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빙핵활성단백질의 N-terminal부분을 이용한 녹색형광단백질의 Zymomonasmobilis세포 표면 발현
이은모(Lee,Eun-Mo ),최신건(Choi,Shin-Geon) 강원대학교 산업기술연구소 2009 産業技術硏究 Vol.28 No.B
Green fluorescent protein (GFPuv) was displayed on the surface of ethanol-producing bacteria Zymomonas mobilis using N-terminaldomain of ice nucleation protein (INP) as an anchoring motif. To evaluate the ice nucleation protein as plausible anchor motif in Z. mobilis, GFPuv gene was subcloned into Zymomonas expression vector yielding pBBR1MCS-3/pPDC/INPN/GFPuv plasmid., INP-GFPuv fusion protein was expressed in Z.mobilis and its fluorescence was verified by confocal microscopy. The successful display of GFPuv on Zymomonas mobili ssuggest that INP anchor motif could be used for future fusion partner in Z. mobili sstrain improvement.
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빙핵활성단백질의 N-terminal 부분을 이용한 녹색형광단백질의 Zymomonas mobilis 세포 표면 발현
이은모,최신건 江原大學校 産業技術硏究所 2009 産業技術硏究 Vol.29 No.B
Green fluorescent protein (GFPuv) was displayed on the surface of ethanol-producing bacteria Zymomonas mobilis using N-terminal domain of ice nucleation protein (INP) as an anchoring motif. To evaluate the ice nucleation protein as plausible anchor motif in Z. mobilis, GFPuv gene was subcloned into Zymomonas expression vector yielding pBBR1MCS-3/pPDC/INPN/GFPuv plasmid., INP-GFPuv fusion protein was expressed in Z. mobilis and its fluorescence was verified by confocal microscopy. The successful display of GFPuv on Zymomonas mobilis suggest that INP anchor motif could be used for future fusion partner in Z. mobilis strain improvement.
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장예진,지미란,전미향,김점순,김경운,한덕우,정학재,양병철,류재규,박진기,김태완,변승준 韓國家禽學會 2012 韓國家禽學會誌 Vol.39 No.3
본 연구는 형질전환 닭에서 주입한 외래 유전자의 세대간 전이와 발현 양상을 조사하고자 하였다. 외래 유전자 전이 양상 조사는 제3세대(G2) GFP 형질전환 수탉을 최초 부계로 사용하여 최종적으로 제9세대(G8) 형질전환 닭을 연속적으로 생산하면서 GFP 유전자 전이 양상을 조사하였다. 형질 전환 병아리 유전 분석은 자외선 램프 아래에서 부화한 병아리들의 날개, 부리와 다리에서 녹색형광단백질을 발현하는 병아리들만을 형질전환으로 선발하였다. 형질전환 닭에서 외래 유전자 전이율은 대략 38~58%이었다. 이는 유전자 전이가 멘델의 유전 법칙을 따르고 있음을 보여주고 있다. 연구결과는 GFP 유전자가 유전자 침묵 없이 멘델의 유전 법칙에 따라 다음 세대로 계속 전이와 발현된다는 것을 보여 주고 있다. This study was performed to analyze the generational transmission and the expression of the foreign gene in the GFP transgenic chickens. The transmission rate and the expression of the GFP gene was investigated from the GFP transgenic rooster (G2) as the first founder to the ninth (G8). Analysis of GFP expression in hatched chickens was used the UV lamp. When GFP was expressed in the wings, bill and legs of a chick, the bird only was selected as a transgenic chick. The average transmission rate of the overall transgenic was 38~58%. These results showed that the transmission of the GFP gene in the transgenic chickens in accordance with the laws of Mendel's continues to the next generation without gene silencing.
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코코넛 워터 및 다양한 제조방법을 이용하여 제조한 SrAl2O4:Eu2+의 광학적 특성
박지연,우현주,장기완 한국물리학회 2013 새물리 Vol.63 No.10
Eu2+-doped SrAl2O4 powder phosphors were prepared by using a solid-state reaction, a wet-solid state reaction, and a sol-gel method, all using coconut water. Their structural and morphological features were analyzed by using x-ray diffractometer (XRD) and field emission scanning electron microscopy (FE-SEM). The optical properties of Eu2+-doped SrAl2O4 phosphors were studied by using a photoluminescent spectroscopy (PL) to measure their excitation and emission spectral profiles. The samples prepared by using the solid-state reaction showed 3 8 μm sizes while the samples prepared by usign the wet-solid state reaction and the sol-gel method showed submicron sizes in the range of 0.2 0.5 μm. As the synthesis method and the amount of coconut water added during the preparation of the SrAl2O4:Eu2+ phosphor decide its excitation and emission properties, our samples showed an excitation band with a wide range of 280 460 nm and a broad band emission peak at 520 nm. Eu2가 첨가된 SrAl2O4:Eu2+ 분말형광체를 고상반응법,습식고상반응법 및 졸겔법으로 제조하였다. 제조된 시료의 구조 및형상은 XRD 및 FE-SEM을 이용하여 분석하였으며, 광학적 특성은 여기 및발광스펙트럼 등의 측정을 통하여 분석하였다. 합성방법에 따른결정형태를 분석한 결과 고상반응법으로 제조한 시료는 직경이 3 ~8 μm 의 크기를 가졌으며, 코코넛워터를 첨가한 습식고상반응법 또는졸겔법으로 제조한 시료들의 직경은 약 0.2 ~ 0.5 μm였다. 본연구에서 사용된 시료들의 광학적 특성을 측정한 결과, 모든 시료가 280 ~ 460 nm 의 넓은 여기 파장영역을 나타냈고, 발광스펙트럼은 520nm에서 최대 세기를 나타냈다.
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산화티타늄 나노 입자에 의한 실크 단백질 형광 증폭 연구
Rakesh Kumar Jha,김성환 한국광학회 2024 한국광학회지 Vol.35 No.1
We report a purely protein-based platform for green fluorescence by mixing silk protein with green fluorescence protein (GFP), and also report its enhancement by the incorporation of TiO 2 nanoparticles. The TiO 2 nanoparticles eployed have diameters of 100 and 300 nm, with a significant increase in fluorescence (by a factor of 7.5) observed when introducing 300-nm TiO 2 nanoparticles. Furthermore, an increase in particle distribution density is found to enhance fluorescence amplification. These research findings suggest that protein-based fluorescent films can be enhanced by the characteristics of nanoparticles, opening up new possibilities in the fields of optics and fluorescence applications.
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