using BLAST and RNA hybrid program a total of 19 potential miRNA targets were also identified in this study, which includes a number of signaling molecules (serine/threonine-protein kinase, GTPase activating protein etc.) and transcription factors (F-box protein, zinc finger protein etc.). This study provides the basis for gaining a better understanding of miRNA-mediated gene regulatory processes in fishes.