Proteus vulgaris and Proteus mirabilis Decrease Candida albicans Biofilm Formation by Suppressing Morphological Transition to Its Hyphal Form

이경호,박주영,박수정,최선주 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.6

Purpose: Candida albicans (C. albicans) and Proteus species are causative agents in a variety of opportunistic nosocomial infections,and their ability to form biofilms is known to be a virulence factor. In this study, the influence of co-cultivation with Proteus vulgaris (P. vulgaris) and Proteus mirabilis (P. mirabilis) on C. albicans biofilm formation and its underlying mechanisms were examined. Materials and Methods: XTT reduction assays were adopted to measure biofilm formation, and viable colony counts were performedto quantify yeast growth. Real-time reverse transcriptase polymerase chain reaction was used to evaluate the expression of yeast-specific genes (rhd1 and rbe1), filament formation inhibiting genes (tup1 and nrg1), and hyphae-related genes (als3, ece1, hwp1, and sap5). Results: Candida biofilm formation was markedly inhibited by treatment with either living or heat-killed P. vulgaris and P. mirabilis. Proteus-cultured supernatant also inhibited Candida biofilm formation. Likewise, treatment with live P. vulgaris or P. mirabilisor with Proteus-cultured supernatant decreased expression of hyphae-related C. albicans genes, while the expression of yeast-specific genes and the filament formation inhibiting genes of C. albicans were increased. Heat-killed P. vulgaris and P. mirabilistreatment, however, did not affect the expression of C. albicans morphology-related genes. Conclusion: These results suggest that secretory products from P. vulgaris and P. mirabilis regulate the expression of genes related to morphologic changes in C. albicans such that transition from the yeast form to the hyphal form can be inhibited.

光州川에서 分離한 Proteus屬의 生化學的性狀 및 抗菌劑 感受性에 관한 硏究

朴悅 順天鄕大學校 1980 의대논문집 Vol.5 No.-

Using the law of Edwards-Ewing and Minitek System, it will be shown that Proteus Enterobacteria was detected in Kwangju River. When the biochemical characteristics and antibiotic susceptibility were tested for the 32 Proteus strains identified in the Enterobacteria in which lactose non-fermentation and an oxidase non-reactive character were discovered. Thc following results were obtained. 1. The isolated strains were as follows : P. vulgaris : 3 strains. P. mirabilis : 3 strains. P. morganii : 1 strain. P. rettgeri : 25 strains. 2. Biotype IV (14 strains) was found to be the most abundant type among the P. rettgeri. 3. The isolated strains were almost as predicted by the law of Edward-Ewing. The strains were individually isolated on performing the tests of H_(2)S, Ornithine, Urea, and Inositol on the 25 P. rettgeri. The results of the Indol reactions revealed 4(16.%) negative strains, 6(24%) Rhamnose negative strains, and 3 negative strains of Phenylalanlne and one nesative strain of Citrate. 4. All 2 strains were found to be susceptible to Gentamyoin and Nalidixic Acid. Three(9.4%) strains were found to be resistance to Sulfamethoxazole and Streptomycin ; seven(22%) to Cabenicillin ; 9(36%) to Chlorampheniccol ; twenty-two(69%) to Ampicillin ; twenty-four(75%) to Cephalothin ; twenty-six(81%) to Colistin ; twenty-nine(91%) to Erythromycm ; thirteen(94%) to Tetracycline, and all 32 to Penicillin. The average rate of resistance was found to be 48.2 percent. 5. Multi-resistant strains were distributed as follows : Four-multi-resistant strains : 2 strains Five-multi-resistant strains : 13 strains Six-multi-resistant strains : 10 strains Seven multi-resistant strains : 5 strains Eight-multi-resistant strains ' 2 strains

배양액의 ATP첨가 및 이온 농도에 따른 Amoeba proteus 수축포의 배출작용

최범선,주윤수안태인 한국통합생물학회 1991 동물학회지 Vol.34 No.4

담수산 대형 아메바인 각moeba proteus의 위상차 현미경 관찰 및 사진 분석을 통하여 수축포의 배출활동을 조사하f:다. Chalkley's 무기 염류 배양액에 첨가한 0. 1 mM ATP(disodium salt)에 의해 수축포의 배출속도는 270%로 증가하f:으며, 이 ATP의 효과는 Na+ 이온농도가 0.46mM 이상일 때 유효하였다. 실험용액의 NaGl 농도를 10 mM까지 증가시켰을 때 배출작용은 230%에 이르기까지 완만한 직선적 증가를 보였으며, 0.1 mM ATP를 첨가했을 때는 소폭의 NaCl농도 증가(0.50 mM)에 대하여 급격한 상승을 보였다. 이 배출 촉진은 Na+이온에 대해서 선별적으로 이루어졌으며 K+이온으로는 대체될 수 없었다. 배출속도는 Cac12를 제외한 Chalkley's 액에 50 $\mu$ M EDTA(disodium)를 첨가하였을 때에는 2900ye로 증가하였으며 , Caclf 농도가 증가됨에 따라 현격한 감소를 보였다. Chalkley's용액의 Cac12, NaCl을 함께 제외한 경우 배출속도는 대조군 수준에 미달된 데 비하여 0.2 mM Cac12, 10 mM NaCl첨가시에는 대조군의 180%였다. 아메바 수축포의 배출작용이 Na+이온 배출기구로 보고(Pottier efaf., 1987) 이들 결과를 종합해 볼때 아메바의 세포막에는 Na+ 이온의 투과수단으로 칼슘제거에 의해서 촉진확산되는 것과 Na+이온 농도증가에 따른 단순확산이 있을 것으로 사료된다.

아메바 세포막에 대한 단항체 생산 및 이를 이용한 막 조성 물질의 역할규명

안태인,최지영 한국통합생물학회 1989 동물학회지 Vol.32 No.4

Monoclonal antibodies (MAbs) reacting with the plasmalemma of Amoeba proteus were produced. Specificity of the 3 MAbs was determined by transfer blotting of the SDS polvacryfamide gel. AMS antibody reacted with the mucopolysaccharide bands of the spacer gel, 220 KD and 50 KD proteins of the resolving gel. The maior glycoprotein bands (175 KD, 165 KD) and 50 KD protein of the plasmalemma were recognized by AUG antibody. A third, AMP antibody reacted with the 50 KD protein only. In immunofluorescence microscopy of the enzyme treated cells, the antigens of these MAbs were sensitive to proteases, but not sensitive to neuraminidase. In the assay of cell to substratum attachment after binding with the antibody, AMG and AMP antibodies exerted no effect, but AMS hindered the attachment and cell spreading. Thus the effective components of the plasmalemma in cell to substratum attachment appear to be the mucopolysaccharides and 220 KD protein. The membranes of latex particle infested phagosomes did not show any distinction from the plasmalemma in fluorescence microscopy. Phagosome membranes of amoebae appear to be derived from the plasma membrane without selection in terms of the antigen composition. Amoeba Proteus의 세포막과 반응하는 단세포군 항체를 생산하였다. SDS polyacrylamide gel을 transfer blotting하여 이들 항체의 반응 특이성을 조사해 본 결과 AMS 단항체는 PAS로 염색되는 spacer gel의 mucopolysaccharide 린드, resolving gel의 220 KD 및 50 KD 단백질과 반응하였으며, 세포막의 주요 당단백질인 175 KD 및 165 KD 빈드와 50 KD 단백질은 AMG 단항체에 의해서 인지되었다. 그리고 AMP단항체는 공통인 50 KD 단백질과 특이하게 반응하였다. 효소처리한 아메바의 면역형광칠미경적 조사에서 이들 항체에 대한 항원분자들은 모두 단백질분해효소에 민감하였으며 neuraminidase에 대해서는 변화가 없었다. 이들 항체를 결합시킨 아메바의 용기표면 부착 가능성을 분석한 결과 AMP 및 AMG 단항체는 아무런 영향을 미치지 못하였으며 AMS 단항체는 세포의 용기표면 부착 및 세포의 펴짐을 저해하였다. 따라서 아메바의 용기표면 부착은 mucopolysaccharide 및 220 KD 단백질에 의해서 매게되는 것으로 나타났다. 그리고 latex particle을 담고 있는 식포막은 면역형광형미경적 조사에서 세포막과 차이가 없었다. 따라서 겐포막은 항원 조성에 있어서 비 선택적으로 세포막에서 유도되는 것으로 나타났다.

Proteus의 가상 통신 포트 활용에 관한 연구

한상배(Sang-Bae Han),김남호(Nam-Ho Kim) 한국정보통신학회 2023 한국정보통신학회 종합학술대회 논문집 Vol.27 No.1

청상방풍탕의 Proteus sp.에 대한 항균효과에 관한 연구

이은숙 ( Eun Sook Lee ),서부일 ( Bu Il Seo ) 대한본초학회 2004 大韓本草學會誌 Vol.19 No.2

N/A Objectives : The purpose of this research is to verify antibiotic effects of Cheongsangbangpungtang on Proteus sp. Antimicrobial activities were assayed through the hot water extracts from Cheongsangbangpungtang. The author screened and isolated a Proteus sp. from acne vulgaris patients. Methods : This study measured an antimicrobial activities of Cheongsangbangpungtang through agar diffusion method and verified MIC on Proteus sp. This study also investigated optimization of pH and temperature through growth curve of Proteus sp. cultivated and examined stabilities of Cheongsangbangpungtang on temperature and pH. Results : The optimum pH and temperature for the growth of the organism were in the range 7.0 and 37℃, respectively. Bangpung that is more than size 10mm of inhibition zone among Cheongsangbangpungtang composition drugstuffs. Cheongsangbangpungtang showed inhibition zone of 9.54㎜. Minimal Inhibitory Concentrations(MIC) of Cheongsangbangpungtang composition drugstuffs extract is 3-10㎕. Conclusion : Antimicrobial activity of the Cheongsangbangpungtang extract was not destroyed by the heating at 121℃ for 15min and not affected by pH. The water extract of Cheongsangbangpungtang composition drugstuffs was found to have a strong inhibition activity on the growth of Proteus sp.

프로테우스 증후군 -증례 보고

윤석주,유지연,이현숙,박영옥 大韓再活醫學會 1997 Annals of Rehabilitation Medicine Vol.21 No.6

악골의 편측 비대를 보이는 프로테우스 증후군의 치료: 증례보고

윤영은,설가영,오민석,최정구,김상중,이동근,강지연,Yun, Yeong-Eun,Seol, Ka-Young,Oh, Min-Seok,Choi, Jung-Goo,Kim, Sang-Jung,Lee, Dong-Keun,Kang, Ji-Yeon 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.6

Proteus syndrome is a congenital hamartomatous malformation that is characterized by a wide range of deformities, including craniofacial deformities. Proteus syndrome features partial gigantism and asymmetry of the limbs, plantar hyperplasia, hemangiomas, lipomas, lymphangiomas, varicosities, verrucous epidermal nevi, macrocephaly, cranial hyperostosis, and long bone overgrowth. We diagnosed Proteus syndrome in a male patient who visited our hospital with a chief complaint of limited mouth opening and report the case because we obtained a good healing outcome after treating the condition with a corrective osteotomy.

Proteus 증후군의 증례보고 : Report of First Korean Case

한승규 최신의학사 1995 最新醫學 Vol.38 No.4

Proteus Syndrome: Report of a Case with Developmental Glaucoma

Zuleyha Sik Sarman,Nursen Yuksel,Hakan Sarman,Dilek Bayramgurle 대한안과학회 2014 Korean Journal of Ophthalmology Vol.28 No.3

The purpose of this study was to report developmental glaucoma and pseudopapilledema in a patientwith Proteus syndrome. We defined the presence of developmental glaucoma, right pseudopapilledemaand myopia in a 4.5-year-old patient with Proteus syndrome. Marked right hemihypertrophy, lipoma,macrodactyly, and asymmetry of the limbs were observed on systemic examination. A cavernoma wasalso detected in magnetic resonance imaging of the brain. The patient underwent bilateral goniotomysurgery due to glaucoma. The surgical outcomes were satisfactory in both eyes. In conclusionsdevelopmental glaucoma and pseudopapilledema might be associated with Proteus syndrome.