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Ghimire, B.K.,Seong, E.S.,Yu, C.Y.,Kim, S.H.,Chung, I.M. Bureau for Scientific Publications, Foundation for 2017 South African journal of botany : official journal Vol.109 No.-
<P>This study was carried out to investigate antimicrobial activities of leaf and root extracts of transgenic Codonopsis lanceolata with over-expressing gamma-tocopherol methyl transferase (gamma-tmt) gene. The antimicrobial activity was assessed using the agar disc diffusion method and minimum inhibitory concentration (MIC) method, and the extracts from the transgenic C. lanceolata were compared with those of the wild plants by using the in-vitro antimicrobial assays with five pathogenic bacteria and two yeast species. The results indicated that transgenic C. lanceolata overexpressing gamma-tmt possesses antimicrobial activity against both gram-positive and gram-negative bacteria. Transgenic lines showed increased antimicrobial activity, ranging from 1.90 +/- 0.50 to 5.50 +/- 0.77 mm in the leaf extract of transgenic C. lanceolata, compared to the extract from the control plants, ranging from 1.27 +/- 0.50 to 4.73 +/- 0.40 mm. Staphylococcus aureus was the most susceptible microorganism to transgenic leaf extracts with lowerMICs (500 mu g/mL) and higher growth inhibition zones (5.33mm). Leaf extract of transgenic plants showed antimicrobial activity against Salmonella typhimurium, Klebsiella pneumoniae, and Escherichia coli. However, transgenic root extract showed lower antimicrobial activity against all tested microorganisms, with MIC > 1000 mu g/mL. The a-tocopherol content of the plants was enhanced by the overexpression of (gamma-tmt) gene compared to control plant. We observed an increase in the phenolic compound concentration in transgenic leaves. The predominant phenolic compounds that increased in the transgenic leaf were chlorogenic acid, luteolin, benzoic acid, and apigenin. The antimicrobial activity of transgenic C. lanceolata was strongly correlated to the concentration of phytochemical compounds. These results may help to identify novel natural antimicrobial agents that could be used against infectious diseases. (C) 2016 SAAB. Published by Elsevier B.V. All rights reserved.</P>
Shin, S.Y.,Kim, I.S.,Kim, Y.S.,Lee, H.,Yoon, H.S. Bureau for Scientific Publications, Foundation for 2013 South African journal of botany : official journal Vol.88 No.-
Ascorbate (vitamin C) plays an important role in detoxification of reactive oxygen species (ROS) in most living organisms. Monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) is crucial to regeneration of the oxidized form of ascorbate (monodehydroascorbate) so that it can be recycled to maintain ROS scavenging ability. The MDHAR gene from Brassica rapa L. was cloned and introduced into Arabidopsis thaliana (L.) Heynh. to test the hypothesis that enhanced ROS scavenging activity of BrMDHAR alleviates freezing stress. BrMDHAR was expressed under the control of either the CaMV 35S promoter or stress inducible SWPA2 promoter. Ectopic expression of BrMDHAR led to the up-regulation of many antioxidant genes, including APX, DHAR, GR, SOD, GPX, and PRX Q, which are involved in ascorbate-glutathione cycle. And, transgenic plants showed improved stress tolerance against freezing with exhibiting higher levels of chlorophyll content and antioxidant molecules such as ascorbate and glutathione as well as alleviated redox status and malondialdehyde contents. These results suggested that ectopic expression of BrMDHAR conferred improved tolerance to freezing stress not only by simply recycling ascorbate, but also by inducing co-regulation of the ascorbate-glutathione cycle, which in turn enhances the antioxidant capacity of the host plants.