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인간배아 줄기세포로부터 분화된 혈관내피세포의 분리 및 in vivo 실험을 통한 조직재생 가능성 평가
김주미 ( Ju Mi Kim ),문성환 ( Sung Hwan Moon ),이수홍 ( Soo Hong Lee ),이동률 ( Dong Ryul Lee ),정형민 ( Hyung Min Chung ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.4
Endothelial cells differentiated from human embryonic stem cells(hESCs) were efficiently obtained and applied to in vivo cell study. As hESCs differentiated via embryoid bodies(EBs) spontaneously, endothelial cells, PECAM positive cells were expressed in the middle part of EBs. Thus by attaching EBs on the dish, the middle part of EBs was able to be isolated. Isolated cells were characterized as endothelial cell by using immunostaining and FACS analysis. Using immunostaining and FACS analysis, we have shown that expression of various endothelial cell markers, including PECAM, vWF and growth factor receptors Tie-2. For in vitro functional test of differentiated endothelial cells, we performed matrigel assay and uptake of ac-LDL. For in vivo study, endothelial cells sorted by FACS were encapsulated by thermo-sensitive PNiPAAm solution and then injected into subcutaneous in nude mice. After 4 weeks of cell injection, hydrogel were analyzed by immunostaining such as endothelial cell specific maker(Tie-2) and human specific nuclei. The endothelial cells were investigated in hydrogels suggesting the cells in hydrogel are possibly able to survive, proliferate and furthermore form vessel like tissue.
안면부의 조직공학적 재생을 위한 발생영역 수복에 대한 고찰
박정민 ( Jung Min Park ),서미현 ( Mi Hyun Seo ),김성민 ( Soung Min Kim ),김선아 ( Seon Ah Kim ),명훈 ( Hoon Myoung ),이종호 ( Jong Ho Lee ) 한국조직공학과 재생의학회 2013 조직공학과 재생의학 Vol.10 No.1s
In growing child, corrective surgery for congenital malformation can cause complication and problems, such as repetitive relapse and growth disturbance over time. These are because the biologic rationale of the procedure is incorrect, and the anatomic pattern of relapse points an accusatory finger at the pathology. Recent updates in surgical approach for the clefting are focused on facial development and origin of clefting. Successful treatment of congenital craniofacial defect can be acquired by understanding of developmental processes. The developmental field repair (DFR) is based on the neuromeric model, which propose facial tissues are originated from embryonic nervous system. This concept consider the identification and reassignment specific developemental field. Goals of DFR surgery are 1) resolution of all pathologic processes of clefting, 2) dissection following the embryonic separation plane, 3) preservation of vascular supply for alveolar mucoperiosteum, 4) primary unification of alveolus, 5) reassignment of all developmental field to correct relation. Therefore, understanding of developmental field theory must be proceeded surgical approach. This concepts are based on reconstruction of deformed tissues following embryonic origin, which are able to be functional and anatomical repair. Therefore, in this article the author reviewed the developmental neuromeric theory, and the developmental field repair concept based on this neuromeric model.
저출력 레이저의 생자극효과를 이용한 조직재생에 관한 최신연구동향
강경리 ( Kyung Lhi Kang ),박준봉 ( Joon Bong Park ),지유진 ( Yu Jin Jee ),류동목 ( Donk Mok Ryu ),이덕원 ( Deok Won Lee ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Nowadays laser gets attention again as a novel method in various treatment fields, such as oral surgery, endodontic and periodontal treatment, etc. Also biostimulation is an special area that we can`t pass over. Biostimulation means that low-level laser irradiation promotes the regeneration process in bone, soft tissue, and peri-implant tissue. Although the mechanism has not been elucidated, many reports have supported the biostimulation effect and it is generally accepted. In this feature article, researches up to now were reviewed to know the trends and future research focus.
자가혈액에서 농축한 골유도성장인자의 임상응용과 개발의 최근동향
이덕원 ( Deok Won Lee ),지유진 ( Yu Jin Jee ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
골 형성을촉진하는 물질로는 골유도단백(BMP), 혈소판 유래 성장인자(PDGF), 형질 전환 성장인자(TGF-β) 등의 다양한 골 형성 기질 등이 있다. 이러한 성장인자는 치유 과정시 조직의 재생에 필수적인 신생혈관의 형성, 세포의 화학주성 및 분열촉진, 간세포 증식, 기질합성 등을 조절하는 인자로 조직재생에 기여한다. 최근 조직공학 연구가 발전하면서 이러한 국소적 역할을 하는 성장요소들에 대하여 활발히 연구되고 있다. 이러한노력 중 하나가 혈액내 혈소판에 존재하는 여러 성장인자를 임상적으로 이용하고자 혈소판을 농축하여 적용하는 혈소판풍부혈장(PRP)과 혈소판풍부 피브린(PRF) 이다 . PRP와 PRF는 방법의 용이성과 효능면에서 많은 장점을 가지고 있기에 이에 대한 관심과 연구가 지속되고 있다. 하지만 아직 임상적 적용에 대하여 학문적 고찰과 개선이 필요하므로 이에 대한 문헌고찰과 최근의 연구를 살펴보고자 한다.
발생과정에서 연골형성의 조절과 이의 연골조직공학에의 응용
김소영 ( So Young Kim ),임군일 ( Gun Il Im ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
연골은 중배엽성 기원의 조직으로서 성체에서 관절의 윤활 및 충격흡수기능을 담당한다. 관절연골은 손상 후 잘 재생이 되지 않으므로 조직공학적인 방법을 통하여 연골의 재생을 시도하려는 연구가 많이 진행되고 있다. 발생과정에서 연골이 생성되는 과정을 잘 이해한다면, 즉 연골분화과정을 조절하는 외인성 요인(환경적)및 내재성 요인(유전적 조절)에 관한 정확한 기전을 안다면 이를 연골조직공학에 적용하여 보다 성공적인 인공연골을 개발할 수 있을 것이다. 본고에서는 발생과정중의 연골분화과정 중에 발생하는 형태유전적인 변화 및 조절기작과 연골의 전사조절인자 및 연관된 유전자의 역할, 간엽 응축 및 연골의 성숙에 있어 세포외기질의 역할을 정리하고 발생과정의 연골분화를 모방할 것으로 추정되는 성체연골모전구세포에 대한 최신 지견을 요약하며 또한 연골재생에 최근에 적용되고 있는 새로운 생체재료들과 그 적용에 대해서 논의하고자 한다.
전소영 ( So Young Chun ),이효정 ( Hyo Jung Lee ),최영애 ( Young Ae Choi ),김재영 ( Jae Young Kim ),조제열 ( Je Yeol Cho ),신홍인 ( Hong In Shin ),박의균 ( Eui Kyun Park ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
This review focuses on the current understanding of tooth regeneration using embryonic tooth budderived tissues/cells and adult stem cells, and tissue engineering technologies combined with biomaterials. Tooth development is achieved by sequential and reciprocal interactions between the oral epithelium and the mesenchyme. Especially growth factors, transcription factors and extracellular matrix proteins play key roles during tooth development process. To achieve successful bio-engineered tooth, several different sources for epithelium and mesenchymal stem cells are needed to be assessed their potential efficacy. Next, discussion focuses on cell culture and implantation methods, particularly, medium supplements, gene transfection, chimeric tooth bud and optimized implant regions. At the end, we introduce the various biomaterials designed via biomimetic strategies for tooth regeneration applications. Although currently these approaches have several limitations including abnormal morphology of bio-engineered tooth, the tissue engineering approaches for dental regeneration using stem cells is the significant challenge in dental field. Therefore, the knowledge of the stem cells as well as the biomaterials will help our understanding for the tooth regeneration.
골수간엽줄기세포와 폴리글리콜라이드 부직포지지체를 이용한 조직공학적 척추신경재생
김선경 ( Sun Kyung Kim ),홍금덕 ( Keum Deok Hong ),장지욱 ( Ji Wook Jang ),이상진 ( Sang Jin Lee ),김문석 ( Moon Suk Kim ),강길선 ( Gil Son Khang ),이일우 ( Il Woo Lee ),이해방 ( Hai Bang Lee ) 한국조직공학과 재생의학회 2004 조직공학과 재생의학 Vol.1 No.2
Bone marrow stromal stem cells (BMSCs) were harvested from the femurs and tibias of adult female Fischer rat. BMSCs were suspended at 2 × 107 cell/mL in a 25% Pluronic F127 (PF127) solution and then polyglycolide (PGA) nonwoven was saturated with 50 μl PF127 solution. The ability of the PGA/PF127 with BMSCs to be integrated and to promote nerve regeneration in the transected rat spinal cord was investigated. Fischer rat received an implant consisting of the BMSCs suspended in the PGA/PF127 in the gap (T8~T9;~5 mm) created by the spinal cord resection. For histological evaluation, the implants were removed after 4 and 8 weeks. Thin sections were cut from paraffin embedded tissue and histological sections were stained hematoxylin and eosin staining. Motor functional outcome measurements using the Basso-Beattie-Bresnehan (BBB) score performed weekly to 8 weeks postinjury. It was observed that the effects of the PGA/PF127 with BMSCs on neuroinduction (Group III, tissue engineered) are stronger that PGA/PF127 without BMSCs (Group II, matrix control) and blank control (Group I). In conclusion, these results suggest that BMSCs may have an important role for spinal cord regeneration of tissue engineering area.