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Sung Won Kim,Jung Sook Lee,Seung Gyu Park,Han Ju Kang,Yong Soo Kim,Young Dae Yoon,Hoe Song Yang,Han Gi Lee,Sang Soo Kim 국제물리치료학회 2010 Journal of International Academy of Physical Ther Vol.1 No.1
This study was performed to investigate the effects of Needle Electrode Electrical Stimulation(NEES) on ischemia-induced cere˗ brovascular accidents. After obstruction and reperfusion of arteries in white mice, the amounts of necrosis and inflammation related sub˗ stances Bax, IL-6, Caspase-3, and COX-2 were measured in neurons of the fore-brain. The following results were obtained. This study used 21 male specific pathogen free(SPF) SD rats, 8 weeks of age and approximately 300g in weight. Each exposed artery was completely occluded with non-absorbent suture thread and kept in that state for 5 minutes. The sutures were then removed to allow reperfusion of blood. Test group is control group(common carotid artery occlusion models), a GI(underwent common carotid artery occlusion), and NEES(underwent NEES after artery occlusion). The GI and NEES groups were given 12, 24, or 48 hours of reperfusion before NEES. NEES device(PG6, ITO, Japan, 9V, current, 2Hz) was used to stimulate the bilateral acupoint ST36 of the SD rats for 30 minutes while they were sedated with 3% isoflurane. An immuno-his˗ tochemistry test was done on the forebrains of the GI induced rats. Both Bax and Caspase-3 immuno-reactive cells, related to apoptosis, were greater in the GI than the NEES group. Cox-2 and IL-6 immuno-reactive cells, related to inflammation, were greater in the GI and NEES groups than the control group. We can expect that applying NEES after ischemic CVA is effective for preventing brain cells from being destroyed. And we can conclude NEES should be applyed on early stage of ischemic CVA.
Kim, Sung-Won,Lee, Jung-Sook,Park, Seung-Gyu,Kang, Han-Ju,Kim, Yong-Soo,Yoon, Young-Dae,Yang, Hoe-Song,Lee, Han-Gi,Kim, Sang-Soo International Academy of Physical Therapy Research 2010 Journal of International Academy of Physical Ther Vol.1 No.1
This study was performed to investigate the effects of Needle Electrode Electrical Stimulation(NEES) on ischemia-induced cerebrovascular accidents. After obstruction and reperfusion of arteries in white mice, the amounts of necrosis and inflammation related substances Bax, IL-6, Caspase-3, and COX-2 were measured in neurons of the fore-brain. The following results were obtained. This study used 21 male specific pathogen free(SPF) SD rats, 8 weeks of age and approximately 300g in weight. Each exposed artery was completely occluded with non-absorbent suture thread and kept in that state for 5 minutes. The sutures were then removed to allow reperfusion of blood. Test group is control group(common carotid artery occlusion models), a GI(underwent common carotid artery occlusion), and NEES(underwent NEES after artery occlusion). The GI and NEES groups were given 12, 24, or 48 hours of reperfusion before NEES. NEES device(PG6, ITO, Japan, 9V, current, 2Hz) was used to stimulate the bilateral acupoint ST36 of the SD rats for 30 minutes while they were sedated with 3% isoflurane. An immuno-histochemistry test was done on the forebrains of the GI induced rats. Both Bax and Caspase-3 immuno-reactive cells, related to apoptosis, were greater in the GI than the NEES group. Cox-2 and IL-6 immuno-reactive cells, related to inflammation, were greater in the GI and NEES groups than the control group. We can expect that applying NEES after ischemic CVA is effective for preventing brain cells from being destroyed. And we can conclude NEES should be applyed on early stage of ischemic CVA.
이정숙 ( Jung Sook Lee ),김병기 ( Byung Ki Kim ),김성원 ( Sung Won Kim ),김지성 ( Ji Sung Kim ),김동일 ( Dong Il Kim ),송치원 ( Chi Won Song ) 대한한방신경정신과학회 2009 동의신경정신과학회지 Vol.20 No.2
Objectives: This study was performed to investigate the effects of Needle Electrode Electrical Stimulation (NEES) on ischemia-induced cerebrovascular accidents. After obstruction and reperfusion of ** arteries in white mice, the amounts of necrosis and inflammation related substances IL-6, Caspase-3, and PARP, C-fos were measured in neurons of the hippocampus. The following results were obtained. Methods: This study used 21 male specific pathogen free (SPF) SD (Sprague Dawley) rats, 8 weeks of age and approximately 300 g in weight, that were given at least 1 week to adapt to the lab environment Each exposed artery was completely occluded with non-absorbent suture thread and kept in that state for 5 minutes. The sutures were then removed to allow reperfusion of blood. Test group is control group for comparison with the common carotid artery occlusion models, a GI group that underwent common carotid artery occlusion, and a needle electrode electrical stimulation (NEES) group that underwent NEES after artery occlusion. The GI and NEES groups were given 12, 24, or 48 hours of reperfusion before NEES. NEES device (PG6, ITO, Japan, 9V, current, 2Hz) was used to stimulate the right and left acupoint ST36 of the SD rats for 30 minutes while they were sedated with 3% isoflurane. An immunohistochemistry test was done on the forebrains of the GI induced rats. All the data collected from this study was symbolized and analyzed using a statistics processing program (SPSS 12.0K/PC). The level of significance was set at α=0.05 and a T-TEST analysis was used to find out the effects of treatment on each of the groups: the normal group, the CVA induced group, and the treatment after CVA induction group. Results: Both PARP and C-fos immuno-reactive cells, related to apoptosis, were greater in the GI groups than the NEES group. Caspase and IL-6 immuno-reactive cells, related to inflammation, were greater in the GI and NEES groups than the control group. Conclusions: This research was conducted to study the effects of NEES on CVA due to ischemia. Occlusion and reperfusion was performed on the common carotid arteries of white rats, after which amounts of substances related to neuron necrosis and inflammation-PARP, IL-6, Caspase-3, and C-fos - were measured in the Hippocampus.
Jung Hyun Choi 국제물리치료연구학회 2011 Journal of International Academy of Physical Ther Vol.2 No.2
This research was attempted to seek for a positive approach within the framework of physical therapy instead of the drug treatment in the past, with regard to the ischemic brain injury in the early stage. Accordingly, the aim of this research is to observe the change of HSP27 and HSP70, the genes that are expressed in the early stage of brain injury and to investigate the effects of needle electrode electrical stimulation(NEES), upon applying NEES after ischemia. The experimental method is to give rise to global ischemia and apply NEES to 27 SD-Pat rats with the particulars of being eight-week-old, male, around 300g, and adapted to laboratory environment for more than a week, and divide them into three groups, that is, GV20 NEES group(n=9), L14 NEES group(n=9), no applied NEES global ischemia(GI) group(n=9), and then observe their changes of HSP27 and HSP70 at the time lapse of 6, 9 hr and 12 hr after ischemia, using immunohistochemistry methods. Upon observing through the immunohistochemistry method, it was noticed that there was a significant difference between the GV20 NEES group and the L14 NEES group as for HSP27 and there were significant differences among all groups as for HSP70(p<.05). Accordingly, it is supposed that the application of NEES after the outbreak of cerebral ischemia delay the apoptosis in the early ischemic part of forebrain or protect neurons against apoptosis.
Choi, Jung-Hyun International Academy of Physical Therapy Research 2011 Journal of International Academy of Physical Ther Vol.2 No.2
This research was attempted to seek for a positive approach within the framework of physical therapy instead of the drug treatment in the past, with regard to the ischemic brain injury in the early stage. Accordingly, the aim of this research is to observe the change of HSP27 and HSP70, the genes that are expressed in the early stage of brain injury and to investigate the effects of needle electrode electrical stimulation(NEES), upon applying NEES after ischemia. The experimental method is to give rise to global ischemia and apply NEES to 27 SD-Pat rats with the particulars of being eight-week-old, male, around 300g, and adapted to laboratory environment for more than a week, and divide them into three groups, that is, GV20 NEES group(n=9), L14 NEES group(n=9), no applied NEES global ischemia(GI) group(n=9), and then observe their changes of HSP27 and HSP70 at the time lapse of 6, 9 hr and 12 hr after ischemia, using immunohistochemistry methods. Upon observing through the immunohistochemistry method, it was noticed that there was a significant difference between the GV20 NEES group and the L14 NEES group as for HSP27 and there were significant differences among all groups as for HSP70(p<.05). Accordingly, it is supposed that the application of NEES after the outbreak of cerebral ischemia delay the apoptosis in the early ischemic part of forebrain or protect neurons against apoptosis.
Sung-Won Kim,Byung-Ki Kim,Jung-Sook Lee,Ji-Sung Kim,Chi-Won Song 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.4
The aim of this study to investigate the effects of needle electrode electrical stimulation (NEES) on ischemia-induced Common Carotid Artery Occlusion. This Ischemia model was used rat brain that completely occluded with non-absorbent suture thread and kept for 5 minutes. Then suture were removed to allow the reperfusion of blood. The test groups were divided into three; the control group without common carotid artery ligation, the GI group in which global ischemia was induced through occlusion of common carotid artery and the NEES group in which NEES used after the global ischemia. NEES was conducted at the meridian points called Choksamni (ST 36) at 12, 24, 48 hours after blood reperfusion. The immune-reactive cells both Bax and caspase-3 indicates the apoptosis were high in the group GI comparing to NEES group. Cox-2 and IL-6 indicates the inflammation were high in the GI comparing to NEES groups. These results suggest that the NEES treatment has the beneficiary effects on ischemia.
Kuppusamy CHENNIAPPAN,Murugan KADARKARA 한국곤충학회 2008 Entomological Research Vol.38 No.2
Oviposition is an important phenomenon of mosquitoes and has recently become the focus in the concept of integrated vector control management. In the present study, we evaluated oviposition deterrent, ovicidal and mortality effects of ethanolic extract of Andrographis paniculata Nees against gravid and oviposited females of Anopheles stephensi Liston. Water treated with the ethanolic extract had a high deterrent activity in ovipositing females: oviposition activity index values for the test species were -0.28, -0.45, -0.49 and -0.59 for extract concentrations of 29, 35, 41 and 46 p.p.m., respectively. High degrees of mortality were observed with various concentrations of extract: 1.12 (control) to 11.70 for gravid females, and 0.65 (control) to 10.25 for oviposited females. The highest mortality in both gravid and oviposited females was observed soon after they came in contact with oviposition medium treated with the extract, and this was found to be significant at doses higher than 35 p.p.m., suggesting possible contact toxicity of the extract. The extract caused moderate ovicidal activity against various age groups of A. stephensi, but it inflicted delayed effects such as high larval, pupal and adult mortality. The age of the eggs and the duration of the extract treatment influenced the ovicidal activity observed. It is clear that ethanolic extract of A. paniculataNees can affect the oviposition cycle of A. stephensi Liston, thereby suppressing the vector populationand adversely influencing transmission of the disease pathogen.
구조실험정보를 위한 데이터 모델의 개발 및 구현 과정에 대한 분석
이창호(Lee Chang-Ho) 대한건축학회 2010 大韓建築學會論文集 : 構造系 Vol.26 No.7
The data models for the structural experiments organize formally the information involved in the structural experiments, and the formally organized information is used for implementing the computer systems. Previous research on the data models has provided various data models for their own application fields of the structural experiments, but their development methods has not been carefully reviewed and discussed. This paper analyzes the development and implementation procedure of the data models for structural experiments including the Lehigh Model for large-scale and hybrid structural experiments, the NEES data model for general categories of experiments, and the KOCED data model for distributed and shared experiments. Based on the analysis, some noticeable suggestions are given to be considered for developing the comprehensive data models for the structural experiments. The domain of the data models should be clearly defined at the initial stage of the development procedure. The categories and the levels of the information need to be decided considering the characteristics of the structural experiments. The most significant part of a data model is to be formulated first because the initial formulation affects the rest of the development procedure and the data model evolves during the development procedure.
Resistance mechanism of Leptochloa chinensis Nees to propanil
Sahid, Ismail B.,Karso, Juliana,Chuah, Tse-Seng The Korean Society of Weed Science and The Turfgra 2011 Weed Biology and Management Vol.11 No.2
The resistance mechanism of Leptochloa chinensis Nees to propanil was investigated, based on propanil metabolism, aryl acylamidase activity, and chlorophyll fluorescence at the 8 week growth stage of L. chinensis. The concentration of propanil in the leaf and culm extracts of the resistant (R) and susceptible (S) biotypes, as measured by gas chromatography (GC), was found to increase after propanil treatment.The concentration of propanil in the leaf and culm extracts of the S biotype at 72 h was 1.55 and 0.49 ${\mu}g\;mL^{-1}$, respectively. However, a lower concentration of propanil was observed in the R biotype, as compared to that in the S biotype. The residue of 3,4-dichloroaniline, as measured by GC, was detected only in the leaf extracts of the R biotype. In contrast, no residue of 3,4-dichloroaniline was observed in the S biotype. The level of aryl acylamidase in the leaf tissue extracts of the R biotype was ~140% higher than that in the S biotype. The fluorescence studies showed that propanil inhibited the quantum efficiency of the photosystem II in both the R and S biotypes after 2 h of incubation time. However, when the leaf disks were transferred and incubated in deionized water for 48 h, the quantum efficiency increased in the R biotype but decreased in the S biotype. These results suggest that propanil metabolism, enhanced by aryl acylamidase activity, is the most likely factor contributing towards the mechanism of propanil resistance in L. chinensis plants at the 8 week growth stage.
데이터 모델을 이용한 실험정보 저장소의 실험정보 구성 평가 및 개선
이창호(Lee, Chang-Ho) 대한건축학회 2017 大韓建築學會論文集 : 構造系 Vol.33 No.6
The experiment information repository includes the information related with experiment projects. After the experiment information repository is implemented into a computer system and made to be accessible through the internet, the experiment information in the repository can be more easily used by engineers, compared with technical papers or experiment reports. This paper uses the classes and attributes of the data model to represent the organization of the experiment information of the experiment information repository, and presents some criteria to evaluate the usage of the classes and attributes for the experiment projects. The class usage ratio and the attribute usage ratio are useful to identify which part of the experiment information for each project is missing. The class family usage ratio helps to understand the information stored in the class including lower level classes. These evaluation criteria are applied into the NEES and KOCED repositories, and then some rules are proposed to improve the organization of the experiment information. An example of the rules is that the attribute of a class whose attribute usage ratio is low should be removed, or needs to be reorganized if the attribute includes at its lower level the class whose usage ratio is high. The usage evaluation criteria and the rules can be applied to develop other types of information repositories.