들잔디와 금잔디 수집종의 지역별 적응성 및 봉분 실증 평가

장석원,성창현,배은지,구준학,윤정호 한국잔디학회 2019 Weed & Turfgrass Science Vol.8 No.3

Zoysia japonica and Z. matrella are native turfgrass in Korea. Z. japonica is used throughout the country, but Z. matrella is weak in the cold climate and restricted to the southern areas. Z. matrella has a lot of advantages such as fine leaves, high density and excellent texture, so it is important to evaluate the applicability for various purposes. This study was conducted to evaluate the overwintering survival of the Z. japonica 10 and Z. matrella 18 ecotypes collected from across the country in Yangju, Eumseong, and Namhae areas from 2014 to 2015. Each of the two species was also selected and compared with the adaptability as grave covering grass in virtual grave mounds of actual size in Hapcheon, Gyeongnam province from 2017 to 2018. All of Z. japonica ecotypes overwintered in three areas, but 38.9, 83.3, and 88.9% of Z. matrella ecotypes in the Yangju, Eumseong, and Namhae areas survived through winter, respectively. The strip-sodded graves with two species were established in May 2017. In both Z. japonica and Z. matrella graves, ground-coverages were over 90% by Korean thanksgiving day’ mowing (Beolcho), indicating that there was no significant difference between two species in coverage rates. Survival rates of grave-cover plants of Z. japonica and Z. matrella in the following year with over-wintering were 80% and 28%, respectively. Z. japonica suffered damage in the northern slopes of the grave, but Z. matrella plants suffered with great damage in the northern and western slopes. In green-up timing, Z. japonica was faster than Z. matrella, and the slope of the grave proceeded in the order of south>east>west>north. During the winter season, soil temperatures and moisture in the grave remained low in the order of south>east>west>north slopes. 들잔디와 금잔디는 우리나라에서 자생하는 한국잔디이다. 들잔디는 우리나라 전역에서 사용되지만, 내한성이 약한 금잔디는 남부 지방에 제한되어 사용되고 있다. 금잔디는 세엽, 높은 밀도, 우수한 질감 등 많은 장점을 가지고 있어 다양한 용도로서의 가능성을 평가하는 것은 중요하다. 본 연구는 금잔디의 묘지 잔디로서의 활용 가능성을 파악하기 위해 충청도, 경상도, 전라도, 제주도 등지에서 수집한 들잔디 10 계통과 들잔디 18 계통을 3개 지역(경기 양주, 충북 음성, 경남 남해)에서 2014년-2015년 중에 월동 후 생존율과 그린업 시기를 조사하였다. 또한 2017년-2018년에는 금잔디 1계통과 중엽 들잔디 1품종 밀록 을 경남 합천에서 실제 조성한 가상의 봉분에 피복하여 묘지 잔디로서의 적용 가능성을 비교, 평가하였다. 들잔디는 모든 평가 지역에서 월동 후 생존하였으나, 금잔디는 양주, 음성, 남해에서 각각 38.9, 83.3, 88.9%의 개체가 월동하였다. 들잔디와 금잔디 봉분에서는 모두 5월 줄떼 식재 후 9월 추석 전 벌초까지 90% 이상이 피복되어 두 종 사이에는 피복 속도에서 큰 차이를 보이지 않았다. 하지만 들잔디와 금잔디의 월동 후 생존율은 각각 80%와 28%로 큰 차이를 보였다. 들잔디는 봉분의 북쪽 방향에서 동해를 입었으나, 금잔디는 북쪽과 서쪽 방향에서 큰 피해를 입었다. 봉분에서의 그린업은 들잔디가 금잔디보다 빨랐으며, 봉분의 남쪽>동쪽>서쪽>북쪽 방향 순서로 진행되었다. 동계 기간 중 묘지의 토양 온도와 수분은 남쪽>동쪽>서쪽>북쪽 순으로 높게 유지되었다.

한국산 돌나물 수집종의 RAPD에 의한 유연관계 분석

김효진(Hyo-Jin Kim),안정호(Jeong-Ho Ahn),백소현(So-Hyeon Baek),이승엽(Seung-Yeob Lee) 한국원예학회 2008 원예과학기술지 Vol.26 No.1

The genetic relationship among 31 Sedum sarmentosum local strains was analyzed using randomly amplified polymorphic DNA (RAPD) with fifteen 10-mers random primers (Operon Technologies Inc., USA) and twelve 20-mers random primers (SeouLin Bioscience Inc., Korea). Forty-six polymorphic bands (3.3 per primer) and ninety (15.0 per primer) were generated by polymerase chain reaction with selected fourteen 10-mers primers, and six 20-mers primers, respectively. The production of polymorphic bands in 20-mers primers was more efficient than in 10-mers primers. The cluster analysis based on RAPD markers generated by 20-mers primers was matched well with morphological characteristics compared to 10-mers primer. Thirty-one local strains analyzed with UPGMA were clustered into 2 groups. Twenty-seven local strains were clustered into the group Ⅰ, and the geneticrelationship in Group I was very closed except Wanju and Gunsan local strains as no-flowering. Group Ⅱ included Keumsan, Bucheon, Pocheon and Yongin local strains with long hight, slender stem, narrow leaf, and abundant flowering characteristics.

돌나물 수집종간 생육특성, 비타민 C 함량 및 항산화 활성

김효진,이승엽,배종향,정승호 (사) 한국생물환경조절학회 2008 시설원예‧식물공장 Vol.17 No.2

To evaluate growth chracteristics, vitamin C content and antioxidative activity of Sedum sarmentosum, 13 local strains were cultivated in the field. Keumsan, Bucheon, and Pocheon strains had narrow and oblong leaf, long hight, and slender stem compared to the other strains. Wanju and Gunsan strains were not formed flower stalks, and did not flower during the flowering time. Vitamin C contents were ranged from 407.8 to 434.4 mg • kg1, and it was high in Gwangyang, Buan, Wanju, and Gunsan strains. But vitamin C contents didn’t show a significant difference among strains. In antioxidative activity between fresh and freeze dried shoots, electron donating abilities (EDA) were ranged from 45.0 to 66.5% in MeOH extract of fresh shoot, and from 61.3 to 66.5% in MeOH extract of freeze dried shoot. Gwangyang, Suncheon, Wando strains showed high EDA compared to the others. Vitamine C content and antioxidant activity were highest in Gwangyang strain. 돌나물 수집종간 생육특성과 비타민 C 함량 및 항산화활성을 비교하기 위하여, 13 지역종을 포장에 삽목재배하였다. 금산, 부천, 용인, 포천 지역종은 줄기가 가늘고 초장이 길며, 잎은 좁고 긴 타원형으로 다른 지역종들과 형태적 차이를 보였으며, 완주와 군산 지역종은 초장과 절간장이 짧고, 개화기에도 꽃이 피지 않았다. 비타민 C 함량은 407.8 - 434.4mg • kg1의 범위로 광양, 부안, 완주, 군산 지역종에서 높았으나, 수집종간 유의한 차이는 보이지 않았다. 항산화 활성을 나타내는 전자공여능(EDA)은 생체추출물(45.0 ~ 66.5%)에서보다 동결건조 추출물(61.3 ~ 66.5%)에서 높았으며, 광양, 순천, 완도 지역종들이 다른 지역종에 비하여 높은 항산화 활성을 나타내었다. 비타민 C 함량 및 항산화 활성은 광양 지역종에서 가장 높았다.

토경 및 수경재배에서 돌나물의 생육, 비타민 C와 무기성분 함량 변이

이승엽(Seung-Yeob Lee),김효진(Hyo-Jin Kim),배종향(Jong-Hyang Bae) 한국원예학회 2011 원예과학기술지 Vol.29 No.3

The growth, yield and quality of Sedum sarmentosum between soil and hydroponic cultivation were compared to produce high-quality shoots in summer season. The plants were collected from four places in Korea and cultivated in a plastic film house with 50% shading. A 1:1:1 mixtures of sand, upland soil and substrate (N-P-K: 330-220-400 ㎎ㆍℓ?¹) was used for soil cultivation, and a nutrient film technique (NFT) with Yamazaki-lettuce nutrient solution used for hydroponics. After 30 days of cultivation, plant height ranged from 18.4 to 21.8 ㎝ and showed no significant difference between soil and hydroponic cultivation. Number of shoot, leaf length, leaf width, number of leaf, fresh weight, and dry weight significantly increased in hydroponics compared to those in soil cultivation. Fresh and dry weights in hydroponics were greater in ‘Gunsan’ and ‘Wando’ collections than those in other collections. Stem diameter and compression-force significantly increased in soil cultivation rather than hydroponics with significant differences among collections. However there were no significant differences in shoot bitterness and vitamin C content between cultivation methods or among the collections. Most mineral contents except Fe in hydroponics were higher than those in soil cultivation. K, Na, Zn contents in hydroponics were over 2 times higher than those in soil cultivation.

돌나물 수경재배에서 재식밀도에 따른 생육 및 품질 특성

이승엽(Seung-Yeob Lee),김효진(Hyo-Jin Kim),배종향(Jong Hyang Bae) 한국원예학회 2010 원예과학기술지 Vol.28 No.4

The effect of planting densities on the growth of Sedum samentosum (4 collections) was investigated using nutrient film technique (NFT) with Yamazaki' lettuce nutrient solution (1982) from September 5th to October 24th in non-heating plastic film house. At 40 days, the plant height of 4 collections showed the range of 15.0-18.9 ㎝, and mean plant height was high the order of 10×10, 2.5×2.5, and 5×5 ㎝. The growth of ‘Pohang’ and ‘Wando’ collections was better compared to that of ‘Gunsan’ and ‘Wanju’ collections. Number of node, stem diameter, and fresh and dry weight per plant were decreased in higher planting density. Despite the lower fresh weight per plant obtained, the fresh yield per ㎡ was significantly increased in higher planting density. The mean fresh yield was 14.9 ㎏ㆍm?² in 2.5×2.5 ㎝, and ‘Pohang’ collection showed the highest fresh yield (17.6 ㎏ㆍm?²). The first optimized harvesting time base on plant height was 30-40 days after NFT culture during autumn season. In eating quality, compression force of stem and bitterness of shoot were decreased in higher planting density. However, the heavy labor demanding high density cutting needs to be improved for hydroponic culture of S. sarmentosum.

RAPD를 이용한 대파의 품종분류

우서(Seo Woo),노일섭(Ill-Sup Nou),양승렬(Seung-Yul Yang),은무영(Moo-Young Eun),차영순(Young-Soon Cha),윤도원(Doh-Won Yun) 한국원예학회 2008 원예과학기술지 Vol.26 No.4

RAPD analysis was used to determine the genetic relationship of 24 welsh onion (Allium fistulosum L.) accessions. Fourteen random primers were tested and two primers which showed polymorphism were selected. The amplified fragments ranged from 0.10 to 2.00 kb in size. The total number of polymorphic bands scored was 54 (19%). From cluster analysis, 24 accessions were separated into three major groups: Nineteen out of 24 accessions that belonged to ‘Seol Poong’ groups were divided into four subgroups. Also three out of 24 accessions that belonged to ‘Kincho’ groups were divided into two subgroups. And two out of 24 accessions that belonged to ‘Kincho 3go’ groups were divided into two cultivars.

국내 자생 돌나물의 형태적 특성에 의한 유연관계 분석

김효진(Hyo Jin Kim),이승엽(Seung Yeob Lee) 한국원예학회 2007 원예과학기술지 Vol.25 No.2

For the analysis of genetic relationship among genetic resources of Sedum sarmentosum, the morphological characteristics were investigated in 31 Korean local strains, and cluster analysis using SAS program (version 8.2) was conducted based on the morphological data. Thirty-one local strains were classified into two groups by the cluster analysis based on growth and flowering characteristics. Group Ⅰ included most of local strains with flowering characteristic, and Group Ⅱ included non-flowering, ‘Wanju’ and ‘Gunsan’ local strains. Group Ⅰ was divided into two subgroups. Four local strains with flourishing growth and flowering characteristics, ‘Keumsan’, ‘Bucheon’, ‘Pocheon’, and ‘Yongin’ were contained into the subgroup Ⅰ-1, and others were belonged into the subgroup Ⅰ-2. Among 31 local strains, ‘Wanju’ and ‘Gunsan’ local strains with good growth and non-flowering characteristics were favorable as edible resources. It was desirable as the resources of garden plant and ground cover plant that ‘Keumsan’, ‘Bucheon’, ‘Pocheon’, and ‘Yongin’ local strains showed the characteristics of slender stem and leaf, long flower stock, and flourishing flowering.