Chinese Students’ Identity Construction and Negotiation during Emergency Remote Teaching: A Case Study from Wuhan

Zilin Wang,Lucas Kohnke,Di Zou 아시아테플 2022 The Journal of Asia TEFL Vol.19 No.3

Identification and differential expression of microRNAs in Madin–Darby canine kidney cells with high and low tumorigenicities

Wang Jiamin,Liu Lixia,Yang Di,Zhang Li,Abudureyimu Ayimuguli,Qiao Zilin,Ma Zhongren 한국유전학회 2022 Genes & Genomics Vol.44 No.2

Background: Madin-Darby canine kidney (MDCK) cells are widely used for vaccine production, however, the safety of MDCK cells needs to be considered seriously because of high tumorigenicity. Micro RNAs (miRNAs) that are involved in the tumorigenicity of MDCK cells have been never been reported. Objective: To reveal the role of miRNA in the tumorigenic phenotype of MDCK cell line. Methods: The miRNA expression profiles of two monoclonal MDCK cells (M09CL and M35CL) with low tumorigenicity and one MDCK cell line (M73P) with high tumorigenicity were characterized and investigated by using small RNA-seq technology. Results: A total of 5 known miRNAs and 5 novel miRNAs were highly expressed in M73P. In addition, 4 known miRNAs and 4 novel miRNAs were highly expressed in M09CL and M35CL. The target genes of the differentially expressed miRNAs were significantly enriched in several biological processes, and the majority of these genes were involved in pathways in cancer and the MAPK signaling pathway. Through interaction analysis, 4 up-regulated miRNAs (cfa-miR-452, cfa-miR-8826, cfa-miR-224, and cfa-miR-2387) and their crucial target genes related to the tumor regulation network were identified. Results indicated these 4 miRNAs might play crucial roles in the tumorigenesis of MDCK cells. Conclusion: Our findings, which were based on the functional prediction of miRNAs and target genes, suggested that miRNAs might influence the tumorigenicity of MDCK cells by regulating target genes. Moreover, the results provided important data for understanding the miRNA-mediated regulatory networks that control the tumorigenicities of MDCK cells.

PATH FOLLOWING AND LATERAL-YAW-ROLL STABILITY INTEGRATED CONTROL METHOD FOR AUTONOMOUS DISTRIBUTED DRIVE ELECTRIC BUSES

Xin Zilin,Wang Wenwei,Liang Sheng 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.4

In order to improve the path following performance and solve the stability problems of autonomous distributed drive electric buses, this paper proposed a path following and lateral-yaw-roll stability integrated control method. Firstly, to cope with the varying speed during different maneuvers and roll motion of autonomous distributed drive electric buses, a nonlinear 4-DOF vehicle dynamic model which includes rolling and longitudinal movement is established. Secondly, a hierarchical control method is built, in the upper layer, a model predictive controller (MPC) is designed to generate the control inputs, which is obtained through an optimization problem including path following error and vehicle stability condition, especially the roll stability for buses, and for the lower layer, the torque of each drive wheel is distributed through solving an optimization problem about drive requirement and tire utilization. Finally, the validation of the proposed method is carried out through TruckSim-Simulink co-simulation, the results illustrate that both following accuracy and vehicle stability are obtained more effectively than the popular linear MPC method, especially roll stability is guaranteed under extreme condition.

Numerator-Denominator Model Based H<SUB>∞</SUB> Robust Control for LCL Filter-Based Grid-Connected Inverters under Weak Grid Conditions

Bo Long,Chu Wang,ZiLin Zhu,Xin Lu,LiJun Huang,YuFei Dai,Yong Liao,FuSheng Li 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

In the grid-connected inverter controller system, conventional PI or PR controller decrease their lowfrequency gain and bandwidth to maintain the stability of the system. However, this degrades the disturbance rejection and current tracking capability. To solve this problem, this paper gives an H∞ robust control scheme based on Numerator Denominator Model method, which has the advantages of simplified current controller design procedure, can place the poles of closed-loop system at the desired place. By properly selecting the weight function, the proposed control scheme can not only achieve good robustness in disturbance rejection capability, but also gives a straight forward to design the dynamic performance. Simulation and experimental comparison results between PR and H∞ controller is demonstrated. The results prove that the proposed control scheme shows obviously improvements on the robustness of inverter and meanwhile does not lose advantages on the dynamic performance.

Long non-coding RNA RP11-6O2.4 indicates poor prognosis and suppresses cell cycle progression through the p38-MAPK signaling pathway in gastric cancer

Yang Feng,Zhiming Fu,Yajun Luo,Wang Tan,Zilin Liu,Pengcheng Ye,Fei Lu,Wanping Xiang,Linghan Tang,Lin Yao,Mengyun Song,Qingmei Huang,Yilun Liu,Jiangwei Xiao 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.3

Backgrounds: The role of long non-coding RNAs (lncRNA) in gastric cancer (GC) has been highlighted in studies conducted over the past decade. However, the potential clinical value and the mechanisms of action of RP11-6O2.4 in GC have not been thoroughly elucidated to date. The specific aim of the present study was to assess RP11-6O2.4 and to explore its role in human GC. Methods: Quantitative real-time polymerase chain reaction (qPCR) was performed to analyze the expression levels of RP11-6O2.4 in GC tissues, paired adjacent noncancerous tissues (ANTs) and GC cell lines. In addition, the correlation between RP11-6O2.4 expression and the clinical characteristics and prognosis of patients with GC was statistically analyzed. The effects of RP11- 6O2.4 on the GC cell cycle transformation through the p38-MAPK signaling pathway were explored by flow cytometry, qPCR and Western blot analysis after treatment with SB203580, a p38MAPK specific inhibitor, in vitro. Results: The expression levels of RP11-6O2.4 in GC tissues were significantly lower than the paired ANTs (P<0.05). In addition, RP11-6O2.4 expression was significantly lower in cases with older age, longer maximum tumor diameter, higher ASA grade and deeper invasive depth (P<0.05). RP11-6O2.4 expression was significantly higher in cases with well/middle differentiation than poor/no differentiation; higher in cases without lymph node metastasis than in lymph node metastasis; and higher in cases in stage Ⅰ/Ⅱ than in stage Ⅲ/Ⅳ. An in vitro assay showed that RP11-6O2.4 induced G0/ G1 phase cell cycle arrest, likely by regulating the p38- MAPK signaling pathway. Conclusion: The above mentioned data suggested that RP11-6O2.4 was a tumor-suppressor gene in GC. RP11- 6O2.4 might play an important role in the cell cycle transformation by regulating the p38-MAPK signaling pathway, thereby representing a specific biomarker and a potential molecular target for the treatment of GC.

Microstructure and mechanical properties of yttrium aluminum garnet porous ceramics prepared by different sintering process parameters

Yue Liu,Xueqing Yang,Kangliang Peng,Qiong Wang,Jianzhen Huang,Zilin Zhang,Jiang Lu,Hao Xu,Jieguang Song,Lin Chen 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.4

Yttrium aluminum garnet (YAG), which possesses excellent properties, is investigated and applied. The sintering technology of YAG porous ceramics is optimized. Results show that the porosity initially increases and then decreases with an increase of increasing temperature rate, the porosity are decreased with an increase of removing carbon temperature, the porosity are decreased with an increase of sintering temperature, the porosity are decreased with an increase of holding time. Meanwhile, the compressive strength constantly exhibits an opposite tendency. The optimization of the sintering technology of YAG porous ceramics is based on porosity and compressive strength. A good sintering technology are an increasing temperature rate of 8 oC/min, a removing carbon temperature of 800 oC, removing carbon time of 1 h, a sintering temperature of 1450 oC and holding time of 2 h. The porosity of the prepared YAG porous ceramics is 57.4%, and the compressive strength is 8.89 MPa.

Prediabetes Progression and Regression on Objectively- Measured Physical Function: A Prospective Cohort Study

Qiu Shanhu,Yiming Zhu,Bo Xie,Wenji Chen,Duolao Wang,Cai Xue,Sun Zilin,Tongzhi Wu 대한당뇨병학회 2023 Diabetes and Metabolism Journal Vol.47 No.6

Background: Prediabetes leads to declines in physical function in older adults, but the impact of prediabetes progression or regression on physical function is unknown. This study assessed this longitudinal association, with physical function objectivelymeasured by grip strength, walking speed, and standing balance, based on the Health and Retirement Study enrolling United States adults aged >50 years.Methods: Participants with prediabetes were followed-up for 4-year to ascertain prediabetes status alteration (maintained, regressed, or progressed), and another 4-year to assess their impacts on physical function. Weak grip strength was defined as <26 kg for men and <16 kg for women, slow walking speed was as <0.8 m/sec, and poor standing balance was as an uncompleted fulltandem standing testing. Logistic and linear regression analyses were performed.Results: Of the included 1,511 participants with prediabetes, 700 maintained as prediabetes, 306 progressed to diabetes, and 505 regressed to normoglycemia over 4 years. Grip strength and walking speed were declined from baseline during the 4-year followup, regardless of prediabetes status alteration. Compared with prediabetes maintenance, prediabetes progression increased the odds of developing weak grip strength by 89% (95% confidence interval [CI], 0.04 to 2.44) and exhibited larger declines in grip strength by 0.85 kg (95% CI, –1.65 to –0.04). However, prediabetes progression was not related to impairments in walking speed or standing balance. Prediabetes regression also did not affect any measures of physical function.Conclusion: Prediabetes progression accelerates grip strength decline in aging population, while prediabetes regression may not prevent physical function decline due to aging.