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신경연 ( Gyeong Yeon Shin ),최혜정 ( Hye Jung Choi ),강양래 ( Yang-rae Kang ),남기두 ( Ki-du Nam ),송주영 ( Ju Yeong Song ),주우홍 ( Woo Hong Joo ) 한국미생물생명공학회 2017 한국미생물·생명공학회지 Vol.45 No.2
For the production of bioethanol by the synchronous saccharification and fermentation (SSF) process, bio-capsule formation was attempted. Many saccharifying fungal strains and fermentative yeast strains were first screened. Aspergillus sp. BCNU 6200, Penicillium sp. BCNU 6201, and P. chrysogenum KACC 44363 were found to be excellent producers of saccharifying enzymes such as α-amylase and glucoamylase. Saccharomyces cerevisiae IFO-M-07 showed the highest ethanol productivity among the tested strains. Secondly, we determined the optimal conditions for pellet formation, and those for bio-capsule formation. All the tested fungal strains formed pellets, and the optimal conditions for bio-capsule formation were 28℃ and 120 rpm. Lastly, SSF process was performed using a bio-capsule. An ethanol yield of 3.9% was achieved by using the Aspergillus sp. BCNU 6200 bio-capsule (Aspergillus sp. BCNU 6200 + S. cerevisiae IFO-M-07) at 30℃ with shaking at 120 rpm during the 10 days of incubation. The results provide useful information on the application of a bio-capsule in bioethanol production under the SSF process.
한우 거세비육우에 반추위 강화 사료첨가제 급여가 바이오캡슐 생체정보 및 비육능력에 미치는 영향
양가영,김한솔,김대현,이준구,오동엽,박진연,하재정 한국산학기술학회 2022 한국산학기술학회논문지 Vol.23 No.8
The objective of this study was to determine the effects of dietary probiotics and pH modifier supplementation on growth (GP) performance, carcass traits (CT), and ruminal biometric sensors in Hanwoo steers. A total of 32 Hanwoo steers equipped with a bio-capsule in the rumen were recruited (18.5 months). A total of 4 dietary treatments including control C (without supplementation), T1 (probiotics; 150 g/d), T2 (pH modifier; 100 g/d), and T3 (pH modifier; 200 g/d) were tested. The GP, CT, ruminal biometrics (pH, temperature, and activity), and economic analysis were obtained during a 10-month study period. The average daily gain and feed-conversion-ratio were greater (P < 0.05) with the T2 and T3 supplementation than that in C and T1. The hierarchy for the ruminal pH and activity was: C < T3 < T2 < T1 (P < 0.05). Carcass grades, including carcass weight, longissimus muscle area, marbling score, and firmness in the steers fed T2 and T3 diets were greater (P < 0.05) than those fed C and T1 diets. The net profit based on the economic analyses was 0.5, 1.3, 2.0, and 2.5 million won/head for the C, T1, T2, and T3 diets, respectively. The supplementation of additives could thus partially prevent a decrease in pH and provide relatively healthy activities. 본 연구는 한우 비육우 전용 반추위 강화 사료첨가제 개발의 일환으로 체류형 바이오캡슙내 pH, 반추위 체온 및 활동성 센서를 활용한 생체정보 수집으로 개체별 건강성을 고려한 비육능력 개선의 효과를 확인하고자 수행하였다. 시험방법으로는 18.5개월령의 한우 거세우 32두를 공시하여 대조구(무첨가), 처리1구(생균제 150g/일), 처리2구(반추위 강화 첨가제 100g/일), 처리3구(반추위강화첨가제 200g/일) 총 4처리로 10개월간 급여하면서 사양성적, 생체정보, 도축성적을 수집하고 경제성 분석을 실시하였다. 수집된 데이터의 통계처리는 SAS의 MIXED procedure를 활용하였다. 연구결과 일당증체량에서는 무첨가구와 생균제 처리구 0.7kg 대비 반추위 강화 첨가제 급여구에서 0.8kg으로 증가하였고, 사료요구율에서도 유의적(p<0.05)으로 동일한 결과를 나타냈다. 생체정보 관련으로 모든 항목(반추위 pH, 체온, 활동성)은 C < T3 < T2 < T1 순으로 낮은 결과를 보였다(p<0.05). 도축성적으로는 무첨가구 대비 반추위 강화 첨가제 급여구에서 도체중(CW), 등심단면적(LMA), 근내지방도(MS), 조직감(FI) 항목에서 유의적(p<0.05)으로 우수한 품질로 판정되었다. 추가적으로 경제성 분석에 따른 순이익으로는 두당 무첨가 대조구 50여만원, 생균제 급여구 130여만원, 반추위 강화 첨가제 급여구 200~250여만원 순으로 분석되었다. 따라서, 본 연구결과를 바탕으로 한우 비육우의 반추위 강화 첨가제 제공은 pH 급격한 감소 방지와 함께 비교적 건강한 활동을 제공할 수 있으며, 농가소득 증대를 위한 기초자료로 활용될 것으로 판단된다.
Differences in ruminal temperature between pregnant and non-pregnant Korean cattle
Kim, Dae Hyun,Ha, Jae Jung,Yi, Jun Koo,Kim, Byung Ki,Kwon, Woo-Sung,Ye, Bong-Hae,Kim, Seung Ho,Lee, Yoonseok The Korean Society of Animal Reproduction and Biot 2021 한국동물생명공학회지 Vol.36 No.1
In recent years, various methods of measuring body temperature have been developed using wireless biosensors to facilitate an early detection of pregnancy and parturition in cows. However, there are no studies on real-time monitoring of cattle body temperature throughout pregnancy. Therefore, we investigated the daily mean ruminal temperature in pregnant cows throughout pregnancy using a ruminal bio-capsule sensor and then evaluated the temperature variation between pregnant and non-pregnant cows. In pregnant cows, the mean and standard deviation of ruminal temperature was 38.86 ± 0.17℃. Ruminal temperature in pregnant cows slowly decreased until 180 to 190 days after artificial insemination and after that, the temperature increased dramatically until just before parturition. Furthermore, the means ruminal temperature was significantly different between pregnant and nonpregnant cows. The mean and standard deviation of ruminal temperature were as follows: 38.68 ± 0.01℃ from days 80 to 100, 38.78 ± 0.02℃ from days 145 to 165, 38.99 ± 0.45℃ from days 200 to 220, 39.14 ± 0.38℃ from days 250 to 270 before parturition. Therefore, our results could provide useful data for early detection of pregnancy and parturition in Korean cows.
Differences in ruminal temperature between pregnant and non-pregnant Korean cattle
Dae Hyun Kim,Jae Jung Ha,Jun Koo Yi,Byung Ki Kim,Woo-Sung Kwon,Bong-Hae Ye,Seung Ho Kim,Yoonseok Lee 한국동물생명공학회(구 한국동물번식학회) 2021 한국동물생명공학회지 Vol.36 No.1
In recent years, various methods of measuring body temperature have been developed using wireless biosensors to facilitate an early detection of pregnancy and parturition in cows. However, there are no studies on real-time monitoring of cattle body temperature throughout pregnancy. Therefore, we investigated the daily mean ruminal temperature in pregnant cows throughout pregnancy using a ruminal bio-capsule sensor and then evaluated the temperature variation between pregnant and non-pregnant cows. In pregnant cows, the mean and standard deviation of ruminal temperature was 38.86 ± 0.17℃. Ruminal temperature in pregnant cows slowly decreased until 180 to 190 days after artificial insemination and after that, the temperature increased dramatically until just before parturition. Furthermore, the means ruminal temperature was significantly different between pregnant and nonpregnant cows. The mean and standard deviation of ruminal temperature were as follows: 38.68 ± 0.01℃ from days 80 to 100, 38.78 ± 0.02℃ from days 145 to 165, 38.99 ± 0.45℃ from days 200 to 220, 39.14 ± 0.38℃ from days 250 to 270 before parturition. Therefore, our results could provide useful data for early detection of pregnancy and parturition in Korean cows.