慶南 島嶼地域 井戶에 對한 環境衛生學的 調査硏究

鄭文植,李弘根,李容旭 서울大學校 保健大學院 1972 公衆保健雜誌 Vol.9 No.1

A study was carried out to find the sanitary condition of wells in Koje Island, Kyoungnam Province, from July 16, to July 30, 1971. Salinity was measured to check the seepage of sea water by the silver nitrate titration method and coliform groups were examined by the membrane filter method as well as the general sanitary state of wells and their surroundings. Main findings were as follows; 1. the average chloride ion concentration of 41 wells examined was 54.0ppm with the standard deviation of 31.8 which was below the maximum permissible concentration of 150.0ppm in drinking water. 2. the mean chloride ion at lower district wells was the highest of 69.9 ppm which looked to be influenced by the sea water. 3. no relation was found between the concentration of chloride ion and the pollution of wells which seemed to be more affected by the sanitary state of wells and their surroundings. 4. coliform groups were positive at 25 wells(61.0%) out of 41 and at 17 out of 21 of less than 2 m depth and no coliforms were found at the wells of more than 3 m one. Water-proof structure of 3 m depth was necessary to protect wells from pollution. 5. coliforms were found at all 15 wells within the 6 m distance from the pollution sources, but 3 out of 13 of more than 10 m one. 6. the rates of coliform positives were 92.8 percent at higher districts, 37.8 at lower ones and 27.2 at coastal ones.

골반경에 의한 자궁경부암 근치술 및 양측 골반 임파절 절제술 1례

김홍배,김중환,문종수,이재창,허민 대한산부인과내시경학회 1992 대한산부인과내시경학회잡지 Vol.4 No.2

금속도금공장 폐수중의 구리 및 시안화물의 효과적인 처리방법

諸元穆,洪鍾斗,金冕燮 漢陽大學校 環境科學硏究所 1988 環境科學論文集 Vol.9 No.-

구리 및 시안화물이 혼합된 도금폐수를 적절하게 처리하는 방법을 알기 위하여 pH,농도 및 접촉시간에 따르는 용액중 구리 및 시안화물의 제거율을 실험하였다. 그 결과 시안화물은 5% NaOC1용액으로 처리하면 잔류시안 농도가 0.03 ㎎/ℓ이하로 환경배출기준에 적합하였다. pH에 따르는 영향은 pH8 이상에서 99%제거율을 나타냈다. 구리혼합용액은 중화침전법으로 실험한 결과 pH8에서 99%가 짧은 시간에 제거되었다. 시안화물과 구리혼합용액의 pH에 따르는 제거율은 pH8 및 11에서 높은 제거율(95%이상)을 보였으며 pH10에서 제거율이 떨어지는 결과를 얻었다. To investigate the effective removal method of copper and cyanide compounds in metal plating waste water, removal ratio of copper and cyanide compounds in solution are measured with varying pH, concentration and contact time. As results of the present expriment, cyanide compounds in the solution are removed to 0.03 ㎎/ℓ or less with 5% NaOC1 solution. The present result is satisfied to environmental disposal standards. The removal ratio against pH values show 99% over pH8. As results of neutral precipitation method, copper including solution are removed to 99% at pH8 in short time. The removal ratios of cyanide mixed copper solution against pH values show high efficiency(over 95%) at pH8 and 11 and removal ratios are decreased at pH10.

Construction of Glycerol Synthesis Pathway in Klebsiella pneumoniae for Bioconversion of Glucose into 1,3-Propanediol

Hong Zong,Xiaohong Liu,Wenqiang Chen,Bin Zhuge,Jin Sun 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.5

1,3-Propanediol (1,3-PDO) is an important three-carbon compound widely used in new polyester polymer materials. Natural organisms that can produce 1,3- PDO from glycerol were well studied. However, no natural microorganisms found could directly convert glucose to 1,3-PDO due to its insufficient glycerol synthesis pathway. In this study, two essential glycerol synthesis genes, CgGPD gene (encoding glycerol-3-phosphate dehydrogenase from Candida glycerinogenes) and ScGPP2 gene (encoding glycerol-3-phosphatase from Saccharomyces cerevisiae), were expressed in wild-type Klebsiella pneumoniae, a natural 1,3-PDO producers with reduction pathway for 1,3- PDO synthesis from glycerol. The results of fermentation, key enzyme activities, and metabolites analysis confirmed that recombinant K. pneumoniae now possessed a metabolic pathway capable of converting glucose to 1,3-PDO. The strain could produce 1,3-PDO from glucose with a final titer of 17.27 g/L with 40 g/L glucose in the medium, showing a 1.26-fold increase compared with 30 g/L glucose. Also, adding certain concentrations of glycerol could quickly initiate the 1,3-PDO synthetic pathway and promote the accumulation of 1,3-PDO, which could shorten the fermentation cycle. These results have important implications for further studies involving the use of one strain for bioconversion of glucose to 1,3-PDO.

Cadmium induces apoptosis in primary rat osteoblasts through caspase and mitogen-activated protein kinase pathways

Hong-Yan Zhao,Wei Liu,Yi Wang,Nannan Dai,Jian-Hong Gu,Yan Yuan,Xue-Zhong Liu,Jian-Chun Bian,Zong-Ping Liu 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

Exposure to cadmium (Cd) induces apoptosis in osteoblasts (OBs); however, little information is available regarding the specific mechanismsof Cd-induced primary rat OB apoptosis. In this study, Cd reduced cell viability, damaged cell membranes and induced apoptosis in OBs. We observed decreased mitochondrial transmembrane potentials, ultrastructure collapse, enhanced caspase-3 activity, and increasedconcentrations of cleaved PARP, cleaved caspase-9 and cleaved caspase-3 following Cd treatment. Cd also increased the phosphorylationof p38-mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases (ERK)1/2 and c-jun N-terminal kinase (JNK) in OBs. Pretreatment with the caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone, ERK1/2 inhibitor (U0126), p38 inhibitor(SB203580) and JNK inhibitor (SP600125) abrogated Cd-induced cell apoptosis. Furthermore, Cd-treated OBs exhibited signs of oxidativestress protection, including increased antioxidant enzymes superoxide dismutase and glutathione reductase levels and decreased formationof reactive oxygen species. Taken together, the results of our study clarified that Cd has direct cytotoxic effects on OBs, which are mediatedby caspase- and MAPK pathways in Cd-induced apoptosis of OBs.

Possible role of Pax-6 in promoting breast cancer cell proliferation and tumorigenesis

( Xiang Yun Zong ),( Hong Jian Yang ),( Yang Yu ),( De Hong Zou ),( Zhi Qiang Ling ),( Xiang Ming He ),( Xu Li Meng ) 생화학분자생물학회 2011 BMB Reports Vol.44 No.9

Pax 6, a member of the paired box (Pax) family, has been implicated in oncogenesis. However, its therapeutic potential has been never examined in breast cancer. To explore the role of Pax6 in breast cancer development, a lentivirus based short hairpin RNA (shRNA) delivery system was used to knockdown Pax6 expression in estrogen receptor (ER)-positive (MCF-7) and ER-negative (MDA-MB-231) breast cancer cells. Effect of Pax6 silencing on breast cancer cell proliferation and tumorigenesis was analyzed. Pax6-RNAi-lentivirus infection remarkably downregulated the expression levels of Pax6 mRNA and protein in MCF-7 and MDA-MB-231 cells. Accordingly, the cell viability, DNA synthesis, and colony formation were strongly suppressed, and the tumorigenesis in xenograft nude mice was significantly inhibited. Moreover, tumor cells were arrested at G0/G1 phase after Pax6 was knocked down. Pax6 facilitates important regulatory roles in breast cancer cell proliferation and tumor progression, and could serve as a diagnostic marker for clinical investigation. [BMB reports 2011; 44(9): 595-600]

진공용 나노스테이지 개발

홍원표,강은구,이석우,최헌종 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

Miniaturization is the central theme in modem fabrication technology. Many of the components used in modern products are becoming smaller and smaller. The direct write FIB technology has several advantages over contemporary micromachining technology, including better feature resolution with low lateral scattering and capability of maskless fabrication. Therefore, the application of focused ion beam(FIB) technology in micro fabricaton has beome increasingly popular. In recent model of FIB, however the feeding system has been a very coarse resolution of about a few μm. It is not unsuitable to the sputtering and the deposition to make the high-precision structure in micro or macro scale. Our research is the development of nano stage of 200mm strokes and 10nm resolutions. Also, this stage should be effectively operating in ultra high vacuum of about 1×10^(-5)pa. This paper presents the concept of nano stages and the discussion of the material treatment for ultra high vacuum.

An Automatic Size Measuring Algorithm for SEM

Hong, Won-Pyo,Choi, Hon-Zong Korean Society for Precision Engineering 2015 International Journal of Precision Engineering and Vol.16 No.7

The SEM is a useful piece of equipment for measurement in the micro-nano region. In general, it has been usefully applied to measuring the shapes and properties of measurement specimens, but it has limitations in terms of its capacity to precisely measure size. In addition, an SEM may obtain different measurement results for the same specimen depending on the user. In this paper, an algorithm for automatic size measurement of electron beam images was proposed that uses an image processing technique. The experiment was performed on circular and rectangular shapes with diameters and widths ranging from 300 nm to 10 mm. Finally, the results were compared with the values measured by AFM equipment to verify the accuracy of the automatic size measurement.

Application of Computational Intelligence Techniques to an Environmental Flow Formula

Zong Woo Geem,Jin-Hong Kim 한국지능시스템학회 2018 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.18 No.4

Manning formula is one of the most famous functions used in hydraulics and hydrology, which calculates the average flow velocity based on roughness coefficient, hydraulic radius, and slope. This study intends to improve the original formula by minimizing the deviation error between calculated flow velocity and observed one. The first improvement approach was to estimate the exponent values of hydraulic radius and slope, instead of using current 2/3 and 1/2, while fixing the roughness value. When logarithm-converted multiple linear regression, calculus-based BFGS technique, and meta-heuristic genetic algorithm were applied to the problem, genetic algorithm found the best exponent values in terms of sum of squares error and coefficient of determination. The second approach was to estimate the individual roughness value, instead of a constant one, which is the function of hydraulic radius and slope. When multiple linear regression, artificial neural network with BFGS, and artificial neural network with genetic algorithm tackled the problem, the latter found the best solution. We hope these approaches will be utilized more practically in the future.

Development of an Enrichment Culture Growing at Low Temperature used for Ensiling Rice Straw

( Hong Yan Yang ),( Xiao Fen Wang ),( Li Juan Gao ),( Shin Haruta ),( Masaharu Ishii ),( Yasuo Igarashi ),( Zong Jun Cui ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.4