http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
KIM, SANG-HUN,KIM, KWANG-YOUN,YU, SUN-NYOUNG,PARK, SUL-GI,YU, HAK-SUN,SEO, YOUNG-KYO,AHN, SOON-CHEOL Anticancer Research USA Inc. 2016 Anticancer research Vol.36 No.11
<P>Background: Monensin is a carboxyl polyether ionophore that potently inhibits the growth of various cancer cells. Recently, the anticancer effects of monensin have been recognized based on its ability to induce apoptosis in cancer cells. However, anticancer effect of monensin and its mechanism of action have yet to be investigated, especially against human prostate cancer cells. Materials and Methods: Cell viability assay, western blot, cell-cycle arrest, annexin V/propidium iodide assay, reactive oxygen species (ROS) production and intracellular Ca2+ flux were assayed. Results: In this study, monensin significantly inhibited cell viability in a dose-dependent manner in prostate cell lines. Moreover, cell growth inhibition by monensin induced G(1) phase cell-cycle arrest and apoptosis via regulation of cell cycle-and apoptosis-related proteins in PC-3 cells. In addition, monensin induced the production of ROS and the disruption of Ca2+ homeostasis, that was restored by diphenyleneiodonium, a mitochondrial ROS inhibitor and verapamil, a Ca2+ channel blocker, respectively, as confirmed by pro-caspase-3 activation and poly ADP ribose polymerase cleavage. Conclusion: Monensin induces cell-cycle arrest and apoptosis through regulation of cell cycle-and apoptosis-related proteins, resulting in induction of mitochondrial ROS-and Ca2+-dependent apoptosis, respectively.</P>
YUN, CHUL WON,YUN, SEUNGPIL,LEE, JUN HEE,HAN, YONG-SEOK,YOON, YEO MIN,AN, DANIEL,LEE, SANG HUN Anticancer Research USA Inc. 2016 Anticancer research Vol.36 No.9
<P>Background: The putative functions of the cellular prion protein (PrPc) are believed to be associated with cell signaling, differentiation, survival, and cancer progression. With respect to cancer development and progression, elevations and mutations of PrPc expression have been shown to increase the risk for malignancy and metastasis in breast and colorectal cancer. Since both natural supplements and direct regulation of PrPc expression contribute to inhibition of cancer progression and growth, we hypothesized that knockdown of PrPc could lead to an enhanced synergic effect on the inhibition of cancer growth by fucoidan. Materials and Methods: PrPc expression was suppressed in HT29 human colon cancer cells by utilizing small-interfering RNA (si-PRNP), and cells were subsequently used to study the antiproliferative and anticancer effects of fucoidan treatment of HT29 human colon cancer cells. Results: Fucoidan treatment significantly inhibited growth and reduced cyclin and cyclin-dependent kinase (CDK) expression in HT29 colon cancer cells. Furthermore, silencing PrPc expression with si-PRNP amplified the fucoidan-induced changes in cell proliferation, apoptosis, and migration. Intraperitoneal injection of si-PRNP with fucoidan reduced proliferation and tumor volume in Balb/c nude mice. This enhanced antitumor efficacy was associated with decreased angiogenesis. Conclusion: Combination of fucoidan with silencing of PrPc has a synergic effect on the inhibition of HT29 colon cancer cell growth. Furthermore, we provide evidence for the therapeutic application of PrPc silencing with other anticancer drugs for cancer.</P>
KWON, OH KWANG,JEON, JU MI,SUNG, EUNJI,NA, ANN-YEA,KIM, SUN JOO,LEE, SANGKYU Anticancer Research USA Inc. 2018 Cancer genomics & proteomics Vol.15 No.4
<P>Background: Secreted proteins play an important role in promoting cancer (PCa) cell migration and invasion. Proteogenomics helps elucidate the mechanism of diseases, discover therapeutic targets, and generate biomarkers for diagnosis through protein variations. Materials and Methods: We carried out mass a spectrometry-based proteomic analysis of the conditioned media (CM) from two human prostate cancer cell lines, belonging to different metastatic sites, to identify potential metastatic and/or aggressive factors. Results: We identified a total of 598 proteins, among which 561 were quantified based on proteomic analysis. Among the quantified proteins, 128 were up-regulated and 83 were down-regulated in DU145/PC3 cells. Six mutant peptides were identified in the CM of prostate cancer cell lines using proteogenomics approach. Conclusion: This is the first proteogenomics study in PCa aiming at exploring a new type of metastatic factor, which are mutant peptides, predicting a novel biomarker of metastatic PCa for diagnosis, prognosis and drug targeting.</P>
Casein Kinase 2 Inhibitor, CX-4945, as a Potential Targeted Anticancer Agent in Gastric Cancer
KIM, HYUN MYONG,JEONG, INHYE,KIM, HYUN JEONG,KANG, SUN KYOUNG,KWON, WOO SUN,KIM, TAE SOO,PARK, KYU HYUN,JUNG, MINKYU,SOONG, JOHN,LIN, SHU-CHUAN,CHUNG, HYUN CHEOL,RHA, SUN YOUNG Anticancer Research USA Inc. 2018 Anticancer research Vol.38 No.11
LEE, YOON HEE,KIM, MIN KYUNG,MOON, HEE YOUNG,CHONG, GUN OH,LEE, HYUN JUNG,LEE, YOON SOON,PARK, JI YOUNG,LEE, CHAN HYEONG,BAEK, MOON CHANG,HONG, DAE GY Anticancer Research USA Inc. 2018 Cancer genomics & proteomics Vol.15 No.4
<P>Background/Aim: This study was designed to identify candidate proteins which can be used for visualization of pelvic autonomic nerve during nerve-sparing surgery. Materials and Methods: Both soft tissue and vesical branch of the inferior hypogastric nerve from five women were collected during surgery. These 10 tissue specimens were analysed using liquid chromatography-mass spectrometry (LC-MS) and Human Protein Atlas (HPA) for protein expression. The existence of nerve fibres was confirmed using haematoxylin and eosin (H&E) and anti-S-100 staining. Results: A total of 413 proteins were detected. There were three proteins (isoform 1 of fibronectin, protein S100-A8 and A9) which implied a relation with pelvic autonomic nerve. In nerve tissue from one case, the existence of nerve fibre was not confirmed. Conclusion: Further large studies are expected to present more nerve-specific candidate proteins which can be used for the easy and safe identification of autonomic nerves.</P>